Caldesmon (CALD1) (NM_033138) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_033138, the custom clone sequence may differ by one or more nucleotides GAATTCGGCACSAGCTTGCTCTCTGGCTGTGCTCCTGCTTAAAGAAATCAGTCCTTCCTTTCCGACTTAG TCCTCGGGAAGAAGTTTCAGACTACAAGGTATCATTGGAACATTTCAAGATCATCAAATCAAATTCCACA GGGATTGGTGACCAACCAGAAGGCTCAGACATCTGATTGCTGACCTGTCCAGACATCATCTGGTCTCCCT GAACCTGAAATCACACCATGGATGATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGAT GCGACTCGAAGCAGAAAGAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGG CGCCGCCGAGCCCGACAGGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGACCGACC AGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCACCACAAACACTCAAGT GGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCCTGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTT CAGGAGGCTCTGGAGCGGCAGAAGGAGTTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCA GAAGAATGCAAAATGACACAGCAGAAAATGAAACTACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGA AAGATACGAGATAGAGGAAACAGAAACAGTCACCAAGTCCTACCAGAAGAATGATTGGAGGGATGCTGAA GAAAACAAGAAAGAAGACAAGGAAAAGGAGGAGGAGGAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAG AAAATCAGGTAGAGGTGATGGTGGAAGAGAAAACAACTGAAAGCCAGGAGGAAACAGTGGTAATGTCATT AAAAAATGGGCAGATCAGTTCAGAAGAGCCTAAACAAGAGGAGGAGAGGGAACAAGGTTCAGATGAGATT TCCCATCATGAAAAGATGGAAGAGGAAGACAAGGAAAGAGCTGAGGCAGAGAGGGCAAGGTTGGAAGCAG AAGAAAGAGAAAGAATTAAAGCCGAGCAAGACAAAAAGATAGCAGATGAACGAGCAAGAATTGAAGCAGA AGAAAAAGCAGCTGCCCAAGAAAGAGAAAGGAGAGAGGCAGAAGAGAGGGAAAGGATGAGGGAGGAAGAG AAAAGGGCAGCAGAGGAGAGGCAGAGGATAAAGGAGGAAGAGAAAAGGGCAGCAGAGGAGAGGCAGAGGA TAAAGGAGGAAGAGAAAAGGGCAGCAGAGGAGAGGCAGAGGATAAAAGAGGAAGAGAAAAGGGCAGCAGA GGAGAGGCAAAGGGCCAGGGCAGAGGAGGAAGAGAAGGCTAAGGTAGAAGAGCAGAAACGTAACAAGCAG CTAGAAGAGAAAAAACGTGCCATGCAAGAGACAAAGATAAAAGGGGAAAAGGTAGAACAGAAAATAGAAG GGAAATGGGTAAATGAAAAGAAAGCACAAGAAGATAAACTTCAGACAGCTGTCCTAAAGAAACAGGGAGA AGAGAAGGGAACTAAAGTGCAAGCTAAAAGAGAAAAGCTCCAAGAAGACAAGCCTACCTTCAAAAAAGAA GAGATCAAAGATGAAAAGATTAAAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTCATGGATCGAA AGAAGGGATTTACAGAAGTTAAGTCGCAGAATGGAGAATTCATGACCCACAAACTTAAACATACTGAGAA TACTTTCAGCCGCCCTGGAGGGAGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCCCAGGTGGAA GCCGGCAAAAGGCTGGAGGAGCTTCGTCGTCGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCA AACAGAAGCAGCAGGAGGCGGCTTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGAAGGAAGGT CCTGGAGGAGGAAGAGCAGAGGAGGAAGCAGGAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAG AGGAGGCTAAAGGAAGAGATTGAAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAGATGCCAGAAG ATGGCTTGTCAGATGACAAGAAACCATTCAAGTGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGA GCGAGCAGAATTTTTGAATAAGTCTGTGCAGAAAAGCAGTGGTGTCAAATCGACCCATCAAGCAGCAATA GTCTCCAAGATTGACAGCAGACTGGAGCAGTATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTA