Gasdermin D (GSDMD) (NM_024736) Human Untagged Clone
CAT#: SC112028
GSDMD (untagged)-Human gasdermin D (GSDMD), transcript variant 1
CNY 5,488.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DF5L; DFNA5L; FKSG10; GSDMDC1 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC112028 sequence for NM_024736 edited (data generated by NextGen Sequencing)
ATGGGGTCGGCCTTTGAGCGGGTAGTCCGGAGAGTGGTCCAGGAGCTGGACCATGGTGGG GAGTTCATCCCTGTGACCAGCCTGCAGAGCTCCACTGGCTTCCAGCCCTACTGCCTGGTG GTTAGGAAGCCCTCAAGCTCATGGTTCTGGAAACCCCGTTATAAGTGTGTCAACCTGTCT ATCAAGGACATCCTGGAGCCGGATGCCGCGGAACCAGACGTGCAGCGTGGCAGGAGCTTC CACTTCTACGATGCCATGGATGGGCAGATACAGGGCAGCGTGGAGCTGGCAGCCCCAGGA CAGGCAAAGATCGCAGGCGGGGCCGCGGTGTCTGACAGCTCCAGCACCTCAATGAATGTG TACTCGCTGAGTGTGGACCCTAACACCTGGCAGACTCTGCTCCATGAGAGGCACCTGCGG CAGCCAGAACACAAAGTCCTGCAGCAGCTGCGCAGCCGCGGGGACAACGTGTACGTGGTG ACTGAGGTGCTGCAGACACAGAAGGAGGTGGAAGTCACGCGCACCCACAAGCGGGAGGGC TCGGGCCGGTTTTCCCTGCCCGGAGCCACGTGCTTGCAGGGTGAGGGCCAGGGCCATCTG AGCCAGAAGAAGACGGTCACCATCCCCTCAGGCAGCACCCTCGCATTCCGGGTGGCCCAG CTGGTTATTGACTCTGACTTGGACGTCCTTCTCTTCCCGGATAAGAAGCAGAGGACCTTC CAGCCACCCGCGACAGGCCACAAGCGTTCCACGAGCGAAGGCGCCTGGCCACAGCTGCCC TCTGGCCTCTCCATGATGAGGTGCCTCCACAACTTCCTGACAGATGGGGTCCCTGCGGAG GGGGCGTTCACTGAAGACTTCCAGGGCCTACGGGCAGAGGTGGAGACCATCTCCAAGGAA CTGGAGCTTTTGGACAGAGAGCTGTGCCAGCTGCTGCTGGAGGGCCTGGAGGGGGTGCTG CGGGACCAGCTGGCCCTGCGAGCCTTGGAGGAGGCGCTGGAGCAGGGCCAGAGCCTTGGG CCGGTGGAGCCCCTGGACGGTCCAGCAGGTGCTGTCCTGGAGTGCCTGGTGTTGTCCTCC GGAATGCTGGTGCCGGAACTCGCTATCCCTGTTGTCTACCTGCTGGGGGCACTGACCATG CTGAGTGAAACGCAGCACAAGCTGCTGGCGGAGGCGCTGGAGTCGCAGACCCTGTTGGGG CCGCTCGAGCTGGTGGGCAGCCTCTTGGAGCAGAGTGCCCCGTGGCAGGAGCGCAGCACC ATGTCCCTGCCCCCCGGGCTCCTGGGGAACAGCTGGGGCGAAGGAGCACCGGCCTGGGTC TTGCTGGACGAGTGTGGCCTAGAGCTGGGGGAGGACACTCCCCACGTGTGCTGGGAGCCG CAGGCCCAGGGCCGCATGTGTGCACTCTACGCCTCCCTGGCACTGCTATCAGGACTGAGC CAGGAGCCCCACTAG Clone variation with respect to NM_024736.6 >OriGene 5' read for NM_024736 unedited
TTCCGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCAGACG CGCCACCCTCGGGGCGCCGACGGTCACGGAGCATGGGGTCGGCCTTTGAGCGGGTAGTCC GGAGAGTGGTCCAGGAGCTGGACCATGGTGGGGAGTTCATCCCTGTGACCAGCCTGCAGA GCTCCACTGGCTTCCAGCCCTACTGCCTGGTGGTTAGGAAGCCCTCAAGCTCATGGTTCT GGAAACCCCGTTATAAGTGTGTCAACCTGTCTATCAAGGACATCCTGGAGCCGGATGCCG CGGAACCAGACGTGCAGCGTGGCAGGAGCTTCCACTTCTACGATGCCATGGATGGGCAGA TACAGGGCAGCGTGGAGCTGGCAGCCCCAGGACAGGCAAAGATCGCAGGCGGGGCCGCGG TGTCTGACAGCTCCAGCACCTCAATGAATGTGTACTCGCTGAGTGTGGACCCTAACACCT GGCAGACTCTGCTCCATGAGAGGCACCTGCGGCAGCCAGAACACAAAGTCCTGCAGCAGC