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NEK1 (NM_012224) Human Untagged Clone
CAT＃: SC309937
NEK1 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2
CNY 24,230.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALS24; NY-REN-55; SRPS2; SRPS2A; SRTD6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309937 representing NM_012224.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGAAGTATGTTAGACTACAGAAGATTGGAGAAGGTTCATTTGGAAAAGCCATTCTTGTTAAATCT ACAGAAGATGGCAGACAGTATGTTATCAAGGAAATTAACATCTCAAGAATGTCCAGTAAAGAAAGAGAA GAATCAAGGAGAGAAGTTGCAGTATTGGCAAACATGAAGCATCCAAATATTGTCCAGTATAGAGAATCA TTTGAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAGGGAGGGGATCTGTTTAAGCGAATA AATGCTCAGAAAGGCGTTTTGTTTCAAGAGGATCAGATTTTGGACTGGTTTGTACAGATATGTTTGGCC CTGAAACATGTACATGATAGAAAAATTCTTCATCGAGACATTAAATCTCAGAACATATTTTTAACTAAA GATGGAACAGTACAACTTGGAGATTTTGGAATTGCTAGAGTTCTTAATAGTACTGTAGAGCTGGCTCGA ACTTGCATAGGGACCCCATACTACTTGTCACCTGAAATCTGTGAAAACAAACCTTACAATAATAAAAGT GACATTTGGGCTCTGGGGTGTGTCCTTTATGAGCTGTGTACACTTAAACATGCTTTTGAAGCTGGCAGT ATGAAAAACCTGGTACTGAAGATAATATCTGGATCTTTTCCACCTGTGTCTTTGCATTATTCCTATGAT CTCCGCAGTTTGGTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTG GAGAAAGGTTTTATAGCCAAACGCATTGAAAAGTTTCTCTCTCCTCAGCTTATTGCAGAAGAATTTTGT CTAAAAACATTTTCGAAGTTTGGATCACAGCCTATACCAGCTAAAAGACCAGCTTCAGGACAAAACTCG ATTTCTGTTATGCCTGCTCAGAAAATTACAAAGCCTGCCGCTAAATATGGAATACCTTTAGCATATAAG AAATATGGAGATAAAAAATTACACGAAAAGAAACCACTGCAAAAACATAAACAGGCCCATCAAACTCCA GAGAAGAGAGTGAATACTGGAGAAGAAAGGAGGAAAATATCTGAGGAAGCAGCAAGAAAGAGAAGGCTG GAATTTATTGAAAAAGAAAAGAAACAAAAGGATCAGATTATTAGTTTAATGAAGGCTGAACAAATGAAA AGGCAAGAAAAGGAAAGGTTGGAAAGAATAAATAGGGCCAGGGAACAAGGATGGAGAAATGTGCTAAGT GCTGGTGGAAGTGGTGAAGTAAAGGCTCCTTTTCTGGGCAGTGGAGGGACTATAGCTCCATCATCTTTT TCTTCTCGAGGACAGTATGAACATTACCATGCCATTTTTGACCAAATGCAGCAACAAAGAGCAGAAGAT AATGAAGCTAAATGGAAAAGAGAAATATATGGTCGAGGTCTTCCAGAAAGAGGAATTCTGCCTGGAGTT CGTCCAGGATTTCCTTATGGGGCTGCAGGTCATCACCATTTTCCTGATGCTGATGATATTAGAAAAACT TTGAAAAGATTGAAGGCGGTGTCTAAACAAGCCAATGCAAACAGGCAAAAAGGGCAGCTAGCTGTAGAA AGAGCTAAACAAGTAGAAGAGTTCCTGCAGCGAAAACGGGAAGCTATGCAGAATAAAGCTCGAGCCGAA GGACATATGGTTTATCTGGCAAGACTGAGGCAAATAAGACTACAGAATTTCAATGAGCGCCAACAGATT AAAGCCAAACTTCGTGGTGAAAAGAAAGAAGCTAATCATTCTGAAGGACAAGAAGGAAGTGAAGAGGCT GACATGAGGCGCAAAAAAATCGAATCACTGAAGGCCCATGCAAATGCACGTGCTGCTGTACTAAAAGAA CAACTAGAACGAAAGAGAAAGGAGGCTTATGAGAGAGAAAAAAAAGTGTGGGAAGAGCATTTGGTGGCT AAAGGAGTTAAGAGTTCTGATGTTTCTCCACCTTTGGGACAGCATGAAACAGGTGGCTCTCCATCAAAG CAACAGATGAGATCTGTTATTTCTGTAACTTCAGCTTTGAAAGAAGTTGGCGTGGACAGTAGTTTAACT GATACCCGGGAAACTTCAGAAGAGATGCAAAAGACCAACAATGCTATTTCAAGTAAGCGAGAAATACTT CGTAGATTAAATGAAAATCTTAAAGCTCAAGAAGATGAAAAAGGAAAGCAGAATCTCTCTGATACTTTT