ZNF195 (NM_007152) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HRF1; ZNFP104
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC126999 sequence for NM_007152 edited (data generated by NextGen Sequencing) ATGACTCTGTTGACGTTCAGGGATGTGGCCATAGAATTCTCCCTGGAGGAGTGGAAATGC CTGGACCTCGCTCAGCAGAATTTGTACAGGGATGTGATGTTGGAGAACTACAGAAACTTG TTCTCCGTTGGTCTCACTGTCTGTAAGCCAGGCCTGATCACCTGCCTGGAGCAACGAAAA GAGCCCTGGAATGTGAAGAGACAGGAGGCAGCAGACGGACATCCAGCTATGTCTTCTCAT TTTACCCAAGACCTTCTGCCAGAGCAGGGCATACAAGATGCATTCCCAAAAAGAATACTG AGAGGATATGGAAATTGTGGCCTTGATAATTTATATTTAAGGAAAGACTGGGAAAGTTTA GATGAGTGTAAGTTGCAAAAAGATTATAATGGACTTAACCAATGTTCATCAACTACCCAT AGCAAAATCTTTCAATATAATAAATATGTTAAAATCTTTGATAACTTTTCAAATTTACAT AGACGTAATATAAGTAATACTGGAGAGAAACCTTTCAAATGTCAAGAATGTGGCAAATCC TTTCAAATGCTCTCATTCCTAACTGAACATCAGAAAATTCACACTGGAAAAAAATTCCAA AAATGTGGAGAATGTGGCAAAACCTTTATCCAGTGCTCACACTTTACTGAACCTGAGAAC ATTGACACTGGAGAGAAACCTTACAAGTGTCAAGAATGTAACAACGTCATTAAAACTTGC TCAGTCCTTACTAAAAATAGAATTTATGCCGGAGGGGAACATTACAGATGTGAAGAATTT GGCAAAGTATTTAACCAGTGCTCCCACCTTACTGAACATGAGCATGGTACTGAGGAAAAA CCCTGCAAATATGAAGAGTGCAGCAGTGTCTTTATCTCTTGCTCAAGCCTTTCTAATCAA CAGATGATTCTTGCTGGAGAGAAGCTCTCCAAATGTGAAACATGGTACAAAGGTTTTAAC CACAGCCCAAATCCTTCCAAACACCAGAGAAATGAGATTGGAGGGAAACCTTTCAAATGT GAGGAATGTGACAGCATCTTCAAGTGGTTCTCAGACCTTACTAAACATAAGAGAATTCAC ACTGGTGAGAAACCATACAAATGTGACGAATGTGGGAAAGCCTATACACAGTCCTCACAC CTCAGTGAACACAGGAGGATTCACACCGGAGAGAAACCCTACCAATGTGAAGAATGTGGG AAGGTCTTCAGAACTTGCTCAAGCCTTTCTAACCATAAGAGAACTCATTCTGAAGAAAAA CCCTACACGTGTGAAGAATGTGGCAACATCTTTAAGCAGTTATCAGACCTCACTAAGCAT AAGAAAACCCATACTGGAGAGAAGCCCTACAAATGTGACGAATGTGGAAAAAACTTTACC CAGTCCTCCAACCTTATTGTACATAAGAGAATTCATACTGGAGAGAAACCCTACAAGTGT GAAGAATGTGGCAGAGTCTTCATGTGGTTCTCAGACATTACCAAACATAAGAAAACCCAT ACTGGAGAGAAACCCTACAAATGTGACGAATGTGGAAAAAACTTTACCCAGTCCTCAAAC CTTATTGTACATAAGAGAATTCATACTGGAGAGAAACCCTACAAGTGTGAAAAGTGTGGC AAAGCCTTCACCCAGTTCTCACACCTGACTGTACATGAAAGCATTCATACTTGA Clone variation with respect to NM_007152.4 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_007152 unedited ATTTTGTAATACGAACTCCTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCCCTGCTAG CCGTAGGCCGTGTGACCCGCAGGCACCGGGAGATCCAGAAGTGAAACGCCAGGCTCTCTG