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PXR (NR1I2) (NM_003889) Human Untagged Clone
CAT＃: SC109997
NR1I2 (untagged)-Human nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1
CNY 3,656.00
| Cited in 17 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BXR; ONR1; PAR; PAR1; PAR2; PARq; PRR; PXR; SAR; SXR |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003889, the custom clone sequence may differ by one or more nucleotides
CTGGAGGTGAGACCCAAAGAAAGCTGGAACCATGCTGACTTTGTACACTGTGAGGACACAGAGTCTGTTC CTGGAAAGCCCAGTGTCAACGCAGATGAGGAAGTCGGAGGTCCCCAAATCTGCCGTGTATGTGGGGACAA GGCCACTGGCTATCACTTCAATGTCATGACATGTGAAGGATGCAAGGGCTTTTTCAGGAGGGCCATGAAA CGCAACGCCCGGCTGAGGTGCCCCTTCCGGAAGGGCGCCTGCGAGATCACCCGGAAGACCCGGCGACAGT GCCAGGCCTGCCGCCTGCGCAAGTGCCTGGAGAGCGGCATGAAGAAGGAGATGATCATGTCCGACGAGGC CGTGGAGGAGAGGCGGGCCTTGATCAAGCGGAAGAAAAGTGAACGGACAGGGACTCAGCCACTGGGAGTG CAGGGGCTGACAGAGGAGCAGCGGATGATGATCAGGGAGCTGATGGACGCTCAGATGAAAACCTTTGACA CTACCTTCTCCCATTTCAAGAATTTCCGGCTGCCAGGGGTGCTTAGCAGTGGCTGCGAGTTGCCAGAGTC TCTGCAGGCCCCATCGAGGGAAGAAGCTGCCAAGTGGAGCCAGGTCCGGAAAGATCTGTGCTCTTTGAAG GTCTCTCTGCAGCTGCGGGGGGAGGATGGCAGTGTCTGGAACTACAAACCCCCAGCCGACAGTGGCGGGA AAGAGATCTTCTCCCTGCTGCCCCACATGGCTGACATGTCAACCTACATGTTCAAAGGCATCATCAGCTT TGCCAAAGTCATCTCCTACTTCAGGGACTTGCCCATCGAGGACCAGATCTCCCTGCTGAAGGGGGCCGCT TTCGAGCTGTGTCAACTGAGATTCAACACAGTGTTCAACGCGGAGACTGGAACCTGGGAGTGTGGCCGGC TGTCCTACTGCTTGGAAGACACTGCAGGTGGCTTCCAGCAACTTCTACTGGAGCCCATGCTGAAATTCCA CTACATGCTGAAGAAGCTGCAGCTGCATGAGGAGGAGTATGTGCTGATGCAGGCCATCTCCCTCTTCTCC CCAGACCGCCCAGGTGTGCTGCAGCACCGCGTGGTGGACCAGCTGCAGGAGCAATTCGCCATTACTCTGA AGTCCTACATTGAATGCAATCGGCCCCAGCCTGCTCATAGGTTCTTGTTCCTGAAGATCATGGCTATGCT CACCGAGCTCCGCAGCATCAATGCTCAGCACACCCAGCGGCTGCTGCGCATCCAGGACATACACCCCTTT GCTACGCCCCTCATGCAGGAGTTGTTCGGCATCACAGGTAGCTGA >OriGene 5' read for NM_003889 unedited
CCCGTTAGGAATTGTAAACGACTCATATAGGCGGCCTGCGAATTCGCACCAGCCTATGAC TAGGACGGGAAGAGGAAGCACTGCCTTTACTTCAGTGGGAATCTCGGCCTCAGCCTGCAA GCCAAGTGTTCACAGTGAGAAAAGCAAGAGAATAAGCTAATACTCCTGTCCTGAAAAAGG CAGCGGCTCCTTGGTAAAGCTACTCCTTGATCGATCCTTTGCACCGGATTGTTCAAAGTG GACCCCAGGGGAGAAGTCGGAGCAAAGAACTTACCACCAAGCAGTCCAAGAGGCCCAGAA GCAAACCTGGAGGTGAGACCCAAAGAAAGCTGGAACCATGCTGACTTTGTACACTGTGAG GACACAGAGTCTGTTCCTGGAAAGCCCAGTGTCAACGCAGATGAGGAAGTCGGAGGTCCC CAAATCTGCCGTGTATGTGGGGACAAGGCCACTGGCTATCACTTCAATGTCATGACATGT GAAGGATGCAAGGGCTTTTTCAGGAGGGCCATGAAACGCAACGCCCGGCTGAGGTGCCCC TTCCGGAAGGGCGCCTGCGAGATCACCCGGAAGACCCGGCGACAGTGCCAGGCCTGCCGC CTGCGCAAGTGCCTGGAGAGCGGCATGAAGAAGGAGATGATCATGTCCGACGAGGCCGTG GAGGAGAGGCGGGCCTTGATCAAGCGGAAGAGAAGTGAACGGACAGGGACTCAGCCACTG NGAGTGCAGGGGCTGACAGAGGAGCAGCTGATGATGATCAGGGAGCTGATGGACGCTCAG ATGAAAACCTTTGACACTACCTTCTCCCATTTCAAGAATTTCCTGCTGCCAGGGGTGCTT AGCAGTGGCTGCGAGTTGCCAGAGTCTCTGCAGGCCCCATCTGAGGAAAGAAGCTGCCTA GTGTAGCCAGGTCTGGAAAGATCTGTGCTCTTTGAAGGTCT >OriGene 3' read for NM_003889 unedited
