United States
Germany
Japan
United Kingdom
China
NEK7 (NM_133494) Human Untagged Clone
CAT＃: SC323481
NEK7 (untagged)-Kinase deficient mutant (K63M) of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7)
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323481 sequence for NM_133494 edited (data generated by NextGen Sequencing)
ATGGATGAGCAATCACAAGGAATGCAAGGGCCACCTGTTCCTCAGTTCCAACCACAGAAG GCCTTACGACCGGATATGGGCTATAATACATTAGCCAACTTTCGAATAGAAAAGAAAATT GGTCGCGGACAATTTAGTGAAGTTTATAGAGCAGCCTGTCTCTTGGATGGAGTACCAGTA GCTTTAATGAAAGTGCAGATATTTGATTTAATGGATGCCAAAGCACGTGCTGATTGCATC AAAGAAATAGATCTTCTTAAGCAACTCAACCATCCAAATGTAATAAAATATTATGCATCA TTCATTGAAGATAATGAACTAAACATAGTTTTGGAACTAGCAGATGCTGGCGACCTATCC AGAATGATCAAGCATTTTAAGAAGCAAAAGAGGCTAATTCCTGAAAGAACTGTTTGGAAG TATTTTGTTCAGCTTTGCAGTGCATTGGAACACATGCATTCTCGAAGAGTCATGCATAGA GATATAAAACCAGCTAATGTGTTCATTACAGCCACTGGGGTGGTAAAACTTGGAGATCTT GGGCTTGGCCGGTTTTTCAGCTCAAAAACCACAGCTGCACATTCTTTAGTTGGTACGCCT TATTACATGTCTCCAGAGAGAATACATGAAAATGGATACAACTTCAAATCTGACATCTGG TCTCTTGGCTGTCTACTATATGAGATGGCTGCATTACAAAGTCCTTTCTATGGTGACAAA ATGAATTTATACTCACTGTGTAAGAAGATAGAACAGTGTGACTACCCACCTCTTCCTTCA GATCACTATTCAGAAGAACTCCGACAGTTAGTTAATATGTGCATCAACCCAGATCCAGAG AAGCGACCAGACGTCACCTATGTTTATGACGTAGCAAAGAGGATGCATGCATGCACTGCA AGCAGCTAA Clone variation with respect to NM_133494.2 188 a=>t;189 a=>g >OriGene 5' read for mutant NM_133494 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAACTCCCAACTTCC TGAGTTCTAAAGTTCCTGTTGCTTCAGACAATGGATGAGCAATCACAAGGAATGCAAGGGCCACCTGTTC CTCAGTTCCAACCACAGAAGGCCTTACGACCGGATATGGGCTATAATACATTAGCCAACTTTCGAATAGA AAAGAAAATTGGTCGCGGACAATTTAGTGAAGTTTATAGAGCAGCCTGTCTCTTGGATGGAGTACCAGTA GCTTTAATGAAAGTGCAGATATTTGATTTATGGGATGCAAAGCACGTGCTGATTGCATCAAAAGAAATAG ATTCTTCTTAAGCACTCACCCATCCAAATGTATTAAAAAATAATGCATCATTTCATTGAAGAATAATGAC TAAACCTAGTTTTGAACCTAGCCGAATGCCTGGCCGACTATCAGAAGGATTCAAGCCATTTTAAGAAGCA AAGAAGGCAATTTCTGAAAGAACTGTTGGAAGTATTTGTTAAGCTTGCATGCATTGGAAACAATGCTTTC GAAAGCTAGCTAGAATAACCCAGTTAGGGGTCTTACGCACGGGGGGTAAATTGAATTGGCTGCCGGTTTA CTCAAACCACGTCACTCTTATGGACCTATACATGTCCGAGAGAACTGAGATCCTCACTGAACTGGCCTGC GTCATAGAGGCGCTACAGCTCTTGGCAATGATATCCTGTGAAGAACGTAAACACTCCTGTACATCGAATC GCG >SC323481 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYAAKMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAACTCCCAACTTCCTGAGTT CTAAAGTTCCTGTTGCTTCAGACAATGGATGAGCAATCACAAGGAATGCAAGGGCCACCTGTTCCTCAGT TCCAACCACAGAAGGCCTTACGACCGGATATGGGCTATAATACAT |
| Restriction Sites | Please inquire |
| ACCN | NM_133494 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133494.1, NP_598001.1 |
| RefSeq Size | 1072 bp |
| RefSeq ORF | 909 bp |
| Locus ID | 140609 |
| UniProt ID | Q8TDX7 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211857 | NEK7 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7) |
CNY 2,400.00 |
|
| RC211857L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC211857L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7), mGFP tagged |
CNY 5,890.00 |
|
| RC211857L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211857L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7), mGFP tagged |
CNY 4,800.00 |
|
| RG211857 | NEK7 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7) |
CNY 4,000.00 |
|
| SC120526 | NEK7 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7) |
CNY 2,400.00 |
