MPRIP (AL834513) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	M-RIP; MRIP; p116Rip; RHOIP3; RIP3
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL834513, the custom clone sequence may differ by one or more nucleotides GACCTGACGCAGGCAAAACCCATTTATGGCGGTTGGCTGCTCCTGGCTCCAGATGGGACCGACTTTGACA ACCCAGTGCACCGGTCTCGGAAATGGCAGCGACGGTTCTTCATCCTTTACGAGCACGGCCTCTTGCGCTA CGCCCTGGATGAGATGCCCACGACCCTTCCTCAGGGCACCATCAACATGAACCAGTGCACAGATGTGGTG GATGGGGAGGGCCGCACGGGCCAGAAGTTCTCCCTGTGTATTCTGACGCCTGAGAAGGAGCATTTCATCC GGGCGGAGACCAAGGAGATCGTCAGTGGGTGGCTGGAGATGCTCATGGTCTATCCCCGGACCAACAAGCA GAATCAGAAGAAGAAACGGAAAGTGGAGCCCCCCACACCACAGGAGCCTGGGCCTGCCAAGGTGGCTGTT ACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCCCCAGTGCTGAGAAAGTCCCCACCACCAAGTCCA CACTCTGGCAGGAAGAAATGAGGACCAAGGACCAGCCAGATGGCAGCAGCCTGAGTCCAGCTCAGAGTCC CAGCCAGAGCCAGCCTCCTGCTGCCAGCTCCCTGCGGGAACCTGGGCTAGAGAGCAAAGAAGAGGAGAGC GCCATGAGTAGCGACCGCATGGACTGTGGCCGCAAAGTCCGGGTGGAGAGCGGCTACTTCTCTCTGGAGA AGACCAAACAGGACTTGAAGGCTGAAGAACAGCAGCTGCCCCCGCCGCTCTCCCCTCCCAGCCCCAGCAC CCCCAACCACAGGAGGTCCCAGGTGATTGAAAAGTTTGAGGCCTTGGACATTGAGAAGGCAGAGCACATG GAGACCAATGCAGTGGGGCCCTCACAATCCAGCGACACACGCCAGGGCCGCAGCGAGAAGAGGGCGTTCC CTAGGAAGCGGGACTTCACCAATGAAGCCCCCCCAGCTCCTCTCCCAGACGCCTCGGCTTCCCCCCTGTC TCCACACCGAAGAGCCAAGTCACTGGACAGGAGGTCCACGGAGCCCTCCGTGACGCCCGACCTGCTGAAT TTCAAGAAAGGCTGGCTGACTAAGCAGTATGAGGACGGCCAGTGGAAGAAACACTGGTTTGTCCTCGCCG ATCAAAGCCTGAGATACTACAGGGATTCAGTGGCTGAGGAGGCAGCCGACTTGGATGGAGAAATTGACTT GTCCGCATGTTACGATGTCACAGAGTATCCAGTTCAGAGAAACTATGGCTTCCAGATACATACAAAGGAG GGCGAGTTTACCCTGTCGGCCATGACATCTGGGATTCGGCGGAACTGGATCCAGACCATCATGAAGCACG TGCACCCGACCACTGCCCCGGATGTGACCAGCTCGTTGCCAGAGGAAAAAAACAAGAGCAGCTGCTCTTT TGAGACCTGCCCGAGGCCTACTGAGAAGCAAGAGGCAGAGCTGGGGGAGCCGGACCCTGAGCAGAAGAGG AGCCGCGCACGGGAGCGGAGGCGAGAGGGCCGCTCCAAGACCTTTGACTGGGCTGAGTTCCGTCTCATCC AGCAGGCCCTGGCTCAGGAGCGGGTGGGCGGCGTGGGGCCTGCTGACACCCACGAGCCCCTGCGCCCTGA GGCGGAGCCTGGGGAGCTGGAGCGGGAGCGTGCACGGAGGCGGGAGGAGCGCCGCAAGCGCTTCGGGATG CTCGACGCCACAGACGGGCCAGGCACTGAGGATGCAGCCCTGCGCATGGAGGTGGACCGGAGCCCAGGGC TGCCTATGAGCGACCTCAAAACGCATAACGTCCACGTGGAGATTGAGCAGCGGTGGCATCAGGTGGAGAC CACACCTCTCCGGGAAGAGAAGCAGGTGCCCATCGCGCCCGTCCACCTGTCTTCTGAAGATGGGGGTGAC