APPL (APPL1) (NM_012096) Human Untagged Clone
CAT#: SC115543
APPL1 (untagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1)
CNY 5,200.00
Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | APPL; DIP13alpha; MODY14 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC115543 sequence for NM_012096 edited (data generated by NextGen Sequencing)
ATGCCGGGGATCGACAAGCTGCCCATCGAGGAGACGCTGGAGGACAGCCCGCAGACAAGG TCTTTACTAGGTGTATTTGAAGAAGATGCCACAGCTATTTCCAACTATATGAACCAGTTG TATCAAGCTATGCATCGGATTTATGATGCACAGAATGAATTAAGTGCAGCAACACACCTG ACCTCAAAACTTTTAAAAGAATATGAAAAACAGCGTTTTCCATTGGGAGGTGATGATGAA GTTATGAGCTCTACATTGCAACAGTTTTCAAAAGTTATAGATGAGCTTAGCTCTTGTCAT GCAGTGCTTTCAACTCAACTTGCTGATGCCATGATGTTCCCCATTACCCAGTTTAAAGAA AGAGATCTGAAAGAAATACTAACATTAAAGGAAGTATTTCAGATTGCAAGTAATGATCAT GATGCTGCGATTAATAGATATAGCCGTTTATCAAAAAAAAGAGAAAATGACAAGGTGAAG TATGAAGTAACAGAAGATGTGTACACATCCAGAAAGAAACAACACCAGACCATGATGCAT TATTTTTGTGCATTAAATACTCTTCAGTACAAGAAGAAAATAGCATTGTTAGAACCTCTA CTTGGGTACATGCAAGCTCAGATAAGTTTCTTTAAGATGGGTTCTGAAAATCTTAATGAA CAACTGGAAGAATTTTTAGCTAATATTGGAACAAGCGTTCAGAATGTTCGCAGGGAAATG GACAGTGATATAGAGACCATGCAACAGACAATAGAGGATTTGGAAGTAGCCAGTGATCCC TTATATGTGCCTGACCCAGACCCCACCAAATTTCCTGTTAATCGAAATTTAACCCGAAAG GCTGGATACCTTAATGCTAGGAATAAAACAGGCTTGGTGTCATCTACCTGGGACAGACAG TTTTACTTCACGCAGGGTGGAAATTTAATGAGTCAGGCCCGTGGGGATGTAGCAGGAGGC CTGGCCATGGACATAGACAACTGTTCAGTGATGGCTGTGGACTGTGAAGACAGACGATAT TGTTTTCAGATCACCTCTTTCGATGGAAAAAAATCTTCAATTTTGCAAGCAGAGAGTAAA AAAGATCATGAAGAGTGGATCTGTACAATAAACAACATATCTAAACAAATATACTTAAGT GAAAATCCAGAGGAAACTGCTGCACGAGTAAATCAATCAGCTCTGGAAGCTGTCACTCCT TCCCCATCTTTCCAGCAGAGGCACGAGAGCCTGCGGCCAGCAGCAGGACAATCTCGGCCA CCGACAGCTCGAACCAGCAGTTCAGGATCCTTAGGATCTGAGTCTACAAATTTGGCTGCC CTCTCTCTAGATTCTCTTGTTGCCCCAGACACCCCAATACAGTTTGACATAATTTCTCCT GTGTGTGAAGATCAGCCTGGCCAGGCAAAAGCCTTTGGCCAGGGAGGCAGGCGTACAAAT CCATTTGGAGAATCTGGAGGAAGTACAAAATCTGAAACTGAAGATTCTATTCTTCATCAG TTATTTATTGTCCGATTCCTTGGTTCAATGGAGGTGAAATCAGATGACCATCCAGATGTT GTTTATGAAACTATGCGCCAAATCTTAGCTGCCCGGGCCATCCATAACATCTTTCGTATG ACAGAATCGCATTTATTAGTCACTTGTGACTGTTTAAAGTTAATTGATCCACAGACACAA GTTACAAGGCTCACGTTTCCATTACCTTGTGTAGTTTTGTATGCTACACACCAGGAAAAT AAGCGCCTTTTTGGATTTGTTCTTCGGACATCAAGCGGGAGAAGTGAAAGTAATCTGTCA TCAGTCTGCTATATATTTGAGTCAAACAATGAGGGGGAAAAGATATGTGATTCTGTTGGA CTGGCAAAACAGATAGCTTTGCATGCTGAACTGGATCGTAGGGCATCAGAAAAACAAAAA GAAATAGAGAGAGTAAAAGAGAAGCAACAGAAAGAACTCAATAAACAAAAACAGATTGAA AAGGACTTGGAAGAACAAAGTCGGTTGATAGCTGCTTCCAGTAGACCAAACCAAGCCAGT AGTGAGGGGCAGTTTGTTGTCCTTAGCAGTAGCCAGTCAGAAGAGAGTGATTTGGGAGAA GGAGGAAAGAAGAGAGAATCAGAAGCATAA Clone variation with respect to NM_012096.2 >OriGene 5' read for NM_012096 unedited
TCGGAATTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCTGGNAGA AGCTGTGCGGGCGGGGACGGCTGCAGCCCTTGCCGGAGAGGGCGGGCCGGGGTCAGCTGC GGCGGGCGGGCCGGCGCGGGGAGCTGTGGGCGGCAGCTGCGTCTCCTGCCACCGCCCTCC CTCCGCCACGATGCCGGGGATCGACAAGCTGCCCATCGAGGAGACGCTGGAGGACAGCCC GCAGACAAGGTCTTTACTAGGTGTATTTGAAGAAGATGCCACAGCTATTTCCAACTATAT GAACCAGTTGTATCAAGCTATGCATCGGATTTATGATGCACAGAATGAATTAAGTGCAGC AACACACCTGACCTCAAAACTTTTAAAAGAATATGAAAAACAGCGTTTTCCATTGGGAGG TGATGATGAAGTTATGAGCTCTACATTGCAACAGTTTTCAAAAGTTATAGATGAGCTTAG CTCTTGTCATGCAGTGCTTTCAACTCAACTTGCTGATGCCATGATGTTCCCCATTACCCA GTTTAAAGAAAGAGATCTGAAAGAAATACTAACATTAAAGGAAGTATTTCAGATTGCAAG TAATGATCATGATGCTGCGATTAATAGATATAGCCGTTTATCAAAAAAAAGAGAAAATGA CAAGGGTGAAGTATGAAGTAACAGAAAGATGTGTACACATCCAGAAAGAAACAACACCAG ACCATGATGCATTATTTTTGTGCATTANATACTCTTCAGTACAAGAAGAANATAGCATTG TTAGAACCTCTACTTGNGTACATGNCAGCTCAGATAAGTTTCTTAAGAATGGGGTTCTGA AAATCTTAAATGACACTGGNAGAATTTTTAGCTAATATGGAACAGCGTTCAAATGGTCGC AGGAATGGACAGTGANTAGAGACATGCACAGACATAAGTTTTTGGAGTGC >OriGene 3' read for NM_012096 unedited
GCTATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTTTATT CAGGGGGCAGGTTTTACAACATACTAGCTCTTAGGATCTCTCAGCATTAATAGGATTCAA ATTTTAAATAATATGGTTCCTAGATATAATGCAAAATTAACTCAAATGCCTTTACCATAT ACGATTTTATCTTCCCTAGGATGCACATCCTACTGACCTTTTCCCAAGTATAGTGGAAAC ACATTTACCACCCCTAGATTGTAGAGCCTGAGGATTCTTTTGAAATTAAACAATAATCAT TGCAGATGTGGCAAAAACTAGACTAGCTTTAGGGACTCTGTTCAAGATAATAGCTTTAGT CCCTGGTTGGCAAGTTCTCAAAATTTTTAAAGGTAGAAGGAATAACTGGTTTCCTATCTA CTGACTTCATTGCCATAGTTACAAACATACTGTTCAAATAGGTAACAGAATGCATTGTAG AAGTAGGCAGGCTTAATATCTTTTCTTTTTAAAAAGGAGTTTTGTTGATTTCTAACACCA TTAAGTGTAAGTCTGAATGTGAGAAAAGATATCCCAAAATCGAATCCAGTTACCCTCAAA GCATCTCAGAACTAGAAGGGATTTCAAAGGAGGGTAACAATCAGCTTTACCACTAGGCAG AAACCCATGGGCAGCCTTTTAATAACNAAAAAGAGCTTATTAAAACAGATACTTAAATCT CTGCAGTAATGGAATAGTTCATATAGTTCAACAGTAATGGAAACAAAAGCTGATCATCTA ANATTGNTAAACCCACTCATAACTTCTATTTAGCATTGNAATGCACTTCGATCTGACCTA TGGAGATGAGGGTTTATTTATGTAGAAATNCTGTGAACATGACTTAAACGAACTACAGGT CTGTCAGCATCCCTAGTCTGCCAGNGCCTAACTTTANCTACCTTGACCCAGGTGCTGATA AGGN |
Restriction Sites | NotI-NotI |
ACCN | NM_012096 |
Insert Size | 5270 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_012096.1, NP_036228.1 |
RefSeq Size | 5970 bp |
RefSeq ORF | 2130 bp |
Locus ID | 26060 |
UniProt ID | Q9UKG1 |
Domains | PH, PID |
Protein Pathways | Colorectal cancer, Pathways in cancer |
Gene Summary | The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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APPL1 promotes glucose uptake in response to mechanical stretch via the PKC?-non-muscle myosin IIa pathway in C2C12 myotubes
,Saito, T;Okada, S;Shimoda, Y;Tagaya, Y;Osaki, A;Yamada, E;Shibusawa, R;Nakajima, Y;Ozawa, A;Satoh, T;Mori, M;Yamada, M;,
Cell. Signal.
,PubMed ID 27478065
[APPL1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206021 | APPL1 (Myc-DDK-tagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1) |
CNY 5,192.00 |
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RC206021L1 | Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), Myc-DDK-tagged |
CNY 7,592.00 |
|
RC206021L2 | Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), mGFP tagged |
CNY 5,890.00 |
|
RC206021L3 | Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206021L4 | Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), mGFP tagged |
CNY 5,890.00 |
|
RG206021 | APPL1 (tGFP-tagged) - Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1) |
CNY 6,792.00 |