HRP2 (HDGFRP2) (AK027353) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HDGF-2; HDGF2; HRP-2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK027353, the custom clone sequence may differ by one or more nucleotides AAGGTCGACAGCCCGGACGTGAAGAGGTGCCTGAATGCCCTAGAGGAGCTGGGAACCCTGCAGGTGACCT CTCAGATCCTCCAGAAGAACACAGACGTGGTGGCCACCTTGAAGAAGATTCGCCGTTACAAAGCGAACAA GGACGTAATGGAGAAGGCAGCAGAAGTCTATACCCGGCTCAAGTCGCGGGTCCTCGGCCCAAAGATCGAG GCGGTGCAGAAAGTGAACAAGGCTGGGATGGAGAAGGAGAAGGCCGAGGAGAAGCTGGCCGGGGAGGAGC TGGCCGGGGAGGAGGCCCCCCAGGAGAAGGCGGAGGACAAGCCCAGCACCGATCTCTCAGCCCCAGTGAA TGGCGAGGCCACATCACAGAAGGGGGAGAGCGCAGAGGACAAGGAGCACGAGGAGGGTCGGGACTCGGAG GAGGGGCCAAGGTGTGGCTCCTCTGAAGACCTGCACGAGTGAGTGTCCCGGGCCGTGGGGTTTGGACTCC TGAGCGGCAGCGGTGTGACGCGCACCCTGGGTCCGAGCCGCTCCTCCTGTGCCAGTCCCTCTGGGATGGG TCCCAGGGATGTCGTCCTTACTCGGGCCTCCCACCTTCACAGCTGACCCCAGGGCCCCGCTGGCTGGCAC TTCCGGCGGCCCCTACAGAGAGGCAGCTCCAGGGCTTTGAGCTGCCTGCCCCTGGCCTCCTGGGAAGGTG GCTTCTTGCCGAGCCTCCAGGGTGGCCCAGGTAGGTAGGCCCCGAGCACAGGCACCCGGCTATCTGACGG TGCCTGTGCCCATCACTGTGGGCTGCTGTCTGGTACAAGGCTCAGTGGAGTCACTCACTTACCCAGCAGC TGCCTTGTGCTATGCCTGGCTGGCGCCAGCGTGGGGACCCTGGAGATGGTTGTGGTCTCTAGTGGCAGAA GACAGGCAGATAGGAGGGCGGCACTCGAGCCTCAGGTGCCACAACGGGGGTACACTCCTCGGTGCCCATC CCACCCAACCGCCCTACAGGCAGGGCAGGGGCGGGAGGGCATCCTCACACCGCCTTTGCTGTTCCCACCA GCAGCGTACGGGAGGGTCCCGACCTGGACAGGCCTGGGAGCGACCGGCAGGAGCGCGAGAGGGCACGGGG GGACTCGGAGGCCCTGGACGAGGAGAGCTGAGCCGCGGGCAGCCAGGCCCAGCCCCCGCCCGAGCTCAGG CTGCCCCTCTCCTTCCCCGGCTCGCAGGAGAGCAGAGCAGAGAACTGTGGGGAACGCTGTGCTGTTTGTA TTTGTTCCCTTGGGTTTTTTTTTCCTGCCTAATTTCTGTGATTTCCAACCAACATGAAATGACTATAAAT GGTTTTTTAATGAAAAAAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK027353 unedited CCAGGAAACCTGGCCAGAAGGAGAAGAGAGTGCGGCCCGAGGAGAAGCAACAAGCCAAGC CCGTGAAGGTGGAGCGGACCCGGAAGCGGTCCGAGGGCTTCTCGATGGACAGGAAGGTAG AGAAGAAGAAAGAGCCCTCCGTGGAGGAGAAGCTGCAGAAGCTGCACAGTGAGATCAAGT TTGCCCTAAAGGTCGACAGCCCGGACGTGAAGAGGTGCCTGAATGCCCTAGAGGAGCTGG GAACCCTGCAGGTGACCTCTCAGATCCTCCAGAAGAACACAGACGTGGTGGCCACCTTGA AGAAGATTCGCCGTTACAAAGCGAACAAGGACGTAATGGAGAAGGCAGCAGAAGTCTATA CCCGGCTCAAGTCGCGGGTCCTCGGCCCAAAGATCGAGGCGGTGCAGAAAGTGAACAAGG CTGGGATGGAGAAGGAGAAGGCCGAGGAGAAGCTGGCCGGGGAGGAGCTGGCCGGGGAGG AGGCCCCCCAGGAGAAGGCGGAGGACAAGCCCAGCACCGATCTCTCAGCCCCAGTGAATG GCGAGGCCACATCACAGAAGGGGGGAGAGCGCANAGGACANGGAGCACGAGGAGGGTCGG NNACTCGAGGANGGGCCCAAGGTGTNGGCTCTCTNGAGACCTGCACGACAGCGTACGGGN AGGGTCCCGACCTGGACAGGCCCTGGANCNACCCGCAGACNCNANAGGGCACGGGGGACT CNNAGCCCTGACGAGAGANTGACCNCGGGCANCAGGCCANCCCCGCCGACTAGGNTGCCC TTTCTTTTTCCGGTGAGAAACAAACNAAACTGGGGGACGCCGGCCGNNGGTTNGCCCCCT GGGTGTTTTTCCGCCAATTTGGGAG
Restriction Sites	NotI-NotI
ACCN	AK027353
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK027353.1
RefSeq Size	1349 bp
RefSeq ORF	1349 bp
Locus ID	84717
Gene Summary	This gene encodes a member of the hepatoma-derived growth factor (HDGF) family. The protein includes an N-terminal PWWP domain that binds to methyl-lysine-containing histones, with specific binding of this protein to tri-methylated lysines 36 and 79 of histone H3, and di- and tri-methylated lysine 20 of histone H4. The protein functions in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]

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