CCDC88C (AF070587) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DAPLE; HKRP2; KIAA1509; SCA40
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF070587, the custom clone sequence may differ by one or more nucleotides CGGGCGCGAGCCGCCGCAGCCCCGCGCCCGCTGCTCTCTGCGGAGCCGTTTTGTGCCGCGGCGCGGGGGA ACGGGGACGCGGGGCGGGGGGCGCGGAGCCGGCGGGCGCAGCCTCAGCATGGACGTGACAGTCTCGGAGC TCCTGGAGCTCTTCCTGCAGAGCCCGCTGGTGACCTGGGTGAAAACTTTTGGCCCGTTTGGAAGCGGCAG CCAGGACAACCTGACTATGTACATGGATTTAGTGGACGGCATCTTTTTGAACCAAATTATGCTGCAAATA GATCCCAGGCCCACAAATCAACGCATCAATAAGCACGTCAACAATGATGTGAACCTTCGCATTCAGAATT TGACCATCTTGGTGAGAAACATTAAGACCTACTACCAGGATAGACCCTTTTTCCGGTAACTAAACTTCTT GCGGGCAGACACAGCGTTTGAGTTTTCGCATTGGCTTCAAACAAACCCAGTGGAAGTGGACAGTGAACAA GACACCCCCGCAGGAGGCCCGGGCACGGGGTGTGGCTGCCAGGCCTGGCCATGGAAGGCCATCACTGTGG GCTCTTCTGGGAGTGGCACTGACAGCCAGCAAGGAGAATGGGTGCTGGGGCGGAGTGGAAGAAGGTGAGC TTGAGGCCGGAGTTCCTACCCCAGGAGGCACCGTCAGCGTCCCACTTCTGCTCGTGTGATCCCCTGGAGG CACCTGAATCAGGCAGTAAACATGGAGTGAGAAACACCGGAGTAGAAGACCTTGCAACAGCACATGTGTG GGCCCAGTTCCTAGCTGCCCCGGGTGGCCAAGGCCACATCCTGTACACACAGGTCTGGGAGTGTCTGCAT TTCAGCCCTTGACCGTGAACTTGACCCTCTGGAGGTGTCCATCTTGATGTGATCCTCTGACTGCAACCTC ATCCTCTGCCTCTCAGAGCTGCCTCCCCCTGTGGCTTCTTGCCCTGAGGCATGGGAGGCGTTGGTGCCAG GAGCTTGGGGTTTGAGTCAGGTTGGCTCGGGGATAAGACCCAGCCTTTCCACACATTAGTTTGTGATGCT GGGTCATAGTGCGTCTGTTTGCTCCCTGTCAAGGGACCAGTAACATGAGGGGTCAGAGAAGGGATCAGAA CTCTCGTGGGCCTCCAGTGTGTCGCAAGTTTTTGCTGTTCTGCTTTTCCTCAGCTGCTGGTGGGAATGCA AATTGCTGTGCTGACTTTGGAAAAACTCTCCGGCAGTAAAGCCTAAAGTTCCACATCCACACACCGTACT GTGGCCCAGCTGATTTCTCTCCTAAGGGTATCCCGGAGTAACTTCTGCACATGGATGCCTGGGACTTCAC AGCCCTTCTGCACACAATAACAGAAACCTGGAAGCCACCCAGATGCCCATCAGTGGGAGAAGGGATGAAT ACACGAAGACACGATCACACAATGGAATATTATACAGCTGTCCAAACACATTAACTGCAGCATAACTTCA GTGATGTAGGTGGACTCTGGCAATATCATAAATATTAGTAAAAATAGCATGTTCCCAATGATGACTTACA GCACTATGCCTTTTAAAAATAAAGTTAAAAGCAACTACAATGACTGTACTCTCTAGGAAAATTTACAGGT GCAATAAAACTACATAAAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF070587 unedited NCCCGTTCAAATTTGTATACGACTTACTATAGGCGGCCGCGAATTCGCACCAGGCGGAAC GTGGTCTCGCGCGCCCCGCCTTTGTCTCCCGGGCGCGAGCCGCCGCAGCCCCGCGCCCGC TGCTCGCTGCGGAGCCGTTTTGTGCCGCGGCGCGGGGGAACGGGGACGCGGGGCGGGGGG CGCGGAGCCGGCGGGCGCAGCCTCAGCATGGACGTGACAGTCTCGGAGCTCCTGGAGCTC TTCCTGCAGAGCCCGCTGGTGACCTGGGTGAAAACTTTTGGCCCGTTTGGAAGCGGCAGC CAGGACAACCTGACTATGTACATGGATTTAGTGGACGGCATCTTTTTGAACCAAATTATG CTGCAAATAGATCCCAGGCCCACAAATCAACGCATCAATAAGCACGTCAACAATGATGTG AACCTTCGCATTCAGAATTTGACCATCTTGGTGAGAAACATTAAGACCTACTACCAGGAT