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DEPDC5 (NM_014662) Human Untagged Clone
CAT＃: SC308914
DEPDC5 (untagged)-Human DEP domain containing 5 (DEPDC5), transcript variant 1
CNY 10,450.00
货期*
7周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEP.5; FFEVF; FFEVF1 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_014662, the custom clone sequence may differ by one or more nucleotides
ATGAGAACAACAAAGGTCTACAAACTCGTCATCCACAAGAAGGGCTTTGGGGGCAGTGAT GATGAGCTAGTTGTGAACCCCAAAGTGTTCCCTCACATCAAGCTTGGAGACATTGTAGAG ATTGCACACCCCAACGATGAATACAGCCCTCTGCTTTTGCAGGTCAAGTCTCTTAAGGAA GATTTACAGAAGGAAACTATCAGTGTGGACCAGACTGTGACTCAAGTGTTCCGGCTGAGA CCTTATCAGGATGTCTATGTTAATGTCGTAGACCCTAAGGATGTGACCCTTGACCTAGTG GAATTAACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACTAAAGAAAAGT TTGGTCAGCACATGTGCCTATATCACCCAGAAGGTGGAGTTTGCTGGCATCAGAGCACAG GCTGGTGAACTGTGGGTTAAGAATGAGAAGGTCATGTGTGGCTACATCAGTGAAGATACC AGGGTGGTGTTTCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAA ATGTGGGATTTTGATATTTATGGGGATTTGTATTTTGAGAAAGCTGTGAATGGTTTCCTT GCTGATCTATTTACCAAGTGGAAGGAGAAGAACTGTAGTCATGAAGTGACAGTGGTCCTG TTTTCTAGAACTTTCTATGATGCAAAATCTGTTGATGAATTTCCTGAAATAAACCGAGCC TCAATTCGACAGGATCACAAGGGGAGATTCTATGAAGACTTTTACAAAGTGGTGGTGCAG AATGAGAGAAGAGAAGAATGGACTTCACTTCTCGTAACCATTAAAAAACTCTTCATCCAG TATCCAGTGTTGGTGCGACTGGAACAGGCAGAGGGCTTTCCTCAAGGAGATAATTCTACC TCAGCACAAGGAAACTACCTGGAGGCCATCAATCTGTCATTCAATGTGTTTGATAAGCAC TACATCAACCGCAACTTTGACCGAACTGGGCAGATGTCAGTGGTGATCACGCCCGGGGTG GGTGTCTTTGAAGTGGACCGCCTACTCATGATCCTGACCAAGCAGCGGATGATAGATAAT GGAATTGGTGTGGATTTGGTGTGCATGGGAGAGCAACCGTTACATGCTGTCCCATTGTTC AAGCTCCATAATCGGAGTGCTCCCCGTGATTCTCGTCTGGGCGATGACTATAATATCCCT CACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAATAGTTTCACC CCACGAATAAAACTGGCAGGAAAGAAGCCCGCCTCTGAGAAAGCAAAAAATGGCCGTGAT ACATCTCTCGGGAGTCCAAAAGAATCTGAGAACGCCCTTCCCATCCAAGTAGATTATGAC GCCTATGACGCTCAAGTGTTCAGGCTGCCCGGCCCATCCCGGGCCCAGTGCCTCACCACC TGCAGATCTGTGCGAGAGCGAGAGAGTCACAGTCGAAAGAGTGCCAGCTCCTGTGATGTT TCATCCAGCCCTTCCCTACCAAGCCGCACACTGCCCACTGAGGAAGTGAGGAGCCAGGCT TCTGACGACAGCTCCCTAGGCAAGAGTGCCAACATCCTGATGATCCCACACCCCCACCTG CACCAGTATGAAGTCAGCAGCTCCTTGGGATACACCAGCACTCGAGATGTCCTGGAGAAC