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VARP (ANKRD27) (NM_032139) Human Untagged Clone
CAT＃: SC105947
ANKRD27 (untagged)-Human ankyrin repeat domain 27 (VPS9 domain) (ANKRD27)
CNY 10,176.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PP12899; VARP |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC105947 sequence for NM_032139 edited (data generated by NextGen Sequencing)
ATGGCTCTGTATGATGAAGACCTCCTGAAAAATCCTTTCTATCTGGCTCTGCAAAAGTGC CGCCCTGACTTGTGCAGCAAAGTGGCCCAAATCCATGGCATTGTCTTAGTACCCTGCAAA GGAAGCCTGTCGAGCAGCATCCAGTCTACTTGTCAGTTTGAGTCCTACATTTTGATACCT GTGGAAGAGCATTTTCAGACCTTAAATGGAAAGGATGTCTTTATTCAAGGGAACAGGATT AAATTAGGAGCTGGTTTTGCCTGTCTTCTCTCAGTGCCCATTCTCTTTGAAGAAACTTTC TACAATGAAAAAGAAGAGAGTTTCAGCATCCTGTGTATAGCCCATCCTTTGGAAAAGAGA GAGAGTTCAGAAGAGCCTTTGGCACCCTCAGATCCCTTTTCCCTGAAAACCATTGAAGAT GTGAGAGAGTTCTTGGGAAGACACTCCGAGCGATTTGACAGGAACATCGCCTCTTTCCAT CGAACATTCCGAGAATGCGAGAGAAAGAGCCTCCGTCACCACATAGACTCAGCGAATGCT CTCTACACCAAATGCCTCCAGCAGCTTCTGAGGGACTCTCACCTGAAAATGCTCGCCAAG CAGGAGGCCCAGATGAACCTGATGAAGCAGGCAGTGGAGATATACGTCCATCATGAAATT TACAACCTGATCTTTAAATACGTGGGGACCATGGAGGCAAGTGAGGATGCGGCCTTTAAC AAAATCACAAGAAGCCTTCAAGATCTTCAGCAGAAAGATATTGGTGTGAAACCGGAGTTC AGCTTTAACATACCTCGTGCCAAAAGAGAGCTGGCTCAGCTGAACAAATGCACCTCCCCA CAGCAGAAGCTTGTCTGCTTGCGAAAAGTGGTGCAGCTCATTACACAGTCTCCAAGCCAG AGAGTGAACCTGGAGACCATGTGTGCTGATGATCTGCTATCAGTCCTGTTATACTTGCTT GTGAAAACGGAGATCCCTAATTGGATGGCAAATTTGAGTTACATCAAAAACTTCAGGTTT AGCAGCTTGGCAAAGGATGAACTGGGATACTGCCTGACCTCATTCGAAGCTGCCATTGAA TATATTCGGCAAGGAAGCCTCTCTGCTAAACCCCCTGAGTCTGAGGGATTTGGAGACAGG CTGTTCCTTAAGCAGAGAATGAGCTTACTCTCTCAGATGACTTCGTCTCCCACCGACTGC CTGTTTAAGCACATTGCATCAGGTAACCAGAAAGAAGTGGAGAGACTTCTGAGCCAAGAG GACCATGATAAAGATACCGTCCAAAAGATGTGTCACCCTCTCTGCTTCTGCGATGACTGT GAGAAACTCGTCTCTGGGAGGTTGAATGATCCCTCAGTTGTCACTCCATTCTCCAGAGAC GACAGGGGGCACACCCCTCTCCATGTGGCTGCTGTCTGTGGGCAGGCATCCCTCATCGAC CTCCTGGTTTCCAAGGGCGCCATGGTAAATGCCACAGACTACCATGGGGCCACTCCGCTC CACCTGGCCTGTCAGAAGGGCTACCAGAGCGTGACGCTGCTGCTGCTGCACTACAAGGCC AGCGCGGAAGTGCAGGACAACAATGGGAATACGCCACTCCACCTGGCCTGCACCTACGGC CACGAGGACTGTGTGAAGGCTCTGGTTTACTACGACGTGGAGTCGTGCAGACTTGACATT GGCAATGAGAAAGGAGACACCCCTCTACACATTGCTGCCCGCTGGGGCTACCAAGGCGTC ATAGAGACATTGCTGCAGAACGGAGCGTCCACCGAGATCCAGAACAGACTGAAGGAGACG CCCCTCAAGTGTGCATTAAACTCAAAGATTCTGTCTGTAATGGAAGCCTATCACCTGTCC TTCGAGAGGAGGCAGAAGTCGTCCGAGGCCCCTGTGCAGTCCCCGCAGCGCTCCGTGGAC