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ARHGEF7 (NM_003899) Human Untagged Clone
CAT＃: SC117668
ARHGEF7 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1
CNY 4,816.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117668 sequence for NM_003899 edited (data generated by NextGen Sequencing)
ATGACCGACAATAGCAACAATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACC AATGAGGACGAGCTTTCCTTCTCAAAAGGAGACGTCATCCATGTCACCCGTGTGGAAGAG GGAGGCTGGTGGGAGGGCACACTCAACGGCCGGACCGGCTGGTTCCCCAGCAACTACGTG CGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCTCCCAAATCAGGAACACTGAAGAGCCCT CCCAAAGGATTTGATACGACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAAT ATTTTAGAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTA CGGCCATTGCAGACCAGTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAAT CTAGAAGAAATATGTTCTTTCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAG TTGCCCGAAGCTCAGCAGAGAGTCGGAGGCTGCTTTTTAAACCTGATGCCACAGATGAAA ACCCTGTACCTCACGTATTGTGCCAATCACCCTTCTGCAGTGAATGTCCTCACGGAACAC AGTGAGGAGTTGGGGGAGTTCATGGAGACCAAAGGTGCCAGCAGCCCTGGGATTCTCGTG CTGACCACGGGCCTGAGCAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAAA GAGCTCGAGAGACACATGGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATG GCTGCCTTCAAAAACCTTTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAG CTGCAGATCCTGACGGAAGCCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTGGGC AACGTCACTTACATGTCCCAGGTCCTGATTCAGTGTGCCGGAAGTGAGGAAAAGAATGAA AGATATCTTCTACTCTTCCCAAATGTTTTGCTAATGTTGTCTGCCAGTCCTAGGATGAGT GGCTTTATCTATCAGGGAAAGCTTCCAACGACAGGAATGACAATCACAAAGCTTGAGGAC AGTGAAAATCATAGAAATGCATTTGAAATATCAGGGAGCATGATTGAGCGGATATTAGTG TCGTGCAACAACCAGCAGGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAG GTCACGTCTGTGGGAAACCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCC TCCCACCCGGTCACTCCGTCCAGCAAGCACGCAGACAGCAAGCCCGCGCCGCTGACGCCC GCCTACCACACGCTGCCCCACCCCTCCCACCACGGCACCCCGCACACCACCATCAACTGG GGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGCCTGAGCTGCCTGCGGCCCGCG CCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTAAGAGCCCTAAG ACCATGAAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTTC GCGTCCCGGAAAAGCACAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAA GCTTACTGCACCAGCGCCAAAACAAGGCAAACACTCAATTCAAGTTCACGCAAAGAATCT GCTCCACAAGTTTTGCTTCCAGAAGAAGAGAAAATTATAGTGGAAGAAACTAAAAGTAAT GGTCAGACAGTGATAGAAGAAAAGAGTCTTGTGGATACCGTATATGCATTAAAGGATGAA GTTCAAGAATTAAGACAGGACAACAAAAAGATGAAGAAATCTCTAGAGGAAGAACAGAGA GCCCGCAAAGACCTGGAGAAGCTGGTGAGGAAAGTCCTGAAGAACATGAATGATCCTGCC TGGGATGAGACCAATCTATAA Clone variation with respect to NM_003899.3 9 t=>c >OriGene 5' read for NM_003899 unedited
CGTTANCATTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGGCAAACCGT TACGTGGCTCATCACTCTGGGGGTGCTGGAGTCGCCCAAAAAAACCATCTCGGACCCGGA GGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTGGTCCTCTGCAGGCTGCTGGAGCGCCT GCTCCCCGGGACCATCGAGAAAGTCTACCCCGAGCCCCGGAGCGAGAGCGAGTGCCTGAG CAACATCCGCGAGTTCCTGCGCGGCTGCGGGGCTTCCCTGCGGCTGGAGACGTTTGATGC AAATGATTTGTATCAGGGGCAGAATTTTAACAAGGTCCTCAGTTCCTTAGTGACTCTAAA TAAAGTAACAGCAGACATCGGGCTGGGGAGTGACTCCGTGTGTGCCCGGCCCTCGTCTCA CCGCATAAAGTCTTTTGACTCCCTTGGATCACAGTCTTTGCACACTCGGACTTCAAAACT GTTCCAGGGCCAGTATCGGAGTTTGGACATGACCGACAATAGCAACAATCAACTGGTAGT AAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGACGAGCTTTCCTTCTCAAAGGAGAC GTCATCCATGTCACCCGTGTGGAATAGGAGGCTGTGGNGGAAGGGCACACTCAACGGCCG GACCGGCTGGTTCCCAGCAACTACGTGCGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCT CCCATATCATGACACTGAAGAGCCCTCCAAAGGATTTGATACGACTGCCATANNACAAAC ATTACAATGTGGTGCTACAGAATATTTTAGAAACAGATNATGAATATTCTAAAGAATTNC AGACTGTGCTTTTCACGTACCTACGGCCATTGCAGACANTGGAAAAAAGTTAAGTTCAGC ANACATTTCTNATTTAATGGNGAATNCTAGAAGAATTAATGCCCCTT >OriGene 3' read for NM_003899 unedited
