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Aconitase 1 (ACO1) (NM_002197) Human Untagged Clone
CAT＃: SC126974
ACO1 (untagged)-Human aconitase 1, soluble (ACO1)
CNY 6,520.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126974 sequence for NM_002197 edited (data generated by NextGen Sequencing)
ATGAGCAACCCATTCGCACACCTTGCTGAGCCATTGGATCCTGTACAACCAGGAAAGAAA TTCTTCAATTTGAATAAATTGGAGGATTCAAGATATGGGCGCTTACCATTTTCGATCAGA GTTCTTCTGGAAGCAGCCATTCGGAATTGTGATGAGTTTTTGGTGAAGAAACAGGATATT GAAAATATTCTACATTGGAATGTCACGCAGCACAAGAACATAGAAGTGCCATTTAAGCCT GCTCGTGTCATCCTGCAGGACTTTACGGGTGTGCCCGCTGTGGTTGACTTTGCTGCAATG CGTGATGCTGTGAAAAAGTTAGGAGGAGATCCAGAGAAAATAAACCCTGTCTGCCCTGCT GATCTTGTAATAGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAG AAGAATCAAGACCTGGAATTTGAAAGAAATAGAGAGCGATTTGAATTTTTAAAGTGGGGT TCCCAGGCTTTTCACAACATGCGGATTATTCCCCCTGGCTCAGGAATCATCCACCAGGTG AATTTGGAATATTTGGCAAGAGTGGTATTTGATCAGGATGGATATTATTACCCAGACAGC CTCGTGGGCACAGACTCGCACACTACCATGATTGATGGCTTGGGCATTCTTGGTTGGGGT GTCGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGCCAATCAGTATGGTGCTTCCT CAGGTGATTGGCTACAGGCTGATGGGGAAGCCCCACCCTCTGGTAACATCCACTGACATC GTGCTCACCATTACCAAGCACCTCCGCCAGGTTGGGGTAGTGGGCAAATTTGTCGAGTTC TTCGGGCCTGGAGTAGCCCAGTTGTCCATTGCTGACCGAGCTACGATTGCTAACATGTGT CCAGAGTACGGAGCAACTGCTGCCTTTTTCCCAGTTGATGAAGTTAGTATCACGTACCTG GTGCAAACAGGTCGTGATGAAGAAAAATTAAAGTATATTAAAAAATATCTTCAGGCTGTA GGAATGTTTCGAGATTTCAATGACCCTTCTCAAGACCCAGACTTCACCCAGGTTGTGGAA TTAGATTTGAAAACAGTAGTGCCTTGCTGTAGTGGACCCAAAAGGCCTCAGGACAAAGTT GCTGTGTCCGACATGAAAAAGGACTTTGAGAGCTGCCTTGGAGCCAAGCAAGGATTTAAA GGATTCCAAGTTGCTCCTGAACATCATAATGACCATAAGACCTTTATCTATGATAACACT GAGTTCACCCTTGCTCATGGTTCTGTGGTCATTGCTGCCATTACTAGCTGCACAAACACC AGTAATCCGTCTGTGATGTTAGGGGCAGGATTGTTAGCAAAGAAAGCTGTGGATGCTGGC CTGAACGTGATGCCTTACATCAAAACTAGCCTGTCTCCTGGGAGTGGCGTGGTCACCTAC TACCTACAAGAAAGCGGAGTCATGCCTTATCTGTCTCAGCTTGGGTTTGACGTGGTGGGC TATGGCTGCATGACCTGCATTGGCAACAGTGGGCCTTTACCTGAACCTGTGGTAGAAGCC ATCACACAGGGAGACCTTGTAGCTGTTGGAGTACTATCTGGAAACAGGAATTTTGAAGGT CGAGTTCACCCCAACACCCGGGCCAACTATTTAGCCTCTCCCCCCTTAGTAATAGCATAT GCAATTGCTGGAACCATCAGAATCGACTTTGAGAAAGAGCCATTGGGAGTAAATGCAAAG GGACAGCAGGTATTTCTGAAAGATATCTGGCCGACTAGAGACGAGATCCAGGCAGTGGAG CGTCAGTATGTCATCCCGGGGATGTTTAAGGAAGTCTATCAGAAAATAGAGACTGTGAAT GAAAGCTGGAATGCCTTAGCAACCCCATCAGATAAGCTGTTTTTCTGGAATTCCAAATCT ACGTATATCAAATCACCACCATTCTTTGAAAACCTGACTTTGGATCTTCAGCCCCCTAAA TCTATAGTGGATGCCTATGTGCTGCTAAATTTGGGAGATTCGGTAACAACTGACCACATC TCCCCAGCTGGAAATATTGCAAGAAACAGTCCTGCTGCTCGCTACTTAACTAACAGAGGC CTAACTCCACGAGAATTCAACTCCTATGGCTCCCGCCGAGGTAATGACGCCGTCATGGCA CGGGGAACATTTGCCAACATTCGCTTGTTAAACAGATTTTTGAACAAGCAGGCACCACAG ACTATCCATCTGCCTTCTGGGGAAATCCTTGATGTGTTTGATGCTGCTGAGCGGTACCAG CAGGCAGGCCTTCCCCTGATCGTTCTGGCTGGCAAAGAGTACGGTGCAGGCAGCTCCCGA GACTGGGCAGCTAAGGGCCCTTTCCTGCTGGGAATCAAAGCCGTCCTGGCCGAGAGCTAC GAGCGCATTCACCGCAGTAACCTGGTTGGGATGGGTGTGATCCCACTTGAATATCTCCCT GGTGAGAATGCAGATGCCCTGGGGCTCACAGGGCAAGAACGATACACTATCATTATTCCA GAAAACCTCAAACCACAAATGAAAGTCCAGGTCAAGCTGGATACTGGCAAGACCTTCCAG GCTGTCATGAGGTTTGACACTGATGTGGAGCTCACTTATTTCCTCAACGGGGGCATCCTC AACTACATGATCCGCAAGATGGCCAAGTAG Clone variation with respect to NM_002197.2 1263 a=>g >OriGene 5' read for NM_002197 unedited
