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ATG5 (NM_004849) Human Untagged Clone
CAT＃: SC128244
ATG5 (untagged)-Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5)
CNY 3,600.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APG5; APG5-LIKE; APG5L; ASP; hAPG5; SCAR25 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC128244 sequence for NM_004849 edited (data generated by NextGen Sequencing)
ATGACAGATGACAAAGATGTGCTTCGAGATGTGTGGTTTGGACGAATTCCAACTTGTTTC ACGCTATATCAGGATGAGATAACTGAAAGGGAAGCAGAACCATACTATTTGCTTTTGCCA AGAGTAAGTTATTTGACGTTGGTAACTGACAAAGTGAAAAAGCACTTTCAGAAGGTTATG AGACAAGAAGACATTAGTGAGATATGGTTTGAATATGAAGGCACACCACTGAAATGGCAT TATCCAATTGGTTTGCTATTTGATCTTCTTGCATCAAGTTCAGCTCTTCCTTGGAACATC ACAGTACATTTTAAGAGTTTTCCAGAAAAAGACCTTCTGCACTGTCCATCTAAGGATGCA ATTGAAGCTCATTTTATGTCATGTATGAAAGAAGCTGATGCTTTAAAACATAAAAGTCAA GTAATCAATGAAATGCAGAAAAAAGATCACAAGCAACTCTGGATGGGATTGCAAAATGAC AGATTTGACCAGTTTTGGGCCATCAATCGGAAACTCATGGAATATCCTGCAGAAGAAAAT GGATTTCGTTATATCCCCTTTAGAATATATCAGACAACGACTGAAAGACCTTTCATTCAG AAGCTGTTTCGTCCTGTGGCTGCAGATGGACAGTTGCACACACTAGGAGATCTCCTCAAA GAAGTTTGTCCTTCTGCTATTGATCCTGAAGATGGGGAAAAAAAGAATCAAGTGATGATT CATGGAATTGAGCCAATGTTGGAAACACCTCTGCAGTGGCTGAGTGAACATCTGAGCTAC CCGGATAATTTTCTTCATATTAGTATCATCCCACAGCCAACAGATTGA Clone variation with respect to NM_004849.2 >OriGene 5' read for NM_004849 unedited
GGGGGGGGNGGGGGGGAANNNNNNTTTTTCNNNNNNNNNGGTCAGNATTTGTNATACGAC TCACTATAGGCGGCCGCGNAATTCGCACGAGGCTTCCTGGNATCCTGCTACCGCGTCCCC GCAGAACAGTGTGTCAGGCGGGCAGCTTGCCCCGCCGCCCCACCGGAGCGCGGAATCTGG GCGTCCCCACCAGTGCGGGGAGCCGGAAGGAGGAGCCATAGCTTGGAGTAGGTTTGGCTT TGGTTGAAATAAGAATTTAGCCTGTATGTACTGCTTTAACTCCTGGAAGAATGACAGATG ACAAAGATGTGCTTCGAGATGTGTGGTTTGGACGAATTCCAACTTGTTTCACGCTATATC AGGATGAGATAACTGAAAGGGAAGCAGAACCATACTATTTGCTTTTGCCAAGAGTAAGTT ATTTGACGTTGGTAACTGACAAAGTGAAAAAGCACTTTCAGAAGGTTATGAGACAAGAAG ACATTAGTGAGATATGGTTTGAATATGAAGGCACACCACTGAAATGGCATTATCCAATTG GTTTGCTATTTGATCTTCTTGCATCAAGTTCAGCTCTTCCTTGGAACATCACAGTACATT TTAAGAGTTTTCCAGAAAAAGACCTTCTGCACTGTCCATCTAAGGATGCAATTGAAGCTC ATTTTATGTCATGTATGAAAGAAGCTGATGCTTTAAAACATAAAAGTCAAGTAATCAATG AAATGCAGAANAAAGATCACAAGCAACTCTGGATGGGATTGCAAAATGACAGATTTGACC AGTTTTGGGCCATCAATCGNAAACTCATGGNATATCCTGCAGAAGAAAATGGATTTCGTT ATATCCCCTTTAGATATATCANACNACGACTGANAGACCTTTCATTCAGAAGCTGTTTCG TCCTGTGGCTGCANATGGCAGTTGCCACACTAN >Forward primer walk for NM_004849 unedited