ATGATGGAGCCACCACAGCGAGACTCCAGTGCACCAGGGAGGTTTCACGTTGGCAGTGCA GAATCCATGCTGCATGTTCGACCTGGTGGATACACGCCCCAGAGAGCACTGATTAACCCC TTCGCTCCCTCTCGGATGCCCATGAAGCTTACGTCCAACAGAAGGCGCTGGATGCACACT TTTCCTGTGGGGCCATCCGGAGAAGCCATCCAGATCCACCACCAGACCCGACAGAATATG GCGGAGCTACAAGGCAGCGGGCAGAGGGATCCAACTCACTCCTCTGCAGAGCTGCTGGAG TTAGCATATCATGAAGCTGCTGGAAGGCACAGCAATTCCCGCCAGCCTGGTGACGGCATG TCCTTCTTGAACTTCAGTGGAACAGAGGAGCTTTCTGTCGGCCTGCTTAGCAACAGTGGT GCAGGTATGAATCCTAGGACCCAGAATAAGGATTCTCTAGAGGACAGTGTTTCTACCTCT CCAGACCCAATGCCAGGCTTCTGTTGCACAGTTGGAGTGGACTGGAAGTCTCTCACTACT CCGGCGTGCCTCCCCCTTACCACCGACTACTTCCCTGACCGCCAGGGCCTGCAGAATGAC TACACAGAGGGCTGTTATGATCTCCTTCCAGAAGCAGACATCGACAGGAGGGACGAAGAT GGTGTGCAGATGACAGCCCAGCAGGTATTTGAAGAGTTTATTTGCCAACGTCTCATGCAG GGCTACCAAATCATAGTGCAGCCCAAGACACAGAAACCCAATCCTGCTGTCCCGCCCCCG CTGAGCAGTAGCCCACTCTATAGCCGAGGCCTTGTGTCCCGAAACCGCCCTGAGGAGGAG GACCAGTATTGGCTGAGTATGGGCAGAACGTTCCACAAAGTGACGCTGAAGGATAAGATG ATCACAGTGACGCGATACCTTCCCAAGTATCCTTATGAATCTGCCCAGATCCACTACACC TACAGCCTCTGTCCTTCCCACTCAGACTCAGAGTTCGTCTCCTGCTGGGTGGAATTCTCC CACGAACGGCTGGAGGAGTACAAGTGGAATTACTTAGATCAGTATATCTGTTCTGCCGGC TCTGAAGACTTCAGCTTAATTGAGTCCCTGAAGTTCTGGAGGACCCGCTTCCTGCTGCTG CCAGCCTGTGTCACCGCCACCAAGCGCATCACGGAGGGGGAGGCCCACTGCGACATCTAT GGGGACAGGCCCCGTGCAGACGAGGACGAGTGGCAACTCCTGGATGGTTTTGTCCGCTTT GTGGAGGGCTTGAATCGCATTCGCAGGCGGCATCGCTCGGATCGCATGATGCGGAAAGGG ACCGCCATGAAAGGCTTGCAGATGACTGGGCCCATTTCCACGCATTCTCTGGAGTCAACT GCACCCCCAGTGGGGAAGAAGGGAACCTCAGCTCTCTCTGCCCTGTTGGAGATGGAGGCC AGTCAGAAGTGCCTGGGAGAACAGCAGGCAGCTGTGCATGGTGGGAAGAGCTCCGCCCAG TCAGCCGAGAGCAGCAGCGTTGCCATGACTCCCACCTACATGGACAGCCCACGAAAGGTA TCTGTGGACCAAACAGCCACTCCTATGTTGGACGGCACCAGTTTGGGCATATGCACAGGC CAATCCATGGACAGAGGCAACAGCCAGACCTTTGGGAACTCCCAGAACATAGGAGAACAG GGCTACTCCTCCACAAACTCCAGTGACAGCAGCTCTCAGCAGCTGGTGGCAAGCTCCTTG ACCTCATCCTCTACCCTGACAGAGATCCTGGAAGCCATGAAGCACCCCTCGACAGGAGTC CAGCTGCTCTCTGAACAGAAGGGCCTCTCACCGTACTGCTTCATCAGCGCGGAGGTGGTA CACTGGTTGGTGAACCACGTGGAGGGGATCCAGACACAGGCGATGGCCATTGACATCATG CAGAAAATGCTGGAAGAGCAGCTCATCACACATGCATCTGGCGAAGCCTGGCGGACCTTC ATCTACGGCTTCTATTTCTACAAGATAGTAACGGACAAAGAGCCCGACCGAGTGGCCATG