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ATP6V0A2 (NM_012463) Human Untagged Clone
CAT＃: SC115366
ATP6V0A2 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2)
CNY 9,416.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D; J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115366 sequence for NM_012463 edited (data generated by NextGen Sequencing)
ATGGGGTCCCTGTTCCGGAGCGAGACCATGTGCCTGGCGCAGCTCTTCCTGCAGTCGGGC ACGGCCTACGAGTGCCTCAGCGCCCTGGGCGAGAAAGGCCTGGTCCAGTTCCGAGACCTC AACCAGAACGTAAGTTCTTTCCAAAGAAAATTTGTTGGTGAGGTGAAGAGGTGTGAAGAG CTAGAGCGAATATTGGTGTATTTGGTACAGGAAATTAATAGAGCTGATATTCCCCTTCCT GAAGGAGAGGCCAGCCCTCCTGCGCCACCCCTGAAACAGGTTCTAGAAATGCAGGAGCAG TTGCAGAAGCTCGAGGTTGAACTGAGAGAAGTCACTAAGAACAAGGAGAAACTGAGGAAA AACTTGCTGGAACTGATAGAGTACACTCACATGCTGAGAGTGACAAAGACCTTTGTGAAA CGCAACGTTGAGTTTGAACCCACTTATGAAGAATTCCCTTCCTTAGAGAGCGATTCTTTG TTGGATTACAGCTGTATGCAGAGGCTGGGAGCAAAACTGGGATTTGTGTCTGGCCTAATT AACCAAGGAAAAGTGGAAGCATTTGAAAAAATGTTGTGGAGAGTCTGCAAAGGGTACACC ATCGTGTCCTATGCAGAACTGGATGAATCCCTTGAAGACCCTGAAACAGGGGAAGTCATA AAATGGTATGTCTTTTTAATATCCTTTTGGGGAGAGCAGATTGGCCACAAGGTTAAGAAG ATATGTGATTGCTACCACTGCCACGTGTACCCCTATCCAAACACAGCCGAGGAGCGGAGG GAGATCCAGGAGGGGCTGAACACCCGCATCCAGGATCTCTACACTGTACTGCACAAAACC GAGGACTATTTGAGGCAAGTGCTATGTAAAGCCGCCGAGTCTGTCTACAGCCGTGTGATC CAGGTGAAGAAAATGAAGGCCATCTATCACATGCTGAACATGTGCAGCTTTGACGTGACC AACAAGTGCCTCATTGCTGAGGTCTGGTGTCCCGAGGCGGATCTGCAGGACCTGCGCCGG GCACTGGAGGAGGGCTCGAGAGAGAGTGGTGCTACAATCCCCTCATTCATGAATATAATC CCCACAAAAGAAACACCCCCCACTCGGATCCGCACCAACAAATTCACCGAGGGATTTCAG AACATCGTGGATGCTTATGGAGTCGGAAGCTACAGAGAAGTCAATCCAGCTCTCTTTACC ATCATCACCTTCCCGTTTTTATTTGCTGTGATGTTTGGAGACTTCGGACATGGCTTTGTG ATGTTTTTATTTGCCCTCTTGTTGGTGTTAAATGAAAATCATCCCAGACTAAATCAGTCA CAAGAGATCATGAGGATGTTTTTTAATGGCCGGTACATCCTCCTGCTGATGGGGCTGTTC TCAGTGTACACTGGCCTCATCTACAACGACTGCTTTTCAAAGTCAGTCAACCTGTTCGGC TCTGGGTGGAACGTGTCGGCCATGTACAGCTCCAGCCACCCACCCGCAGAGCATAAGAAG ATGGTGCTTTGGAACGACAGCGTCGTTAGACACAACAGCATTTTGCAGCTGGATCCAAGC ATTCCTGGAGTGTTCCGAGGCCCTTATCCCCTTGGCATTGATCCTATTTGGAACTTGGCC ACAAATCGCCTCACTTTTCTAAACTCTTTCAAAATGAAAATGTCCGTGATTTTAGGAATC ATTCATATGACTTTTGGAGTCATTCTGGGAATATTTAACCACTTGCACTTCAGGAAGAAG TTCAACATTTACCTGGTTTCCATCCCGGAACTTCTCTTCATGCTCTGTATCTTTGGATAC CTTATATTTATGATTTTCTACAAGTGGCTGGTTTTTTCAGCAGAAACCTCCAGAGTTGCT CCCAGCATTCTGATTGAATTTATTAACATGTTTTTATTCCCAGCCAGTAAAACAAGTGGC