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VAMP1 (NM_199245) Human Untagged Clone
CAT＃: SC127642
VAMP1 (untagged)-Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 2
CNY 1,200.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMS25; SPAX1; SYB1; VAMP-1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127642 sequence for NM_199245 edited (data generated by NextGen Sequencing)
ATGTCTGCTCCAGCTCAGCCACCTGCTGAAGGGACAGAAGGGACTGCCCCAGGTGGGGGT CCCCCTGGCCCTCCTCCTAACATGACCAGTAACAGACGACTACAGCAAACCCAGGCACAA GTGGAGGAGGTGGTGGACATCATACGTGTGAACGTGGACAAGGTCCTGGAGAGGGACCAG AAGCTGTCAGAGCTGGATGACCGAGCTGATGCCTTGCAGGCAGGAGCATCACAATTTGAG AGCAGTGCTGCAAAGCTAAAGAGGAAGTATTGGTGGAAAAACTGCAAGATGATGATCATG CTGGGAGCCATCTGTGCCATCATCGTGGTAGTTATTGTAAGTAAGTATCGCTGA Clone variation with respect to NM_199245.1 252 c=>a >OriGene 5' read for NM_199245 unedited
NTTGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCACTGAA GGATCCTCACAGCAACCGCTCCTTTCCGGAGTCGGATGAGAGGAGAGTTGTGACTGGCAA TTGGCAGGGGCGGGGCGGGCTAGGCCTGTAGCGCTGGGCGACCGTCCTGGGCATGGATTG GGCCGCGGGGTTGTCACCGTTATCCGGGAGGCGTGGTCAGCACTAATAAAGGCGGAGGCC GGCGCGGCAGCTGCAGTAAGTTCCAGCGCAGCTAGACCGCGGGGTAGTCGGCGCGAGGCG GAGCTTGGCAGTTCCGTCCACTTCAGCCGCAGCGTCCCTCGCCGGGTGTCTCGCCGCAGC CTCCGGAGAGGAACAGACCCTCACTCTCTCTGTCAGAAAAATGTCTGCTCCAGCTCAGCC ACCTGCTGAAGGGACAGAAGGGACTGCCCCAGGTGGGGGTCCCCCTGGCCCTCCTCCTAA CATGACCAGTAACAGACGACTACAGCAAACCCAGGCACAAGTGGAGGAGGTGGTGGACAT CATACGTGTGAACGTGGACAAGGTCCTGGAGAGGGACCAGAAGCTGTCAGAGCTGGATGA CCGAGCTGATGCCTTGCAGGCAGGAGCATCACAATTTGAGAGCAGTGCTGCAAAGCTAAA GAGGAAGTATTGGTGGAAAAACTGCAAGATGATGATCATGCTGGGAGCCATCTGTGCCTC ATCGTGGTAGTTATTGTAAGTAAGTATCGCTGAAGTTGCTGGTGGNGTGAAAGTGGGGAA AAGGTCCCTGAAGTCTTCTCCCTGCCTGCCTGCCTGCTGGNGGGAAGTGGGGGCTGCTCT GNACTGAGTATGGAGACCGTGTATGGGACGNCACCTGTTTNNNGGACCGGGGGGAAGGCA NAACAAGGGATTCTTTT >OriGene 3' read for NM_199245 unedited
GGACGCGGCCGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTTAATGGTAGGAATTT GTATTTTTTGCCTTTGTTCAGATACATGACATTGGTAAATATGCCACATGCCTTTGGTGG AAGTACAACTGTTGTTATTACTCTATACAAGTATGAGATCAGGGTTAGGAAAAAAAGACA AAGAGGTGATGACAGACACACAGTGGAAACCCCACATCGTCTCATGGCAAACCGAAGAAC GGGATGTGGGAAGCTCAGCTTCATTTGACTGCAAAGTCCCAGGGTTTTGTTGCACATTTG CTCATGCACATGGGTGGTGGGAGGAAAGGGGGATAGCAAAGACACACAGAAGAGGGTACA GGGTGGGTGAGAAAGAAAGTAGAAGGGCTAATACCCCCAAAGAACAAGGCCAACTACACC TGGTGAGCCTCAGAGGGACAGAAACCCAGGAATGATTCCTGTGATAGGGCTGGGAGAGCC AAGAGGACGGATTCGCTCCAGGCTTGGGACACATCGAGGACAGTGGTGGTTCTTCTCCAG CGGTGACCCCCTGCATTAGGCAAGGAGGAGCCCAGAGGAGAGTGGAGACCTTCGAGGGGG GCCGTTGGGAGGGTACTGACTGCTTTCTTCCAGCTCTTCAGTCCCGCCCTTGGGCAGGAC GAAGGGAATGTGGGAAACAAGGGCGAAGGGAAAGGAATGGATGGTTTTGAGCAATGAAAT GCTGCTGCATGGAAAGTGGGCATCCAGACCCTGCCCAGCATGGCCTCAGCCTCTTTCTGT TCGTGGACCGAGGGGAAGATGGATTAAAGGGCCATGGGCATTCTNCGCTCTGCTCCAGNC TGCCCTTCTCCCCTGCACCTGGGCTATCNCACATAATAGCCATNCTGAGCTCACAATGCC CCGAGATAGCTGAGCAGTCCATTCTCAGGTTCACTGTCTTACCACATCTGCNAAGGGGGG ATAAAGGGGCAAGTNAATCAACGGAAAGGGGGTCAAGGGAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_199245 |
| Insert Size | 3420 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_199245.1, NP_954740.1 |
| RefSeq Size | 3151 bp |
| RefSeq ORF | 354 bp |
| Locus ID | 6843 |
| UniProt ID | P23763 |
| Protein Families | Secreted Protein, Transmembrane |
| Protein Pathways | SNARE interactions in vesicular transport |
| Gene Summary | Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2), also known as VAMP-1D, has a different 3' structure, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205125 | VAMP1 (Myc-DDK-tagged)-Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 2 |
CNY 1,200.00 |
|
| RC205125L3 | Lenti ORF clone of Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205125L4 | Lenti ORF clone of Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG205125 | VAMP1 (tGFP-tagged) - Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 2 |
CNY 2,800.00 |
