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LC3B (MAP1LC3B) (NM_022818) Human Untagged Clone
CAT＃: SC322139
MAP1LC3B (untagged)-Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)
CNY 1,800.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for SC322139
ACGGGCGAGCGGCGACCCTGCCTCCCGGAGACGGCGCGGCCTGCCCTGCGCGCCTCAGCC
CCGGGTGCCGGCGTCTCGGGCAGCACCACCAAGTCTCTCTGGAGGGGAAAGGATGGTCGG ATTTGCCCCATGTCCCTTTCTCTGACCCCTCCCTCAAGAGTGCCCCGGGACACCCCGCCT GTGGCTCAGCCTCCCCCGCCCCGCGCTGCCATCTCCTCAGGGCCGGGCAGCAGGCTCCCG AGCGCCCACAGACCCGGGGTGCGGCCCAGCCCACAACCGTCACCTCAGGGGCCTCAGGCG CCCAGCGGTGCTGGGCGGGGCTGGGGCACGACCGGGAGCATGCGCAGAGCGCGCGTTTCG CCCATCGCGCACGCGCACACACCTGCTCCGCCCCCACGCTGCGTGCCGCTGCTGGGTTCC GCCACGCCCGTCATGGCGGCGGCCCCGGCCGGCTCTGGCCCCGCCCCTCGGTGACGCGTC GCGAGTCACCTGACCAGGCTGCGGGCTGAGGAGATACAAGGGAAGTGGCTATCGCCAGAG TCGGATTCGCCGCCGCAGCAGCCGCCGCCCCCGGGAGCCGCCGGGACCCTCGCGTCGTCG CCGCCGCCGCCGCCCAGATCCCCGCACCATGCCGTCGGAGAAGACCTTCAAGCAGCGCCG CACCTTCGAACAAAGAGTAGAAGATGTCCGACTTATTCGAGAGCAGCATCCAACCAAAAT CCCGGTGATAATAGAACGATACAAGGGTGAGAAGCAGCTTCCTGTTCTGGATAAAACAAA GTTCCTTGTACCTGACCATGTCAACATGAGTGAGCTCATCAAGATAATTAGAAGGCGCTT ACAGCTCAATGCTAATCAGGCCTTCTTCCTGTTGGTGAACGGACACAGCATGGTCAGCGT CTCCACACCAATCTCAGAGGTGTATGAGAGTGAGAAAGATGAAGATGGATTCCTGTACAT GGTCTATGCCTCCCAGGAGACGTTCGGGATGAAATTGTCAGTGTAAAACCAGAAAAAATG CATCTCTTCTAGAATTGTTTAAACCCTTACCAAGGAAAAAAAAGGGGTGTTACCAACTGA GATCGATCAGTTCATCCAATCACAGATCATGAAACAGTAGTGTTCCCACCTAGGAGTGTT AGGAAGTTGTGTTTGTGTTTCAAGCAGAAAAACTGAGCTCCAAGTGAGCACATTCAGCTT TGGAAACTATATTATTTAATGTAGGCTAGCTTGTTTTCAAATTTTAAAAGTTTAAAAATA AAATACTTTGCATTCTAAGTTGCCAATAAAATAGACCTTCAAGTTATTTTAATGCTCTTT TCTCACTAATAGGAACTTGTAATTCCAGCAGTAATTTAAAGGCTTTCAGAGAGACCCTGA GTCTTCTCTTCAGGTTCACAGAACCCGCCGCCTTTTTGGGTAGAAGTTTTCTACTCAGCT AGAGAGATCTCCCTAAGAGGATCTTTAGGCCTGAGTTGTGAAGCGCAACCCCCGCAAAAC GCATTTGCCATCACAGTTGGCACAAACGCAGGGTAAACGGGCTGTGTGAGAAAACGGCCC TGACTGTAAACTGCTGAAGGTCCCTGACTCCTAAGAGAACCACACCCAAAGTCCTCACTC TTGCAGGGGTAGACATTTCTGGTTTGGTTTGTTCTCTAGATAGTTACACACATAAAGACA CCACTCAAAAGGAAACTTGAATAATTTATAATTTTGATCGAGTTTCTTAAAAGACCCTGG AGAAAGAGTGGCATTTCTTCTGTTTCAGGTTTTGTCTGAGTTCAAACTAGTGCCTGTGTT GTTACGGAAAGCAGCAGTGTACCAGTGTCACTCTGGAGTACAGCGGGAGAAACACAAAAT AGTATAACTGAAAACATTAACATTCAGACACACTCCCTTCTGCCTTCCGGCTTAAAGCTG TGGATGATCCACGTTTTTGTTTTTTTAATGTTAAATGTGTAACTCAGTATTACTGAAAAG GTACCCACATTTTGAATAGTAGTTATCACTCTTAGGTCAGACAGCCATCAGAATTCTCCC ACACCAAGTGCATGTCAGTTGTGGAGAAAACATAGCAAAAAGAGCCGTACGCTCTTTACA GATACTAATGTCAAGAGTTAAACCTCCTCAGGTTCAACCTGTGATAAAAGACTAGTGCTT CCCAGTACTTGCATGGGGTTCACTATTTATAGTTTTCTTGGGAGTATCACAGGAAAATCA