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AMPK alpha 2 (PRKAA2) (NM_006252) Human Untagged Clone
CAT＃: SC323523
PRKAA2 (untagged)-Kinase deficient mutant (K45M) of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)
CNY 6,176.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMPK; AMPK2; AMPKa2; PRKAA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323523 sequence for NM_006252 edited (data generated by NextGen Sequencing)
ATGGCTGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGACACTACGTGCTGGGCGAC ACGCTGGGCGTCGGCACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTAACAGGCCAT AAAGTGGCAGTTATGATCTTAAATAGACAGAAGATTCGCAGTTTAGATGTTGTTGGAAAA ATAAAACGAGAAATTCAAAATCTAAAACTCTTTCGTCATCCTCATATTATCAAACTATAC CAGGTGATCAGCACTCCAACAGATTTTTTTATGGTAATGGAATATGTGTCTGGAGGTGAA TTATTTGACTACATCTGTAAGCATGGACGGGTTGAAGAGATGGAAGCCAGGCGGCTCTTT CAGCAGATTCTGTCTGCTGTGGATTACTGTCATAGGCATATGGTTGTTCATCGAGACCTG AAACCAGAGAATGTCCTGTTGGATGCACACATGAATGCCAAGATAGCCGATTTCGGATTA TCTAATATGATGTCAGATGGTGAATTTCTGAGAACTAGTTGCGGATCTCCAAATTATGCA GCACCTGAAGTCATCTCAGGCAGATTGTATGCAGGTCCTGAAGTTGATATCTGGAGCTGT GGTGTTATCTTGTATGCTCTTCTTTGTGGCACCCTCCCATTTGATGATGAGCATGTACCT ACGTTATTTAAGAAGATCCGAGGGGGTGTCTTTTATATCCCAGAATATCTCAATCGTTCT GTCGCCACTCTCCTGATGCATATGCTGCAGGTTGACCCACTGAAACGAGCAACTATCAAA GACATAAGAGAGCATGAATGGTTTAAACAAGATTTGCCCAGTTACTTATTTCCTGAAGAC CCTTCCTATGATGCTAACGTCATTGATGATGAGGCTGTGAAAGAAGTGTGTGAAAAATTT GAATGTACAGAATCAGAAGTAATGAACAGTTTATATAGTGGTGACCCTCAAGACCAGCTT GCAGTGGCTTATCATCTTATCATTGACAATCGGAGAATAATGAACCAAGCCAGTGAGTTC TACCTCGCCTCTAGTCCTCCATCTGGTTCTTTTATGGATGATAGTGCCATGCATATTCCC CCAGGCCTGAAACCTCATCCAGAAAGGATGCCACCTCTTATAGCAGACAGCCCCAAAGCA AGATGTCCATTGGATGCACTGAATACGACTAAGCCCAAATCTTTAGCTGTGAAAAAAGCC AAGTGGCATCTTGGAATCCGAAGTCAGAGCAAACCGTATGACATTATGGCTGAAGTTTAC CGAGCTATGAAGCAGCTGGATTTTGAATGGAAGGTAGTGAATGCATACCATCTTCGTGTA AGAAGAAAAAATCCAGTGACTGGCAATTACGTGAAAATGAGCTTACAACTTTACCTGGTT GATAACAGGAGCTATCTTTTGGACTTTAAAAGCATTGATGATGAAGTAGTGGAGCAGAGA TCTGGTTCCTCAACACCTCAGCGTTCCTGTTCTGCTGCTGGCTTACACAGACCAAGATCA AGTTTTGATTCCACAACTGCAGAGAGCCATTCACTTTCTGGCTCTCTCACTGGCTCTTTG ACCGGAAGCACATTGTCTTCAGTTTCACCTCGCCTGGGCAGTCACACCATGGATTTTTTT GAAATGTGTGCCAGTCTGATTACTACTTTAGCCCGTTGA Clone variation with respect to NM_006252.3 134 a=>t;135 a=>g >OriGene 5' read for mutant NM_006252 unedited
ACGCCCGAACCGCAACGGGCGGAGGCGCGCACGGAGGGAGGACAACACAAGAGAGCCCGACAAGCGAACC GACAGAAAAACGAAAAACGACACACCAAAGGGCGGCCGCGAACACGGCACGAGGCCGCGCGCGCCGAAGA CGGCTGAGAAGCAGAAGCACGACGGGCGGGTGAAGACCGGACACTACGAGCTGGGCGACACGCTGGGCGT CGGCACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTTAACAGGCCATAAAGTGGCAGTTATGATTCT TAAATAGACAGAAGATTCGCAGTTTTAGATGTTTGTTGGAAAAATAAAACGAGAAAATTCAAAATCTAAA ACTCTTTCGTCATCCTCATATTTATCAACCTATTACCAGGGTGGATCCAGCACTTCCAACAAGATATTTT TTTATTGGTTATGGGGAATATGTTGGTTCGGAGAGGTGGAAATATATTGGAACTACATCTCTGTAAGGCC TGGGACACGGTTTTGAAAGAAAATGGGAACCAAGCCCGGCTTTTTTCGGAGAGATTCGGTTCGCGGTGGG GATTTACGGGCATTAGAGCATATATGGGTTGGTTACTTCAGAACACTTGAAACCCAAAAAATGTCCTTTT GGAAACGCACATGAAAGTGCCAGAAATGCCAGTTTTCAGTTTATCAAATTATAGTGAGAAAGGGAAATTT TGAGAAACCGTTGTCGAGATTCTCCAATTTGGGCCACCTGTGAATATCTTCTGCGGCAATGTGTGCCCGT GCTCCAAATTGATATATTGGGGGAGCGGGTGGGTATCATGTGTGACTCCCTCTCTCGTGGGGGCCCTCCC TCACTTTGATGGATGAAGCACGTGTGCTACCGTCATTTTATGAAGATCCACCGGGTGTCTTTCTTATATC TCCGCAAATCCCATCGTCTGTCGCACTCTCTCGAGCATATACTGCAGTTGTCCACTGAGCAGCGACCATC TTAGACTATAGACGCATGTGTAACAGATGCGGTACTATTCGAGAACATCCTCGAGGCTAGCG >SC323523 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYTTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCGCGCGCCGAAGATGGC TGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGACACTACGTGCTGGGCGACACGCTGGGCGTCGGC ACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTAACAGGCCAT |
| Restriction Sites | Please inquire |
| ACCN | NM_006252 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006252.2, NP_006243.2 |
| RefSeq Size | 2435 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 5563 |
| UniProt ID | P54646 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy |
| Gene Summary | The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210226 | PRKAA2 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2) |
CNY 6,168.00 |
|
| RC210226L1 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), Myc-DDK-tagged |
CNY 8,568.00 |
|
| RC210226L2 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), mGFP tagged |
CNY 8,568.00 |
|
| RC210226L3 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210226L4 | Lenti ORF clone of Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), mGFP tagged |
CNY 8,568.00 |
|
| RG210226 | PRKAA2 (tGFP-tagged) - Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2) |
CNY 7,768.00 |
|
| SC116247 | PRKAA2 (untagged)-Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2) |
CNY 6,176.00 |
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