TFIIB (GTF2B) (NM_001514) Human Untagged Clone
CAT#: SC110914
GTF2B (untagged)-Human general transcription factor IIB (GTF2B)
CNY 2,400.00
CNY 2,950.00
Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | TF2B; TFIIB |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_001514, the custom clone sequence may differ by one or more nucleotides
ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAG TGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGA TGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTG ACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGC ATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTC TCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTC TAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA ACTGGGGACTTCATGTCCAGGTTCTGTTCCAACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACAC ATATAGCCCGTAAAGCTGTGGAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGC TATTTACATGGCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTTCCTACAGACT TCAAATTTGACACCCCAGTGGACAAACTACCACAGCTATAA >OriGene 5' read for NM_001514 unedited
CGTTTACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCTGTTGTGT CTTGTTGCGGGCACCGCAGTCGCCGTGAAGATGGCGTCTACCAGCCGTTTGGATGCTCTT CCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAGTGGAGGACTACAGAGCCGGT GATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGATGTGGGATCT GAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCT GCAAGTTTTGACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCT GATCGGGCAATGATGAATGCATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTA CCTCGAAATATAGTTGATCGAACAAATAATTTATTCAAGCAAGTATATGAACAGAAGAGC CTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTCTCTATATTGCCTGTAGACAA GAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTCTAAGAAAGAA ATTGGTCGGTGGTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA ACTGGGGACTTCATGTCCAGGTTCTGGTCCAACCTTTGTCTTCCTNNAACAGTACAGATG GCAGCTACACATATAGCCCCGTAAGCTGTGGAATTGGACTTGGTTCCTGGGNNAGAGCCC CATCTCTGTGGCAGCGGCAGCTATTTACATGGCCTCACAGCCATCAGCTGGAAAGAGGAC CCACAAGAN >OriGene 3' read for NM_001514 unedited
TGTACCGCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTTTTGAAAAA TGTTTTATTCAAGATTACATAATTTCAAATATCTTCCAAAATACAGTTTCCTAGATTGCT ACAAAAGAAATTTGATAAGAAATTTAAATCATTAAATATGTTTCACTAGTATATACATAC AGAATGCCAAGCAATAGTATTCAGCCCTGGAATGCGTACCATGTCTTTTGTTTTTTCTCA TGAAAGGCTCAACCCAGCATTTTGCATAGGCTATGTACAACAGGCAAAGTTTTGTATTCA AGAATTTGACGTACGCTGCCTCAATTTATAGCCTGTGGCACTTCGCCCACTGGGGTGTCA CATTTGAAGCCTGTAGGAACCAGATTTGGGGCTCGAGGATAGACCAGTCTATAGGACTGT CTGATTGTACCATCAGCAACACCAGCATTCTCTCCAATTTCCTTCTGCGTCCTCTCTTAC CTGATGCCCGCGAGGCCATGCCAATAGCTGTCGTTGCCACAGACACGGCCGCTCCTCCTT ATGAGTCCCGTCCCCCTCACAGCCTTACGGGCTACATGTGTAGCTGCCATCCGCCCACGG CTAGGAAGACAAAGCGTCGCCACCGAACCTGGACACGAATTCCCTCAGTTGTCATTCACA CCCACATTGGTTCCTAGCGCCTTTCAACTCAGTTCCAACCACCGCCCCCCCCCTCTCTAT CAACCCGGCCCCGGCACATATTTTTTTCACCGCCCTAAGACCCCCCCCCCTGCCTTAAGC CATATACTATTCTTTTCCCTCCTTTTTCCCCACCACCCCCTCCCCTCCAGGCTTCTCTTG GAACCAAACTTGTTTCGCACCCATATCCGTTCGCCAACCTCTCTCGCGACGGTTCATGCT CTCCCCCGCCCGGCAGGTGACTATTTGAAGCACTTATCACTCGCCACAGCACCGCCCCGT TTTCACACCGCCCTCTAACTGCCCTCCCGTAACCTGGGCCCCGCTTTTCCT |
Restriction Sites | NotI-NotI |
ACCN | NM_001514 |
Insert Size | 1350 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001514.3, NP_001505.1 |
RefSeq Size | 1304 bp |
RefSeq ORF | 951 bp |
Locus ID | 2959 |
UniProt ID | Q00403 |
Domains | transcript_fac2, CYCLIN |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Basal transcription factors |
Gene Summary | This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Acute Targeting of General Transcription Factor IIB Restricts Cardiac Hypertrophy via Selective Inhibition of Gene Transcription
,Sayed, D;Yang, Z;He, M;Pfleger, JM;Abdellatif, M;,
Circ Heart Fail
,PubMed ID 25398966
[GTF2B]
|
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204607 | GTF2B (Myc-DDK-tagged)-Human general transcription factor IIB (GTF2B) |
CNY 2,400.00 |
|
RC204607L3 | Lenti ORF clone of Human general transcription factor IIB (GTF2B), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204607L4 | Lenti ORF clone of Human general transcription factor IIB (GTF2B), mGFP tagged |
CNY 5,890.00 |
|
RG204607 | GTF2B (tGFP-tagged) - Human general transcription factor IIB (GTF2B) |
CNY 4,000.00 |
|
SC321111 | GTF2B (untagged)-Human general transcription factor IIB (GTF2B) |
CNY 2,400.00 |