UNC13B (NM_006377) Human Untagged Clone

CAT#: SC127356

UNC13B (untagged)-Human unc-13 homolog B (C. elegans) (UNC13B)



  "NM_006377" in other vectors (4)

CNY 10,896.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
Rabbit Polyclonal antibody to UNC13B (unc-13 homolog B (C. elegans))
    • 100 ul

CNY 6,281.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MUNC13; munc13-2; UNC13; Unc13h2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC127356 sequence for NM_006377 edited (data generated by NextGen Sequencing)
ATGTCACTGCTCTGCGTGCGCGTTAAAAGGGCCAAATTCCAGGGTTCACCAGATAAATTT
AACACATATGTGACCCTGAAAGTACAGAATGTGAAGAGCACAACTGTAGCAGTTCGTGGT
GATCAGCCTTCCTGGGAACAGGATTTCATGTTTGAGATTAGTCGCCTGGACCTGGGTCTA
AGTGTGGAGGTATGGAACAAAGGACTGATCTGGGACACCATGGTGGGGACTGTGTGGATT
GCGCTGAAGACTATTCGTCAGTCGGATGAGGAAGGGCCTGGGGAATGGTCCACATTAGAG
GCAGAGACGTTAATGAAAGACGATGAGATCTGTGGAACTAGAAACCCAACTCCTCATAAA
ATTTTGCTTGATACAAGATTTGAGTTGCCTTTTGATATCCCAGAGGAGGAAGCCAGATAT
TGGACCTACAAATGGGAGCAAATCAATGCCTTGGGAGCTGACAATGAGTATTCTAGTCAA
GAAGAAAGCCAGAGGAAGCCATTGCCCACTGCTGCCGCCCAGTGTTCTTTTGAAGACCCT
GATAGTGCCGTCGATGACCGAGATAGTGACTATCGCAGTGAGACCAGCAACAGCTTCCCA
CCTCCTTACCATACAGCTTCCCAGCCCAACGCTTCTGTGCACCAGTTCCCTGTGCCGGTG
CGATCGCCACAGCAGCTGCTACTTCAAGGCAGTTCCCGGGACTCTTGTAATGACTCTATG
CAAAGTTATGACCTTGATTATCCAGAGCGGCGGGCTATCAGCCCCACCAGCAGCAGTAGG
TATGGCTCCTCCTGTAATGTGAGTCAAGGAAGCTCTCAGCTAAGTGAACTAGACCAGTAT
CACGAACAAGATGACGACCATCGGGAGACGGACTCGATTCATTCTTGCCACAGCTCTCAC
AGCCTGTCCAGAGATGGCCAAGCAGGTTTTGGAGAACAAGAGAAACCCTTGGAGGTGACA
GGTCAAGCAGAGAAGGAGGCAGCATGTGAACCCAAGGAGATGAAAGAAGATGCCACAACC
CACCCTCCCCCAGATCTGGTGCTGCAAAAAGACCACTTCCTAGGTCCCCAGGAGAGTTTT
CCTGAGGAGAATGCATCTTCACCATTTACCCAAGCCAGAGCACATTGGATCCGAGCAGTT
ACCAAGGTTCGACTCCAGCTGCAGGAGATTCCAGATGATGGTGACCCCTCTCTGCCTCAG
TGGCTCCCGGAAGGGCCAGCCGGAGGGCTCTATGGCATTGACAGCATGCCAGATTTACGC
AGAAAGAAGCCACTGCCACTTGTCAGTGATCTGTCACTGGTCCAGTCTCGGAAGGCAGGA
ATCACTTCTGCAATGGCTACACGCACTTCTCTTAAGGACGAAGAGCTGAAATCCCACGTG
