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CYBB (NM_000397) Human Untagged Clone
CAT＃: SC122091
CYBB (untagged)-Human cytochrome b-245, beta polypeptide (CYBB)
CNY 4,256.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMCBX2; CGD; CGDX; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC122091 sequence for NM_000397 edited (data generated by NextGen Sequencing)
ATGGGGAACTGGGCTGTGAATGAGGGGCTCTCCATTTTTGTCATTCTGGTTTGGCTGGGG TTGAACGTCTTCCTCTTTGTCTGGTATTACCGGGTTTATGATATTCCACCTAAGTTCTTT TACACAAGAAAACTTCTTGGGTCAGCACTGGCACTGGCCAGGGCCCCTGCAGCCTGCCTG AATTTCAACTGCATGCTGATTCTCTTGCCAGTCTGTCGAAATCTGCTGTCCTTCCTCAGG GGTTCCAGTGCGTGCTGCTCAACAAGAGTTCGAAGACAACTGGACAGGAATCTCACCTTT CATAAAATGGTGGCATGGATGATTGCACTTCACTCTGCGATTCACACCATTGCACATCTA TTTAATGTGGAATGGTGTGTGAATGCCCGAGTCAATAATTCTGATCCTTATTCAGTAGCA CTCTCTGAACTTGGAGACAGGCAAAATGAAAGTTATCTCAATTTTGCTCGAAAGAGAATA AAGAACCCTGAAGGAGGCCTGTACCTGGCTGTGACCCTGTTGGCAGGCATCACTGGAGTT GTCATCACGCTGTGCCTCATATTAATTATCACTTCCTCCACCAAAACCATCCGGAGGTCT TACTTTGAAGTCTTTTGGTACACACATCATCTCTTTGTGATCTTCTTCATTGGCCTTGCC ATCCATGGAGCTGAACGAATTGTACGTGGGCAGACCGCAGAGAGTTTGGCTGTGCATAAT ATAACAGTTTGTGAACAAAAAATCTCAGAATGGGGAAAAATAAAGGAATGCCCAATCCCT CAGTTTGCTGGAAACCCTCCTATGACTTGGAAATGGATAGTGGGTCCCATGTTTCTGTAT CTCTGTGAGAGGTTGGTGCGGTTTTGGCGATCTCAACAGAAGGTGGTCATCACCAAGGTG GTCACTCACCCTTTCAAAACCATCGAGCTACAGATGAAGAAGAAGGGGTTCAAAATGGAA GTGGGACAATACATTTTTGTCAAGTGCCCAAAGGTGTCCAAGCTGGAGTGGCACCCTTTT ACACTGACATCCGCCCCTGAGGAAGACTTCTTTAGTATCCATATCCGCATCGTTGGGGAC TGGACAGAGGGGCTGTTCAATGCTTGTGGCTGTGATAAGCAGGAGTTTCAAGATGCGTGG AAACTACCTAAGATAGCGGTTGATGGGCCCTTTGGCACTGCCAGTGAAGATGTGTTCAGC TATGAGGTGGTGATGTTAGTGGGAGCAGGGATTGGGGTCACACCCTTCGCATCCATTCTC AAGTCAGTCTGGTACAAATATTGCAATAACGCCACCAATCTGAAGCTCAAAAAGATCTAC TTCTACTGGCTGTGCCGGGACACACATGCCTTTGAGTGGTTTGCAGATCTGCTGCAACTG CTGGAGAGCCAGATGCAGGAAAGGAACAATGCCGGCTTCCTCAGCTACAACATCTACCTC ACTGGCTGGGATGAGTCTCAGGCCAATCACTTTGCTGTGCACCATGATGAGGAGAAAGAT GTGATCACAGGCCTGAAACAAAAGACTTTGTATGGACGGCCCAACTGGGATAATGAATTC AAGACAATTGCAAGTCAACACCCTAATACCAGAATAGGAGTTTTCCTCTGTGGACCTGAA GCCTTGGCTGAAACCCTGAGTAAACAAAGCATCTCCAACTCTGAGTCTGGCCCTCGGGGA GTGCATTTCATTTTCAACAAGGAAAACTTCTAA Clone variation with respect to NM_000397.3 >OriGene 5' read for NM_000397 unedited
NNNCCGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGTCAACC TCTGCCACCATGGGGAACTGGGCTGTGAATGAGGGGCTCTCCATTTTTGTCATTCTGGTT TGGCTGGGGTTGAACGTCTTCCTCTTTGTCTGGTATTACCGGGTTTATGATATTCCACCT AAGTTCTTTTACACAAGAAAACTTCTTGGGTCAGCACTGGCACTGGCCAGGGCCCCTGCA GCCTGCCTGAATTTCAACTGCATGCTGATTCTCTTGCCAGTCTGTCGAAATCTGCTGTCC TTCCTCAGGGGTTCCAGTGCGTGCTGCTCAACAAGAGTTCGAAGACAACTGGACAGGAAT CTCACCTTTCATAAAATGGTGGCATGGATGATTGCACTTCACTCTGCGATTCACACCATT