CTTTACACAGGGCAGGAGTATGTCCAGAGAGTGCTGCTGGTTGTCACAGCATTGTCTGTC CCTGTCCTCTTCTTGGGAAAGCCACTGTTTTTGTTGTGGCTTCACAATGGGCGTAGTTGC TTCGGGGTGAACCGGAGTGGCTACACACTTATAAGGAAAGATAGTGAGGAAGAAGTTTCA TTGCTGGGAAGCCAAGATATAGAAGAGGGAAATCACCAGGTGGAAGATGGATGTAGAGAA ATGGCGTGTGAAGAGTTTAATTTTGGAGAAATATTAATGACCCAAGTAATCCATTCCATC GAGTACTGTCTGGGATGCATCTCCAACACCGCCTCCTACCTGAGGCTCTGGGCGCTTAGC CTGGCTCACGCACAGTTGTCTGATGTCCTGTGGGCCATGCTGATGCGCGTGGGCCTCCGC GTTGACACCACCTATGGCGTCTTGCTACTGCTCCCGGTTATCGCGCTCTTTGCAGTTTTG ACCATTTTCATCCTTCTGATCATGGAAGGGCTTTCTGCGTTTCTTCACGCCATACGCCTC CACTGGGTAGAATTTCAGAACAAATTCTACGTTGGTGCAGGCACCAAATTTGTTCCTTTC TCATTCAGTCTACTTTCATCAAAGTTCAATAACGACGACAGTGTGGCATGA Clone variation with respect to NM_012463.3 426 t=>c;471 t=>c;1515 t=>c >OriGene 5' read for NM_012463 unedited
TCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCGGACTGC TGTGGCGGCAGCTGGAGCGGCGGCCGCGGTGGCAGAACCGGGGGCGGCCGCTGCAGTCTG GAGCCCCATAGTGCGGGGCCGCGGCCAGGCCACAGGAAGAGCTCGAGGCCCGGGCCGCAC CGGCTGAGTGTGCGGGCCCGCGCGGCTCGGAGCCGCCGCCGCCCATCGAGCCCCTCCGGG CGCGGGTCGGCCCGCCATGGGGTCCCTGTTCCGGAGCGAGACCATGTGCCTGGCGCAGCT CTTCCTGCAGTCGGGCACGGCCTACGAGTGCCTCAGCGCCCTGGGCGAGAAAGGCCTGGT CCAGTTCCGAGACCTCAACCAGAACGTAAGTTCTTTCCAAAGAAAATTTGTTGGTGAGGT GAAGAGGTGTGAAGAGCTAGAGCGAATATTGGTGTATTTGGTACAGGAAATTAATAGAGC TGATATTCCCCTTCCTGAAGGAGAGGCCAGCCCTCCTGCGCCACCCCTGAAACAGGTTCT AGAAATGCAGGAGCAGTTGCAGAAGCTCGAGGTTGAACTGAGAGAAGTCACTAAGAACAA GGAGAAACTGAGGAAAAACTTGCTGGAACTGATAGAGTACACTCACATGCTGAGAGTGAC AAAGACCTTTGTGAAACGCAACGTTGAGTTTGAACCCACTTATGAAGAATTCCCTTCCTT AGAGAGCGATTCTTTGTTGGATTACAGCTGTATGCAGNAGCTGGGAGCAAAACTGGATTT GTGTCTGGCCTAATAACCAAGGAAAGTGGAGCATTGAAAAATGTTGTGGAGAGTCTNGCA AGGTACACCATCGTGTCTATGCAAACTGGATGATCCCTTGAGACCTGAACAGGGGAGTCA TAAGGNATGTCTTTTAATATCCTTTGGGAGACAGATGGCCACAGGTAGAAGATTGTGATG CTAC >OriGene 3' read for NM_012463 unedited
NNTTCGGTACACCTAAGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTT TTTTTTTTTTTCATGGAAAAATATCTTCATTTATATTTTGTTACAAAAATATACAAAATA CTGCTTTTCACCTTTCCTTCACTCAGTATAAACCCTTCTGGTTCAGTGACATCAGCCAAG CTGTCACTAAATTCAAATCATGTCCGCATTATATCTTCTACAGTCACACTGGGTTTTAAG GCTCAGCATACAATTTATCTTCCAAACATACAATTATCATAACTGGTTTTATGTAAGGGT AACAGGATAAGAGTCTTTAAAAAAATATACAAGTGGGATAACCATTTGTTGTATGTCATT TTATCCCATTGTCCCCAATTACTAAAACAAGACAAAATTGTAAAAACAATCCTCTACTTG TAATTCTAGGTAGCACAGGATAAAAAATATTTGCATATTTTGGCCAGGCATGGTGGCTCA