CD146 (MCAM) (NM_006500) Human Untagged Clone

CAT#: SC116067

MCAM (untagged)-Human melanoma cell adhesion molecule (MCAM)



  "NM_006500" in other vectors (6)

CNY 7,224.00


货期*
现货

规格
    • 10 ug

Cited in 2 publications.

Product images

经常一起买 (3)
MCAM mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CD146; HEMCAM; MelCAM; METCAM; MUC18
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116067 sequence for NM_006500 edited (data generated by NextGen Sequencing)
ATGGGGCTTCCCAGGCTGGTCTGCGCCTTCTTGCTCGCCGCCTGCTGCTGCTGTCCTCGC
GTCGCGGGTGTGCCCGGAGAGGCTGAGCAGCCTGCGCCTGAGCTGGTGGAGGTGGAAGTG
GGCAGCACAGCCCTTCTGAAGTGCGGCCTCTCCCAGTCCCAAGGCAACCTCAGCCATGTC
GACTGGTTTTCTGTCCACAAGGAGAAGCGGACGCTCATCTTCCGTGTGCGCCAGGGCCAG
GGCCAGAGCGAACCTGGGGAGTACGAGCAGCGGCTCAGCCTCCAGGACAGAGGGGCTACT
CTGGCCCTGACTCAAGTCACCCCCCAAGACGAGCGCATCTTCTTGTGCCAGGGCAAGCGC
CCTCGGTCCCAGGAGTACCGCATCCAGCTCCGCGTCTACAAAGCTCCGGAGGAGCCAAAC
ATCCAGGTCAACCCCCTGGGCATCCCTGTGAACAGTAAGGAGCCTGAGGAGGTCGCTACC
TGTGTAGGGAGGAACGGGTACCCCATTCCTCAAGTCATCTGGTACAAGAATGGCCGGCCT
CTGAAGGAGGAGAAGAACCGGGTCCACATTCAGTCGTCCCAGACTGTGGAGTCGAGTGGT
TTGTACACCTTGCAGAGTATTCTGAAGGCACAGCTGGTTAAAGAAGACAAAGATGCCCAG
TTTTACTGTGAGCTCAACTACCGGCTGCCCAGTGGGAACCACATGAAGGAGTCCAGGGAA
GTCACCGTCCCTGTTTTCTACCCGACAGAAAAAGTGTGGCTGGAAGTGGAGCCCGTGGGA
ATGCTGAAGGAAGGGGACCGCGTGGAAATCAGGTGTTTGGCTGATGGCAACCCTCCACCA
CACTTCAGCATCAGCAAGCAGAACCCCAGCACCAGGGAGGCAGAGGAAGAGACAACCAAC
GACAACGGGGTCCTGGTGCTGGAGCCTGCCCGGAAGGAACACAGTGGGCGCTATGAATGT
CAGGGCCTGGACTTGGACACCATGATATCGCTGCTGAGTGAACCACAGGAACTACTGGTG
AACTATGTGTCTGACGTCCGAGTGAGTCCCGCAGCCCCTGAGAGACAGGAAGGCAGCAGC
CTCACCCTGACCTGTGAGGCAGAGAGTAGCCAGGACCTCGAGTTCCAGTGGCTGAGAGAA
GAGACAGGCCAGGTGCTGGAAAGGGGGCCTGTGCTTCAGTTGCATGACCTGAAACGGGAG
GCAGGAGGCGGCTATCGCTGCGTGGCGTCTGTGCCCAGCATACCCGGCCTGAACCGCACA
CAGCTGGTCAACGTGGCCATTTTTGGCCCCCCTTGGATGGCATTCAAGGAGAGGAAGGTG
TGGGTGAAAGAGAATATGGTGTTGAATCTGTCTTGTGAAGCGTCAGGGCACCCCCGGCCC
ACCATCTCCTGGAACGTCAACGGCACGGCAAGTGAACAAGACCAAGATCCACAGCGAGTC
CTGAGCACCCTGAATGTCCTCGTGACCCCGGAGCTGTTGGAGACAGGTGTTGAATGCACG
GCCTCCAACGACCTGGGCAAAAACACCAGCATCCTCTTCCTGGAGCTGGTCAATTTAACC
ACCCTCACACCAGACTCCAACACAACCACTGGCCTCAGCACTTCCACTGCCAGTCCTCAT
ACCAGAGCCAACAGCACCTCCACAGAGAGAAAGCTGCCGGAGCCGGAGAGCCGGGGCGTG
GTCATCGTGGCTGTGATTGTGTGCATCCTGGTCCTGGCGGTGCTGGGCGCTGTCCTCTAT
TTCCTCTATAAGAAGGGCAAGCTGCCGTGCAGGCGCTCAGGGAAGCAGGAGATCACGCTG
CCCCCGTCTCGTAAGAGCGAACTTGTAGTTGAAGTTAAGTCAGATAAGCTCCCAGAAGAG
ATGGGCCTCCTGCAGGGCAGCAGCGGTGACAAGAGGGCTCCGGGAGACCAGGGAGAGAAA
TACATCGATCTGAGGCATTAG

