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PPM1L (NM_139245) Human Untagged Clone
CAT＃: SC317574
PPM1L (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L)
CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PP2C-epsilon; PP2CE; PPM1-LIKE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317574 representing NM_139245.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATAGAGGATACAATGACTTTGCTGTCTCTGCTGGGTCGCATCATGCGCTACTTCTTGCTGAGACCC GAGACGCTTTTCCTGCTGTGCATCAGCTTGGCTCTATGGAGTTACTTCTTCCACACCGACGAGGTGAAG ACCATCGTGAAGTCCAGCCGGGACGCCGTGAAGATGGTGAAGGGCAAGGTAGCCGAGATCATGCAGAAC GATCGACTCGGGGGGCTTGATGTGCTCGAGGCCGAGTTTTCCAAGACCTGGGAGTTCAAGAACCACAAC GTGGCGGTGTACTCCATCCAGGGCCGGAGAGACCACATGGAGGACCGCTTCGAAGTTCTCACGGATCTG GCCAACAAGACGCACCCGTCCATCTTCGGGATCTTCGACGGGCACGGGGGAGAGACTGCAGCTGAATAT GTAAAATCTCGACTCCCAGAGGCCCTTAAACAGCATCTTCAGGACTACGAGAAAGACAAAGAAAATAGT GTATTATCTTACCAGACCATCCTTGAACAGCAGATTTTGTCAATTGACCGAGAAATGCTAGAAAAATTG ACTGTATCCTATGATGAAGCAGGCACAACGTGTTTGATTGCTCTGCTATCAGATAAAGACCTCACTGTG GCCAACGTGGGTGACTCGCGCGGGGTCCTGTGTGACAAAGATGGGAACGCTATTCCTTTGTCTCATGAT CACAAGCCTTACCAGTTGAAGGAAAGAAAGAGGATAAAGAGAGCAGGTGGTTTCATCAGTTTCAATGGC TCCTGGAGGGTCCAGGGAATCCTGGCCATGTCTCGGTCCCTGGGGGATTATCCGCTGAAAAATCTCAAC GTGGTCATCCCAGACCCAGACATCCTGACCTTTGACCTGGACAAGCTTCAGCCTGAGTTCATGATCTTG GCATCAGATGGTCTCTGGGATGCTTTCAGCAATGAAGAAGCAGTTCGATTCATCAAGGAGCGCTTGGAT GAACCTCACTTTGGGGCCAAGAGCATAGTTTTACAGTCATTTTACAGAGGCTGCCCTGACAATATAACA GTCATGGTGGTGAAGTTCAGAAATAGCAGCAAAACAGAAGAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_139245 |
| Insert Size | 1083 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139245.3 |
| RefSeq Size | 10945 bp |
| RefSeq ORF | 1083 bp |
| Locus ID | 151742 |
| UniProt ID | Q5SGD2 |
| Domains | PP2C |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 41.1 kDa |
| Gene Summary | The protein encoded by this gene is a magnesium or manganese-requiring phosphatase that is involved in several signaling pathways. The encoded protein downregulates apoptosis signal-regulating kinase 1, a protein that initiates a signaling cascade that leads to apoptosis when cells are subjected to cytotoxic stresses. This protein also is an endoplasmic reticulum transmembrane protein that helps regulate ceramide transport from the endoplasmic reticulum to the Golgi apparatus. Finally, this gene may be involved in adiposity since it is upregulated in adipose tissues. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218909 | PPM1L (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L) |
CNY 3,656.00 |
|
| RC218909L1 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC218909L2 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L), mGFP tagged |
CNY 5,890.00 |
|
| RC218909L3 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218909L4 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L), mGFP tagged |
CNY 5,890.00 |
|
| RG218909 | PPM1L (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L) |
CNY 5,256.00 |
|
| SC125344 | PPM1L (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1L (PPM1L) |
CNY 5,800.00 |