CCCCCCCATTAGTGTAGNCNCGCGGNCCGCTATTCTAGACNCGACTTTTTTTTTTTTTTT TTTTTAGCCAAAGTACATTATTTAATTCCTTTTGCCTTGATTTGACATTAAACCCAGGGC TACATTTCCCAAAACTAGTTCTTAGGTGTGGAATGCCTTATATAAAGTATAATTTGTGAA AATGCAAAAAAATTTTTACTCACAAGTGGCTATAAACAAGGCAGGCACTTTCATACCCTT TAGCTTTGAGAATAGATGTGTACACAGAAATATAAGGTGCATGTAAATGGTTCTTCTCCG GTGTGTTTAACTGATTGTCAGCGTAGCCTTGTCACAGAGCATACCCAGCATTCCAGCCCC AGGAACAAACCCAGGCCCCATCAAGTGGCAAACAGACCTACCTACACCAAATCACACACA CGCACTCACAGCTGCAGTTTGTGCCCTTGCTCCTACAGACTCATGTCCTCGGGAACCTTC ACTTGGGTACCCAGTGCCCGCCATCACAGCTCAGCATCCACACAGGGAAATTCCTTGTGC TGCTTTTGCCACTTATCATTTCTCCTTTTGATCCACAACGTTTGTGTTTCTGTTTGTTTT TTTAACTATAAACTCTGTGAGCTCAGGATCTATATCTGAGTCAATGCTGTATACCCCACA GCAGGCTACCTATTATCGGTGCTTAATAATGGGTTCAAATGGATGCAGAGACCAGAATGA CGTGGCCGGAATGAGGCCATGCCCAGCTTCTGACATTTGGTGCCGGCACTCCTGGCTTCT CATCTTTCTAGAGGACGTCCCACCGAACTTATGCCCCTGCCGATGAGTACAGGCCTGGAG CCCCAAAGCAGTTGGAAGAGAGGGAAACCAATGGAACATGGAGACGCACTATTACAGTTA GCATGAAAAAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003889 |
| Insert Size | 1305 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003889.2, NP_003880.3 |
| RefSeq Size | 4446 bp |
| RefSeq ORF | 1305 bp |
| Locus ID | 8856 |
| UniProt ID | O75469 |
| Domains | HOLI, zf-C4 |
| Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
| Gene Summary | This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) contains an alternate 5' terminal exon compared to transcript variant 2, and initiates translation from an in-frame, downstream non-AUG (CUG) codon, resulting in a shorter isoform (1) with a different N-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Citations (17)
| The use of this cDNA Clones has been cited in the following citations: |
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Time-Resolved Effect of Interferon-Alpha 2a on Activities of Nuclear Factor Kappa B, Pregnane X Receptor and on Drug Disposition Genes
,Theile, D;Wagner, L;Bay, C;Haefeli, WE;Weiss, J;,
Pharmaceutics
,PubMed ID 34071580
[NR1I2]
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In vitro evidence suggesting that the toll-like receptor 7 and 8 agonist resiquimod (R-848) unlikely affects drug levels of co-administered compounds
,Theile, D;Wagner, L;Haefeli, W;Weiss, J;,
European Journal of Pharmaceutical Sciences
,PubMed ID 33813039
[NR1I2]
|
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Nobiletin, sinensetin, and tangeretin are the main perpetrators in clementines provoking food-drug interactions in vitro
,Weiss, J;Gattuso, G;Barreca, D;Haefeli, WE;,
Food Chem
,PubMed ID 32187570
[NR1I2]
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The aglycone diosmetin has the higher perpetrator drug-drug interaction potential compared to the parent flavone diosmin
,Bajraktari, G;Weiss, J;,
Journal of Functional Foods
[NR1I2]
|
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Patchouli alcohol activates PXR and suppresses the NF-κB-mediated intestinal inflammatory
,Zhang, G;Liu, M;Song, M;Wang, J;Cai, J;Lin, C;Li, Y;Jin, X;Shen, C;Chen, Z;Cai, D;Zhu, C;Lin, C;Liu, C;,
J Ethnopharmacol
,PubMed ID 31614203
[NR1I2]
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Activating the pregnane X receptor by imperatorin attenuates dextran sulphate sodium-induced colitis in mice
,Liu, M;Zhang, G;Zheng, C;Song, M;Liu, F;Huang, X;Bai, S;Huang, X;Lin, C;Zhu, C;Hu, Y;Mi, S;Liu, C;,
Br. J. Pharmacol.
