PPM1L (NM_139245) Human Untagged Clone

CAT#: SC125344

PPM1L (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L)



  "NM_139245" in other vectors (7)

CNY 5,800.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-PPM1L antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PP2C-epsilon; PP2CE; PPM1-LIKE
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_139245, the custom clone sequence may differ by one or more nucleotides


ATGATAGAGGATACAATGACTTTGCTGTCTCTGCTGGGTCGCATCATGCGCTACTTCTTGCTGAGACCCG
AGACGCTTTTCCTGCTGTGCATCAGCTTGGCTCTATGGAGTTACTTCTTCCACACCGACGAGGTGAAGAC
CATCGTGAAGTCCAGCCGGGACGCCGTGAAGATGGTGAAGGGCAAGGTAGCCGAGATCATGCAGAACGAT
CGACTCGGGGGGCTTGATGTGCTCGAGGCCGAGTTTTCCAAGACCTGGGAGTTCAAGAACCACAACGTGG
CGGTGTACTCCATCCAGGGCCGGAGAGACCACATGGAGGACCGCTTCGAAGTTCTCACGGATCTGGCCAA
CAAGACGCACCCGTCCATCTTCGGGATCTTCGACGGGCACGGGGGAGAGACTGCAGCTGAATATGTAAAA
TCTCGACTCCCAGAGGCCCTTAAACAGCATCTTCAGGACTACGAGAAAGACAAAGAAAATAGTGTATTAT
CTTACCAGACCATCCTTGAACAGCAGATTTTGTCAATTGACCGAGAAATGCTAGAAAAATTGACTGTATC
CTATGATGAAGCAGGCACAACGTGTTTGATTGCTCTGCTATCAGATAAAGACCTCACTGTGGCCAACGTG
GGTGACTCGCGCGGGGTCCTGTGTGACAAAGATGGGAACGCTATTCCTTTGTCTCATGATCACAAGCCTT
ACCAGTTGAAGGAAAGAAAGAGGATAAAGAGAGCAGGTGGTTTCATCAGTTTCAATGGCTCCTGGAGGGT
CCAGGGAATCCTGGCCATGTCTCGGTCCCTGGGGGATTATCCGCTGAAAAATCTCAACGTGGTCATCCCA
GACCCAGACATCCTGACCTTTGACCTGGACAAGCTTCAGCCTGAGTTCATGATCTTGGCATCAGATGGTC
TCTGGGATGCTTTCAGCAATGAAGAAGCAGTTCGATTCATCAAGGAGCGCTTGGATGAACCTCACTTTGG
GGCCAAGAGCATAGTTTTACAGTCATTTTACAGAGGCTGCCCTGACAATATAACAGTCATGGTGGTGAAG
TTCAGAAATAGCAGCAAAACAGAAGAGCAGTGA


>OriGene 3' read for NM_139245 unedited
AGGTTCACTGCTCTTCTGTTTTGCTGCTATTTCTGAACTTCACCACCATGACTGTTATAT
TGTCAGGGCAGCCTCTGTAAAATGACTGTAAAACTATGCTCTTGGCCCCAAAGTGAGGTT
CATCCAAGCGCTCCTTGATGAATCGAACTGCTTCTTCATTGCTGAAAGCATCCCAGAGAC
CATCTGATGCCAAGATCATGAACTCAGGCTGAAGCTTGTCCAGGTCAAAGGTCAGGATGT
CTGGGTCTGGGATGACCACGTTGAGATTTTTCAGCGGATAATCCCCCAGGGACCGAGACA
TGGCCAGGATTCCCTGGACCCTCCAGGAGCCATTGAAACTGATGAAACCACCTGCTCTCT
TTATCCTCTTTCTTTCCTTCAACTGGTAAGGCTTGTGATCATGAGACAAAGGAATAGCGT
TCCCATCTTTGTCACACAGGACCCCGCGCGAGTCACCCACGTTGGCCACAGTGAGGTCTT
TATCTGATAGCAGAGCAATCAAACACGTTGTGCCTGCTTCATCATANGATACAAGTCAAT
TTTCTAGCCTTTCTTGGTCCATTTGACAAAATCTGGCTGTCAAAAGATGGTTCCGGTAAA
GAAAATTCCACCAATTTTCTTTGGCCTTTCCGCGAAATCCCGGAGGAAGCCGGTTAAANG
GGCCTTCTGGGAAGTCAAAGATTTAACCATATTTCAAGTGCAGTTCTTTTCCCCCCGTGC
CCGTTCAAAAATTCCCCAAATATGGCACCGGTCCGCTCTTGGTGGGCCACATTCCCGGGA
GAACT
Restriction Sites Please inquire     
ACCN NM_139245
Insert Size 2700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_139245.1, NP_640338.1
RefSeq Size 3056 bp
RefSeq ORF 546 bp
Locus ID 151742
UniProt ID Q5SGD2
Domains PP2C
Protein Families Druggable Genome, Phosphatase
Gene Summary The protein encoded by this gene is a magnesium or manganese-requiring phosphatase that is involved in several signaling pathways. The encoded protein downregulates apoptosis signal-regulating kinase 1, a protein that initiates a signaling cascade that leads to apoptosis when cells are subjected to cytotoxic stresses. This protein also is an endoplasmic reticulum transmembrane protein that helps regulate ceramide transport from the endoplasmic reticulum to the Golgi apparatus. Finally, this gene may be involved in adiposity since it is upregulated in adipose tissues. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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