CAGCAGCCCGCCACCACCTGGCACACAGCAGGAGTGGACGACTTCGCCAGCTTCCAGCGC AAGTGGTTTGAGGTGGCCTTTGTGGCAGAAGAGCTCGTGCACTCTGAGATTCCTGCCTTT CTCCTGCCCTGGCTGCCTAGCCGGCCAGCCTCCTATGCAAGTAGGCACAGCTCCTTTAGC CGAAGTTTTGGAGGACGGAGCCAGGCGGCAGCACTTTTAGCTGCCACTGTCCCAGAGCAG AGGACTGTGACCCTGGATGTTGACGTGAACAACCGCACAGACCGGCTGGAGTGGTGCAGC TGTTATTACCATGGCAACTTTTCTCTGAATGCAGCCTTTGAGATCAAGCTGCACTGGATG GCGGTGACCGCAGCAGTACTCTTCGAGATGGTCCAAGGTTGGCATCGGAAAGCCACCTCC TGTGGCTTCTTGTTAGTCCCAGTTTTGGAGGGGCCTTTTGCACTGCCCAGTTACCTGTAT GGCGACCCCCTTCGTGCCCAGCTCTTCATCCCACTCAACATCAGCTGCTTGCTCAAGGAG GGCAGCGAGCACCTGTTTGATAGCTTTGAACCCGAAACGTACTGGGATCGAATGCACCTC TTCCAGGAAGCCATTGCACACAGGTTTGGGTTTGTACAAGATAAATATTCTGCCTCTGCT TTTAACTTCCCTGCTGAGAACAAGCCTCAGTATATCCACGTTACAGGAACAGTGTTTCTG CAGCTGCCCTACTCCAAGCGCAAGTTCTCAGGGCAGCAGCGGCGGCGGCGGAACTCCACC AGCTCCACCAACCAGAACATGTTCTGCGAGGAGCGGGTCGGCTACAACTGGGCCTACAAC ACCATGCTCACCAAAACATGGCGCTCCAGCGCCACAGGGGATGAAAAGTTTGCTGATCGG CTGCTGAAGGACTTCACGGACTTCTGCATCAACCGTGACAACCGGCTGGTCACGTTCTGG ACAAGTTGCCTGGAGAAGATGCATGCCAGTGCCCCGTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_014662 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014662.2, NP_055477.1 |
| RefSeq Size | 5394 bp |
| RefSeq ORF | 5316 bp |
| Locus ID | 9681 |
| UniProt ID | O75140 |
| Domains | DEP |
| Gene Summary | This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (1) uses an alternate in-frame splice site in an internal exon and lacks an in-frame exon in the 3' coding region compared to variant 4. The encoded protein (isoform 1) is shorter than isoform 4. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221026 | DEPDC5 (Myc-DDK-tagged)-Human DEP domain containing 5 (DEPDC5), transcript variant 1 |
CNY 15,680.00 |
|
| RC221026L3 | Lenti-ORF clone of DEPDC5 (Myc-DDK-tagged)-Human DEP domain containing 5 (DEPDC5), transcript variant 1 |
CNY 17,580.00 |
|
| RC221026L4 | Lenti-ORF clone of DEPDC5 (mGFP-tagged)-Human DEP domain containing 5 (DEPDC5), transcript variant 1 |
CNY 17,580.00 |
|
| RG221026 | DEPDC5 (tGFP-tagged) - Human DEP domain containing 5 (DEPDC5), transcript variant 1 |
CNY 17,290.00 |
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