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CD146 (MCAM) (NM_006500) Human Untagged Clone
CAT＃: SC116067
MCAM (untagged)-Human melanoma cell adhesion molecule (MCAM)
CNY 7,224.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD146; HEMCAM; MelCAM; METCAM; MUC18 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116067 sequence for NM_006500 edited (data generated by NextGen Sequencing)
ATGGGGCTTCCCAGGCTGGTCTGCGCCTTCTTGCTCGCCGCCTGCTGCTGCTGTCCTCGC GTCGCGGGTGTGCCCGGAGAGGCTGAGCAGCCTGCGCCTGAGCTGGTGGAGGTGGAAGTG GGCAGCACAGCCCTTCTGAAGTGCGGCCTCTCCCAGTCCCAAGGCAACCTCAGCCATGTC GACTGGTTTTCTGTCCACAAGGAGAAGCGGACGCTCATCTTCCGTGTGCGCCAGGGCCAG GGCCAGAGCGAACCTGGGGAGTACGAGCAGCGGCTCAGCCTCCAGGACAGAGGGGCTACT CTGGCCCTGACTCAAGTCACCCCCCAAGACGAGCGCATCTTCTTGTGCCAGGGCAAGCGC CCTCGGTCCCAGGAGTACCGCATCCAGCTCCGCGTCTACAAAGCTCCGGAGGAGCCAAAC ATCCAGGTCAACCCCCTGGGCATCCCTGTGAACAGTAAGGAGCCTGAGGAGGTCGCTACC TGTGTAGGGAGGAACGGGTACCCCATTCCTCAAGTCATCTGGTACAAGAATGGCCGGCCT CTGAAGGAGGAGAAGAACCGGGTCCACATTCAGTCGTCCCAGACTGTGGAGTCGAGTGGT TTGTACACCTTGCAGAGTATTCTGAAGGCACAGCTGGTTAAAGAAGACAAAGATGCCCAG TTTTACTGTGAGCTCAACTACCGGCTGCCCAGTGGGAACCACATGAAGGAGTCCAGGGAA GTCACCGTCCCTGTTTTCTACCCGACAGAAAAAGTGTGGCTGGAAGTGGAGCCCGTGGGA ATGCTGAAGGAAGGGGACCGCGTGGAAATCAGGTGTTTGGCTGATGGCAACCCTCCACCA CACTTCAGCATCAGCAAGCAGAACCCCAGCACCAGGGAGGCAGAGGAAGAGACAACCAAC GACAACGGGGTCCTGGTGCTGGAGCCTGCCCGGAAGGAACACAGTGGGCGCTATGAATGT CAGGGCCTGGACTTGGACACCATGATATCGCTGCTGAGTGAACCACAGGAACTACTGGTG AACTATGTGTCTGACGTCCGAGTGAGTCCCGCAGCCCCTGAGAGACAGGAAGGCAGCAGC CTCACCCTGACCTGTGAGGCAGAGAGTAGCCAGGACCTCGAGTTCCAGTGGCTGAGAGAA GAGACAGGCCAGGTGCTGGAAAGGGGGCCTGTGCTTCAGTTGCATGACCTGAAACGGGAG GCAGGAGGCGGCTATCGCTGCGTGGCGTCTGTGCCCAGCATACCCGGCCTGAACCGCACA CAGCTGGTCAACGTGGCCATTTTTGGCCCCCCTTGGATGGCATTCAAGGAGAGGAAGGTG TGGGTGAAAGAGAATATGGTGTTGAATCTGTCTTGTGAAGCGTCAGGGCACCCCCGGCCC ACCATCTCCTGGAACGTCAACGGCACGGCAAGTGAACAAGACCAAGATCCACAGCGAGTC CTGAGCACCCTGAATGTCCTCGTGACCCCGGAGCTGTTGGAGACAGGTGTTGAATGCACG GCCTCCAACGACCTGGGCAAAAACACCAGCATCCTCTTCCTGGAGCTGGTCAATTTAACC ACCCTCACACCAGACTCCAACACAACCACTGGCCTCAGCACTTCCACTGCCAGTCCTCAT ACCAGAGCCAACAGCACCTCCACAGAGAGAAAGCTGCCGGAGCCGGAGAGCCGGGGCGTG GTCATCGTGGCTGTGATTGTGTGCATCCTGGTCCTGGCGGTGCTGGGCGCTGTCCTCTAT TTCCTCTATAAGAAGGGCAAGCTGCCGTGCAGGCGCTCAGGGAAGCAGGAGATCACGCTG CCCCCGTCTCGTAAGAGCGAACTTGTAGTTGAAGTTAAGTCAGATAAGCTCCCAGAAGAG ATGGGCCTCCTGCAGGGCAGCAGCGGTGACAAGAGGGCTCCGGGAGACCAGGGAGAGAAA TACATCGATCTGAGGCATTAG Clone variation with respect to NM_006500.2 1800 a=>g >OriGene 5' read for NM_006500 unedited