TGCTCTGGACCGGCGGCCGCAATCTAGGGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTG ACATAAGAAAAAAATCAGTTTAATAATTACAACAGTGTAGCATCAACATTGTAGTAGTCA ACACTAGGACATTGTCTGTGACATTTAAATTAGGAGTTTCTCCACACTTGTCAACAGGCG CCCTGGGTGTCCCAGAGAAGTGAAGAAATGATTGGCATTAAGGAGTCCACACAACCAGGG CAGGCATTCAAGGTCCCAGACGCCCAGTGCATGTGGACAAGCACAGACGCCACTGAGTGT GAAGGAACGAGCTTCTGATGCTACATTCCAACATCGCTGAAAACTGAAAGTGCTTCACAT CTACAGGATAAGAAATGGCTTCATCTCCTCATCCATACAAGGTGAGAGCAAAGGAGTAAC CCTCGGGCAAATCGTGGCCCCTTTAAACGTTTATATCAAGTTCCCACCCCCTTGGGAGGT CACACGGTGAAACAGACACAGTTATATACAACAGGGCAGGTTTTTAAAAAGAGTTGCTCT CAGACGCATTTTTCCTGCTCCCTAAAAAGCCGAGGAAGATACTGGATCCACAGAAAGAAA AGGCAATGCCGTAACATGAGGCCCTCATGGCCGCACCGTCCAGGGGAAGGGCTGNTAAAA ACACAAGTATTCTTGTNGAATACTTCGATCTGAGCATTAAGGCAGGTCTGCAGGAGATCC GTCCTGGGGACTCCGACAGCAACGCTACCGGCTTCGAGAGGACAGTTAATGTCGCCCTCC CGCAGAGGGGCCGAGAGATCAAACAAGAGTTGTTCCTGAGTTAAAAACTGCTACACAGCA AACTCCCATAACTCAAGGTGAATGTTTTCCACTGGCATGCTTTACGCATACCAAAAATAA CTTGTAAACTCAGCCTACCAAAAGAAGTGTTGACTCTGCTGGGCCCCCTGATGAATAACC GCTAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003899 |
| Insert Size | 5130 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003899.2, NP_003890.1 |
| RefSeq Size | 5032 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 8874 |
| UniProt ID | Q14155 |
| Domains | RhoGEF, SH3, PH |
| Protein Pathways | Regulation of actin cytoskeleton |
| Gene Summary | This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains a different 3' coding region and 3' UTR, compared to variant 1. It initiates translation at a downstream start codon. Variants 1, 5, 6, 18, and 19 all encode isoform a (also known as p85Cool-1), which has a shorter N-terminus and a longer and distinct C-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Sorting Nexin 27 Protein Regulates Trafficking of a p21-activated Kinase (PAK) Interacting Exchange Factor (ß-Pix)-G Protein-coupled Receptor Kinase Interacting Protein (GIT) Complex via a PDZ Domain Interaction
,Julie L. Valdes, Jingrong Tang, Mark I. McDermott, Jean-Cheng Kuo, Seth P. Zimmerman, Stephen M. Wincovitch, Clare M. Waterman, Sharon L. Milgram, and Martin P. Playford,
J. Biol. Chem., Nov 2011; 286: 39403 - 39416.
[ARHGEF7]
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Sorting Nexin 27 Protein Regulates Trafficking of a p21-activated Kinase (PAK) Interacting Exchange Factor (?-Pix)-G Protein-coupled Receptor Kinase Interacting Protein (GIT) Complex via a PDZ Domain Interaction *
,null,
The Journal of Biological Chemistry
,PubMed ID 21926430
[ARHGEF7]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218946 | ARHGEF7 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1 |
CNY 4,808.00 |
|
| RC218946L1 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, Myc-DDK-tagged |
CNY 7,208.00 |
|
| RC218946L2 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC218946L3 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC218946L4 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG218946 | ARHGEF7 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1 |
CNY 6,408.00 |
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