Clone variation with respect to NM_006500.2
1800 a=>g
>OriGene 5' read for NM_006500 unedited
TAACGTCAAATTTTGTATACGACTCCTATAGGCGGCACGCGAATTCGCACGAGAGGGGCT
TCCCAGGCTGGTCTGCGCCTTCTTGCTCGCCGCCTGCTGCTGCTGTCCTCGCGTCGCGGG
TGTGCCCGGAGAGGCTGAGCAGCCTGCGCCTGAGCTGGTGGAGGTGGAAGTGGGCAGCAC
AGCCCTTCTGAAGTGCGGCCTCTCCCAGTCCCAAGGCAACCTCAGCCATGTCGACTGGTT
TTCTGTCCACAAGGAGAAGCGGACGCTCATCTTCCGTGTGCGCCAGGGCCAGGGCCAGAG
CGAACCTGGGGAGTACGAGCAGCGGCTCAGCCTCCAGGACAGAGGGGCTACTCTGGCCCT
GACTCAAGTCACCCCCCAAGACGAGCGCATCTTCTTGTGCCAGGGCAAGCGCCCTCGGTC
CCAGGAGTACCGCATCCAGCTCCGCGTCTACAAAGCTCCGGAGGAGCCAAACATCCAGGT
CAACCCCCTGGGCATCCCTGTGAACAGTAAGGAGCCTGAGGAGGTCGCTACCTGTGTAGG
GAGGAACGGGTACCCCATTCCTCAAGTCATCTGGTACAAGAATGGCCGGCCTCTGAAGGA
GGAGAAGAACCGGGTCCACATTCAGTCGTCCCAGACTGTGGAGTCGAGTGGTTTGTACAC
CTTGCAGAGTATTCTGAAGGCACAGCTGGTTAAAGAAGACAAAGATGCCCAGTTTTACTG
TGAGCTCAACTACCGGCTGCCCAGTGGGAACCACATGAAGGAGTCCAGGGAAGTCACCGT
CCCTGTTTTCTNACCCGACAGATAAGTGTGGCTGGAAGTGGAGCCCGTGGGAATGCTGAA
NGAGGGGACCGCGTGGAATCATGTGTTTGGGCTGATGGCACCCTNCACCACACTTCAGCA
TCAGCAAGCATGATCCCAGCACC
>OriGene 3' read for NM_006500 unedited
CTATGTAACGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTGATTTCTGGGG
CAATTAAGCTTTATTTTTCATATATATATATATTCTTCATATATATATATACATACATAT
ATAAAGGAAACAATTTGCAAATTTACACACCTGACAAAACCATATATACACACATATGTA
TGCATACACACAGACAGACACACACACCCGAAGCTCTAGCCAGGCCCGTTTTCCATCCCT
AAGTACCATTCTCTCATTTGGGCCCTTCTAAGGTTGGGGCCCTGAGCTTGGTTTGTAGAA
GTTTGGTGCTAATATAACCATAGCTTTAATCCCCATGAAGGACAGTGTAGACCTCATCTT
TGTCTGCTCCCCGCTGCCTTTCAGTTTTACGTGATCCATCAAGAGGGCTATGGGAGCCAA
GTGAACACGGGGGATTGAGGCTAATTCACCTGAACTCGAAAACAGCGCCCAGCTTCCTCA
CTGCAAGCACGTGTCTTTTCTTTTTTTTTTCTCGAGACGGAGTCTCGCTGTGTTGCCCAA
GCTGGAGTGCAGTGGCACGGTCTCGGCTCACTGCAAGCTCCACCTCCTGGATTCATACCA
TTCTCCTGCTTCAAGCCTTCGAGTAGCTGGGACTATATGTGCCAACCACTACGCCTAGCT
AACTCCTTCTAGATTTTTAATACACACAGGCTTTCACCGGGTTAACCACGATGGACTTGT
CCTGAATTTGTGATCCGCCCGCCTCGGCCCACAAAAGGCCGGGATTACAGGTGTGAGACA
CCACACCTGGCCCCGGAACGTATTTTTAAAGAAGACACCAATTCTTGGTTTTGCACAAGA
AAATAGTCAAGGATATTTGTACAGCACAATGAGGGTGGGGCCCACCCTGCAACTGCTATG
GATGCTTCGGGGGGTTCTGCAGCGGNGGTACAAACCACTAAACTTATCGGCCCCGCACCG
GGAACCTCTTAACGTGGCCCGGGAG
Restriction Sites NotI-NotI     
ACCN NM_006500
Insert Size 3460 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006500.2, NP_006491.2
RefSeq Size 3332 bp
RefSeq ORF 1941 bp
Locus ID 4162
UniProt ID P43121
Domains ig, IGc2, IG
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Gene Summary Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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