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LCLAT1 (NM_182551) Human Untagged Clone
CAT＃: SC107411
LCLAT1 (untagged)-Human lysocardiolipin acyltransferase 1 (LCLAT1), transcript variant 1
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1AGPAT8; AGPAT8; ALCAT1; HSRG1849; LYCAT; UNQ1849 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_182551, the custom clone sequence may differ by one or more nucleotides
ATGCATTCCAGGGGAAGGGAAATTGTGGTGCTTCTGAACCCATGGTCAATTAACGAGGCAGTTTCTAGCT ACTGCACGTACTTCATAAAGCAGGACTCTAAAAGCTTTGGAATCATGGTGTCATGGAAAGGGATTTACTT TATACTGACTCTGTTTTGGGGAAGCTTTTTTGGAAGCATTTTCATGCTGAGTCCCTTTTTACCTTTGATG TTTGTAAACCCATCTTGGTATCGCTGGATCAACAACCGCCTTGTGGCAACATGGCTCACCCTACCTGTGG CATTATTGGAGACCATGTTTGGTGTAAAAGTGATTATAACTGGGGATGCATTTGTTCCTGGAGAAAGAAG TGTCATTATCATGAACCATCGGACAAGAATGGACTGGATGTTCCTGTGGAATTGCCTGATGCGATATAGC TACCTCAGATTGGAGAAAATTTGCCTCAAAGCGAGTCTCAAAGGTGTTCCTGGATTTGGTTGGGCCATGC AGGCTGCTGCCTATATCTTCATTCATAGGAAATGGAAGGATGACAAGAGCCATTTCGAAGACATGATTGA TTACTTTTGTGATATTCACGAACCACTTCAACTCCTCATATTCCCAGAAGGGACTGATCTCACAGAAAAC AGCAAGTCTCGAAGTAATGCATTTGCTGAAAAAAATGGACTTCAGAAATATGAATATGTTTTACATCCAA GAACTACAGGCTTTACTTTTGTGGTAGACCGTCTAAGAGAAGGTAAGAACCTTGATGCTGTCCATGATAT CACTGTGGCGTATCCTCACAACATTCCTCAATCAGAGAAGCACCTCCTCCAAGGAGACTTTCCCAGGGAA ATCCACTTTCACGTCCACCGGTATCCAATAGACACCCTCCCCACATCCAAGGAGGACCTTCAACTCTGGT GCCACAAACGGTGGGAAGAGAAAGAAGAGAGGCTGCGTTCCTTCTATCAAGGGGAGAAGAATTTTTATTT TACCGGACAGAGTGTCATTCCACCTTGCAAGTCTGAACTCAGGGTCCTTGTGGTCAAATTGCTCTCTATA CTGTATTGGACCCTGTTCAGCCCTGCAATGTGCCTACTCATATATTTGTACAGTCTTGTTAAGTGGTATT TTATAATCACCATTGTAATCTTTGTGCTGCAAGAGAGAATATTTGGTGGACTGGAGATCATAGAACTTGC ATGTTACCGACTTTTACACAAACAGCCACATTTAAATTCAAAGAAAAATGAGTAA >OriGene 5' read for NM_182551 unedited
AGGAAGGGGAGCTGCAGCCCGGGCTTTTCCTCCTGAGCCATGGACCTTCCCCGGGCCGCG ACCTCGGGCTGAAAAACAGAGTGGGTACTCTCTTCTGGGAAGCTGGCAACAAATGGATGA TGTGATATATGCATTCCAGGGGAAGGGAAATTGTGGTGCTTCTGAACCCATGGTCAATTA ACGAGGCAGTTTCTAGCTACTGCACGTACTTCATAAAGCAGGACTCTAAAAGCTTTGGAA TCATGGTGTCATGGAAAGGGATTTACTTTATACTGACTCTGTTTTGGGGAAGCTTTTTTG GAAGCATTTTCATGCTGAGTCCCTTTTTACCTTTGATGTTTGTAAACCCATCTTGGTATC GCTGGATCAACAACCGCCTTGTGGCAACATGGCTCACCCTACCTGTGGCATTATTGGAGA CCATGTTTGGTGTAAAAGTGATTATAACTGGGGATGCATTTGTTCCTGGAGAAAGAAGTG TCATTATCATGAACCATCGGACAAGAATGGCCTGGGATNGTTCCTTNGTGGAATTGCCTG ATGCGATATAGCTACCTCAGATTGGAGAAAATTTGCCTCANAGCAGTTCAAAAAGGTGTT CCTGGATTTTGGGTTGGGCCATGCAGGCTGCTGCCTATATCTTCATTCTANGAANAGGGN AGGGATGACAGAGCCATTTCGAGACATGATGATAACTTTTTGTGATATTACGAACACTTC AACTCTATATTCCAGAAGGGACTGATCTACAGAAACAGAGNNTCTCGAAGTATGCATTGC TGAAAAAATGACTCGAATANTAA >OriGene 3' read for NM_182551 unedited
NCCAAGACTTGNAACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTAAAACTATTTAATTCACTCCTTTATTCTGGGATGTATATTACAGATAAC ACAACTCACAAATATACCATCAGACATTGAAAACTAAGGCCATTCTGTGAGTTATTTTTA AAACTTGGTGTTTTGCACATAATGATCTTAAAAAAAAATGAATTACCAAAACCAAGATTC TCTTCTAAAATGAAAATTTAATGCAGGTACAGGATAACTTTAGGGCTATATCTAATCTGA AGCTTATCAGGTAGCAAAACCATTTTCGTTTTCTACAGCATAAATAACAGCTCTAAGGCA ACCACTACCTCAGCATGAAGCTCATTTCTCCACGTTAGAGTAGTGTTTACCTGCTACAGT GACCAGTGTTTAAAGACCATTTCCCTTTCAGTAGCAAAAAGAGACTTTACCTAAGAAACA CACTACATACTACAGAATCCTTGGAACAAGAAACAGAAAGGGAGCTGTAACTAAGGCACT GAAAGCACATTATTTGTATAAAGAAATGTAAACAATTTAACACCAACAGGCTCCCTCCGT TGGAGTCTTTATTGTAAATGCACACAGGGGCATCATAGTCAGGGCTAGAGTTTGAAGTCT GGGTGAAACAAATGTTTCTGCAAAGTTCAAAGTCTTGCCTGCCAATATATTCTAACATGG TGCTGTTGGAATAACTATTTCAAAGTGACCCAGTGGAAAGCTGGGATTCCCTTGAATCCG AGTTTTAATAGCCTTACCAAACAGGAAGAAATTTTAGAGAAGTCCAAAATTTTGGGGCTC TTTCAAACATTAATTTGATTCCTTTATAAGTTCCTTCAAGGGATTTTAGGATAATTTGGG CTAATCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_182551 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182551.3, NP_872357.2 |
| RefSeq Size | 5077 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 253558 |
| UniProt ID | Q6UWP7 |
| Protein Families | Transmembrane |
| Protein Pathways | Ether lipid metabolism, Glycerolipid metabolism, Glycerophospholipid metabolism, Limonene and pinene degradation, Metabolic pathways, Phenylalanine metabolism, Tyrosine metabolism |
| Gene Summary | Exhibits acyl-CoA:lysocardiolipin acyltransferase (ALCAT) activity; catalyzes the reacylation of lyso-cardiolipin to cardiolipin (CL), a key step in CL remodeling (By similarity). Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors (By similarity). Also exhibits 1-acyl-sn-glycerol-3-phosphate acyltransferase activity (AGPAT) activity; converts 1-acyl-sn-glycerol-3- phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3- phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:16620771). Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities (PubMed:19075029). Required for establishment of the hematopoietic and endothelial lineages (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
LPS impairs oxygen utilization in epithelia by triggering degradation of the mitochondrial enzyme Alcat1
,Zou, C;Synan, MJ;Li, J;Xiong, S;Manni, M;Liu, Y;Chen, BB;Zhao, Y;Shiva, S;Tyurina, YY;Jiang, J;Lee, JS;Das, S;Ray, A;Ray, P;Kagan, VE;Mallampalli, RK;,
J. Cell. Sci.
,PubMed ID 26604221
[LCLAT1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220664 | LCLAT1 (Myc-DDK-tagged)-Human lysocardiolipin acyltransferase 1 (LCLAT1), transcript variant 1 |
CNY 5,488.00 |
|
| RC220664L1 | Lenti ORF clone of Human lysocardiolipin acyltransferase 1 (LCLAT1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC220664L2 | Lenti ORF clone of Human lysocardiolipin acyltransferase 1 (LCLAT1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC220664L3 | Lenti ORF clone of Human lysocardiolipin acyltransferase 1 (LCLAT1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220664L4 | Lenti ORF clone of Human lysocardiolipin acyltransferase 1 (LCLAT1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG220664 | LCLAT1 (tGFP-tagged) - Human lysocardiolipin acyltransferase 1 (LCLAT1), transcript variant 1 |
CNY 7,088.00 |
