NEK1 (NM_012224) Human Untagged Clone
CAT#: SC323341
NEK1 (untagged)-Kinase deficient mutant (K33M) of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1)
CNY 8,768.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ALS24; NY-REN-55; SRPS2; SRPS2A; SRTD6 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC323341 sequence for NM_012224 edited (data generated by NextGen Sequencing)
ATGGAGAAGTATGTTAGACTACAGAAGATTGGAGAAGGTTCATTTGGAAAAGCCATTCTT GTTAAATCTACAGAAGATGGCAGACAGTATGTTATCATGGAAATTAACATCTCAAGAATG TCCAGTAAAGAAAGAGAAGAATCAAGGAGAGAAGTTGCAGTATTGGCAAACATGAAGAAT CCAAATATTGTCCAGTATAGAGAATCATTTGAAGAAAATGGCTCTCTCTACATAGTAATG GATTACTGTGAGGGAGGGGATCTGTTTAAGCGAATAAATGCTCAGAAAGGCGTTTTGTTT CAAGAGGATCAGATTTTGGACTGGTTTGTACAGATATGTTTGGCCCTGAAACATGTACAT GATAGAAAAATTCTTCATCGAGACATTAAATCTCAGAACATATTTTTAACTAAAGATGGA ACAGTACAACTTGGAGATTTTGGAATTGCTAGAGTTCTTAATAGTACTGTAGAGCTGGCT CGAACTTGCATAGGGACCCCATACTACTTGTCACCTGAAATCTGTGAAAACAAACCTTAC AATAATAAAAGTGACATTTGGGCTCTGGGGTGTGTCCTTTATGAGCTGTGTACACTTAAA CATGCTTTTGAAGCTGGCAGTATGAAAAACCTGGTACTGAAGATAATATCTGGATCTTTT CCACCTGTGTCTTTGCATTATTCCTATGATCTCCGCAGTTTGGTGTCTCAGTTATTTAAA AGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTTTATAGCCAAA CGCATTGAAAAGTTTCTCTCTCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACATTT TCGAAGTTTGGATCACAGCCTATACCAGCTAAAAGACCAGCTTCAGGACAAAACTCGATT TCTGTTATGCCTGCTCAGAAAATTACAAAGCCTGCCGCTAAATATGGAATACCTTTAGCA TATAAGAAATATGGAGATAAAAAATTACACGAAAAGAAACCACTGCAAAAACATAAACAG GCCCATCAAACTCCAGAGAAGAGAGTGAATACTGGAGAAGAAAGGAGGAAAATATCTGAG GAAGCAGCAAGAAAGAGAAGGCTGGAATTTATTGAAAAAGAAAAGAAACAAAAGGATCAG ATTATTAGTTTAATGAAGGCTGAACAAATGAAAAGGCAAGAAAAGGAAAGGTTGGAAAGA ATAAATAGGGCCAGGGAACAAGGATGGAGAAATGTGCTAAGTGCTGGTGGAAGTGGTGAA GTAAAGGCTCCTTTTCTGGGCAGTGGAGGGACTATAGCTCCATCATCTTTTTCTTCTCGA GGACAGTATGAACATTACCATGCCATTTTTGACCAAATGCAGCAACAAAGAGCAGAAGAT AATGAAGCTAAATGGAAAAGAGAAATATATGGTCGAGGTCTTCCAGAAAGAGGAATTCTG CCTGGAGTTCGTCCAGGATTTCCTTATGGGGCTGCAGGTCATCACCATTTTCCTGATGCT GATGATATTAGAAAAACTTTGAAAAGATTGAAGGCGGTGTCTAAACAAGCCAATGCAAAC AGGCAAAAAGGGCAGCTAGCTGTAGAAAGAGCTAAACAAGTAGAAGAGTTCCTGCAGCGA AAACGGGAAGCTATGCAGAATAAAGCTCGAGCCGAAGGACATATGGTTTATCTGGCAAGA CTGAGGCAAATAAGACTACAGAATTTCAATGAGCGCCAACAGATTAAAGCCAAACTTCGT GGTGAAAAGAAAGAAGCTAATCATTCTGAAGGACAAGAAGGAAGTGAAGAGGCTGACATG AGGCGCAAAAAAATCGAATCACTGAAGGCCCATGCAAATGCACGTGCTGCTGTACTAAAA GAACAACTAGAACGAAAGAGAAAGGAGGCTTATGAGAGAGAAAAAAAAGTGTGGGAAGAG CATTTGGTGGCTAAAGGAGTTAAGAGTTCTGATGTTTCTCCACCTTTGGGACAGCATGAA ACAGGTGGCTCTCCATCAAAGCAACAGATGAGATCTGTTATTTCTGTAACTTCAGCTTTG AAAGAAGTTGGCGTGGACAGTAGTTTAACTGATACCCGGGAAACTTCAGAAGAGATGCAA AAGACCAACAATGCTATTTCAAGTAAGCGAGAAATACTTCGTAGATTAAATGAAAATCTT AAAGCTCAAGAAGATGAAAAAGGAAAGCAGAATCTCTCTGATACTTTTGAGATAAATGTT CATGAAGATGCCAAAGAGCATGAAAAAGAAAAATCAGTTTCATCTGATCGCAAGAAGTGG GAGGCAGGAGGTCAACTTGTGATTCCTCTGGATGAGTTAACACTAGATACATCCTTCTCT ACAACTGAAAGACATACAGTGGGAGAAGTTATTAAATTAGGTCCTAATGGATCTCCAAGA AGAGCCTGGGGGAAAAGTCCGACAGATTCTGTTCTAAAGATACTTGGAGAAGCTGAACTA CAACTTCAGACAGAACTATTAGAAAATACAACTATTAGAAGTGAGATTTCTCCCGAAGGG GAAAAGTACAAACCCTTAATTACTGGAGAAAAAAAAGTACAATGTATTTCACATGAAATA AACCCATCAGCTATTGTTGATTCTCCTGTTGAGACAAAAAGTCCCGAGTTCAGTGAGGCA TCTCCACAGATGTCATTGAAACTGGAAGGAAATTTAGAAGAACCTGATGATTTGGAAACA