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ASS1 (NM_000050) Human Untagged Clone
CAT＃: SC110830
ASS1 (untagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 1
CNY 3,656.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASS; CTLN1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110830 sequence for NM_000050 edited (data generated by NextGen Sequencing)
ATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGACACCTCGTGCATC CTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGCCAACATTGGCCAG AAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGCCAAAAAGGTGTTC ATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGCCATCCAGTCCAGC GCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCCCTGCATCGCCCGC AAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCACGGCGCCACAGGA AAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACTGGCCCCCCAGATAAAG GTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAAGGGCCGCAATGACCTG ATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCCCAAGAACCCGTGGAGC ATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCTGGAGAACCCCAAGAAC CAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAAAGCCCCCAACACCCCT GACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGAAGGTGACCAACGTCAAGGAT GGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAACGAAGTCGCGGGCAAG CATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT ATCTACGAGACCCCAGCAGGCACCATCCTTTACCATGCTCATTTAGACATCGAGGCCTTC ACCATGGACCGGGAAGTGCGCAAAATCAAACAAGGCCTGGGCTTGAAATTTGCTGAGCTG GTGTATACCGGTTTCTGGCACAGCCCTGAGTGTGAATTTGTCCGCCACTGCATCGCCAAG TCCCAGGAGCGAGTGGAAGGGAAAGTGCAGGTGTCCGTCCTCAAGGGCCAGGTGTACATC CTCGGCCGGGAGTCCCCACTGTCTCTCTACAATGAGGAGCTGGTGAGCATGAACGTGCAG GGTGATTATGAGCCAACTGATGCCACCGGGTTCATCAACATCAATTCCCTCAGGCTGAAG GAATATCATCGTCTCCAGAGCAAGGTCACTGCCAAATAG Clone variation with respect to NM_000050.4 >OriGene 5' read for NM_000050 unedited
GATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCCACCGCTGCC CGAGCCCGACGCTATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGA CACCTCGTGCATCCTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGC CAACATTGGCCAGAAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGC CAAAAAGGTGTTCATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGC CATCCAGTCCAGCGCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCC CTGCATCGCCCGCAAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCA CGGCGCCACAGGAAAGGGGAACGATCAGGTCCCGGTTTGAGCTCAGCTGCTACTCACTGG CCCCCCAGATAAAGGTCATTGCTCNTTGGAGGATGCCTGAATTCTACAACCGGTTCAAGG GCCGCAATGACCTGATGGAGTACGCAAAGCAACACGGGATTCCAATCCCGGTCACTCCCA AGACCCGTGGAGATGGGNATGAGAACCTCATGCACATCAGCTACGAAGCTGGGAATCCTG GAGAACCCCAAGAACCAAGCGCCTNNCAGGTCTCTACACCGAAGACCCAGGACCCAGCCA AAGCCCCCAACACCCCTGAATTNTTCGAGAAAACGAGTTCAAANAAAGGGGGTCTGTGGG AAAGGTGAACAACGTCAAAGGAATGGGACCACCCACCAGACCTCCTTGGAGCTCCTCATG TACCTGAACCAAANTCGCGGCAGCATGGGCGGGGGGCCGAATGCAATTCGGGGGAGACCC GCTCATTGGAAAGAACCCCCCGAGGTTTTAAAAAACCCCCACAGCCACAATCCTTCCATG CTCATTAAAAAACGAGGCCCTACATGACCGCGGAAA >OriGene 3' read for NM_000050 unedited
NGGTTTTTACTNATGNANCGCGGNNCCGCATTTNANGAATCGATTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGGGCATTTTTAAAGCTCCCCACC GAAGTTGCCCCCCACCCTTCCTTTTAATAACAACGTTTGCAGGCTTAAGGGGGACCAGGG AACAAAGCTGGGGCCTGGAAGCCCCATTACGCTGCCAGCCGGGGAAAACAAGTCACAATT ACAAATTATCACAACAATTACCGCCTGTACTTGGGGGATCTGCAAATTGAGGAGGCCCCA CCTCCTTATTGTACACGGGTTTATTTGGCAGGGACCTTGCTTTGGAAACAAAAATTTTCC TTCACCCTGAGGGAATTGATGTTGATAAACCCGGGGGCATAAGTTGGCTAAAAATAACCC TGCACGTTAATGCTCACCAACTCCTTATTGTAAAAAAACAGGGGGGACTCCCGGCCGAGG ATGTACACCTGGCCCTTGAGGACGGACACCTGCACTTTCCCTTCCACTCGCTCCTGGGAC TTGGCAATGCAGGGGCGGACAAATTCACACTCAGGGCTGGGCCAAAAACCGGTATACACC AGCTCAAAAAATTTCAAGCCCAGGCCTTGTTTGATTTTGCGCACTTCCCGGCCCATGGGG AAGGCCTCAATGTCTAAATGACCATGGAAAAGGATGGGGCCTGCTGGGGTCTCGAAAAAC CTCGGGACTAATTCCAATGAAGCGGTTCTCCACAATGTAAATACGGCCCACGCCATGCTT GCCCGGAACTTNGTTAAGGTACATGAAAAGCTCCAAGGAGGTCTGGGGGGGTGGGGGCAT CCTTGACGTTGGACACCCTCACAGGACCCCTTTTTTGAACTCGATCTCAAATGTCAAGGG GTGTTGGGGCTTTGGCTGGGCCCTGGGGCCTTCGGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000050 |
| Insert Size | 1600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000050.3, NP_000041.2 |
| RefSeq Size | 1582 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 445 |
| UniProt ID | P00966 |
| Domains | Arginosuc_synth |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways |
| Gene Summary | The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (1) represents the longer variant. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Argininosuccinate Synthase 1-Deficiency Enhances the Cell Sensitivity to Arginine through Decreased DEPTOR Expression in Endometrial Cancer
,Ohshima, K;Nojima, S;Tahara, S;Kurashige, M;Hori, Y;Hagiwara, K;Okuzaki, D;Oki, S;Wada, N;Ikeda, JI;Kanai, Y;Morii, E;,
Sci Rep
,PubMed ID 28358054
[ASS1]
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Nutritional Stress and Arginine Auxotrophy Confer High Sensitivity to Chloroquine Toxicity in Mesothelioma Cells
,Sonia Battisti, Davide Valente, Loredana Albonici, Roberto Bei, Andrea Modesti, and Camilla Palumbo,
Am. J. Respir. Cell Mol. Biol., Apr 2012; 46: 498 - 506.
[ASS1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223189 | ASS1 (Myc-DDK-tagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 1 |
CNY 3,656.00 |
|
| RC223189L1 | Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC223189L2 | Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC223189L3 | Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223189L4 | Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG223189 | ASS1 (tGFP-tagged) - Human argininosuccinate synthase 1 (ASS1), transcript variant 1 |
CNY 5,256.00 |