GGCCGCGAATTCGGCACCAGCGCACGGGAACGCGTCCCGCTGCTTGGGTCAGGTTCGCCG GTCGCGGGAGCCCCGCCGTGCAGTCGGAGGAACACGTGGCCATCAGTAATCATGAGCAAC CCATTCGCACACCTTGCTGAGCCATTGGATCCTGTACAACCAGGAAAGAAATTCTTCAAT TTGAATAAATTGGAGGATTCAAGATATGGGCGCTTACCATTTTCGATCAGAGTTCTTCTG GAAGCAGCCATTCGGAATTGTGATGAGTTTTTGGTGAAGAAACAGGATATTGAAAATATT CTACATTGGAATGTCACGCAGCACAAGAACATAGAAGTGCCATTTAAGCCTGCTCGTGTC ATCCTGCAGGACTTTACGGGTGTGCCCGCTGTGGTTGACTTTGCTGCAATGCGTGATGCT GTGAAAAAGTTAGGAGGAGATCCAGAGAAAATAAACCCTGTCTGCCCTGCTGATCTTGTA ATAGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAGAAGAATCAA GACCTGGAATTTGAAAGAAATAGAGAGCGATTTGAATTTTTAAAGTGGGGTTCCCAGGCT TTTCACAACATGCGGATTATTCCCCCTGGCTCAGGAATCATCCACCAGGTGAATTTGGAA TATTTGGNCAGAGTGGTATTTGATCAGGATGGATATTATTACCCAGACAGCCTCGTGGGC ACAGACTCGCACACTACCT >OriGene 3' read for NM_002197 unedited
CGCATTTGTCTGAANCGCGGCCCGCAAACTACCAGTCGAGCTTTTTTTTTTTTTTTTTTT TTCATGAAAAAGATTCATTTCATTCAAATCAGAAATGTGTTCTCCCAAACCGCCTCCCAC AAAGGTCTCTGCTTGATCAATGAAAAGATAGTAAAAGAAACCTTCACATTTCTTTTTATG TGGATCTATTAAAGGGTACATAATCAAAACAACTTTAACACCCGACCTATCCATGAGGAG TCTGGATGATTCACTCTGACGGAAAAGGGGAGAGAAGAGAGAATCACTGCTCTGATTCGA AGCGTGCAACGTTCTTATCTATGCCACTCACCACTGGTATACTTAATACTGACGGGAAAC TGACCTTCAAAAGGAAGGCTTCATTGACCAGACCGTAACAGGAACAATACGAGGGCAGGA GACACCTCCTGAGAAGGATTGCTTGCGCTCAGCTGACCATTACTCGGAACCCACTAACAC CCCATCGTCCTGCGACCACTCACTTTAACAGACCCCTTGGCTTCTATCTTTCTGCCAATA CTGCCATTCCCCCCCTTCTACCCTCCACATTCTGCACACCGACATGACGCCTACCACCCC TTCCGACTGCACTACCCTCCTGCCTCCGTTCTCCACGGATTGGCACCAAACCCTCTGGGC TCACTTCTTTTTTTTACCGGACCAACCCCTACCACAAGAACTCGCTGAGGACTTTTCAAC CGGGATTTGTAATACCTGTAGCATCCGCCTACCTCCTTTGCACCCCACCATCACAACTCA CCATCCCCCTACACACCCCATCTATCTACCACACACTGCCACAATTAACCTTTCCCCACG TTCCACCTAAATATCCGCCCATCCCACATACACATATCCCCTCCTCTACTTCCATCCCTA CTCTCACGCGCTCTATCACAACTCACAACCACATGCTCATCGGCCCACTTTACCTCAACN ACCACCTACCGCCTCTTACCTACAATAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002197 |
| Insert Size | 3460 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002197.1, NP_002188.1 |
| RefSeq Size | 3498 bp |
| RefSeq ORF | 2670 bp |
| Locus ID | 48 |
| UniProt ID | P21399 |
| Domains | Aconitase_C, aconitase |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways |
| Gene Summary | The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. |
Citations (2)
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201857 | ACO1 (Myc-DDK-tagged)-Human aconitase 1, soluble (ACO1) |
CNY 6,512.00 |
|
| RC201857L1 | Lenti ORF clone of Human aconitase 1, soluble (ACO1), Myc-DDK-tagged |
CNY 8,912.00 |
|
| RC201857L2 | Lenti ORF clone of Human aconitase 1, soluble (ACO1), mGFP tagged |
CNY 9,030.00 |
|
| RC201857L3 | Lenti ORF clone of Human aconitase 1, soluble (ACO1), Myc-DDK-tagged |
CNY 8,912.00 |
|
| RC201857L4 | Lenti ORF clone of Human aconitase 1, soluble (ACO1), mGFP tagged |
CNY 8,912.00 |
|
| RG201857 | ACO1 (tGFP-tagged) - Human aconitase 1, soluble (ACO1) |
CNY 8,112.00 |
|
| SC322429 | ACO1 (untagged)-Human aconitase 1, soluble (ACO1) |
CNY 6,520.00 |
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