NAAGGTTCATTTNAAAATTTTCCANAAAAAACCTCTGCCTGTCCACTAAGGATGCAATTG AAGCTCATTTTATGTCATGTATGAAAGAAGCTGATGCTTTAAAACATAAAAGTCAAGTAA TCAATGAAATGCAGAAAAAAGATCACAAGCAACTCTGGATGGGATTGCAAAATGACAGAT TTGACCAGTTTTGGGCCATCAATCGGAAACTCATGGAATATCCTGCAGAAGAAAATGGAT TTCGTTATATCCCCTTTAGAATATATCAGACAACGACTGAAAGACCTTTCATTCAGAAGC TGTTTCGTCCTGTGGCTGCAGATGGACAGTTGCACACACTAGGAGATCTCCTCAAAGAAG TTTGTCCTTCTGCTATTGATCCTGAAGATGGGGAAAAAAAGAATCAAGTGATGATTCATG GAATTGAGCCAATGTTGGAAACACCTCTGCAGTGGCTGAGTGAACATCTGAGCTACCCGG ATAATTTTCTTCATATTAGTATCATCCCACAGCCAACAGATTGAAGGATCAACTATTTGC CTGAACAGAATCATCCTTAAATGGGATTTATCAGAGCATGTCACCCTTTTGCTTCAATCA GGTTTGGTGGAGGCAACCTGACCAGAAACACTTCGCTGCTGCAAGCCAGACAGGAAAAAG ATTCCATGTCAGATAAGGCAACTGGGCTGGTCTTACTTTGCATCACCTCTGCTTTCCTCC ACTGCCATCATTAAACCTCAGCTGTGACATGAAAGACTTACCGGACCACTNGAAGGTCTT CTGNTAAATATAATGAAGCTGANACCTTTGGCCTAAGAAGAAATGGNAGTATGTGCCACT CGATTTGTATTTCTGATTAAAAAATAACAGGGGTATTTCCTAANGTGACCATGGNTTGAA CTTAGCTCATG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004849 |
| Insert Size | 3400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004849.2, NP_004840.1 |
| RefSeq Size | 3244 bp |
| RefSeq ORF | 828 bp |
| Locus ID | 9474 |
| UniProt ID | Q9H1Y0 |
| Domains | APG5 |
| Protein Families | Druggable Genome |
| Protein Pathways | Regulation of autophagy, RIG-I-like receptor signaling pathway |
| Gene Summary | The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Both variants 1 and 2 encode isoform a. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Novel protein complexes containing autophagy and UPS components regulate proteasome-dependent PARK2 recruitment onto mitochondria and PARK2-PARK6 activity during mitophagy
,null,
Cell Death & Disease
,PubMed ID 36357363
[ATG5]
|
|
Tumor heterogeneity in autophagy-dependent ferroptosis
,Li, J;Liu, J;Xu, Y;Wu, R;Chen, X;Song, X;Zeh, H;Kang, R;Klionsky, DJ;Wang, X;Tang, D;,
Autophagy
,PubMed ID 33404288
[ATG5]
|
|
Impaired Autophagy of an Intracellular Pathogen Induced by a Crohn's Disease Associated ATG16L1 Variant
,Petric Kuballa, Alan Huett, John D. Rioux, Mark J. Daly, Ramnik J. Xavier,
PLoS ONE 3(10): e3391. doi:10.1371/journal.pone.0003391
[ATG5]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210563 | ATG5 (Myc-DDK-tagged)-Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5) |
CNY 3,600.00 |
|
| RC210563L1 | Lenti ORF clone of Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC210563L2 | Lenti ORF clone of Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5), mGFP tagged |
CNY 6,000.00 |
|
| RC210563L3 | Lenti ORF clone of Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC210563L4 | Lenti ORF clone of Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5), mGFP tagged |
CNY 6,000.00 |
|
| RG210563 | ATG5 (tGFP-tagged) - Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5) |
CNY 5,200.00 |
