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PPP2R2D (NM_018461) Human Untagged Clone
CAT＃: SC128054
PPP2R2D (untagged)-Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | B55D; B55delta; MDS026 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_018461, the custom clone sequence may differ by one or more nucleotides
ATGGCAGGAGCCGGAGGCGGCGGCTGCCCCGCGGGCGGCAACGACTTCCAGTGGTGCTTCTCGCAGGTCA AGGGGGCCATCGACGAGGACGTGGCCGAAGCGGACATCATTTCCACCGTTGAGTTTAATTACTCTGGAGA TCTTCTTGCAACAGGAGACAAGGGCGGCAGAGTTGTTATTTTTCAGCGTGAACAAGAGAATAAAAGCCGC CCTCATTCTAGGGGAGAATATAATGTTTACAGCACCTTTCAAAGTCATGAACCGGAGTTTGACTATTTGA AAAGTCTAGAAATTGAGGAAAAAATTAATAAAATTAGGTGGTTACCACAACAGAATGCTGCTCATTTTCT ACTGTCTACAAATGATAAAACTATAAAATTATGGAAAATAAGTGAACGGGATAAAAGAGCAGAAGGTTAT AACCTGAAAGACGAAGATGGAAGACTTCGAGACCCATTTAGGATCACGGCGCTACGGGTCCCAATATTGA AGCCCATGGATCTTATGGTAGAAGCGAGTCCACGGCGAATTTTTGCAAATGCTCACACATATCATATAAA TTCCATTTCAGTAAATAGTGATCATGAAACATATCTTTCTGCAGATGACCTGAGAATTAATTTATGGCAC TTAGAAATCACAGATAGAAGCTTTAACATCGTGGACATCAAGCCTGCTAACATGGAGGAGCTGACCGAAG TCATCACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTGTTCGTCTACAGCAGTAGCAAAGGGACCAT CCGCCTGTGTGACATGCGCTCCTCGGCCCTGTGCGACAGACACTCCAAGTTTTTTGAAGAGCCTGAAGAT CCCAGCAGTAGGTCCTTCTTCTCAGAAATAATTTCATCCATATCCGATGTAAAATTCAGTCATAGTGGGC GGTACATGATGACCAGAGACTACCTGTCGGTGAAGGTGTGGGACCTCAACATGGAGAGCAGGCCGGTGGA GACCCACCAGGTCCACGAGTACCTGCGCAGCAAGCTCTGCTCTCTCTATGAGAACGACTGCATCTTTGAC AAGTTTGAGTGTTGCTGGAACGGTTCGGATAGCGCCATCATGACCGGGTCCTATAACAACTTCTTCAGGA TGTTTGATAGAGACACGCGGAGGGATGTGACCCTGGAGGCCTCGAGAGAGAGCAGCAAACCGCGCGCCAG CCTCAAACCCCGGAAGGTGTGTACGGGGGGTAAGCGGAGGAAAGACGAGATCAGTGTGGACAGTCTGGAC TTCAACAAGAAGATCCTGCACACAGCCTGGCACCCCGTGGACAATGTCATTGCCGTGGCTGCCACCAATA ACTTGTACATATTCCAGGACAAAATCAACTAG >OriGene 5' read for NM_018461 unedited
ACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCCCGGCGGCGGCGGCGGCGGCG GCGCCGGCGGTGGTGGCGGCCCCGGGGCTGAGCGCTCGGCTGCAGCGGCGCGGAGGCCGT CTCCCTGGTCTGCCGCGGTCCCCGCCCGTCCCGCCGCCGGCTGCCATGGCAGGAGCCGGA GGCGGCGGCTGCCCCGCGGGCGGCAACGACTTCCAGTGGTGCTTCTCGCAGGTCAAGGGG GCCATCGACGAGGACGTGGCCGAAGCGGACATCATTTCCACCGTTGAGTTTAATTACTCT GGAGATCTTCTTGCAACAGGAGACAAGGGCGGCAGAGTTGTTATTTTTCAGCGTGAACAA GAGAATAAAAGCCGCCCTCATTCTAGGGGAGAATATAATGTTTACAGCACCTTTCAAAGT CATGAACCGGAGTTTGACTATTTGAAAAGTCTAGAAATTGAGGAAAAAATTAATAAAATT AGGTGGTTACCACAACAGAATGCTGCTCATTTTCTACTGTCTACAAATGATAAAACTATA AAATTATGGAAAATAAGTGAACGGGATAAAAGAGCAGAAGGTTATAACCTGAAAGACGAA GATGGAAGACTTCGAGACCCATTTAGGATCACGGCGCTACGGGTCCCAATATTGAAGCCC ATGGATCTTATGGTAGAAGCGAGTCCACGGCGAATTTTTGCAAATGCTCACACATATCAT ATAAATTCCATTTCAGTAAATAGTGATCATGAAACATATCTTTCTGCAAATGACCTGAGA AATTAATTATGGCACTTAGAAATCACAGATAGAAGCTTTAACATCGGGGACATCAAGCCT GCTAACATGGAGGAGCTGACCGAAGTCATCACTGCAGCCGGAGTTCACCCGGACCAGTGC CACGTGTTCGTCTACAGCAAAGCAAAGT >Forward primer walk for NM_018461 unedited
NAAACAGCGAGCACGCGCGCCCGCCTCAACTCGGTAGTGTGTACGGGGGGTAAGCGGNAG GAAANACGAGCATCAGTGTGGACAGTCTGGACTTCAACAAAAAGATCCTGCACACAGCCT GGCACCCCGTGGACAATGTCATTGCCGTGGCTGCCACCAATAACTTGTACATATTCCAGG ACAAAATCAACTAGAGACGCGAACACACAGGAGAAAGCCGCCTCCGCTGGAGGCCCGGTG TGGTTCCGCCTCGGCGAGGCGCGAGACAGGCGCTGCTGCTCACGTGGAGACGCTCTCGAA GCAGAGTTGACGGACACTGCTCCCAAAAGCGTGGGGGTATGTGTGCNNCATTTCTGTNTC NNNNCTGCTNNTCCGTGNNTGGTCTTCCTTCGCACCCCCCCCACCACGGGAGAAGACCCC CAGAAACGACAACCCCCCAGCAGACAAACCCACCACCCACTAATTACCCCCCCCCCACAA CCGCACCGAACCAATTACACCGGTGCCAAACACCCCCCCCGCACAATACCTTAACAGCAC GCCACGCTAAACAGAAGCGAACTCCCACACATCAACACACCCCAAACCCACCCCCCCCAC TCGCCACGAATATGTCACCTTTTCCACCCCGACCAAAGAGAGCATATAACCCACCGGAGC GGCCTATCACCCCCCCGCCACATGCCGAACCGACACCCAACCGCCCAGTTTTTCAACACG AGTTCTCCCTTGTCGCACACCTTATATATTGAAATCCAAACACGAAGCAGCATGACCGCG GACACGTTAGCCGANCCACAACTAAAACACGCCTATGCACCCTAGTATCAAAACCCACCG AAATACGATGGATAACACGGGTTCCCACAAACAATAGACCAACGCGGTAATAAAAAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018461 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018461.2, NP_060931.2 |
| RefSeq Size | 2105 bp |
| RefSeq ORF | 1362 bp |
| Locus ID | 55844 |
| UniProt ID | Q66LE6 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Tight junction |
| Gene Summary | B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (a). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208230 | PPP2R2D (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1 |
CNY 3,656.00 |
|
| RC208230L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208230L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208230 | PPP2R2D (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1 |
CNY 5,256.00 |
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