GAGGCCAGGAGATGACTCTGTTGACGTTCAGGGATGTGGCCATAGAATTCTCCCTGGAGG AGTGGAAATGCCTGGACCTCGCTCAGCAGAATTTGTACAGGGATGTGATGTTGGAGAACT ACAGAAACTTGTTCTCCGTTGGTCTCACTGTCTGTAAGCCAGGCCTGATCACCTGCCTGG AGCAACGAAAAGAGCCCTGGAATGTGAAGAGACAGGAGGCAGCAGACGGACATCCAGCTA TGTCTTCTCATTTTACCCAAGACCTTCTGCCAGAGCAGGGCATACAAGATGCATTCCCAA AAAGAATACTGAGAGGATATGGAAATTGTGGCCTTGATAATTTATATTTAAGGAAAGACT GGGAAAGTTTAGATGAGTGTAAGTTGCAAAAAGATTATAATGGACTTAACCAATGTTCAT CAACTACCCATAGCAAAATCTTTCAATATAATAAATATGTTAAAATCTTTGATAACTTTT CAAATTTACATAGACCGTATATAAGTAATACTGGAGAGAAACCTTTCAAATGTCAAGAAT GTGGCANATCCTTTCAAATGCTCTCATTCCTAACTGAACATCAGAAAATTCACACTGGAA AAAAATTCCAAAAATGTGGAGAATGTGGCANAACCTTTATCCAGTGCTCACACTTTACCT GACCTGAGAACATTGACACTGGNAGAGAACCTTACCAGTGTCAAGAATGGTACAACGTCA TTAAAACTTGCTCAGTCCTTACTAAAATAGAANTTATGCCCGAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_007152 unedited GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTCTCTACCAAAATTT TACTCACGCATCTTACATTTCAGTGTCTTTCACCTTTCACGGACAAGTATATCAGTGATG CACACCTAAGAGAAAAGAATTTCTCATATCTCTGACGCAGCAACAACTGACCATGTGTTT TCACATCAGCACTAGAAATGAAGACACAGCATCTACTGATTTGAGAATAGAATGACATCA ATATTCGCTTTTCAAAAATTTAACATTTTTTCAATGCAAAAAAGTATATTTTGAATGTAA TTATAACTCTACAAAAACTCAGTCTCATCCTCCTTTTAAACTTAAATATAAATGATCTAT TTTAAGTCTAGATGTCTCTGTAGACTCAACACAGGGATTCAGTGTCATTTCTGAACGTGT CCGTGCTCTATTCCTTTCTCCTTCAAATCCTCCATTGTTTACTTAAATTGGATTTTGCTT ATTTTTCCATTTAGTGTATTTGCAAAATATCTTTTAGTATGAACTCTAGTGTTTTCTAAG CTATCATTTTTGAACAAATGTTATTCCACATTTATGAGATTTGTTGGGTTTCTCTACAAA TTTTCTGAAAGTTTAAGCAACTGATGCAAGTCTTCCATCTAGTATAATTGTAACATTTTT TTCAGAAGAAATACTCTTGTGTGCTCTGGAGACTTATATTTCATGAAAGGTCTTTCAATA GTAATTACATTTATGATAACTTTATTAAGTCTGAACTCTGATGTAAAGTGGGGATGCGAG CAGATACTAACGGCCTTGCCCTATTTTATATTGGTTATTTCTTTCTCAGTATGAATGCTT TCATTACAGTCAGGTGTGAGACTGGGTGAGGCTTTGCCCACTTTTACACTTGTAGGTTCT CTCCATATGAATCTCTTAGACATAAGGTTGG
Restriction Sites	NotI-NotI
ACCN	NM_007152
Insert Size	2560 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007152.1, NP_009083.1
RefSeq Size	2394 bp
RefSeq ORF	1890 bp
Locus ID	7748
UniProt ID	O14628
Domains	KRAB, zf-C2H2
Protein Families	Transcription Factors
Gene Summary	This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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