United States
Germany
Japan
United Kingdom
China
EIF4A1 (NM_001416) Human Untagged Clone
CAT＃: SC119268
EIF4A1 (untagged)-Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001416, the custom clone sequence may differ by one or more nucleotides
ATGTCTGCGAGCCAGGATTCCCGATCCAGAGACAATGGCCCCGATGGGATGGAGCCCGAAGGCGTCATCG AGAGTAACTGGAATGAGATTGTTGACAGCTTTGATGACATGAACCTCTCGGAGTCCCTTCTCCGTGGCAT CTACGCGTATGGTTTTGAGAAGCCCTCTGCCATCCAGCAGCGAGCCATTCTACCTTGTATCAAGGGTTAT GATGTGATTGCTCAAGCCCAATCTGGGACTGGGAAAACGGCCACATTTGCCATATCGATTCTGCAGCAGA TTGAATTAGATCTAAAAGCCACCCAGGCCTTGGTCCTAGCACCCACTCGAGAATTGGCTCAGCAGATACA GAAGGTGGTCATGGCACTAGGAGACTACATGGGCGCCTCCTGTCACGCCTGTATCGGGGGCACCAACGTG CGTGCTGAGGTGCAGAAACTGCAGATGGAAGCTCCCCACATCATCGTGGGTACCCCTGGCCGTGTGTTTG ATATGCTTAACCGGAGATACCTGTCCCCCAAATACATCAAGATGTTTGTACTGGATGAAGCTGACGAAAT GTTAAGCCGTGGATTCAAGGACCAGATCTATGACATATTCCAAAAGCTCAACAGCAACACCCAGGTAGTT TTGCTGTCAGCCACAATGCCTTCTGATGTGCTTGAGGTGACCAAGAAGTTCATGAGGGACCCCATTCGGA TTCTTGTCAAGAAGGAAGAGTTGACCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGA GTGGAAGCTGGACACACTATGTGACTTGTATGAAACCCTGACCATCACCCAGGCAGTCATCTTCATCAAC ACCCGGAGGAAGGTGGACTGGCTCACCGAGAAGATGCATGCTCGAGATTTCACTGTATCCGCCATGCATG GAGATATGGACCAAAAGGAACGAGACGTGATTATGAGGGAGTTTCGTTCTGGCTCTAGCAGAGTTTTGAT TACCACTGACCTGCTGGCCAGAGGCATTGATGTGCAGCAGGTTTCTTTAGTCATCAACTATGACCTTCCC ACCAACAGGGAAAACTATATCCACAGAATCGGTCGAGGTGGACGGTTTGGCCGTAAAGGTGTGGCTATTA ACATGGTGACAGAAGAAGACAAGAGGACTCTTCGAGACATTGAGACCTTCTACAACACCTCCATTGAGGA AATGCCCCTCAATGTTGCTGACCTCATCTGA >OriGene 5' read for NM_001416 unedited
GATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGGATCATGTCTGCGA GCCAGGATTCCCGATCCAGAGACATGGCCCCGATGGGATGGAGCCCGAAGGCGTCATCGA GAGTAACTGGAATGAGATTGTTGACAGCTTTGATGACATGAACCTCTCGGAGTCCCTTCT CCGTGGCATCTACGCCTATGGTTTTGAGAAGCCCTCTGCCATCCAGCAGCGAGCCATTCT ACCTTGTATCAAGGGTTATGATGTGATTGCTCAAGCCCAATCTGGGACTGGGAAAACGGC CACATTTGCCATATCAATTCTGCAGCAGATTGAATTAGATCTAAAAGCCACCCAGGCCTT GGTCCTAGCACCCACTCGAGAATTGGCTCAGCAGATACAGAAGGTGGTCATGGCACTAGG AGACTACATGGGCGCCTCCTGTCACGCCTGTATCGGGGGCACCAACGTGCGTGCTGAGGT GCAGAAACTGCAGATGGAAGCTCCCCACATCATCGTGGGTACCCCTGGCCGTGTGTTTGA TATGCTTAACCGGNAGATACCTGTCCCCCAAATACATCAAGATGTNTTGTACTGGATGAA GCTGACGAAATGTTAAGCCGTGGATTCAAGGACCAGATCTATGACATATTCCAAAAGCTC AACAGCAACACCCAGGNTAGTTNTTGCTGTCAGCCACAATGCCTTTCTGATGTGCTTGAG GTGCCCAAGAAGTTCATGANGGGACCCCATTCNGNATTCTTGTCAGAAGGNAAGAGTTGA CCCTGGAGGGTATNCCGCCAGTTCTCCATCAACGTGAACGAANAGANTGGGAACTGGACC CACTATGTGACTTGAATGAACCCTGACCATACCCC >OriGene 3' read for NM_001416 unedited
CCCTTGANGCCGGCGACCTCGGGGTTGAGACCTACCCCAAATAACAGGTGTCTAAAAGTT CCGGTTCCTTCTTTTGCCTCAAAAATGACATTTATTCAAAGAAAAAAATGACAAGATGTC CATCCCTTGGCTCCCTTCCCTCCCCCCTCCTGCTGCTCCTCAGCCCCCAGAGATTGAGCC CTGGCTGGGGCTGGGTGGCAGGACAGCCCCTCAGATGATGTCAGCAACATTGAGGGGCAT TTCCTCAATGGAGGTGTTGTAGAAGGTCTCAATGTCTCGAAGAGTCCTCTTGTCTTCTTC TGTCACCATGTTAATAGCCACACCTTTACGGCCAAACCGTCCACCTCGACCGATTCTGTG GATATAGTTTTCCCTGTTGGTGGGAAGGTCATAGTTGATGACTAAAGAAACCTGCTGCAC ATCAATGCCTCTGGCCAGCAGGTCAGTGGTAATCAAAACTCTGCTAGAGCCAGAACGAAA CTCCCTCATAATCACGTCTCGTTCCTTTTGGTCCATATCTCCATGCATGGCGGATACAGT GAAATCTCGAGCATGCATCTTCTCGGTGAGCCAGTCCACCTTCCTCCGGGTGTTGATGAA GATGACTGCCTGGGTGATGGTCAGGGTTTCATACAAGTCACATAGTGTGTCCAGCTTCCA CTCCTCTCGTTCCACGTTGATGTAGAACTGGCGGATACCCTCCAGGGTCAACTCTTCCTT CTTGACAAGAATCCGAATGGGGTCCCTCATGAACTTCTTGGTCACCTCAAGCACATCAGA AGGCATTGTGGCTGACAGCAAAACTACCTGAGTGTTGCTGNTGAGCTTTTGAATATGTCA TAGATCTGGGTCCTTGATCCACGGNCTTACATTTCGTCAGCTTCATCCAGTACAAACATC TTGATGTATTTGGGGGACAAGTATCTCCAGTTAAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001416 |
| Insert Size | 1900 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001416.1, NP_001407.1 |
| RefSeq Size | 1894 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 1973 |
| UniProt ID | P60842 |
| Domains | DEAD, helicase_C |
| Gene Summary | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203298 | EIF4A1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1 |
CNY 5,488.00 |
|
| RC203298L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203298L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC203298L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203298L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG203298 | EIF4A1 (tGFP-tagged) - Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1 |
CNY 7,088.00 |
|
| SC321083 | EIF4A1 (untagged)-Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1 |
CNY 5,488.00 |