TATAAGAAAACCCTGCAGGCCTTAATCTACCCCATTTCGTGCACCACTCCTCATAACTTT
GAGGTCTGGACGGCCACTACCCCAACCTACTGCTATGAGTGTGAAGGCCTGCTCTGGGGC
ATTGCCCGGCAGGGCATGCGCTGCAGCGAATGTGGAGTCAAGTGCCATGAGAAGTGCCAG
GATCTGCTCAATGCTGACTGCCTGCAGCGGGCTGCAGAAAAGAGCTGTAAACATGGAGCT
GAGGACCGGACCCAGAACATTATCATGGCCATGAAGGACCGCATGAAGATCCGAGAGCGA
AATAAGCCAGAGATCTTTGAAGTTATCCGGGACGTCTTCACAGTGAACAAAGCTGCCCAT
GTGCAGCAGATGAAAACAGTGAAGCAGAGTGTACTGGATGGCACCTCCAAATGGTCAGCC
AAGATCACCATTACTGTGGTGTGTGCCCAGGGCCTACAAGCCAAGGACAAAACAGGATCC
AGTGACCCTTACGTGACTGTGCAAGTCAGCAAAACTAAGAAGCGTACCAAGACCATTTTT
GGAAACTTGAATCCTGTTTGGGAGGAGAAGTTCCATTTTGAGTGCCACAACTCCTCTGAC
CGCATTAAGGTGCGTGTATGGGATGAGGATGATGACATCAAGTCAAGAGTAAAGCAACGC
CTAAAGCGAGAGTCTGATGATTTCCTTGGCCAAACCATCATTGAGGTTCGGACCCTAAGT
GGCGAGATGGACGTCTGGTACAACTTGGAGAAGAGGACAGACAAATCAGCCGTCTCAGGG
GCTATCCGACTACAAATCAGTGTGGAGATCAAGGGGGAGGAGAAAGTAGCCCCATACCAC
GTGCAGTATACATGTCTCCATGAGAATCTTTTCCATTACCTCACAGACATTCAGGGCAGT
GGAGGAGTCCGCATCCCTGAAGCTCGAGGAGACGATGCCTGGAAGGTGTACTTTGATGAG
ACAGCCCAAGAAATTGTGGATGAATTTGCCATGCGTTATGGCATTGAGTCCATATATCAG
GCCATGACGCACTTTGCATGTTTATCATCCAAGTACATGTGTCCTGGTGTGCCAGCAGTG
ATGAGCACCTTACTGGCCAACATCAACGCCTACTATGCCCACACAACTGCCTCTACCAAT
GTCTCTGCATCTGATCGCTTTGCAGCCTCCAACTTTGGGAAAGAGAGATTTGTAAAACTG
CTGGACCAGCTACACAACTCACTGAGGATCGACCTCTCTACATACAGGAATAATTTCCCT
GCTGGGAGTCCTGAACGGCTTCAGGACTTAAAATCCACAGTGGATTTGCTGACCAGCATT
ACTTTCTTCAGAATGAAGGTACAAGAACTGCAAAGCCCTCCAAGAGCCAGCCAGGTGGTA
AAGGATTGTGTGAAGGCCTGTTTGAACTCCACATATGAATATATCTTCAACAACTGCCAC
GACTTATACAGCCGCCAGTACCAGCTGAAGCAGGAGCTACCTCCAGAGGAACAAGGGCCC
AGCATTCGGAACCTGGATTTCTGGCCCAAGCTCATCACACTCATCGTGTCAATCATAGAG
GAAGATAAGAATTCCTACACACCTGTTCTGAACCAGTTTCCTCAGGAGTTGAATGTGGGA
AAAGTCAGCGCAGAAGTGATGTGGCATTTGTTTGCCCAAGACATGAAATATGCATTGGAG
GAGCATGAGAAAGACCACCTGTGTAAAAGTGCTGACTACATGAACCTGCACTTCAAGGTG
AAGTGGCTCCACAATGAATACGTGCGGGATCTGCCTGTCCTCCAGGGGCAGGTGCCTGAG
TACCCAGCGTGGTTTGAGCAGTTCGTGCTACAATGGCTGGATGAGAATGAGGATGTATCC
