CDK5RAP2 (BC019577) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C48; CDK5 activator-binding protein C48; CDK5 regulatory subunit associated protein 2; centrosomal protein 215 kDa; Cep215; DKFZp686B1070; DKFZp686D1070; KIAA1633; MCPH3; OTTHUMP00000022800; OTTHUMP00000064031
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC019577, the custom clone sequence may differ by one or more nucleotides GATGGCGGCGTCTGTGGTTTGAATTCCAGCGGCGCCGCCAGAGTCTGAACAAGAGCTGGGGTGGAGGGGG CGGGGACCTGGGGAGCCCGGCGGGTCGCTATCGCGGGGGGTACTAGTGGCGCCGCCGCCACAGACACCAA CGCCGTCGCCACCTCTGTATCCATGATGGACTTGGTGTTGGAAGAGGACGTCACCGTCCCTGGGACGCTC AGCGGCTGCAGTGGCCTTGTTCCCAGTGTACCAGATGACCTGGATGGCATCAACCCCAATGCTGGGTTGG GAAATGGTCTGCTCCCAAATGTGTCAGAAGAAACAGTGTCTCCCACCAGAGCACGGAACATGAAGGACTT TGAAAATCAAATCACTGAATTGAAGAAAGAAAACTTTAACCTAAAGCTCCGCATCTATTTCCTTGAGGAA AGAATGCAACAGGAATTTCATGGCCCCACTGAACATATCTACAAAACTAACATTGAGCTCAAGGTGGAAG TAGAAAGTCTGAAGCGGGAACTCCAGGAGAGAGAGCAGCTGCTCATCAAAGCCTCCAAAGCAGTTGAGAG CTTAGCTGAAGCAGGTGGCTCTGAAATCCAGCGGGTGAAAGAAGATGCTCGAAAGAAGGTGCAGCAGGTG GAAGATCTCCTAACTAAAAGAATACTCCTTTTGGAAAAGGATGTGACAGCCGCCCAGGCAGAACTGGAAA AGGCCTTTGCAGGGACAGAGACGGAGAAGGCTCTTCGGTTGCGTTTGGAAAGCAAGCTTTCAGAGATGAA GAAGATGCACGAGGGGGACTTGGCGATGGCTCTGGTCCTGGATGAGAAAGACAGACTGATTGAGGAGTTG AAGCTGTCTTTGAAGAGCAAAGAAGCTTTAATTCAGTGCCTTAAAGAGGAGAAATCTCAGATGGCATGTC CTGATGAGAATGTGTCATCTGGAGAGCTCCGAGGACTTTGTGCTGCTCCAAGGGAAGAAAAGGAGAGAGA AACTGAGGCTGCACAAATGGAGCATCAGAAGGAGAGAAACAGCTTTCAAGAGAGGATCCAGGCACTTGAA GAGGACCTGAGAGAGAAGGAAAGAGAAATTGCTACAGAGAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC019577 unedited GTTCAGAATAGTATACGACTTATATAGGCGGCCGCGAAATTCGCACGAGGGAAAGAGGCG GCGTCTGTGGTTTGAATTCCAGCGGCGCCGCCAGAGTCTGAACAAGAGCTGGGGTGGAGG GGGCGGGGACCTGGGGAGCCCGGCGGGTCGCTATCGCGGGGGGTACTAGTGGCGCCGCCG CCACAGACACCAACGCCGTCGCCACCTCTGTATCCATGATGGACTTGGTGTTGGAAGAGG ACGTCACCGTCCCTGGGACGCTCAGCGGCTGCAGTGGCCTTGTTCCCAGTGTACCAGATG ACCTGGATGGCATCAACCCCAATGCTGGGTTGGGAAATGGTCTGCTCCCAAATGTGTCAG AAGAAACAGTGTCTCCCACCAGAGCACGGAACATGAAGGACTTTGAAAATCAAATCACTG AATTGAAGAAAGAAAACTTTAACCTAAAGCTCCGCATCTATTTCCTTGAGGAAAGAATGC AACAGGAATTTCATGGCCCCACTGAACATATCTACAAAACTAACATTGAGCTCAAGGTGG AAGTAGAAAGTCTGAAGCGGGAACTCCAGGAGAGAGAGCAGCTGCTCATCAAAGCCTCCA AAGCAGTTGAGAGCTTAGCTGAAGCAGGTGGCTCTGAAATCCAGCGGGTGAAAGAAGATG CTCGAAAGAAGGTGCAGCAGGTGGAAGATCTCCTCACTAAAAGAATACTCCTTTTGGAAA AAGATGTGACAGCCGCCCCAGCAGAACTGGAAAAGCCTTTGCAGGNACAGAGACCGAGAA GGCTCTTCGGTTGCGTTTGGAAAGCAGCTTTCACAGATGAAGAAGTGCACGAGGGGGACT TNGCGATGGCTCTGGTCCTGGATGAAAAAGAAGACTGATTTGAGGAGTGAAGCTGTCTTT GAGAGCAAGAAGCTTTAATTCATGCCTTAAG
Restriction Sites	NotI-NotI
ACCN	BC019577
Insert Size	5500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019577.1, AAH19577.1
RefSeq Size	1104 bp
RefSeq ORF	1104 bp
Locus ID	55755
Gene Summary	This gene encodes a regulator of CDK5 (cyclin-dependent kinase 5) activity. The protein encoded by this gene is localized to the centrosome and Golgi complex, interacts with CDK5R1 and pericentrin (PCNT), plays a role in centriole engagement and microtubule nucleation, and has been linked to primary microcephaly and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

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