TAACGTCAAATTTTGTATACGACTCCTATAGGCGGCACGCGAATTCGCACGAGAGGGGCT TCCCAGGCTGGTCTGCGCCTTCTTGCTCGCCGCCTGCTGCTGCTGTCCTCGCGTCGCGGG TGTGCCCGGAGAGGCTGAGCAGCCTGCGCCTGAGCTGGTGGAGGTGGAAGTGGGCAGCAC AGCCCTTCTGAAGTGCGGCCTCTCCCAGTCCCAAGGCAACCTCAGCCATGTCGACTGGTT TTCTGTCCACAAGGAGAAGCGGACGCTCATCTTCCGTGTGCGCCAGGGCCAGGGCCAGAG CGAACCTGGGGAGTACGAGCAGCGGCTCAGCCTCCAGGACAGAGGGGCTACTCTGGCCCT GACTCAAGTCACCCCCCAAGACGAGCGCATCTTCTTGTGCCAGGGCAAGCGCCCTCGGTC CCAGGAGTACCGCATCCAGCTCCGCGTCTACAAAGCTCCGGAGGAGCCAAACATCCAGGT CAACCCCCTGGGCATCCCTGTGAACAGTAAGGAGCCTGAGGAGGTCGCTACCTGTGTAGG GAGGAACGGGTACCCCATTCCTCAAGTCATCTGGTACAAGAATGGCCGGCCTCTGAAGGA GGAGAAGAACCGGGTCCACATTCAGTCGTCCCAGACTGTGGAGTCGAGTGGTTTGTACAC CTTGCAGAGTATTCTGAAGGCACAGCTGGTTAAAGAAGACAAAGATGCCCAGTTTTACTG TGAGCTCAACTACCGGCTGCCCAGTGGGAACCACATGAAGGAGTCCAGGGAAGTCACCGT CCCTGTTTTCTNACCCGACAGATAAGTGTGGCTGGAAGTGGAGCCCGTGGGAATGCTGAA NGAGGGGACCGCGTGGAATCATGTGTTTGGGCTGATGGCACCCTNCACCACACTTCAGCA TCAGCAAGCATGATCCCAGCACC >OriGene 3' read for NM_006500 unedited
CTATGTAACGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTGATTTCTGGGG CAATTAAGCTTTATTTTTCATATATATATATATTCTTCATATATATATATACATACATAT ATAAAGGAAACAATTTGCAAATTTACACACCTGACAAAACCATATATACACACATATGTA TGCATACACACAGACAGACACACACACCCGAAGCTCTAGCCAGGCCCGTTTTCCATCCCT AAGTACCATTCTCTCATTTGGGCCCTTCTAAGGTTGGGGCCCTGAGCTTGGTTTGTAGAA GTTTGGTGCTAATATAACCATAGCTTTAATCCCCATGAAGGACAGTGTAGACCTCATCTT TGTCTGCTCCCCGCTGCCTTTCAGTTTTACGTGATCCATCAAGAGGGCTATGGGAGCCAA GTGAACACGGGGGATTGAGGCTAATTCACCTGAACTCGAAAACAGCGCCCAGCTTCCTCA CTGCAAGCACGTGTCTTTTCTTTTTTTTTTCTCGAGACGGAGTCTCGCTGTGTTGCCCAA GCTGGAGTGCAGTGGCACGGTCTCGGCTCACTGCAAGCTCCACCTCCTGGATTCATACCA TTCTCCTGCTTCAAGCCTTCGAGTAGCTGGGACTATATGTGCCAACCACTACGCCTAGCT AACTCCTTCTAGATTTTTAATACACACAGGCTTTCACCGGGTTAACCACGATGGACTTGT CCTGAATTTGTGATCCGCCCGCCTCGGCCCACAAAAGGCCGGGATTACAGGTGTGAGACA CCACACCTGGCCCCGGAACGTATTTTTAAAGAAGACACCAATTCTTGGTTTTGCACAAGA AAATAGTCAAGGATATTTGTACAGCACAATGAGGGTGGGGCCCACCCTGCAACTGCTATG GATGCTTCGGGGGGTTCTGCAGCGGNGGTACAAACCACTAAACTTATCGGCCCCGCACCG GGAACCTCTTAACGTGGCCCGGGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006500 |
| Insert Size | 3460 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006500.2, NP_006491.2 |
| RefSeq Size | 3332 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 4162 |
| UniProt ID | P43121 |
| Domains | ig, IGc2, IG |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Gene Summary | Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived Xenografts
,Crépin, R;Gentien, D;Duché, A;Rapinat, A;Reyes, C;Némati, F;Massonnet, G;Decaudin, D;Djender, S;Moutel, S;Desrumeaux, K;Cassoux, N;Piperno-Neumann, S;Amigorena, S;Perez, F;Roman Roman, S;de Marco, A;,
Pigment Cell Melanoma Res
,PubMed ID 28140525
[MCAM]
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Identification of MCAM/CD146 as the Target Antigen of a Human Monoclonal Antibody that Recognizes Both Epithelioid and Sarcomatoid Types of Mesothelioma
,Scott Bidlingmaier, Jiang He, Yong Wang, Feng An, Jinjin Feng, Dario Barbone, Dongwei Gao, Ben Franc, V. Courtney Broaddus, and Bin Liu,
Cancer Res., Feb 2009; 69: 1570 - 1577.
[MCAM]
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