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AMPK alpha 2 (PRKAA2) (NM_006252) Human Untagged Clone
CAT＃: SC116247
PRKAA2 (untagged)-Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)
CNY 6,176.00
| Cited in 3 publications. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMPK; AMPK2; AMPKa2; PRKAA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_006252 edited
GGCGGCCGCGCAATTCGCACGAGGCCGCGCGCGCCGAAGATGGCTGAGAAGCAGAAGCAC GACGGGCGGGTGAAGATCGGACACTACGTGCTGGGCGACACGCTGGGCGTCGGCACCTTC GGCAAAGTGAAGATTGGAGAACATCAATTAACAGGCCATAAAGTGGCAGTTAAAATCTTA AATAGACAGAAGATTCGCAGTTTAGATGTTGTTGGAAAAATAAAACGAGAAATTCAAAAT CTAAAACTCTTTCGTCATCCTCATATTATCAAACTATACCAGGTGATCAGCACTCCAACA GATTTTTTTATGGTAATGGAATATGTGTCTGGAGGTGAATTATTTGACTACATCTGTAAG CATGGACGGGTTGAAGAGATGGAAGCCAGGCGGCTCTTTCAGCAGATTCTGTCTGCTGTG GATTACTGTCATAGGCATATGGTTGTTCATCGAGACCTGAAACCAGAGAATGTCCTGTTG GATGCACACATGAATGCCAAGATAGCCGATTTCGGATTATCTAATATGATGTCAGATGGT GAATTTCTGAGAACTAGTTGCGGATCTCCAAATTATGCAGCACCTGAAGTCATCTCAGGC AGATTGTATGCAGGTCCTGAAGTTGATATCTGGAGCTGTGGTGTTATCTTGTATGCTCTT CTTTGTGGCACCCTCCCATTTGATGATGAGCATGTACCTACGTTATTTAAGAAGATCCGA GGGGGTGTCTTTTATATCCCAGAATATCTCAATCGTTCTGTCGCCACTCTCCTGATGCAT ATGCTGCAGGTTGACCCACTGAAACGAGCAACTATCAAAGACATAAGAGAGCATGAATGG TTTAAACAAGATTTGCCCAGTTACTTATTTCCTGAAGACCCTTCCTATGATGCTAACGTC ATTGATGATGAGGCTGTGAAAGAAGTGTGTGAAAAATTTGAATGTACAGAATCAGAAGTA ATGAACAGTTTATATAGTGGTGACCCTCAAGACCAGCTTGCAGTGGCTTATCATCTTATC ATTGACAATCGGAGAATAATGAACCAAGCCAGTGAGTTCTACCTCGCCTCTAGTCCTCCA TCTGGTTCTTTTATGGATGATAGTGCCATGCATATTCCCCCAGGCCTGAAACCTCATCCA GAAAGGATGCCACCTCTTATAGCAGACAGCCCCAAAGCAAGATGTCCATTGGATGCACTG AATACGACTAAGCCCAAATCTTTAGCTGTGAAAAAAGCCAAGTGGCATCTTGGAATCCGA AGTCAGAGCAAACCGTATGACATTATGGCTGAAGTTTACCGAGCTATGAAGCAGCTGGAT TTTGAATGGAAGGTAGTGAATGCATACCATCTTCGTGTAAGAAGAAAAAATCCAGTGACT GGCAATTACGTGAAAATGAGCTTACAACTTTACCTGGTTGATAACAGGAGCTATCTTTTG GACTTTAAAAGCATTGATGATGAAGTAGTGGAGCAGAGATCTGGTTCCTCAACACCTCAG CGTTCCTGTTCTGCTGCTGGCTTACACAGACCAAGATCAAGTTTTGATTCCACAACTGCA GAGAGCCATTCACTTTCTGGCTCTCTCACTGGCTCTTTGACCGGAAGCACATTGTCTTCA GTTTCACCTCGCCTGGGCAGTCACACCATGGATTTTTTTGAAATGTGTGCCAGTCTGATT ACTACTTTAGCCCGTTGATCTGTCTCTAGTTTCTTTCTGTTATTGCACTATGAAAATCAG TTATATTCTTTAAATTTTTATCTTACTTTTGGATAATATCCACTGCAATACTAATTGAGA AACATGAATTATTTCCAGGGGCACACAATGCTATTGAAATTACTGAAAACAAAATATCTG ACATCTTATTTACTTGTAGAAATCTGTAATTCTATTGTGCCTATGATAAATTCACATAGG CAATATCTTTAATAGGTTAATATCAATGAAGATTTTTAATTACAATAATGAGTTCACTAC AGACGATTAACACACCACACTGGCGAACCATCTCAATGTAAGGGTGGTTTGGCAACACCT CCTTGCTTTGCTGTTTGGTGTAGGTAAATCTAGTTTACTTCCTAAATTTCAGTAGGCTTT ATGCTGTGTTTATGCCCCCAATTTATTTTAACAAAAGAAGATTAAAAAGTAAAAGAACCA CGAGTAAGATATTATTTAAATGTTGAAATCTTAAAAACCTGCCTCCAAGATTTCAGAAGC CAAGTTTTTCTAACAGTATTTGTACAAATACTGCCTAGTGTATTCAACAGAAGGACTGTG GGTCATGTAACAGGTAACCACAATTTTC >OriGene 5' read for NM_006252 unedited
NGCTTGTACCGCTAAAACGACTCATATAGGCGGCCGCGCAATTCGCACGAGGCCGCGCGC GCCGAAGAGGCTGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGACACTACGTGCTG GGCGACACGCTGGGCGTCGGCACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTAACA GGCCATAAAGTGGCAGTTAAAATCTTAAATAGACAGAAGATTCGCAGTTTAGATGTTGTT GGAAAAATAAAACGAGAAATTCAAAATCTAAAACTCTTTCGTCATCCTCATATTATCAAA CTATACCAGGTGATCAGCACTCCAACAGATTTTTTTATGGTAATGGAATATGTGTCTGGA GGTGAATTATTTGACTACATCTGTAAGCATGGACGGGTTGAAGAGATGGAAGCCAGGCGG CTCTTTCAGCAGATTCTGTCTGCTGTGGATTACTGTCATAGGCATATGGTTGTTCATCGA