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BRSK1 (NM_032430) Human Untagged Clone
CAT＃: SC305447
BRSK1 (untagged)-Human BR serine/threonine kinase 1 (BRSK1)
CNY 15,010.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hSAD1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_032430 edited
GGCACCATGTCGTCCGGGGCCAAGGAGGGAGGTGGGGGCTCTCCCGCCTACCACCTCCCC CACCCCCACCCCCACCCACCCCAGCACGCCCAATATGTGGGCCCCTATCGGCTGGAGAAG ACGCTGGGCAAAGGACAGACAGGGCTGGTTAAACTCGGGGTCCACTGCATCACGGGTCAG AAGGTCGCCATCAAGATCGTGAACCGGGAGAAGCTGTCGGAGTCGGTGCTGATGAAGGTG GAGCGGGAGATCGCCATCCTGAAGCTCATCGAACACCCACATGTCCTCAAGCTCCACGAC GTCTACGAGAACAAGAAATATTTGTACCTGGTTCTGGAGCACGTCTCGGGGGGTGAGCTA TTCGACTACCTGGTAAAGAAGGGGAGACTGACGCCCAAGGAGGCCCGAAAGTTCTTCCGC CAGATTGTGTCTGCGCTGGACTTCTGCCACAGCTACTCCATCTGCCACAGAGACCTAAAG CCCGAGAACCTGCTTTTGGATGAGAAAAACAACATCCGCATTGCAGACTTCGGCATGGCG TCCCTGCAGGTGGGGGACAGCCTCCTGGAGACCAGCTGCGGGTCCCCCCATTATGCGTGT CCAGAGGTGATTAAGGGGGAAAAATATGATGGCCGCCGGGCAGACATGTGGAGCTGTGGA GTCATCCTCTTCGCCCTGCTCGTGGGGGCTCTGCCCTTTGATGACGACAACCTCCGCCAG CTGCTGGAGAAGGTGAAACGGGGCGTCTTCCACATGCCCCACTTCATTCCTCCAGATTGC CAGAGCCTCCTGAGGGGAATGATCGAAGTGGAGCCCGAAAAAAGGCTCAGTCTGGAGCAA ATTCAGAAACATCCTTGGTACCTAGGCGGGAAACACGAGCCAGACCCGTGCCTGGAGCCA GCCCCTGGCCGCCGGGTAGCCATGCGGAGCCTGCCATCCAACGGAGAGCTGGACCCCGAC GTCCTAGAGAGCATGGCATCACTGGGCTGCTTCAGGGACCGCGAGAGGCTGCATCGCGAG CTGCGCAGTGAGGAGGAGAACCAAGAAAAGATGATATATTATCTGCTTTTGGATCGGAAG GAGCGGTATCCCAGCTGTGAGGACCAGGACCTGCCTCCCCGGAATGATGTTGACCCCCCC CGGAAGCGTGTGGATTCTCCCATGCTGAGCCGTCACGGGAAGCGGCGACCAGAGCGGAAG TCCATGGAAGTCCTGAGCATCACCGATGCCGGGGGTGGTGGCTCCCCTGTACCCACCCGA CGGGCCTTGGAGATGGCCCAGCACAGCCAGAGATCCCGTAGCGTCAGTGGAGCCTCCACG GGTCTGTCCTCCAGCCCTCTAAGCAGCCCAAGGAGTCCGGTCTTTTCCTTTTCACCGGAG CCGGGGGCTGGAGATGAGGCTCGAGGCGGGGGCTCCCCGACTTCCAAAACGCAGACGCTG CCTTCTCGGGGCCCCAGGGGTGGGGGCGCCGGGGAGCAGCCCCCGCCCCCCAGTGCCCGC TCCACACCCCTGCCCGGCCCCCCAGGCTCCCCGCGCTCCTCTGGCGGGACCCCCTTGCAC TCGCCTCTGCACACGCCCCGGGCCAGTCCCACCGGGACCCCGGGGACAACACCACCCCCC AGCCCCGGCGGTGGCGTCGGGGGAGCCGCCTGGAGGAGTCGTCTCAACTCCATCCGCAAC AGCTTCCTGGGCTCCCCTCGCTTTCACCGGCGCAAGATGCAGGTCCCTACCGCTGAGGAG ATGTCCAGCTTGACGCCAGAGTCCTCCCCGGAGCTGGCAAAACGCTCCTGGTTCGGGAAC TTCATCTCCTTGGACAAAGAAGAACAAATATTCCTCGTGCTAAAGGACAAACCTCTCAGC