CGGCTCTCCACACACGAGCTGACCTCTCTGCTCGAGAAGGAGCTGGAGCAGAGCCAGAAGGAGGCCTCAG ACCTTCTGGAGCAGAACCGGCTCCTGCAGGACCAGCTGAGGGTGGCCCTGGGCCGGGAGCAGAGCGCCCG TGAGGGCTACGTGCTGCAGGCCACGTGCGAGCGAGGGTTTGCAGCAATGGAAGAAACGCACCAGAAGAAG ATTGAAGATCTCCAGAGGCAGCACCAGCGGGAGCTAGAGAAACTTCGAGAAGAGAAAGACCGCCTCCTAG CCGAGGAGACAGCGGCCACCATCTCAGCCATCGAAGCCATGAAGAACGCCCACCGGGAGGAAATGGAGCG GGAGCTGGAGAAGAGCCAGCGGTCCCAGATCAGCAGCGTCAACTCGGATGTTGAGGCCCTGCGGCGCCAG TACCTGGAGGAGCTGCAGTCGGTGCAGCGGGAACTGGAGGTCCTCTCGGAGCAGTACTCGCAGAAGTGCC TGGAGAATGCCCATCTGGCCCAGGCGCTGGAGGCCGAGCGGCAGGCCCTGCGGCAGTGCCAGCGTGAGAA CCAGGAGCTCAATGCCCACAACCAGGAGCTGAACAACCGCCTGGCTGCAGAGATCACACGGTTGCGGACG CTGCTGACTGGGGACGGCGGTGGGGAGGCCACTGGGTCACCCCTTGCACAGGGCAAGGATGCCTATGAAC TAGAGGTCTTATTGCGGGTAAAGGAATCGGAAATACAGTACCTGAAACAGGAGATTAGCTCCCTCAAGGA TGAGCTGCAGACGGCACTGCGGGACAAGAAGTACGCAAGTGACAAGTACAAAGACATCTACACAGAGCTC AGCATCGCGAAGGCTAAGGCTGACTGTGACATCAGCAGGTTGAAGGAGCAGCTCAAGGCTGCAACGGAAG CACTGGGGGAGAAGTCCCCTGACAGTGCCACGGTGTCCGGATATGATATAATGAAATCTAAAAGCAACCC TGACTTCTTGAAGAAAGACAGATCCTGTGTCACCCGGCAACTCAGAAACATCAGGTCCAAGTCCGTAATT GAGCAGGTCTCGTGGGATACCTGAAATGCACCCGCTTCCCGGCCCATGCAGGAGAGTCTGAAGGAAGGCC TGACGGTGCAAGAACGGTTGAAGCTCTTTGAATCCAGGGACTTGAAGAAAGACTAGGTGTGTCCCATCCA AGTTGAGCACGCGCCTTCCCCAGCTTGCAGCAGCACACCCCAAGCGCTGCTTTTCACCTGTACCTTTGTT TTATTATTATTATTATTATTGCTGTTGTTGTCATCGTTAACTGTGGGCATGGAATGCGTGAGGCTGGCTT CTGGGTTGTCCACACCACTCTCTGCTGTGTTGACTTCCTGTTGTCTTCATCAAAGCTTTTTTCCGTGGTA TTCTAAAATTAGGCCAGCAGTGGGGGCTGGGAGGGCATCTGTGTTAGTCCTTTCCTGGCTGTGACCCGCC ACACTCACTGTCAGTATTAAGGCCCAGCAGCCTGTTGATAAGCTACCCTGTCTCACCATGTGCTGGTGTG GAAACGGGGCCCAGCCAGCACGCCTCAAGGTAGATGGAATCCCCACTGGTCAGAGAAAAAGCTATGCGGA CACTCCAGCTTGGCCTGGGTCACAGCACTGACTCCTCACCCGCTAGTCTGGCTGTTAAGAGGAGAAAGTG CACTGCCTTCCAGCCCAGGAGGAGGACAGCATTTTGTATTTGTTCCACTGATGCAGCTTAGAACCACACC CCTGAGAGTCGTGGCAAACCTTTCACAACCTGGAAAATGTTGAAAGCAACCATTCCTATTTTTGTTTGTT TTTTATTAAATCTTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANN 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL834513 unedited AGAGGCACTCATTTGTAAACCACTTCTATAGGCGGCCGCGNAATTCGCACGAGGCCGCCG CGCCGACCTGTCGGCAGCCAAGGAGAACCCGTGCAGGAAATTCCAGGCCAACATCTTCAA