UBTF (AF289595) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NOR-90; UBF; UBF-1; UBF1; UBF2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF289595, the custom clone sequence may differ by one or more nucleotides ATGGGGGAAGAGCGGGAACTCTGCCACGGAGGTGGAGCAAGAACGGTGAAGGGCCCTGGTCCCAGAGAGG CTGGTGGGTCCCTCTCCCAAAGGAAGGCAGACAGTCTCTGCTTTGCCTTGGACCTTGGTGCTTGGGGGTG GGGAGGCCCTGGGGGGGACACTCCCCACTCCCATTCCCCTTCCTTTGTCCTAATCCTGGAATTAAGTACA GGGGTTTATAGGTTCTATTTCTTCCCAAGAGCCCTGCAAAGAACCCCAGTTTCCTATTTGGATGCCCCTA CACTGTTGTGTTTCAGTGGAATGTATTTTCATTTAAAAACAACTTTGAATGGGGCACTTTTTCTTTCCTG TTTTAAAAATTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF289595 unedited NGGTTTCGGATTTTGTAACACGATTCACTATAGGGCGGACCGCGAATTCGCACNGGGGGT TCTTTTTTATGGTCATGTCACTGACCTATTAAATTGGGGCTTGGTGCTTTTCCACCTTCC CCCTCTGAATGAAAGCCAAGGAATGGGGGAAGAGCGGGAACTCTGCCACGGAGGTGGAGC AAGAACGGTGAAGGGCCCTGGTCCCAGAGAGGCTGTTGGGTCCCTCTCCCAAAGGAAGGC AGACAGTCTCTGCTTTGCCTTGGACCTTGGTGCTGGGGGTGGGGAGGCCTGGGGGGGACA CTCCCCACTCCCATTCCCCTTCCTTTGTCCTAATCCTGGAATTAAGTACAGGGGTTTATA GGTTCTATTTCTTCCCAAGAGCCCTGCAAAGAACCCCAGTTTCCTATTTGGATGCCCCTA CACTGTTGTGTTTCAGTGGAATGTATTTTCATTTAAAAACAACTTTGAATGGGGCACTTT TTCTTTCCTGTTTTAAAAATTGAAAAATTCTTACAGTACAAACAGGACTGTCAGGGTGGG GGTGTTGGTGCTGTAAGAGGTCACTCTTGAGTGCATTTTGGCACTGGGATGGGATGGTGG GGTGGGGGAANNCCNNNNNNNNNNNCCNNNNNNTNNNNTTNNNNTNTTNTNNGGNGTGTT TTTGGNGGGTTTNNCCNNNNNNNNNNNNTCTTGGTTTTTGTTTTNTTGTGGGGGGTGGGG GGGNGTTGGNTTTTNNGGTTGTNTTGCTTTGGTTTTNNGGGNNNTTGGTTGNNGTTTTTG TTTTTCCTTTTTTTGTTTTTTNNNGGTGGTTTTTNNTTGTTTTTTNNNNNNTTTTTGGGC TTTTTTTGGNGGGCCGNGTTTTTGTTGTTCTCTGTCCCNTTCCCGGGTGGCGTTCCTTGG NTCCCTCCCTCCGGCCTCCTTCTGGCCTCGTNGTTGCCCC
Restriction Sites	NotI-NotI
ACCN	AF289595
Insert Size	800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF289595.1, AAL55779.1
RefSeq Size	1545 bp
RefSeq ORF	1545 bp
Locus ID	7343
Protein Families	Transcription Factors
Gene Summary	This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]

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