AGCATCAAAGCAGACATCGTCCATGCCTTTCTGTCGATCCCCAGCCTGAGTCACAGTGTG CTGTCACAGACCAGCTTCAGGGCCGAGTACAAGGCCAGTGGCGGCCCCTCCGTCTTCCAA AAGCCCGTCCGCTTCCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAGCCCTCCCCGCGA CGGGACGGCAGCGGAGGTGGTGGCATCTACTCCGTCACCTTCACTCTCATCTCGGGTCCC AGCCGTCGGTTCAAGCGAGTGGTGGAGACCATCCAGGCACAGCTCCTGAGCACTCATGAC CAGCCCTCCGTGCAGGCCCTGGCAGACGAGAAGAACGGGGCCCAGACCCGGCCTGCTGGT GCCCCACCCCGAAGCCTGCAGCCCCCACCCGGCCGCCCAGACCCAGAGCTGAGCAGCTCT CCCCGCCGAGGCCCCCCCAAGGACAAGAAGCTCCTGGCCACCAACGGGACCCCTCTGCCC TGACCCCACGGGGCCGGGGAGGGAGGGGACCCCCCTCCACCCCCCTTCCGTGCCCCCCAA CTGTGAATCTGTAAATAAGGCCCAAGGAACATGTCGGGAGGGGGGTGGACACAAAAACCG GCCTTGCCCTGCAGGGATGGGGCTCCACAGGCCGTGCCCAACTGGGGGTGGTTCTAGGGG AACAGGGGGCGGGGGAGCTGTTTCTATTTTATTTATTGATTAATTTATTATTTTATTTAT TGATCAATCTCTCTGCGGGGTGGGGTGGGGGAGGGACGGGAGCTGGTTGGGGTGGCTTAG CAGATCCGGACAGGGCCCTCTGTCCCTGTGTCGTCCCCAACCCCCTCTTCCCGGGCCCCT CCTCCCCTGGTCCTCCCCCCACGACCTTCTGTACGGATTTGCTCTCCGGAAGGAATTCTG GTTTCGCGTGATCCTGCCTGCGTCCGTGTCTCTGATTCCGCCGGCGGCAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAGATAATAATAATAAATAGCCTTGATCAGGGAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_032430 |
| Insert Size | 2900 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to the protein associated with this reference, NM_032430.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032430.1, NP_115806.1 |
| RefSeq Size | 3109 bp |
| RefSeq ORF | 2337 bp |
| Locus ID | 84446 |
| UniProt ID | Q8TDC3 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211767 | BRSK1 (Myc-DDK-tagged)-Human BR serine/threonine kinase 1 (BRSK1) |
CNY 8,544.00 |
|
| RC211767L1 | Lenti-ORF clone of BRSK1 (Myc-DDK-tagged)-Human BR serine/threonine kinase 1 (BRSK1) |
CNY 10,944.00 |
|
| RC211767L2 | Lenti-ORF clone of BRSK1 (mGFP-tagged)-Human BR serine/threonine kinase 1 (BRSK1) |
CNY 10,070.00 |
|
| RC211767L3 | Lenti-ORF clone of BRSK1 (Myc-DDK-tagged)-Human BR serine/threonine kinase 1 (BRSK1) |
CNY 10,070.00 |
|
| RC211767L4 | Lenti-ORF clone of BRSK1 (mGFP-tagged)-Human BR serine/threonine kinase 1 (BRSK1) |
CNY 10,070.00 |
|
| RG211767 | BRSK1 (tGFP-tagged) - Human BR serine/threonine kinase 1 (BRSK1) |
CNY 9,030.00 |
