Aconitase 1 (ACO1) (NM_002197) Human Untagged Clone

CAT#: SC322429

ACO1 (untagged)-Human aconitase 1, soluble (ACO1)



  "NM_002197" in other vectors (7)

CNY 6,520.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-ACO1 Antibody
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for SC322429 GCCGTGCAGTCGGAGGAACACGTGGCCATCAGTAATCATGAGCAACCCATTCGCACACCT
TGCTGAGCCATTGGATCCTGTACAACCAGGAAAGAAATTCTTCAATTTGAATAAATTGGA
GGATTCAAGATATGGGCGCTTACCATTTTCGATCAGAGTTCTTCTGGAAGCAGCCATTCG
GAATTGTGATGAGTTTTTGGTGAAGAAACAGGATATTGAAAATATTCTACATTGGAATGT
CACGCAGCACAAGAACATAGAAGTGCCATTTAAGCCTGCTCGTGTCATCCTGCAGGACTT
TACGGGTGTGCCCGCTGTGGTTGACTTTGCTGCAATGCGTGATGCTGTGAAAAAGTTAGG
AGGAGATCCAGAGAAAATAAACCCTGTCTGCCCTGCTGATCTTGTAATAGATCATTCCAT
CCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAGAAGAATCAAGACCTGGAATTTGA
AAGAAATAGAGAGCGATTTGAATTTTTAAAGTGGGGTTCCCAGGCTTTTCACAACATGCG
GATTATTCCCCCTGGCTCAGGAATCATCCACCAGGTGAATTTGGAATATTTGGCAAGAGT
GGTATTTGATCAGGATGGATATTATTACCCAGACAGCCTCGTGGGCACAGACTCGCACAC
TACCATGATTGATGGCTTGGGCATTCTTGGTTGGGGTGTCGGTGGTATTGAAGCAGAAGC
TGTCATGCTGGGTCAGCCAATCAGTATGGTGCTTCCTCAGGTGATTGGCTACAGGCTGAT
GGGGAAGCCCCACCCTCTGGTAACATCCACTGACATCGTGCTCACCATTACCAAGCACCT
CCGCCAGGTTGGGGTAGTGGGCAAATTTGTCGAGTTCTTCGGGCCTGGAGTAGCCCAGTT
GTCCATTGCTGACCGAGCTACGATTGCTAACATGTGTCCAGAGTACGGAGCAACTGCTGC
CTTTTTCCCAGTTGATGAAGTTAGTATCACGTACCTGGTGCAAACAGGTCGTGATGAAGA
AAAATTAAAGTATATTAAAAAATATCTTCAGGCTGTAGGAATGTTTCGAGATTTCAATGA
CCCTTCTCAAGACCCAGACTTCACCCAGGTTGTGGAATTAGATTTGAAAACAGTAGTGCC
TTGCTGTAGTGGACCCAAAAGGCCTCAGGACAAAGTTGCTGTGTCCGACATGAAAAAGGA
CTTTGAGAGCTGCCTTGGAGCCAAGCAAGGATTTAAAGGATTCCAAGTTGCTCCTGAACA
TCATAATGACCATAAGACCTTTATCTATGATAACACTGAATTCACCCTTGCTCATGGTTC
TGTGGTCATTGCTGCCATTACTAGCTGCACAAACACCAGTAATCCGTCTGTGATGTTAGG
GGCAGGATTGTTAGCAAAGAAAGCTGTGGATGCTGGCCTGAACGTGATGCCTTACATCAA
AACTAGCCTGTCTCCTGGGAGTGGCGTGGTCACCTACTACCTACAAGAAAGCGGAGTCAT
GCCTTATCTGTCTCAGCTTGGGTTTGACGTGGTGGGCTATGGCTGCATGACCTGCATTGG
CAACAGTGGGCCTTTACCTGAACCTGTGGTAGAAGCCATCACACAGGGAGACCTTGTAGC
TGTTGGAGTACTATCTGGAAACAGGAATTTTGAAGGTCGAGTTCACCCCAACACCCGGGC
CAACTATTTAGCCTCTCCCCCCTTAGTAATAGCATATGCAATTGCTGGAACCATCAGAAT
CGACTTTGAGAAAGAGCCATTGGGAGTAAATGCAAAGGGACAGCAGGTATTTCTGAAAGA