GAAATTCTACAAGAGCCAAGTGGAACAAACAAAGATGAGAGCTTGCCATGCACTATTACT GATGTGTGGATTAGTGAGGAAAAAGAAACAAAGGAAACTCAGTCGGCAGATAGGATCACC ATTCAGGAAAATGAAGTTTCTGAAGATGGAGTCTCGAGTACTGTGGACCAACTTAGTGAC ATTCATATAGAGCCTGGAACCAATGATTCTCAGCACTCTAAATGTGATGTAGATAAGTCT GTGCAACCGGAACCATTTTTCCATAAGGTGGTTCATTCTGAACACTTGAACTTAGTCCCT CAAGTTCAATCAGTTCAGTGTTCACCAGAAGAATCCTTTGCATTTCGATCTCACTCGCAT TTACCACCAAAAAATAAAAACAAGAATTCCTTGCTGATTGGACTTTCAACTGGTCTGTTT GATGCAAACAACCCAAAGATGTTAAGGACATGTTCACTTCCAGATCTCTCAAAGCTGTTC AGAACCCTTATGGATGTTCCCACCGTAGGAGATGTTCGTCAAGACAATCTTGAAATAGAT GAAATTGAAGATGAAAACATTAAAGAAGGACCTTCTGATTCTGAAGACATTGTGTTTGAA GAAACTGACACAGATTTACAAGAGCTGCAGGCCTCGATGGAACAGTTACTTAGGGAACAA CCTGGTGAAGAATACAGTGAAGAAGAAGAGTCAGTCTTGAAGAACAGTGATGTGGAGCCA ACTGCAAATGGGACAGATGTGGCAGATGAAGATGACAATCCCAGCAGTGAAAGTGCCCTG AACGAAGAATGGCACTCAGATAACAGTGATGGTGAAATTGCTAGTGAATGTGAATGCGAT AGTGTCTTTAACCATTTAGAGGAACTGAGACTTCATCTGGAGCAGGAAATGGGCTTTGAA AAATTCTTTGAGGTTTATGAGAAAATAAAGGCTATTCATGAAGATGAAGATGAAAATATT GAAATTTGTTCAAAAATAGTTCAAAATATTTTGGGAAATGAACATCAGCATCTTTATGCC AAGATTCTTCATTTAGTCATGGCAGATGGAGCCTACCAAGAAGATAATGATGAATAA Clone variation with respect to NM_012224.2 98 a=>t;178 c=>a >OriGene 5' read for mutant NM_012224 unedited
CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAGAACTAGTCT CGAACGCCGCCCGTCCCCACCCCGCCGTGGCCACTAGCAACGACCTCTGTGAAGTTGGAGAGGCGGTAAC GGAGGCACTCCCCCTGCTGCACCCCGCCGTTTCTACGGGGCTCAGAAAACCAGTTTGTGTTGTTTCGTCG GGGTAGTGTCGACCCTGTCTTTACGGGCGTCGCCCCGAGACCAGGACGGGAGTCAAACCCGTGGTATCAA CTTGAGGACGAGTGTCAGGATGTCATTTTTCAAAATGGCGGGGATGGTCCCTCTGCTTTATTAAGCCCCG GTGGGAAGAACTGGCCCACCTAGCCTGGTGGCTTATTGGTCATCACCCGTTTTTCCTACTACCGTCTGGG GTAATGGAGTTTTTAAATGGCCTGGCTTACCTGTAAGGTTGCCTTGGAAAAGAATCTATGAGAGATTTGT TAGACACTACGGGAAATTGGAGAGGTTCATTGGGAAAACCCATTTTGTGTTAACTCACGAGAGAGGGCAC AGTTTTGTTATCTGGAATTACCCTCTCAGAGAGTGCCCGTAGGAGAGAGAGATCCAGGAAGAGTTTGCGT ATTGGCAACTGTGGAATCACAAAATGTCCGATGGGATCATTGAGAAATGCTCTCTCACAGTATGGGATCT GGAGAGGAGTGTGTACAATATGCTCGAAGCCTGTCAAGATCAATTGCCGTGTACAATGTGCGACGTCTCG AACTCTCGCATATCGACACTCTCATGAGACCACTGGGAAGTGGATGTGCGTTCTCTATATCGGG >SC323341 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAGAACTAGTCTCGAAC GCCGCCCGTCCCCACCCCGCCGTGGCCACTAGCAACGACCTCTGTGAAGTTGGAGAGGCGGTAACGGAGG CACTCCCCCTGCTGCACCCCGCCGTTTCTACGGGGCTCAGAAACC |
Restriction Sites | Please inquire |
ACCN | NM_012224 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_012224.1, NP_036356.1 |
RefSeq Size | 5501 bp |
RefSeq ORF | 3777 bp |
Locus ID | 4750 |
UniProt ID | Q96PY6 |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221729 | NEK1 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2 |
CNY 8,760.00 |
|
RC221729L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, Myc-DDK-tagged |
CNY 11,160.00 |
|
RC221729L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, mGFP tagged |
CNY 11,500.00 |
|
RC221729L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, Myc-DDK-tagged |
CNY 11,500.00 |
|
RC221729L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, mGFP tagged |
CNY 11,500.00 |
|
RG221729 | NEK1 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2 |
CNY 10,360.00 |
|
SC309937 | NEK1 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2 |
CNY 24,230.00 |