CGCCTGTAATCCCAGCACTTAGGGAGGCCGAGGCAGGTGGATCACTTGAGGTCAGAAGTT CGAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACAAAAATACAAAAAAATTAGC TGGTCACGGTGGCGCATGCCTGTAATCCCAGTTACTCGGGAGGCTGAGACAGGAGAATCA CCTGAATCCAGGAGGCGGAAGTTGCAATGAGACACGATCGTGCCATTGCACTCCAGCCTA CGTGACACAGCAAGACTTCATCTCAAAAAAAAAAAAAGTACATATTTTTAAATCCAGCAG ATAAATGTGAAATGTTTTGATTACCCATCATTAAATTTTTTAAAAAACTATCCCTACTTC AGCTGTGGNAAAAATTATCTGGCAACTTCAGGTGACAGATAACTCTGTTGTTTGGTTTAA AAAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012463 |
| Insert Size | 4540 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012463.2, NP_036595.2 |
| RefSeq Size | 4681 bp |
| RefSeq ORF | 2571 bp |
| Locus ID | 23545 |
| UniProt ID | Q9Y487 |
| Domains | V_ATPase_sub_a |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| Gene Summary | The protein encoded by this gene is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome. [provided by RefSeq, Jul 2009] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Molecular mechanisms of cutis laxa– and distal renal tubular acidosis–causing mutations in V-ATPase a subunits, ATP6V0A2 and ATP6V0A4
,null,
The Journal of Biological Chemistry
,PubMed ID 29311258
[ATP6V0A2]
|
|
N-linked Glycosylation of a Subunit Isoforms is Critical for Vertebrate Vacuolar H(+) -ATPase (V-ATPase) Biosynthesis
,Esmail, S;Kartner, N;Yao, Y;Kim, JW;Reithmeier, RAF;Manolson, MF;,
J. Cell. Biochem.
,PubMed ID 28661051
[ATP6V0A2]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209819 | ATP6V0A2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 9,400.00 |
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| RC209819L1 | Lenti-ORF clone of ATP6V0A2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 11,800.00 |
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| RC209819L2 | Lenti-ORF clone of ATP6V0A2 (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 9,030.00 |
|
| RC209819L3 | Lenti-ORF clone of ATP6V0A2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 9,030.00 |
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| RC209819L4 | Lenti-ORF clone of ATP6V0A2 (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 9,030.00 |
|
| RG209819 | ATP6V0A2 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2) |
CNY 7,890.00 |
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