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TFIIB (GTF2B) (NM_001514) Human Untagged Clone
CAT＃: SC321111
GTF2B (untagged)-Human general transcription factor IIB (GTF2B)
CNY 2,400.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TF2B; TFIIB |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_001514.3
ACATTCGCTTCCTGCTTTCGGTGTGTCTGTTGTGTCTTGTTGCGGGCACCGCAGTCGCCG
TGAAGATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATC CAGATGCGATTTTAGTGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCT TGGTTGTAGGTGACCGGGTTATTGATGTGGGATCTGAATGGCGAACTTTCAGCAATGACA AAGCAACAAAAGATCCATCTCGAGTTGGAGATTCTCAGAATCCTCTTCTGAGTGATGGAG ATTTGTCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTGACGAATTTGGCAATT CTAAGTACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGCATTCA AAGAAATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAA ATAATTTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTA TAGCTTCTGCTTGTCTCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAG AAATATGTGCCGTATCACGAATTTCTAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTT TGAAAGCGCTAGAAACCAGTGTGGATTTGATTACAACTGGGGACTTCATGTCCAGGTTCT GTTCCAACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACACATATAGCCCGTAAAG CTGTGGAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGCTATTT ACATGGCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTG GTGTTGCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATC TGTTTCCTACAGACTTCAAATTTGACACCCCAGTGGACAAACTACCACAGCTATAAATTG AGGCAGCTAACGTCAAATTCTTGAATACAAAACTTTGCCTGTTGTACATAGCCTATACAA AATGCTGGGTTGAGCCTTTCATGAGGAAAAACAAAAGACATGGTACGCATTCCAGGGCTG AATACTATTGCTTGGCATTCTGTATGTATATACTAGTGAAACATATTTAATGATTTAAAT TTCTTATCAAATTTCTTTTGTAGCAATCTAGGAAACTGTATTTTGGAAGATATTTGAAAT TATGTAATTCTTGAATAAAACATTTTTCAAAACACAAAAAAAAAAAAAAAAAAAAAAAAA AA |
| Restriction Sites | Please inquire |
| ACCN | NM_001514 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001514.3, NP_001505.1 |
| RefSeq Size | 1304 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 2959 |
| UniProt ID | Q00403 |
| Domains | transcript_fac2, CYCLIN |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Basal transcription factors |
| Gene Summary | This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204607 | GTF2B (Myc-DDK-tagged)-Human general transcription factor IIB (GTF2B) |
CNY 2,400.00 |
|
| RC204607L3 | Lenti ORF clone of Human general transcription factor IIB (GTF2B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204607L4 | Lenti ORF clone of Human general transcription factor IIB (GTF2B), mGFP tagged |
CNY 5,890.00 |
|
| RG204607 | GTF2B (tGFP-tagged) - Human general transcription factor IIB (GTF2B) |
CNY 4,000.00 |
|
| SC110914 | GTF2B (untagged)-Human general transcription factor IIB (GTF2B) |
CNY 2,400.00 |