GAGATAAATGTTCATGAAGATGCCAAAGAGCATGAAAAAGAAAAATCAGTTTCATCTGATCGCAAGAAG TGGGAGGCAGGAGGTCAACTTGTGATTCCTCTGGATGAGTTAACACTAGATACATCCTTCTCTACAACT GAAAGACATACAGTGGGAGAAGTTATTAAATTAGGTCCTAATGGATCTCCAAGAAGAGCCTGGGGGAAA AGTCCGACAGATTCTGTTCTAAAGATACTTGGAGAAGCTGAACTACAACTTCAGACAGAACTATTAGAA AATACAACTATTAGAAGTGAGATTTCTCCCGAAGGGGAAAAGTACAAACCCTTAATTACTGGAGAAAAA AAAGTACAATGTATTTCACATGAAATAAACCCATCAGCTATTGTTGATTCTCCTGTTGAGACAAAAAGT CCCGAGTTCAGTGAGGCATCTCCACAGATGTCATTGAAACTGGAAGGAAATTTAGAAGAACCTGATGAT TTGGAAACAGAAATTCTACAAGAGCCAAGTGGAACAAACAAAGATGAGAGCTTGCCATGCACTATTACT GATGTGTGGATTAGTGAGGAAAAAGAAACAAAGGAAACTCAGTCGGCAGATAGGATCACCATTCAGGAA AATGAAGTTTCTGAAGATGGAGTCTCGAGTACTGTGGACCAACTTAGTGACATTCATATAGAGCCTGGA ACCAATGATTCTCAGCACTCTAAATGTGATGTAGATAAGTCTGTGCAACCGGAACCATTTTTCCATAAG GTGGTTCATTCTGAACACTTGAACTTAGTCCCTCAAGTTCAATCAGTTCAGTGTTCACCAGAAGAATCC TTTGCATTTCGATCTCACTCGCATTTACCACCAAAAAATAAAAACAAGAATTCCTTGCTGATTGGACTT TCAACTGGTCTGTTTGATGCAAACAACCCAAAGATGTTAAGGACATGTTCACTTCCAGATCTCTCAAAG CTGTTCAGAACCCTTATGGATGTTCCCACCGTAGGAGATGTTCGTCAAGACAATCTTGAAATAGATGAA ATTGAAGATGAAAACATTAAAGAAGGACCTTCTGATTCTGAAGACATTGTGTTTGAAGAAACTGACACA GATTTACAAGAGCTGCAGGCCTCGATGGAACAGTTACTTAGGGAACAACCTGGTGAAGAATACAGTGAA GAAGAAGAGTCAGTCTTGAAGAACAGTGATGTGGAGCCAACTGCAAATGGGACAGATGTGGCAGATGAA GATGACAATCCCAGCAGTGAAAGTGCCCTGAACGAAGAATGGCACTCAGATAACAGTGATGGTGAAATT GCTAGTGAATGTGAATGCGATAGTGTCTTTAACCATTTAGAGGAACTGAGACTTCATCTGGAGCAGGAA ATGGGCTTTGAAAAATTCTTTGAGGTTTATGAGAAAATAAAGGCTATTCATGAAGATGAAGATGAAAAT ATTGAAATTTGTTCAAAAATAGTTCAAAATATTTTGGGAAATGAACATCAGCATCTTTATGCCAAGATT CTTCATTTAGTCATGGCAGATGGAGCCTACCAAGAAGATAATGATGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012224 |
| Insert Size | 3777 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012224.2 |
| RefSeq Size | 5689 bp |
| RefSeq ORF | 3777 bp |
| Locus ID | 4750 |
| UniProt ID | Q96PY6 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 142.8 kDa |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221729 | NEK1 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2 |
CNY 8,760.00 |
|
| RC221729L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, Myc-DDK-tagged |
CNY 11,160.00 |
|
| RC221729L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, mGFP tagged |
CNY 11,500.00 |
|
| RC221729L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC221729L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, mGFP tagged |
CNY 11,500.00 |
|
| RG221729 | NEK1 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2 |
CNY 10,360.00 |
|
| SC323341 | NEK1 (untagged)-Kinase deficient mutant (K33M) of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1) |
CNY 8,768.00 |
