USP15 (NM_006313) Human Untagged Clone
CAT#: SC314981
USP15 (untagged)-Human ubiquitin specific peptidase 15 (USP15)
CNY 10,464.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | UNPH-2; UNPH4 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_006313 edited
ATGGCGGAAGGCGGAGCGGCGGATCTGGACACCCAGCGGTCTGACATCGCGACGCTGCTC AAAACCTCGCTCCGGAAAGGGGACACCTGGTACCTAGTCGATAGTCGCTGGTTCAAACAG TGGAAAAAATATGTTGGCTTTGACAGTTGGGACAAATACCAGATGGGAGATCAAAATGTG TATCCTGGACCCATTGATAACTCTGGACTTCTCAAAGATGGTGATGCCCAGTCACTTAAG GAACACCTTATTGATGAATTGGATTACATACTGTTGCCAACTGAAGGTTGGAATAAACTT GTCAGCTGGTACACATTGATGGAAGGTCAAGAGCCAATAGCACGAAAGGTGGTTGAACAG GGTATGTTTGTAAAGCACTGCAAAGTAGAAGTATATCTCACAGAATTGAAGCTATGTGAA AATGGAAACATGAATAATGTTGTAACTCGAAGATTTAGCAAAGCTGACACAATAGATACA ATTGAAAAGGAAATAAGAAAAATCTTCAGTATTCCAGATGAAAAGGAGACCAGATTGTGG AACAAATACATGAGTAACACATTTGAACCACTGAATAAACCAGACAGCACCATTCAGGAT GCTGGTTTATACCAAGGACAGGTATTAGTGATAGAACAGAAAAATGAAGATGGAACATGG CCAAGGGGTCCTTCTACTCCTAATGTGAAAAACTCAAATTACTGTCTTCCATCATATACC GCTTATAAGAACTATGATTATTCGGAACCTGGAAGAAACAATGAACAGCCAGGCCTCTGT GGCCTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCTATTCAGTGTTTGAGCAAC ACACCTCCACTTACTGAGTATTTCCTCAATGATAAGTATCAAGAAGAACTGAATTTTGAC AATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCTTATGCCGAACTGATCAAGCAAATG TGGTCTGGAAAGTTTAGCTACGTCACCCCAAGAGCCTTTAAGACACAGGTAGGACGTTTT GCACCTCAGTTCTCTGGATATCAGCAGCAAGACTGTCAAGAACTGTTAGCTTTCCTATTA GATGGATTACATGAGGATTTGAATAGAATTAGGAAAAAACCATATATACAATTAAAAGAT GCAGATGGAAGGCCAGATAAGGTGGTTGCCGAAGAAGCCTGGGAAAACCATTTAAAACGA AATGATTCTATCATAGTAGATATATTTCATGGCCTTTTCAAATCAACTTTAGTTTGTCCT GAGTGTGCTAAGATTTCAGTAACATTTGATCCTTTTTGTTACTTGACACTTCCATTGCCC ATGAAAAAAGAACGCACCTTGGAAGTTTACTTAGTTAGAATGGATCCACTTACCAAACCT ATGCAGTACAAAGTGGTTGTCCCCAAAATTGGAAACATATTAGATCTTTGTACAGCATTG TCTGCTTTGTCAGGAATACCTGCAGATAAGATGATAGTTACTGATATATACAATCATAGA TTTCACAGAATATTCGCTATGGATGAAAACCTTAGTAGTATTATGGAACGGGATGATATT TATGTGTTTGAAATTAACATCAATAGGACAGAAGATACAGAGCACGTGATTATTCCTGTT TGCCTAAGAGAAAAATTCAGACACTCGAGTTATACCCACCATACTGGTTCTTCACTTTTT GGTCAGCCCTTTCTTATGGCTGTACCACGAAACAATACTGAAGACAAACTTTATAATCTC CTGCTCTTGAGAATGTGCCGATATGTCAAAATATCTACTGAAACTGAAGAAACTGAAGGA TCCCTACACTGCTGTAAGGACCAAAATATTAATGGGAATGGCCCAAATGGCATACATGAA GAAGGCTCACCAAGTGAAATGGAAACAGATGAGCCAGATGATGAATCCAGCCAGGATCAA GAACTTCCCTCAGAGAATGAAAACAGTCAGTCTGAAGATTCAGTTGGAGGAGATAATGAT TCTGAAAATGGATTATGTACTGAGGATACTTGCAAAGGTCAACTCACGGGACACAAAAAA CGATTGTTTACATTCCAGTTCAACAACTTAGGCAATACTGATATCAACTACATCAAAGAT GATACCAGGCATATAAGATTTGATGATAGGCAGCTTAGGCTAGATGAAAGATTTTTTCTT GCTTTGGATTGGGATCCTGATTTGAAAAAAAGATATTTTGATGAAAATGCTGCCGAGGAC TTTGAAAAACATGAAAGTGTGGAGTATAAACCTCCTAAAAAACCCTTTGTGAAATTAAAA GATTGCATTGAACTTTTTACAACAAAAGAAAAGCTAGGTGCTGAAGATCCCTGGTATTGT CCGAATTGTAAAGAACATCAGCAAGCCACAAAGAAATTGGATTTATGGTCCCTGCCTCCA GTACTTGTAGTACATCTCAAGCGATTTTCTTACAGTCGATACATGAGAGACAAGTTGGAT ACCTTAGTTGATTTTCCTATCAATGACTTGGATATGTCGGAATTCTTAATTAATCCAAAT GCAGGTCCTTGCCGCTATAATCTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGA GGACACTATACTGCTTTTGCAAAAAATAAAGATGATGGAAAATGGTACTATTTTGATGAC AGTAGTGTCTCCACTGCATCTGAAGACCAAATTGTGTCCAAAGCAGCATATGTACTCTTC TACCAGAGACAAGACACTTTCAGTGGAACTGGCTTTTTTCCTCTTGACCGAGAAACTAAA GGTGCTTCAGCTGCCACTGGCATCCCATTAGAAAGTGATGAAGATAGCAATGATAATGAC AATGATATAGAAAATGAAAACTGTATGCACACTAACTAA |
Restriction Sites | Please inquire |
ACCN | NM_006313 |
Insert Size | 3100 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | The ORF of this clone has been fully sequenced and found to contain one SNP compared with the protein associated with reference NM_006313.1. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006313.1, NP_006304.1 |
RefSeq Size | 4611 bp |
RefSeq ORF | 2859 bp |
Locus ID | 9958 |
UniProt ID | Q9Y4E8 |
Domains | UCH, DUSP |
Protein Families | Druggable Genome, Protease |
Gene Summary | This gene encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222642 | USP15 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 15 (USP15) |
CNY 10,456.00 |
|
RC222642L1 | Lenti ORF clone of Human ubiquitin specific peptidase 15 (USP15), Myc-DDK-tagged |
CNY 12,856.00 |
|
RC222642L2 | Lenti ORF clone of Human ubiquitin specific peptidase 15 (USP15), mGFP tagged |
CNY 9,030.00 |
|
RC222642L3 | Lenti ORF clone of Human ubiquitin specific peptidase 15 (USP15), Myc-DDK-tagged |
CNY 12,856.00 |
|
RC222642L4 | Lenti ORF clone of Human ubiquitin specific peptidase 15 (USP15), mGFP tagged |
CNY 12,856.00 |
|
RG222642 | USP15 (tGFP-tagged) - Human ubiquitin specific peptidase 15 (USP15) |
CNY 12,056.00 |