TGCGCAGCCGCGGGGACAACGTGTACGTGGTGACTGAGGTGCTGCAGACACAGAAGGAGG TGGAAGTCACGCGCACCCACAAGCGGGAGGGCTCGGGCCGGTTTTCCCTGCCCGGAGCCA CGTGCTTGCAGGGTGAGGGCCAGGGCCATCTGAGCCAGAAGAAGACGGTCACCATCCCCT CAGGCAGCACCCTCGCATTCCGGGTGGCCCAGCTGGTTATTGACTCTGACTTGNACGTCC TTCTCTTCCCGGATAAGAGCAGAGGACCTTCCAGCCACCCGCGACAGGCCACAGCGTTCA CGAGCGAAGCGCCTGGCCCAGCTGCCTCTGGCTCTCATGATGAGGTGCCTCACACTTCTG AAGAGGGGTCCTGCGAGGGGCGTTCCTGAGACTTNCAGGCTACGGCAGAGTGGAACATCT CCA >OriGene 3' read for NM_024736 unedited
CTATGGACCGCGCCGCAATTTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTCCTTCGTATGCCACCTTTATTGGGTTTCCCCAACTCCTGGGCCCCATGG TAAACTGGCCACATGGCTACTGGGCTCCTGGCCTTCCTAGGGCTAGCAGCTGGGGGGCAA ACACTCTGCCCTGCTGGAAAGCTGCCAGGCCATGCCCGGGCACAGGCTAGTGGGGCTCCT GGCTCAGTCCTGATAGCAGTGCCAGGGAGGCGTAAAGTGCACACATGCGGCCCTGGGCCT GCGGCTCCCAGCACACGTGGGGAGTGTCCTCCCCCAGCTTTAGGCCACACTCGTCCAGCA AGACCCAGGCCGGTGCTCCTTCGCCCCAGCTGTTCCCCAGGAGCCCGGGGGGCAGGGACA TGGTGCTGCGCTCCTGCCACGGGGCACTCTGCTCCAAGAGGCTGCCCACCAGCTCGAGCG GCCCCAACAGGGTCTGCGACTCCAGCGCCTCCGCCAGCAGCTTGTGCTGCGTTTCACTCA GCATGGTCAGTGCCCCCAGCAGGTAGACAACAGGGATAGCGAGTTCCGGCACCAGCATTC CGGAGGACAACACCAGGCACTCCAGGACAGCACCTGCTGGACCGTCCAGGGGCTCCACCG GCCCAAGGCTCTGGCCCTGGCTCAGCGCCTCCTCCAAGCTCGCAGGGCCAGCTGGTCCCG CAGCACCCCCTTCAGGCCCCTCAGCAGCAGCTGGCAAAGCTTCTGTCCAAAAGCTCCAGT TCCTTTGGAGATGGTCTCCACCTCTGCCCGTAGGCCCTGNAAGTCTCAATGAACGCCCCC TCCGCAGGGACCCATCTGTCAGNAAGTTGTGGAAGCACCTTCTCATGGAAAGCCCAAGGG CANCTGTGGCCAGGCCCCTCCCTCGTGGAACCCTT |
Restriction Sites | NotI-NotI |
ACCN | NM_024736 |
Insert Size | 1750 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_024736.3, NP_079012.1 |
RefSeq Size | 1823 bp |
RefSeq ORF | 1455 bp |
Locus ID | 79792 |
UniProt ID | P57764 |
Domains | DFNA5 |
Gene Summary | Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203399 | GSDMD (Myc-DDK-tagged)-Human gasdermin D (GSDMD), transcript variant 1 |
CNY 5,488.00 |
|
RC203399L1 | Lenti ORF clone of Human gasdermin D (GSDMD), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
RC203399L2 | Lenti ORF clone of Human gasdermin D (GSDMD), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC203399L3 | Lenti ORF clone of Human gasdermin D (GSDMD), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
RC203399L4 | Lenti ORF clone of Human gasdermin D (GSDMD), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
RG203399 | GSDMD (tGFP-tagged) - Human gasdermin D (GSDMD), transcript variant 1 |
CNY 7,088.00 |
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