CTGGAATTCCTGCGTGGGGCCCTGGAACGAGATAAGAAGGATGGATTCCAGCAGACATCA
GAGCATGCACTCTTTTCCTGCTCTGTGGTGGATGTCTTCACACAACTCAATCAGAGCTTT
GAGATCATCCGGAAGCTGGAATGCCCAGACCCCAGCATCCTTGCCCACTACATGAGGAGG
TTTGCTAAGACCATCGGGAAGGTGCTGATGCAGTATGCAGACATCTTGTCAAAGGACTTC
CCAGCCTATTGCACAAAGGAGAAACTGCCCTGCATCCTGATGAACAACGTGCAGCAACTG
AGGGTCCAGCTGGAGAAAATGTTTGAGGCCATGGGAGGCAAGGAGCTGGACCTTGAAGCT
GCAGACAGTCTGAAGGAGCTGCAGGTGAAACTGAATACGGTTCTGGATGAGCTCAGCATG
GTGTTTGGAAACAGTTTCCAGGTACGGATTGATGAGTGTGTTCGACAAATGGCCGACATC
CTGGGCCAGGTTCGGGGCACAGGGAATGCATCTCCAGACGCCAGGGCCTCAGCGGCTCAG
GATGCAGATAGCGTACTCCGGCCTCTCATGGACTTCCTGGATGGCAACCTCACCCTCTTT
GCCACTGTGTGTGAGAAGACGGTTCTGAAGCGTGTACTGAAGGAGCTCTGGCGCGTGGTG
ATGAACACAATGGAGAGGATGATTGTTCTGCCCCCACTCACTGACCAGACGGGCACCCAG
CTGATCTTCACTGCTGCCAAGGAGCTGAGCCATCTTTCCAAACTCAAGGATCACATGGTA
CGAGAGGAAACACGGAATCTCACTCCAAAGCAGTGTGCAGTCCTTGACCTCGCCCTGGAC
ACCATCAAGCAATACTTCCATGCAGGAGGCAATGGGCTGAAGAAAACCTTCCTGGAGAAG
AGCCCAGATCTGCAGTCTCTACGCTATGCCCTGTCTCTGTACACACAGACTACTGACACT
CTCATCAAGACCTTTGTGCGCTCGCAGACCACCCAAGGGTCTGGTGTGGACGATCCTGTG
GGAGAAGTCTCTATTCAGGTGGACTTGTTTACACACCCTGGTACTGGGGAGCACAAGGTC
ACAGTGAAAGTGGTGGCTGCCAATGACCTCAAGTGGCAGACAGCGGGTATGTTCCGGCCT
TTCGTGGAGGTGACTATGGTTGGCCCACACCAAAGTGATAAGAAGAGGAAGTTCACAACC
AAATCCAAAAGCAACAACTGGGCCCCCAAGTACAATGAGACATTCCACTTCCTCCTGGGA
AATGAGGAGGGGCCCGAGTCCTATGAGTTGCAGATATGCGTGAAGGATTACTGCTTTGCC
CGGGAAGATCGCGTGCTAGGGCTGGCTGTGATGCCTCTGAGGGATGTCACAGCCAAGGGC
AGCTGTGCCTGCTGGTGCCCCTTGGGCCGGAAGATCCATATGGATGAGACAGGCCTGACC
ATTCTCCGGATTTTATCTCAGAGGAGCAATGACGAGGTGGCCCGAGAATTTGTGAAACTC
AAATCAGAGTCTCGTTCCACGGAGGAGGGGAGCTGA

Clone variation with respect to NM_006377.3
>OriGene 5' read for NM_006377 unedited
CGGCCGCGAATTCGGCACGAGCTTGCCCGATCCTCGGCCATGTCACTGCTCTGCGTGCGC
GTTAAAAGGGCCAAATTCCAGGGTTCACCAGATAAATTTAACACATATGTGACCCTGAAA
GTACAGAATGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAACAG
GATTTCATGTTTGAGATTAGTCGCCTGGACCGGGGTCTAAGTGTGGAGGTATGGAACAAA