GACCTGAAACCAGAGAATGTCCTGTTGGATGCACACATGAATGCCAAGATAGCCGATTTC GGATTATCTAATATGATGTCAGATGGTGAATTTCTGAGAACTAGTTGCGGATCTCCAAAT TATGCAGCACCTGAAGTCATCTCAGGCAGATTGTATGCAGGTCCTGAAGTTGATATCTGG AGCTGTGGTGTTATCTTGTATGCTCTTCTTTGTGGCACCCTCCCATTTGATGATGAGCAT GTACCTACGTTATTTAAGAAGATCCGAGGGGGTGTCTTTTATATCCCAGAATATCTCAAT CGTTCTGTCGCCACTCTCCTGATGCATATGCTGCAAGTTGACCCACTGAAACGAGCGACT ATCAAAGACATAAGAGAGCATGAAATGGGTTAANACAAGATTTGCCCAGTTACTTATTTT CTGGAGACCCTTCCTATGATGCTTACGTCAATGATGAT >OriGene 3' read for NM_006252 unedited
NAATACTTGNACCGCGCCGCATTCTANATCGAGTTTTTTTCTTTTTTTTTTTTTGCTTAA AACACTGGTGTGTGAATATATATATTTATTTATTTAAGATAAAAATTTTTTCTTGATGTT CAAAGCTTCCCTGCCCTATCAGGTTCAACAGTAAAGGTCATAGCCAGCCCTTAGGGCCAG AACAGTGTTTTCAAACTTGGACTAGAAAAGATTCAAAGCTATATCAATCAACCACTCAGG ATCAAACAATGAACAAAGACAATAGTGACTAGGTGAGGTGAGGTGAAAGGAGTGGCTTCC AATGAAGAATTAGCATTATGAGCTGCACCAGGACCCTAACACCTAACACAGAAAATAAAG ATCAAAGATAAGGATTCTCATTTAAGGGACTGTTGATACTTTGCTTTGTTTCATTTAACT ATTTTATCACTGTTGGTGAAATGCAAAAATAAATAGAGGCAAAAAGAATTTGAGAAAGGT AGATTTTGAAGACATTTTTTAAATGACGTAAGTTTATGTAAAAGACAAAAGTTGTTGCAA TTCCAGATGTTCCAATATTAATTTGAAACTTGAAGGCTCTTAAGATAAATATATTATTCA GGGAAATCTCAAGATAAAAATCCTGAGTTAGTTACAGCTGTTTTTAAGAAACCTGAAAAT TGTGGTTACCTGTTACATGACCACAGTCCTTCTGTTGAATACACTAGGCAGTATTTGTAC AAATACTGTTAGAAAAACTTGGCTTCTGAAATCTTGGAAGCAGGTTTTTTAGATTTCAAC ATTTTAATAATATCTTACTCGTGGTTCTTTTACCTTTTAAACTTCCTTTGTTAAATAAAT TGGGGGCATAAACCCCGCATAAAGCCCACTGAAATTTAGGAAGTTAACTAGATTTACCTA CNCCCAACACCAAACAGGGAGGTGTTGCACAACCACCTTACAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006252 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006252.2, NP_006243.2 |
| RefSeq Size | 2435 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 5563 |
| UniProt ID | P54646 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy |
| Gene Summary | The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
AMPK regulates immunometabolism in sepsis.
,null,
Brain, behavior, and immunity
,PubMed ID 29109024
[PRKAA2]
|
|
Activation of AMP-activated protein kinase a2 by nicotine instigates formation of abdominal aortic aneurysms in mice in vivo
,Shuangxi Wang, Cheng Zhang, Miao Zhang, Bin Liang, Huaiping Zhu,,
Nature Medicine 18, 902-910 doi:10.1038/nm.2711
[PRKAA2]
|
|
The energy sensor AMP-activated protein kinase directly regulates the mammalian FOXO3 transcription factor
,Eric L Greer, Philip R Oskoui, Max R Banko, Jay M Maniar, Melanie P Gygi, Steven P Gygi, and Anne Brunet,
J Biol Chem. 2007 Oct 12;282(41):30107-19.
[PRKAA2]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210226 | PRKAA2 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2) |
CNY 6,168.00 |
|
| RC210226L1 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), Myc-DDK-tagged |
CNY 8,568.00 |
|
| RC210226L2 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), mGFP tagged |
CNY 8,568.00 |
|
| RC210226L3 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210226L4 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), mGFP tagged |
CNY 8,568.00 |
|
| RG210226 | PRKAA2 (tGFP-tagged) - Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2) |
CNY 7,768.00 |
|
| SC323523 | PRKAA2 (untagged)-Kinase deficient mutant (K45M) of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2) |
CNY 6,176.00 |
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