GCACATCTATTTAATGTGGAATGGTGTGTGAATGCCCGAGTCAATAATTCTGATCCTTAT TCAGTAGCACTCTCTGAACTTGGAGACAGGCAAAATGAAAGTTATCTCAATTTTGCTCGA AAGAGAATAAAGAACCCTGAAGGAGGCCTGTACCTGGCTGTGACCCTGTTGGCAGGCATC ACTGGAGTTGTCATCACGCTGTGCCTCATATTAATTATCACTTCCTCCACCAAAACCATC CGGAGGTCTTACTTTGAAGTCTTTTGGTACACACATCATCTCTTTGTGATCTTCTTCATT GGCCTTGCCATCCATGGAGCTGAACGAATTGTACGTGGGCAGACCGCAGAGAGTTTTGGC TGTGCATAATATAACAGTTTGTGAACAAAAATCTCAGATGGGGAANAATAAGGATGCCCA TCCCTCAGTTGCTGGAACCCTCTATACTNGAATGATATAGTGGTCCATGGTTTGT >OriGene 3' read for NM_000397 unedited
CTTAAATTAGCCTGCTTTCTTTTATAGCACAGGAAGCAGGACTAGCATGAGCCATAGTCA TGGCAGGAGGTTAATTCTCCACCTCCAACCCTCTTTTTTCATGCTTCAAATAAGAATGTG CAGAACCCTTCAGGCAATCCTATTGGTTAGTATCCTTAGCCTGTTCTTGACCCTAGGGAG AATCAGATTCCTTTGGTCTATGACAGTAATAGAAGTTATTTCTTTAGATAAATTTTGTTT AAGGCTTTAAATTAATTCTATCTTGGCATTGTGTGGTTCTAAATTCATTCATTCATTTAA CAAAACTTACTTGAGTATCCGCTCTCTGCCAAGCACAGTGGTAAATGATGAGTATTACAT TAACTCCAATCAATCCAATATTCTTCTGTAGAAAGAACATCTGGCCCAGTTACCCTGCTG TATTAGTAAACTGGAGTATGCTCAGATGTCAATACTGTCACTAATAAGTGTTGAGACACT CTCAACATGTATTATGTCCTAATGATCTGGAAAATTTCCTTCAGTATGATAATAATGATG GCAAAAATGACACANAGTAGAAATATATAGCTACTCCTTGGTTTCCTTCTTCTTTTGGGG AATATAAAAACTGATTTTGAGGATTCTGAGGCAGCTCAGTATTGTGGAAAGAGTATCAAA CCTGGAGTAAGATGGGCTAGATCTGGTCACAGTTTCATAATCTTANGGAAGTTACTGAAA CCTTTGATCCTTTCCCTTTTGATTCTAAAATTGAGTCCAAGAAATCTTTTCCCTACTTCA CTGACATTACTGAGAGGAAAAAATGAAATACTGCTTGAAAAGTGCTCTCAAAACATAGAG CTCCCATAAATGTAACTTATCACTATCAAACAATCTTTGACATTCCNTTNAGGGGAAACC CATACATNATAGTTTCTTTTTGGTCCTTTTTAAAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000397 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000397.2, NP_000388.2 |
| RefSeq Size | 4266 bp |
| RefSeq ORF | 1713 bp |
| Locus ID | 1536 |
| UniProt ID | P04839 |
| Domains | Ferric_reduct |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Protein Pathways | Leukocyte transendothelial migration |
| Gene Summary | Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207544 | CYBB (Myc-DDK-tagged)-Human cytochrome b-245, beta polypeptide (CYBB) |
CNY 4,248.00 |
|
| RC207544L1 | Lenti ORF clone of Human cytochrome b-245, beta polypeptide (CYBB), Myc-DDK-tagged |
CNY 6,648.00 |
|
| RC207544L2 | Lenti ORF clone of Human cytochrome b-245, beta polypeptide (CYBB), mGFP tagged |
CNY 5,890.00 |
|
| RC207544L3 | Lenti ORF clone of Human cytochrome b-245, beta polypeptide (CYBB), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207544L4 | Lenti ORF clone of Human cytochrome b-245, beta polypeptide (CYBB), mGFP tagged |
CNY 6,648.00 |
|
| RG207544 | CYBB (tGFP-tagged) - Human cytochrome b-245, beta polypeptide (CYBB) |
CNY 5,848.00 |
