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LC3B (MAP1LC3B) (NM_022818) Human Untagged Clone
CAT＃: SC108102
MAP1LC3B (untagged)-Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)
CNY 1,800.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108102 sequence for NM_022818 edited (data generated by NextGen Sequencing)
ATGCCGTCGGAGAAGACCTTCAAGCAGCGGCGCACCTTCGAACAAAGAGTAGAAGATGTC CGACTTATTCGAGAGCAGCATCCAACCAAAATCCCGGTGATAATAGAACGATACAAGGGT GAGAAGCAGCTTCCTGTTCTGGATAAAACAAAGTTCCTTGTACCTGACCATGTCAACATG AGTGAGCTCATCAAGATAATTAGAAGGCGCTTACAGCTCAATGCTAATCAGGCCTTCTTC CTGTTGGTGAACGGACACAGCATGGTCAGCGTCTCCACACCAATCTCAGAGGTGTATGAG AGTGAGAAAGATGAAGATGGATTCCTGTACATGGTCTGTGCCTCCCAGGAGACGTTCGGG ATGAAATTGTCAGTGTAA Clone variation with respect to NM_022818.4 30 c=>g;338 a=>g >OriGene 5' read for NM_022818 unedited
CAGAATTTTGTAATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGCAAGGCTC GCAGCCACCTCTCGGGAGTGCAGGGCCAGATCCTGTGCCAGCGGCCTTACACGGCCACAG TCGGATTCGCTGCCGCAGCAGCCGCCACCCCCAGGAGCCGCCGGGACCCTCGCGTCGTCG CCGCCGCCGCCCAGATCCCCACACCATGCCGTCGGAGAAGACCTTCAAGCAGCGGCGCAC CTTCGAACAAAGAGTAGAAGATGTCCGACTTATTCGAGAGCAGCATCCAACCAAAATCCC GGTGATAATAGAACGATACAAGGGTGAGAAGCAGCTTCCTGTTCTGGATAAAACAAAGTT CCTTGTACCTGACCATGTCAACATGAGTGAGCTCATCAAGATAATTAGAAGGCGCTTACA GCTCAATGCTAATCAGGCCTTCTTCCTGTTGGTGAACGGACACAGCATGGTCAGCGTCTC CACACCAATCTCAGAGGTGTATGAGAGTGAGAAAGATGAAGATGGATTCCTGTACATGGT CTGTGCCTCCCAGGAGACGTTCGGGATGAAATTGTCAGTGTAAAACCAGAAAAAATGCAT CTCTTCTAGAATTTTTTAAACCCTTACCAAGGAAAAAAAAAGGGATGTTACCAACTGAGA TCGATCAGTTCATCTAATCACAGATCATCAAACAGTAGTGTTCCCACCTAGGAGTGTTAG GAAAAGTGTGTTGTGTTTCAAGCAGAAAAACTGAGCTCNCAGTGAGCACATTCAGCTTTG GAAACTATATTATTTAATGTAGGCTAGCTTGGTTTCAAAATTTAAAGTTTAAAAATAAAT ACTTTGCATTCTANAAAAAAAAAAAAACTCGACTCTAGATGGCGGCCGCGTCATAGCTGG TTCTGGACAGATCCGGGTGGCATCCTGTGACCCTCCCAGTGCTCTCTGGCCTGGAGTGCC >OriGene 3' read for NM_022818 unedited
CTATGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAGAATGCAAAGT ATTTTATTTTTAAACTTTTAAAATTTGAAAACAAGCTAGCCTACATTAAATAATATAGTT TCCAAAGCTGAATGTGCTCACTTGGAGCTCAGTTTTTCTGCTTGAAACACAAACACAACT TCCTAACACTCCTAGGTGGGAACACTACTGTTTGATGATCTGTGATTAGATGAACTGATC GATCTCAGTTGGTAACATCCCTTTTTTTTTCCTTGGTAAGGGTTTAAAAAATTCTAGAAG AGATGCATTTTTTCTGGTTTTACACTGACAATTTCATCCCGAACGTCTCCTGGGAGGCAC AGACCATGTACAGGAATCCATCTTCATCTTTCTCACTCTCATACACCTCTGAGATTGGTG TGGAGACGCTGACCATGCTGTGTCCGTTCACCAACAGGAAGAAGGCCTGATTAGCATTGA GCTGTAAGCGCCTTCTAATTATCTTGATGAGCTCACTCATGTTGACATGGTCAGGTACAA GGAACTTTGTTTTATCCAGAACAGGAAGCTGCTTCTCACCCTTGTATCGTTCTATTATCA CCGGGATTTTGGTTGGATGCTGCTCTCGAATAAGTCGGACATCTTCTACTCTTTGTTCGA AGGTGCGCCGCTGCTTGAAGGTCTTCTCCGACGGCATGGTGTGGGAGATCTGGGCGGCGG CGGCGACGACGCGAGGGTCCCGGCGGCTCCTGNAGGTGGCGGCTGCTGCGGCAGCGAATC CGACTGTGGCCGTGTAAGGCCGCTGCACAGGATCTGGCCCTGCACTCCGAGAGTGGCTGC GAGCTTGCCTCGTGCGAATTCGGCGGCGCCTATAAGTGAGTCGTATACAAATNCTTGACG GTCACTAACGAGCCTGCTGAATAGACTNCCACGTACCGCTACGCCATTGCGAAGGGGGCG GGGGTATACACTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022818 |
| Insert Size | 930 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022818.3, NP_073729.1 |
| RefSeq Size | 2270 bp |
| RefSeq ORF | 378 bp |
| Locus ID | 81631 |
| UniProt ID | Q9GZQ8 |
| Domains | MAP1_LC3 |
| Gene Summary | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Activation of MTOR in pulmonary epithelium promotes LPS-induced acute lung injury
,Hu, Y;Lou, J;Mao, YY;Lai, TW;Liu, LY;Zhu, C;Zhang, C;Liu, J;Li, YY;Zhang, F;Li, W;Ying, SM;Chen, ZH;Shen, HH;,
Autophagy
,PubMed ID 27658023
[LC3B]
|
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Other Versions
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| RC207356L1 | Lenti ORF clone of Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), Myc-DDK-tagged |
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| RC207356L2 | Lenti ORF clone of Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), mGFP tagged |
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| RC207356L3 | Lenti ORF clone of Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), Myc-DDK-tagged |
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| RC207356L4 | Lenti ORF clone of Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), mGFP tagged |
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| RG207356 | MAP1LC3B (tGFP-tagged) - Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B) |
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| SC322139 | MAP1LC3B (untagged)-Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B) |
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