TATCTGGCCGACTAGAGACGAGATCCAGGCAGTGGAGCGTCAGTATGTCATCCCGGGGAT
GTTTAAGGAAGTCTATCAGAAAATAGAGACTGTGAATGAAAGCTGGAATGCCTTAGCAAC
CCCATCAGATAAGCTGTTTTTCTGGAATTCCAAATCTACGTATATCAAATCACCACCATT
CTTTGAAAACCTGACTTTGGATCTTCAGCCCCCTAAATCTATAGTGGATGCCTATGTGCT
GCTAAATTTGGGAGATTCGGTAACAACTGACCACATCTCCCCAGCTGGAAATATTGCAAG
AAACAGTCCTGCTGCTCGCTACTTAACTAACAGAGGCCTAACTCCACGAGAATTCAACTC
CTATGGCTCCCGCCGAGGTAATGACGCCGTCATGGCACGGGGAACATTTGCCAACATTCG
CTTGTTAAACAGATTTTTGAACAAGCAGGCACCACAGACTATCCATCTGCCTTCTGGGGA
AATCCTTGATGTGTTTGATGCTGCTGAGCGGTACCAGCAGGCAGGCCTTCCCCTGATCGT
TCTGGCTGGCAAAGAGTACGGTGCAGGCAGCTCCCGAGACTGGGCAGCTAAGGGCCCTTT
CCTGCTGGGAATCAAAGCCGTCCTGGCCGAGAGCTACGAGCGCATTCACCGCAGTAACCT
GGTTGGGATGGGTGTGATCCCACTTGAATATCTCCCTGGTGAGAATGCAGATGCCCTGGG
GCTCACAGGGCAAGAACGATACACTATCATTATTCCAGAAAACCTCAAACCACAAATGAA
AGTCCAGGTCAAGCTGGATACTGGCAAGACCTTCCAGGCTGTCATGAGGTTTGACACTGA
TGTGGAGCTCACTTATTTCCTCAACGGGGGCATCCTCAACTACATGATCCGCAAGATGGC
CAAGTAGGAGACGTGCACTTGGTGCTGCGCCCAGGGAGGAAGCCGCACCACCAGCCAGCG
CAGGCCCTGGTGGAGAGGCCTCCCTGGCTGCCTCTGGGAGGGGTGCTGCCTTGTAGATGG
AGCAAGTGAGCACTGAGGGTCTGGTGCCAATCCTGTAGGCACAAAACCAGAAGTTTCTAC
ATTCTCTATTTTTGTTAATCATCTTCTCTTTTTCCAGAATTTGGAAGCTAGAATGGTGGG
AATGTCAGTAGTGCCAGAAAGAGAGAACCAAGCTTGTCTTTAAAGTTACTGATCACAGGA
CGTTGCTTTTTCACTGTTTCCTATTAATCTTCAGCTGAACACAAGCAAACCTTCTCAGGA
GGTGTCTCCTACCCTCTTATTGTTCCTCTTACGCTCTGCTCAATGAAACCTTCCTCTTGA
GGGTCATTTTCCTTTCTGTATTAATTATACCAGTGTTAAGTGACATAGATAAGAACTTTG
CACACTTCAAATCAGAGCAGTGATTCTCTCTTCTCTCCCCTTTTCCTTCAGAGTGAATCA
TCCAGACTCCTCATGGATAGGTCGGGTGTTAAAGTTGTTTTGATTATGTACCTTTTGATA
GATCCACATAAAAAGAAATGTGAAGTTTTCTTTTACTATCTTTTCATTTATCAAGCAGAG
ACCTTTGTTGGGAGGCGGTTTGGGAGAACACATTTCTAATTTGAATGAAATGAAATCTAT
TTTCAGTGAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_002197
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002197.1, NP_002188.1
RefSeq Size 3498 bp
RefSeq ORF 2670 bp
Locus ID 48
UniProt ID P21399
Domains Aconitase_C, aconitase
Protein Families Druggable Genome
Protein Pathways Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways
Gene Summary The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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