AGACCCTTTTTCCGGTAACTAAACTTCTTGCGGGCAGACACAGCGTTTGAGTTTTCGCAT TGGCTTCAAACAAACCCAGTGGAAGTGGACAGTGAACAAGACACCCCCGCAGGAGGCCCG GGCACGGNGGTGTGGCTGCCAGGCCTGGCCATGGAAGGCCATCACTGTGGACTCTTCTGG GAGTGGCACTGACAGCCAGCAGGAGAATGGGTGCTGGGGCGGAGTGGAAAAAGGTGAGCT TGAGGCCGGAGTTCCTACCCCAGGAGGCACCGTCAGCGTCCCACTTCTGCTCGTGTGATC CCCTGGNAGCACCTNGATCANGCAGTAACATGGAGTGAGAACACCGGAGTAGAAGACCTG CACAGCACATGTGTGGGCCCAGTNCTACTCCCCCGGNTGGCCAGGCCCATCTGTCCNCAG TTTGGGGAGGTCTGCATTCAGCTTTGACGGN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF070587 unedited NAAAGGTCCAACTATGNACCGCGGCACGCATTCTATGATCGATTTTTTTTTTTTTTTTTT ATGTAGTTTTATTGCACCTGTAAATTTTCCTAGAGATACAGTCATTGTAGTTGCTTTTAA CTTTATTTTTAAAAGGCATAGTGCTGTAAGTCATCATTGGGAACATGCTATTTTTACTAA TATTTATGATATTGCCAGAATCCACCTACATCACTGAAGTTATGCTGCAGTTAATGTGTT TGGACAGCTGTATAATATTCCATTGTGTGATCGTGTCTTCGTGTATTCATCCCTTCTCCC ACTGATGGGCATCTGGGTGGCTTCCAGGTTTCTGTTATTGTGTGCAGAAAGGCTGTGAAG TCCCAGGCATCCATGTGCAGAAGTTACTCCGGGATACCCTTAGGAGAGAAATCAGCTGGG CCACAGTACGGTGTGTGGATGTGGAACTTTAGGCTTTACTGCCGGAGAGTTTTTCCAAAG TCAGCACAGCAATTTGCATTCCCACCAGCAGCTGAGGAAAAGCAGAACAGCAAAAACTTG CGACACACTGGAGGCCCACGAGAGTTCTGATCCCTTCTCTGACCCCTCATGTTACTGGTC CCTTGACAGGGAGCAAACAGACGCACTATGACCCAGCATCACAAACTAATGTGTGGAAAG GCTGGGTCTTATCCCCGAGCCAACCTGACTCAAACCCCAAGCTCCTGGCACCAACGCCTC CCATGCCTCNAGGCAAGAAGCCACAGGGGGAGGCAGCTCTGANAGGCANAGGATGATGTT GCAGTCAGAGGATCACATCCAGATGGACACCTCCAGAGGGTCAAGTTCACGGTCAAGGGC TGAAATGCAGACACTCCCAGACCTGT
Restriction Sites	NotI-NotI
ACCN	AF070587
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF070587.1
RefSeq Size	1660 bp
RefSeq ORF	1660 bp
Locus ID	440193
Gene Summary	This gene encodes a ubiquitously expressed coiled-coil domain-containing protein that interacts with the dishevelled protein and is a negative regulator of the Wnt signalling pathway. The protein encoded by this gene has a PDZ-domain binding motif in its C-terminus with which it interacts with the dishevelled protein. Dishevelled is a scaffold protein involved in the regulation of the Wnt signaling pathway. The Wnt signaling pathway plays an important role in embryonic development, tissue maintenance, and cancer progression. Mutations in this gene cause autosomal recessive, primary non-syndromic congenital hydrocephalus; a condition characterized by excessive accumulation of cerebrospinal fluid in the ventricles of the brain. [provided by RefSeq, Jan 2013]

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