GGACTGATCTGGGACACCATGGTGGGGACTGTGTGGATTGCGCTGAAGACTATTCGTCAG
TCGGATGAGGAAGGGCCTGGGGAATGGTCCACATTAGAGGCAGAGACGTTAATGAAAGAC
GATGAGATCTGTGGAACTAGAAACCCAACTCCTCATAAAATTTTGCTTGATACAAGATTT
GAGTTGCCTTTTGATATCCCAGAGGAGGAAGCCAGATATTGGACCTACAAATGGGAGCAA
ATCAATGCCTTGGGAGCTGACAATGAGTATTCTAGTCAAGAAGAAAGCCAGAGGAAGCCA
TTGCCCACTGCTGCCGCCCAGTGTTGTCACTGGACCTATTTGGGCTGGGGAGAACAACAG
ACTTTTGAAGACCCTGATAGTGCCGTCGATGACCGAGATAGTGACTATCGCAGTGAGACC
CAGCACAGCTTCCCACCTCCCTACCATACAGCTTCCCAGCCCAACGCTTNTGTGCACN
>OriGene 3' read for NM_006377 unedited
TAGCTATGNAACGCGGCACGCAATCTAGTGATCGGTTTTTTTTTTTTTTTTTTTGGGTTC
TTGTTTCATGTAATGGCCAGTACATCTGTGGGCAATGTCGAGTCCTCAGGAAGTCCAGGA
GGCTGCTACAGAGGAAATCCAAGAACCATGTCACATCTCTCAACAAGTCTTGGGAAGTCC
ATCTGACTCTCTGAAACAGTTTGTCTCTGACCTCCCAGGAAGTGTGGAGGGCCCCTTCCA
TCCAGCCTGTACAGAGGGATCAGAGTCCAGGCTCCTTCTATAGGGTTGAATATCAGAGGG
GAATAGCAAATGACCCCGATGAGAGAGAGAGAGACCAAAGGCTAGATTCTTTCTGCAAGG
TGGAGGACGGCTAGAAGGCAGTGGCCACAGATGAGCAGGACTTTTTCTAGACTGGTTTCT
ACAATTTTTGGCTTTGTTCAGGGCCCACCCAAGAAGAAAATACTCCTGGATCCTTCAACT
AGCCCTCAGGTAGACACATGTTAGAACAGCCTGTCACGTCCTGAAGCATCACTCCCCTGC
CTATAGGAGTGAGGCAGGGAGACTCCCATGCAGGTGGCCCCTAGTGCCTGGGGTCCAAAG
AAATATTCTTCTCAAGGCTTTGAAGCACCACGTGAACCACATTCTACTCATGATACAATC
TGGGGAAAGGGATTAGTACAGCCCATTTCCTAAAGGTATCCTGTTCAGTTTCAAAGCCCC
TGCTCTCACTGGAGCCACAGAAAATCAGTGGATTCTACCCAAAGGACAGGCTAAATTCTT
TTCTGTGGACTCCTGCCCTGGCCCTACTCCCATTCAGACCACCGGCTTAACACCAGGGCT
AGCTGGCTTACCCAGCCGCCAGGCCCCAAGCCCCCACCTCTCAAACTTGGTCCTTCAATG
GTGAGGTTGTATGCGTCCACATGAAAACGCGTTGCCAGCATATGGTCAAAGGGGGTACTA
TTATTTCTCACCCTCCCTCTACA
Restriction Sites NotI-NotI     
ACCN NM_006377
Insert Size 6360 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006377.2, NP_006368.2
RefSeq Size 6328 bp
RefSeq ORF 4776 bp
Locus ID 10497
UniProt ID O14795
Domains C2, DAG_PE-bind
Protein Families Druggable Genome
Gene Summary This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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