CAAAGCCGGCAGCCTCGGATCTTCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATGTGGGAGAA AGGGAATGTGTTTTCATCCCCCACTGCAGCAGGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGG GTTTCTAGCCGCATCAATGAATGGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCTCCCAAACCTT CTGACTTGAGACCAGGAGACGTATCCAGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCAC TTCCCCCACTAAGGTTTGAGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_033138 unedited GGCCGCGAATTCGGCACCAGCTTGCTCTCTGGCTGTGCTCCTGCTTAAAGAAATCAGTCC TTCCTTTCCGACTTAGTCCTCGGGAAGAAGTTTCAGACTACAAGGTATCATTGGAACATT TCAAGATCATCAAATCAAATTCCACAGGGATTGGTGACCAACCAGAAGGCTCAGACATCT GATTGCTGACCTGTCCAGACATCATCTGGTCTCCCTGAACCTGAAATCACACCATGGATG ATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGATGCGACTCGAAGCAG AAAGAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCC GCCGAGCCCGACAGGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGA CCGACCAGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCA CCACAAACACTCAAGTGGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCCTGGCTCGGC GTGAGGAAAGACGCCAAANACGCCTTCAGGAGGCTCTGGAGCGGCAGAAGGAGTTCGACC CAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGACACAGCAG AAAATGAAACTACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGAAAGATACGAGATAG AGGAAACAGAAACAGTCACCAAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_033138 unedited CATTCGTCTGCACCGCGGCCCGCAATCTAGACCCGAGCTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTGCAGGAAGGAATTCCATTTATTGGGGATGCATTTTCACAATATATGTTTA TTGGAGCGATCCATTATCAGTGAAAAGTATCAAGTGTTTATAAAATTTTTAGGAATGGCA GATTCACAGAACATGCTAGTCAGCTTGCAGTTTTACCTTGTAAAGATAACAGAGAATTAT AGTCTAACCAGTAAACAAGGAATTTACTTTTCAAAAGATTAAATCCAAACTGAACAAAAT TTTACCCTAAAACTTACTCCATCCAAATATTGGAATAAAAGTCAGCAGTGATACATTCTT TTTTGAACTTTAGATTTTCTAGAAAAATATGTAATAGTGATCAGGAGGAGCTTTTGTTCA AAAGTACCACCAAGCAATGTTACCTTACCATAGGCCTTAATTCAAACTTTGATCCATTTC ACTCCAATGACGGGCGTCAATGCTACCTGGGACACTTGTATTTGTAAAATCTGATTTAGC TTATTGGAGACTTGTGCCTACTTTGTCATGAGGGTTTGACTTCTGCATTCTTCGNGGCTT TCCTTCCTTTGGCTTAGGTTTGTTAAAGCTAGAAGATTCCATTGCTCTTTACAGACTTAT GAGGAAAATACCCTTTGCATCGCAGAAGTCCCTTTTCAAGGCACCCCTGCCCTGGTTAGC TTTTTTTGGGAGAAACCTGCCGATTAACCACAACCATACTTCGGGCGGCTCCCTCCCGGT GTCACGAAACTGACCACCCCCACCCTTGTTAAATCCCCTTGACCATGAATCTATGCCACC CCCCTGTTGCACCCCCCTTTTATCAAATTTTTGCCCCCCCATTTGGCACCCTCTATTTTC TTCTGGGACACAATCATTCTCGGCCTTCACGACCCCCCGCACTCCTCGG
Restriction Sites	NotI-NotI
ACCN	NM_033138
Insert Size	4860 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_033138.2, NP_149129.2
RefSeq Size	5241 bp
RefSeq ORF	5241 bp
Locus ID	800
UniProt ID	Q05682
Domains	Caldesmon
Protein Pathways	Vascular smooth muscle contraction
Gene Summary	This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (1, also known as aorta h-CaD). It is predominantly expressed in smooth muscle tissues.

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