CAATTACACCACTTTAGACCCTATGTGTAGCAGGTCACAACTTACCCTTGTGTGTTTAGA TGTGTATGAAATACCTGTATACGTTAGTGAAAGCTGTTTACTGTAACGGGGAAAACCAGA TTCTTTGCATCTGGGCCCTCTACTGATTGTTAAAGGAGTTCCTGTCACCTGCTCCCCCCA CCCCCGCATGCGTCTGTCCACTTGGCTAACTTTTAATATGTGTATTTTTACATTATGTAT ATTCTTAACTGGACTGTCTCGTTTAGACTGTATACATCATATCTGACATTATTGTAACTA CCGTGTGATCAGTAAGATTCCTGTAAGAAATACTGCTTTTTAAGAAAAAAAATAACATGC TGAGGGGTGACCTATATCCCATGTGAGTGGTCACTTTATTTATAGGATCTTTAAAACATT TTTAATGAACTAAGTTGAATAAAGGCACAATTAAAAACTGTCATCAAAAAAAAAAAAAAA A |
| Restriction Sites | Please inquire |
| ACCN | NM_022818 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022818.3, NP_073729.1 |
| RefSeq Size | 2270 bp |
| RefSeq ORF | 378 bp |
| Locus ID | 81631 |
| UniProt ID | Q9GZQ8 |
| Domains | MAP1_LC3 |
| Gene Summary | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Autophagy protein microtubule-associated protein 1 light chain-3B (LC3B) activates extrinsic apoptosis during cigarette smoke-induced emphysema
,Zhi-Hua Chen, Hilaire C. Lam, Yang Jin, Hong-Pyo Kim, Jiaofei Cao, Seon-Jin Lee, Emeka Ifedigbo, Harikrishnan Parameswaran, Stefan W. Ryter, and Augustine M. K. Choi,
PNAS, Nov 2010; 107: 18880 - 18885
[MAP1LC3B]
|
|
Autophagic Protein LC3B Confers Resistance Against Hypoxia-Induced Pulmonary Hypertension
,Seon-Jin Lee, Akaya Smith, Lanping Guo, Tero-Pekka Alastalo, Molong Li, Hirofumi Sawada, Xiaoli Liu, Zhi-Hua Chen, Emeka Ifedigbo, Yang Jin, Carol Feghali-Bostwick, Stefan W Ryter, Hong Pyo Kim, Marlene Rabinovitch, and Augustine MK Choi,
Am. J. Respir. Crit. Care Med., Oct 2010; 10.1164/rccm.201005-0746OC
[MAP1LC3B]
|
|
Celecoxib-induced apoptosis is enhanced by ABT-737 and by inhibition of autophagy in human colorectal cancer cells
,null,
Autophagy
,PubMed ID 20104024
[MAP1LC3B]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207356 | MAP1LC3B (Myc-DDK-tagged)-Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B) |
CNY 1,800.00 |
|
| RC207356L1 | Lenti ORF clone of Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC207356L2 | Lenti ORF clone of Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), mGFP tagged |
CNY 4,200.00 |
|
| RC207356L3 | Lenti ORF clone of Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC207356L4 | Lenti ORF clone of Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), mGFP tagged |
CNY 4,200.00 |
|
| RG207356 | MAP1LC3B (tGFP-tagged) - Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B) |
CNY 3,400.00 |
|
| SC108102 | MAP1LC3B (untagged)-Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B) |
CNY 1,800.00 |