CAAGAGCAAGTGTCAGAACTGCTTCAAGCCCCGCGAGTCGCATCTGCTCAACGACGAGGA CCTGACGCAGGCAAAACCCATTTATGGCGGTTGGCTGCTCCTGGCTCCAGATGGGACCGA CTTTGACAACCCAGTGCACCGGTCTCGGAAATGGCAGCGACGGTTCTTCATCCTTTACGA GCACGGCCTCTTGCGCTACGCCCTGGATGAGATGCCCACGACCCTTCCTCAGGGCACCAT CAACATGAACCAGTGCACAGATGTGGTGGANTGGGGAGGGCCGCACGGGCCAGAAGTTCT CCCTGTGTATTCTGACGCCTGAGAAGGAGCATTTCATCCGGGCGGAGACCAAGGAGATCG TCAGTGGGTGGCTGGAGATGCTCATGGTCTATCCCCGGACCAACAAGCAGAATCAGAAGA AGAAACGGAAAGTGGAGCCCCCCACACCACAGGAGCCTGGGCCTGCCAAGGTGGCTGTTA CCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCCCCAGTGCTGAGAAAGTCCCCACCA CCAAGTCCACACTCTGGCAGGAAGAAATGAGGACCAAGGACCAGCCAGATGGCAGCAGCC TGAGTCCAGCTCAGAGTCCCAGCCAGAGCCAGCCTCCTGCTGCCAGCTCCCTGCGGGAAC CTGGGCTAGAGAGCAAAGAAGAGAGAGCGCCATGAGTAGCGACCGCATGGACTGTGGGCC GCAAGTCCGGGGTGGGAGAGCGCTACTTCTCTCTGGAGAAGACCAAACAGACTTGAAGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL834513 unedited TTTATGTACCGTCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTGGGTTTTGGGCCCAATTTAAAAAAAAACCACCCAAAAAGGGGGGGG GGGGTTTTAAAATTTTCCCAGGGGGGAAAAGGTTCCCCCCCCCCCCCGGGGGGGGGGTTT AAACCCCCCCCGGGGAAAAAAAAAAAAAAGGGGCCCCCCCCCCGGGGGGGAAAGGGGGGC CCTTTTTCCTTTTAAAACCCCAAAAAACGGGGGGGGAAACAAGTTTTTGCCCCCCCCCCA ACGGGGGGGGCCCCCAAATTTTTTTTTTCCCCCCGGGGGGTTCCCCCCCCCCTTGGGGGG GGGGGGGGGGCCCCCTTTTCCCCCCCCCCCCGGGGGGAAACGGGGGGTTTTTAAAACGGG GGGGGGGCCCTAAAAACAACAAGGGGGGGGGGGGGCCCCCCCCCCGAAAAGGAAAAAACA AAAGCCCCCCCCCCCCCCCCGGGGGGGCCTATTTTTAAAACCCCCCGAAAAAAACTTTTT TGAAAACCCCCGGGGGCCCCCCCCCCCAAAGGGGGGGGGGGCAACCCCAAAACCCCCCCC CCCCCTTTTTTGCCCCCGGTTAAAAAGGACCCCCCCCCCCCTTTTTTTTTTTTTTTTTAA ACCCAGGTCCCCGGGAAAAACCCCCCCTGGGGGGGGCCCCCCCCCACCGGGGAAAGGCGC CTCCCCACCTTTGGGGGGGACCCCCCCATTTTTTTTTAAAGCCCCGGGAAAAAAAAAGTT TAACCCGTTTTGCCCCCGCGGGCCCTCCTTTAAAACTTTGGACCCAAGTTTTTAGATTCC CCGGGGACAAAAATTCTTTTTTTTTTAAAAAAAACGGGGGCTTTTAT
Restriction Sites	NotI-NotI
ACCN	AL834513
Insert Size	4500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AL834513.1, CAD39169.1
RefSeq Size	3839 bp
RefSeq ORF	3839 bp
Locus ID	23164
Domains	PH
Protein Families	Druggable Genome
Gene Summary	Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.[UniProtKB/Swiss-Prot Function]

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