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G3BP (G3BP1) (NM_005754) Human Untagged Clone
CAT＃: SC116551
G3BP1 (untagged)-Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1
CNY 5,488.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | G3BP; HDH-VIII |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116551 sequence for NM_005754 edited (data generated by NextGen Sequencing)
ATGGTGATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAATTTGTGAGACAGTATTAC ACACTGCTGAACCAGGCCCCAGACATGCTGCATAGATTTTANNNNNNNNNCTCTTCTTAT GTCCATGGGGGATTGGATTCAAATGGAAAGCCAGCAGATGCAGTCTACGGACAGAAAGAA ATCCACAGGAAAGTGATGTCACAAAACTTCACCAACTGCCACACCAAGATTCGCCATGTT GATGCTCATGCCACGCTAAATGATGGTGTGGTAGTCCAGGTGATGGGGCTTCTCTCTAAC AACAACCAGGCTTTGAGGAGATTCATGCAAACGTTTGTCCTTGCTCCTGAGGGGTCTGTT GCAAATAAATTCTATGTTCACAATGATATCTTCAGATACCAAGATGAGGTCTTTGGTGGG TTTGTCACTGAGCCTCAGGAGGAGTCTGAAGAAGAAGTAGAGGAACCTGAAGAAAGACAG CAAACACCTGAGGTGGTACCTGATGATTCTGGAACTTTCTATGATCAGGCAGTTGTCAGT AATGACATGGAAGAACATTTAGAGGAGCCTGTTGCTGAACCAGAGCCTGATCCTGAACCA GAACCAGAACAAGAACCTGTATCTGAAATCCAAGAGGAAAAGCCTGAGCCAGTATTAGAA GAAACTGCCCCTGAGGATGCTCAGAAGAGTTCTTCTCCAGCACCTGCAGACATAGCTCAG ACAGTACAGGAAGACTTGAGGACATTTTCTTGGGCATCTGTGACCAGTAAGAATCTTCCA CCCAGTGGAGCTGTTCCAGTTACTGGGATACCACCTCATGTTGTTAAAGTACCAGCTTCA CAGCCCCGTCCAGAGTCTAAGCCTGAATCTCAGATTCCACCACAAAGACCTCAGCGGGAT CAAAGAGTGCGAGAACAACGAATAAATATTCCTCCCCAAAGGGGACCCAGACCAATCCGT GAGGCTGGTGAGCAAGGTGACATTGAACCCCGAAGAATGGTGAGACACCCTGACAGTCAC CAACTCTTCATTGGCAACCTGCCTCATGAAGTGGACAAATCAGAGCTTAAAGATTTCTTT CAAAGTTATGGAAACGTGGTGGAGTTGCGCATTAACAGTGGTGGGAAATTACCCAATTTT GGTTTTGTTGTGTTTGATGATTCTGAGCCTGTTCAGAAAGTCCTTAGCAACAGGCCCATC ATGTTCAGAGGTGAGGTCCGTCTGAATGTCGAAGAGAAGAAGACTCGAGCTGCCAGGGAA GGCGACCGACGAGATAATCGCCTTCGGGGACCTGGAGGCCCTCGAGGTGGGCTGGGTGGT GGAATGAGAGGCCCTCCCCGTGGAGGCATGGTGCAGAAACCAGGATTTGGAGTGGGAAGG GGGCTTGCGCCACGGCAGTGA Clone variation with respect to NM_005754.2 102 t=>n;103 g=>n;104 g=>n;105 a=>n;106 a=>n;107 a=>n;108 g=>n;109 a=>n;110 a=>n >OriGene 5' read for NM_005754 unedited
GTGCAAATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGTGCGTG AGGGGTTTGTACTATCCTCGGTGCTGTGGTGCAGAGCTAGTTCCTCTCCAGCTCAGCCGC GTAGGTTTGGACATATTTGACTCTTTTCCCCCCAGGTTGAATTGACCAAAGCAATGGTGA TGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAATTTGTGAGACAGTATTACACACTGC TGAACCAGGCCCCAGACATGCTGCATAGATTTTATGGAAAGAACTCTTCTTATGTCCATG GGGGATTGGATTCAAATGGAAAGCCAGCAGATGCAGTCTACGGACAGAAAGAAATCCACA GGAAAGTGATGTCACAAAACTTCACCAACTGCCACACCAAGATTCGCCATGTTGATGCTC ATGCCACGCTAAATGATGGTGTGGTAGTCCAGGTGATGGGGCTTCTCTCTAACAACAACC AGGCTTTGAGGAGATTCATGCAAACGTTTGTCCTTGCTCCTGAGGGGTCTGTTGCAAATA AATTCTATGTTCACAATGATATCTTCAGATACCAAGATGAGGTCTTTGGTGGGTTTGTCA CTGAGCCTCAGGAGGAGTCTGAAGAAGAAGTAGAGGAACCTGAAGAAAGACAGCAAACAC CTGAGGTGGTACCTGATGATTCTGGAACTTTCTATGATCANGCAGTTGTCAGTAATGACA TGGAAGAACATTTAGAGGAGCCTGTTGCTGAACCAGAGCCTGATTCTTGACCAGAACCAG ACCAGAACCTGTATCTGAAATCCCAGAGGAAAGCCTGAGCCATATTAGAAGAACTGCCCC TGTAGATGCTCAGAAGAGTTCTTCTCCAGCACTGCCGACTAGCTCAGCAGTACACGAAGA CTTGAGACATTTTTTTGGCATCTG >OriGene 3' read for NM_005754 unedited
CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTCCAGAATATTTTAATAAAAATT GGCCATTTCCAAGTAACACCCACTGCATGCCCATCAATAAAAGAGAACCATAACAAAATA GAAAATTTACCATATGGCTATGATTTTAAGAAAAAAAAAAATGCGGACACGAGCACATGA GCAAAGTCCAATATTAAAGTAACATTCCTTCTTACGGGCTATGATTAGGAATCTGATTAG ATACCAGAATAAAAATACAAATGCTGAACTATTTAGGTCAAAGAACCTCTATAGAAGCAT CTGTCTTCAAAGGCATTCCAGTTTGAGACTCAATTTTAGAATAAGGCATAACAAATATGT TAACAAAAGACATACACAAAGTGATGAAAGAGGGATTCTATAATTCCCCACATACTCTGA ACCTAATAAAACAAATCTAGATTTTTTTTTTAAACAATTGCCAAGACTGAAATCAAAGAT AAATAGAAGGCACAACAGTTTTGCTCTGGTTGTTATCTGGGTTTTGGGAATTTTTTTGCT TTTTGTTTTTTTACAAATACAAATTAAACATGAAAAAACTACTTCAAAAAAACTAACAGT TCAAGAAAAGCCTATCAGTTGCATTCTAGACATTTAAAACCTATTATCACAATTTAAATT TCAATGGTAAAAACAGTTAGGTTTTAGAATTGTTTTGTGATCCCTTATCAAAAAAAAATC TATTCCTTTNNATTCAGTTTTAAACATACCAGTGAGAATCAAATTTGTAAATTTTTCAAT GGCATTTCTCCACACAAAAGTAGTCATGACTCTCAAAGAAGAACATTTGGGGTTTTCCTG CCTCAAAAATTATCAAACCTGTATTCTTACAGGCTTTCAACACGTAAATATAAAAATTTC CATGGATGAACTACCAATGGCGTATATGCAAACTCCTGGGGGAATGCTGAAAAAAAAAAA AACACTCGTTTGCAAGAACAAGGATCAACTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005754 |
| Insert Size | 3030 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005754.2, NP_005745.1 |
| RefSeq Size | 2855 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 10146 |
| UniProt ID | Q13283 |
| Domains | RRM, NTF2 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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OGFOD1, a Novel Modulator of Eukaryotic Translation Initiation Factor 2{alpha} Phosphorylation and the Cellular Response to Stress
,Karen A. Wehner, Sylvia Schütz, and Peter Sarnow,
Mol. Cell. Biol., Apr 2010; 30: 2006 - 2016
[G3BP1]
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OGFOD1, a Novel Modulator of Eukaryotic Translation Initiation Factor 2? Phosphorylation and the Cellular Response to Stress ▿OGFOD1, a Novel Modulator of Eukaryotic Translation Initiation Factor 2? Phosphorylation and the Cellular Response to Stress ▿ †
,null,
Molecular and Cellular Biology
,PubMed ID 20154146
[G3BP1]
|
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OGFOD1: A Novel Modulator of eIF2{alpha} Phosphorylation and the Cellular Response to Stress
,Karen A. Wehner, Sylvia Schütz, and Peter Sarnow,
Mol. Cell. Biol., Feb 2010; 10.1128/MCB.01350-09
[G3BP1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202692 | G3BP1 (Myc-DDK-tagged)-Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1 |
CNY 3,656.00 |
|
| RC202692L1 | Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202692L2 | Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RC202692L3 | Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202692L4 | Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG202692 | G3BP1 (tGFP-tagged) - Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1 |
CNY 4,370.00 |
|
| SC321062 | G3BP1 (untagged)-Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1 |
CNY 5,488.00 |
