TFIIB (GTF2B) (NM_001514) Human Untagged Clone

CAT#: SC110914

GTF2B (untagged)-Human general transcription factor IIB (GTF2B)



  "NM_001514" in other vectors (5)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


GTF2B mouse monoclonal antibody, clone OTI2A1 (formerly 2A1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms TF2B; TFIIB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001514, the custom clone sequence may differ by one or more nucleotides


ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAG
TGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGA
TGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT
CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTG
ACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGC
ATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT
TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTC
TCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTC
TAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA
ACTGGGGACTTCATGTCCAGGTTCTGTTCCAACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACAC
ATATAGCCCGTAAAGCTGTGGAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGC
TATTTACATGGCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT
GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTTCCTACAGACT
TCAAATTTGACACCCCAGTGGACAAACTACCACAGCTATAA


>OriGene 5' read for NM_001514 unedited
CGTTTACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCTGTTGTGT
CTTGTTGCGGGCACCGCAGTCGCCGTGAAGATGGCGTCTACCAGCCGTTTGGATGCTCTT
CCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAGTGGAGGACTACAGAGCCGGT
GATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGATGTGGGATCT
GAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT
CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCT
GCAAGTTTTGACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCT
GATCGGGCAATGATGAATGCATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTA
CCTCGAAATATAGTTGATCGAACAAATAATTTATTCAAGCAAGTATATGAACAGAAGAGC
CTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTCTCTATATTGCCTGTAGACAA
GAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTCTAAGAAAGAA
ATTGGTCGGTGGTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA
ACTGGGGACTTCATGTCCAGGTTCTGGTCCAACCTTTGTCTTCCTNNAACAGTACAGATG
GCAGCTACACATATAGCCCCGTAAGCTGTGGAATTGGACTTGGTTCCTGGGNNAGAGCCC
CATCTCTGTGGCAGCGGCAGCTATTTACATGGCCTCACAGCCATCAGCTGGAAAGAGGAC
CCACAAGAN
>OriGene 3' read for NM_001514 unedited
TGTACCGCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTTTTGAAAAA
TGTTTTATTCAAGATTACATAATTTCAAATATCTTCCAAAATACAGTTTCCTAGATTGCT
ACAAAAGAAATTTGATAAGAAATTTAAATCATTAAATATGTTTCACTAGTATATACATAC
AGAATGCCAAGCAATAGTATTCAGCCCTGGAATGCGTACCATGTCTTTTGTTTTTTCTCA
TGAAAGGCTCAACCCAGCATTTTGCATAGGCTATGTACAACAGGCAAAGTTTTGTATTCA
AGAATTTGACGTACGCTGCCTCAATTTATAGCCTGTGGCACTTCGCCCACTGGGGTGTCA
CATTTGAAGCCTGTAGGAACCAGATTTGGGGCTCGAGGATAGACCAGTCTATAGGACTGT
CTGATTGTACCATCAGCAACACCAGCATTCTCTCCAATTTCCTTCTGCGTCCTCTCTTAC
CTGATGCCCGCGAGGCCATGCCAATAGCTGTCGTTGCCACAGACACGGCCGCTCCTCCTT
ATGAGTCCCGTCCCCCTCACAGCCTTACGGGCTACATGTGTAGCTGCCATCCGCCCACGG
CTAGGAAGACAAAGCGTCGCCACCGAACCTGGACACGAATTCCCTCAGTTGTCATTCACA
CCCACATTGGTTCCTAGCGCCTTTCAACTCAGTTCCAACCACCGCCCCCCCCCTCTCTAT
CAACCCGGCCCCGGCACATATTTTTTTCACCGCCCTAAGACCCCCCCCCCTGCCTTAAGC
CATATACTATTCTTTTCCCTCCTTTTTCCCCACCACCCCCTCCCCTCCAGGCTTCTCTTG
GAACCAAACTTGTTTCGCACCCATATCCGTTCGCCAACCTCTCTCGCGACGGTTCATGCT
CTCCCCCGCCCGGCAGGTGACTATTTGAAGCACTTATCACTCGCCACAGCACCGCCCCGT
TTTCACACCGCCCTCTAACTGCCCTCCCGTAACCTGGGCCCCGCTTTTCCT
Restriction Sites NotI-NotI     
ACCN NM_001514
Insert Size 1350 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001514.3, NP_001505.1
RefSeq Size 1304 bp
RefSeq ORF 951 bp
Locus ID 2959
UniProt ID Q00403
Domains transcript_fac2, CYCLIN
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Basal transcription factors
Gene Summary This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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