,PubMed ID 29945292
[NR1I2]
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Probing ligand structure-activity relationships in pregnane X receptor (PXR): efavirenz and 8-hydroxyefavirenz exhibit divergence in activation
,Narayanan, B;Lade, J;Heck, CJS;Dietz, KD;Wade, HV;Bumpus, NN;,
ChemMedChem
,PubMed ID 29430850
[NR1I2]
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Differential activation of pregnane X receptor by carnosic acid, carnosol, ursolic acid, and rosmarinic acid
,Seow, CL;Lau, AJ;,
Pharmacol. Res
,PubMed ID 28288941
[NR1I2]
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Clementine juice has the potential for drug interactions - In vitro comparison with grapefruit and mandarin juice
,Theile, D;Hohmann, N;Kiemel, D;Gattuso, G;Barreca, D;Mikus, G;Haefeli, WE;Schwenger, V;Weiss, J;,
Eur J Pharm Sci
,PubMed ID 27890698
[NR1I2]
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The long chain a-tocopherol metabolite a-13'-COOH and ?-tocotrienol induce P-glycoprotein expression and activity by activation of the pregnane X receptor in the intestinal cell line LS 180
,Podszun, MC;Jakobi, M;Birringer, M;Weiss, J;Frank, J;,
Molecular Nutrition & Food Research
,PubMed ID 27714977
[NR1I2]
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Bortezomib and ixazomib protect firefly luciferase from degradation and can flaw respective reporter gene assays
,Becker, JP;Clemens, JR;Theile, D;Weiss, J;,
Anal. Biochem.
,PubMed ID 27325500
[NR1I2]
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Cross-talk between EPAS-1/HIF-2a and PXR signaling pathway regulates multi-drug resistance of stomach cancer cell
,Zhao, J;Bai, Z;Feng, F;Song, E;Du, F;Zhao, J;Shen, G;Ji, F;Li, G;Ma, X;Hang, X;Xu, B;,
Int. J. Biochem. Cell Biol.
,PubMed ID 26783937
[NR1I2]
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Obatoclax as a perpetrator in drug-drug interactions and its efficacy in multidrug resistance cell lines
,Theile, D;Allendorf, D;Köhler, BC;Jassowicz, A;Weiss, J;,
J. Pharm. Pharmacol.
,PubMed ID 26255619
[NR1I2]
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3-Hydroxyflavone and structural analogues differentially activate pregnane X receptor: Implication for inflammatory bowel disease
,Lau, AJ;Chang, TK;,
Pharmacol. Res.
,PubMed ID 26238175
[NR1I2]
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Desmethyl bosentan displays a similar in vitro interaction profile as bosentan
,Weiss, J;Baumann, S;Theile, D;Haefeli, WE;,
Pulm Pharmacol Ther
,PubMed ID 25535031
[NR1I2]
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Pharmacokinetic interaction profile of riociguat, a new soluble guanylate cyclase stimulator, in vitro
,Rickert, V;Haefeli, WE;Weiss, J;,
Pulm Pharmacol Ther
,PubMed ID 24657506
[NR1I2]
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Induced CYP3A4 Expression in Confluent Huh7 Hepatoma Cells as a Result of Decreased Cell Proliferation and Subsequent Pregnane X Receptor Activation
,Louise Sivertsson, Irene Edebert, Margareta Porsmyr Palmertz, Magnus Ingelman-Sundberg, and Etienne P. A. Neve,
Mol. Pharmacol., Mar 2013; 83: 659 - 670.
[NR1I2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205691 | NR1I2 (Myc-DDK-tagged)-Human nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1 |
CNY 5,488.00 |
|
| RC205691L1 | Lenti ORF clone of Human nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205691L2 | Lenti ORF clone of Human nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC205691L3 | Lenti ORF clone of Human nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205691L4 | Lenti ORF clone of Human nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG205691 | NR1I2 (tGFP-tagged) - Human nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1 |
CNY 7,088.00 |
