ATP11B (AF156548) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATPIF; ATPIR
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF156548, the custom clone sequence may differ by one or more nucleotides GCCGCGGGATGGGAACGCGGCGCGGGGAGTGAGGCAGTGGCGGCGGCGGCGGTAAGCGGAACTTCGGCCC GAGGGGCTCGCCCGCTCCCGCCTCTGTCTTGTCGGCCTCCACCTGCAGCCCCGCGGCCCCCGCGCCCCGC GGGACCCGGACGGCGACGACGGGGGAATGTGGCGCTGGATCCGGCAGCAGCTGGGTTTTGACCCACCACA TCAGAGTGACACAAGAACCATCTACGTAGCCAACAGGTTTCCTCAGAATGGCCTTTACACACCTCAGAAA TTTATAGATAACAGGATCATTTCATCTAAGTACACTGTGTGGAATTTTGTTCCAAAAAATTTATTTGAAC AGTTCAGAAGAGTGGCAAACTTTTATTTTCTTATTATATTTTTGGTTCAGCTTATGATTGATACACCTAC CAGTCCAGTTACCAGTGGACTTCCATTATTCTTTGTGATAACAGTAACTGCCATAAAGCAGGGATATGAA GATTGGTTACGGCATAANTCAGATAATGAAGTAAATGGAGCTCCTGTTTATGTTGTTCGAAGTGGTGGCC TTGTAAAAACTAGATCAAAAAACATTCGGGTGGGTGATATTGTTCGAATAGCCAAAGATGAAATTTTTCC TGCAGACTTGGTGCTTCTGTCCTCAGATCGACTGGATGGTTCCTGTCACGTTACAACTGCTAGTTTGGAC GGAGAAACTAACCTGAAGACACATGTGGCAGTTCCAGAAACAGCATTATTACAAACAGTTGCCAATTTGG ACACTCTAGTAGCTGTAATAGAATGCCAGCAACCAGAAGCAGACTTATACAGATTCATGGGACGAATGAT CATAACCCAACAAATGGAAGAAATTGTAAGACCTCTGGGGCCGGAGAGTCTCCTGCTTCGTGGAGCCAGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF156548 unedited CGAATTCGGCACGAGGGCGGCAGGCTCAGCTGCGCCGGGCGGGGGCGGCGCCGGGGCCGC GCCTGTAGGACTCGGGGCCGACGCCGCGGGATGGGGACGCGGCGCGGGGAGTGAGGCAGT GGCGGCGGCGGCGGTAAGCGGAACTTCGGCCCGAGGGGCTCGCCCGCTCCCGCCTCTGTC TTGTCGGCCTCCACCTGCAGCCCCGCGGCCCCCGCGCCCCGCGGGACCCGGACGGCGACG ACGGGGGAATGTGGCGCTGGATCCGGCAGCAGCTGGGTTTTGACCCACCACATCAGAGTG ACACAAGAACCATCTACGTAGCCAACAGGTTTCCTCAGAATGGCCTTTACACACCTCAGA AATTTATAGATAACAGGATCATTTCATCTAAGTACACTGTGTGGAATTTTGTTCCAAAAA ATTTATTTGAACAGTTCAGAAGAGTGGCAAACTTTTATTTTCTTATTATATTTTTGGGTT CAGCTTATGATTGATACACCTACCAGTCCAGTTACCAGTGGACTTCCATTATTCTTTGTG ATAACAGTAACTGCCATAAAGCAGGGATATGAAGATTGGTTACGGCATAACTCAGATAAT GAAGTAAATGGAGCTCCTGTTTATGTTGTTCGAAGTGGTGGCCTTGTAAAACTAGATAAA AACATTCGGGTGGGTGATATTGTTCGATAGCCAAGATGAAATTTTTCTGCAGACTTGTGC TTCTGCCTCGATCACTGGATGGTTCTGTCACTACACTGCTA
Restriction Sites	NotI-NotI
ACCN	AF156548
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF156548.1, AAF09446.1
RefSeq Size	910 bp
RefSeq ORF	910 bp
Locus ID	23200
Protein Families	Transmembrane
Gene Summary	P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions, such as H(+), Na(+), K(+), or Ca(+). A third subfamily transports amphipaths, such as phosphatidylserine.[supplied by OMIM, Feb 2005]

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