TCCATCAGCCAAGAGTCCTCCACTTCCAGCTTCTCCTCCATGTCAGCCAGCTCAAGGCAG GAGGAGACCAAGAAGGACTACAGAGAGGTAGAAAAACTTTTGAGAGCAGTTGCTGATGGA GATCTAGAAATGGTGCGTTACCTGTTGGAATGGACAGAGGAGGACCTGGAGGATGCGGAG GACACTGTCAGTGCAGCGGACCCCGAATTCTGTCACCCGTTGTGCCAGTGCCCCAAGTGT GCCCCAGCTCAGAAGAGGCTGGCGAAGGTTCCTGCCAGTGGGCTTGGTGTGAACGTGACC AGCCAGGACGGCTCCTCCCCGCTGCATGTCGCCGCCCTGCACGGCCGGGCGGACCTCATC CCCCTCCTGCTGAAGCACGGGGCCAACGCAGGTGCCAGGAACGCAGACCAAGCCGTCCCG CTCCACCTGGCCTGCCAGCAGGGCCACTTTCAGGTGGTGAAGTGTCTGTTAGATTCGAAT GCAAAACCCAATAAGAAGGACCTCAGTGGAAACACGCCCCTCATTTACGCCTGCTCCGGT GGCCATCACGAGCTTGTGGCACTGCTGCTACAGCACGGGGCCTCCATTAACGCTTCTAAC AATAAGGGCAACACAGCGCTGCACGAGGCTGTGATTGAAAAGCACGTCTTCGTGGTAGAG CTGCTTCTGCTCCACGGAGCGTCAGTTCAGGTGCTGAACAAGCGGCAGCGCACGGCTGTA GACTGTGCTGAACAGAATTCAAAAATAATGGAATTGCTTCAGGTGGTACCAAGCTGTGTT GCTTCATTAGATGATGTGGCTGAAACTGACCGCAAGGAGTATGTCACTGTTAAGATCAGG AAAAAATGGAACTCAAAACTGTATGATCTACCAGATGAGCCTTTTACAAGACAGTTTTAC TTTGTCCACTCAGCTGGTCAGTTTAAGGGAAAGACTTCAAGGGAGATTATGGCAAGAGAT AGAAGTGTCCCTAATTTAACCGAAGGTTCTTTGCATGAGCCAGGGAGGCAAAGTGTCACA CTGAGACAGAATAACCTGCCAGCTCAGAGTGGATCTCATGCTGCTGAGAAAGGCAACAGC GACTGGCCAGAGAGGCCTGGACTGACACAGACTGGCCCTGGACACAGACGGATGCTGCGG AGACACACGGTAGAGGATGCGGTCGTGTCCCAGGGCCCGGAGGCTGCTGGCCCCCTCTCC ACTCCCCAAGAGGTTAGTGCTTCCCGGTCCTAA Clone variation with respect to NM_032139.2 1488 a=>g >OriGene 5' read for NM_032139 unedited
GGTCACAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTGGGCTAT AGGGCCAGATCGCCGCGCGAGGGTGGTGGGCATCGAGGTCCCAGCAGCGGACGAGGGAGG TGCCGCCGTCGCCCAGGATGGGCTGGGAATGAAGCGATGTAGCCTTTTAAGAGATTTGCT CTGACCCATCTGAAGTCCATATGGCTCTGTATGATGAAGACCTCCTGAAAAATCCTTTCT ATCTGGCTCTGCAAAAGTGCCGCCCTGACTTGTGCAGCAAAGTGGCCCAAATCCATGGCA TTGTCTTAGTACCCTGCAAAGGAAGCCTGTCGAGCAGCATCCAGTCTACTTGTCAGTTTG AGTCCTACATTTTGATACCTGTGGAAGAGCATTTTCAGACCTTAAATGGAAAGGATGTCT TTATTCAAGGGAACAGGATTAAATTAGGAGCTGGTTTTGCCTGTCTTCTCTCAGTGCCCA TTCTCTTTGAAGAAACTTTCTACAATGAAAAAGAAGAGAGTTTCAGCATCCTGTGTATAG CCCATCCTTTGGAAAAGAGAGAGAGTTCAGAAGAGCCTTTGGCACCCTCAGATCCCTTTT CCCTGAAAACCATTGAAGATGTGAGAGAGTTCTTGGGAAGACACTCCGAGCGATTTGACA GGAACATCGCCTCTTTCCATCGAACATTCCGAGAATGCGAGAGANAGAGCCTCCGTCACC ACATAGACTCAGCGAATGCTCTCTACACCAAATGCCTNCAGCAGCTTCTGAGGGACTCTC ACCTGANAATGCTCNGCCAGCANNGAGCCCAGATGAACCTGATGAAGCAGGCAGTGGAGA TATACGTCCATCATGAAATTACCACCTGATCTTTAATACGTGGGGACATGGNAGCAGTGA GGATGCGGGCTTTACAC >OriGene 3' read for NM_032139 unedited
TATGGACCGCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTAAAAACATAAA GTTTACTGTTTCTTTTCCATCATTTGGCACATATCCAAGGCACATTAGAAAATTAGAAAT CATAAATTACTTTGTAGAAAAATAATCCCTCCCTTCCTTCTGTACATACACAAGTGTTTC CAAGAACATGGACAAAACCATTTCCCTATCACAAGGTCATTTGAAAACGGACTCAGGACA AACCCATATACGTGTAGCTCTAGGCCAATAACATAAAAATAACAGTATAATCTATAGAAA TTTATAAAAAGGAATAAATGGCAATAAATTCTAACCGAAAGTAACTCTGACCTGGTTTGT GCTGTTAAGTTTTGAATTTGAATCAAAAGACTAAGAAAAGATGTGCCAAATCTACTCTCT AATCCAGTAACCATCCCGTCAGGTGTGAGTGTGACAGACATTCAAGGGAGGTTCTCAACA AAAAGGGATGTTTCTAGAAATACTGCTACAGGTGCACGTGTAGTAACCGAATATCTGTAC CTACTTGTTAGAAGGAAAATGATAACCACCCTTTCCCATGTGCATGATGTGGAACAGGTC TGATGCATCCTTTCACTAAAATGAATATCAAAATGGGATTGAGGCATCTTTTGGTTCGAG TGTTGCTTTGCAAAGAAACATTGTCCAGCTGCTGCTTTCTTCCTTCAGATATGACATTTC AGCCTGGCCTCTACAGTACCATGAAACCTAAATTACTTACTTCTCTCCAGCTTAATCCTA AGTGGAGGAATTCTANCTGGGGCTTGTGAATGTACACGGTCANAGAAGTTTANCACCTTT GGGAGGGAGAGGCAAATGCCGNGGTGAAACCTTTCCACTGTGACAACAACCCTATACTAA AAGACCATGCCCCTTGCTNAAGGACTGGAGCTCTGGGATTTTTGCCGGTCCAATGATTCA TACCTTGAAAGGAAAGAAGGGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032139 |
| Insert Size | 4640 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032139.1, NP_115515.1 |
| RefSeq Size | 4501 bp |
| RefSeq ORF | 4461 bp |
| Locus ID | 84079 |
| UniProt ID | Q96NW4 |
| Domains | ANK, VPS9 |
| Gene Summary | May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed:16525121, PubMed:18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed:23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed:22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (PubMed:19745841). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (PubMed:24856514). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208500 | ANKRD27 (Myc-DDK-tagged)-Human ankyrin repeat domain 27 (VPS9 domain) (ANKRD27) |
CNY 7,312.00 |
|
| RC208500L1 | Lenti ORF clone of Human ankyrin repeat domain 27 (VPS9 domain) (ANKRD27), Myc-DDK-tagged |
CNY 9,712.00 |
|
| RC208500L2 | Lenti ORF clone of Human ankyrin repeat domain 27 (VPS9 domain) (ANKRD27), mGFP tagged |
CNY 7,600.00 |
|
| RC208500L3 | Lenti ORF clone of Human ankyrin repeat domain 27 (VPS9 domain) (ANKRD27), Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC208500L4 | Lenti ORF clone of Human ankyrin repeat domain 27 (VPS9 domain) (ANKRD27), mGFP tagged |
CNY 7,600.00 |
|
| RG208500 | ANKRD27 (tGFP-tagged) - Human ankyrin repeat domain 27 (VPS9 domain) (ANKRD27) |
CNY 8,912.00 |
