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CKII alpha (CSNK2A1) (NM_177559) Human Untagged Clone
CAT＃: SC323516
CSNK2A1 (untagged)-Kinase deficient mutant (K68M) of Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CK2A1; Cka1; Cka2; CKII; OCNDS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_177559, the custom clone sequence may differ by one or more nucleotides
ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCTCGAGAATACT GGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAGCTGGTTCGAAAATTAGGCCG AGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAAAAAGTTGTTGTTAAAATTCTC AAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAGAGGAGGTCCCAACA TCATCACACTGGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTTTTTGAACACGTAAA CAACACAGACTTCAAGCAATTGTACCAGACGTTAACAGACTATGATATTCGATTTTACATGTATGAGATT CTGAAGGCCCTGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCCATAATGTCATGA TTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAGTTTTATCATCCTGGCCAAGA ATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCTGAGCTACTTGTAGACTATCAGATGTACGAT TATAGTTTGGATATGTGGAGTTTGGGTTGTATGCTGGCAAGTATGATCTTTCGGAAGGAGCCATTTTTCC ATGGACATGACAATTATGATCAGTTGGTGAGGATAGCCAAGGTTCTGGGGACAGAAGATTTATATGACTA TATTGACAAATACAACATTGAATTAGATCCACGTTTCAATGATATCTTGGGCAGACACTCTCGAAAGCGA TGGGAACGCTTTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATTTCCTGGACAAAC TGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAGCACCCCTATTTCTACACTGT TGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCAGGGGGCAGTACGCCCGTCAGCAGCGCCAAT ATGATGTCAGGGATTTCTTCAGTGCCAACCCCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTG CTGCTGCCAACCCCCTTGGGATGCCTGTTCCAGCTGCCGCTGGCGCTCAGCAGTAA >OriGene 5' read for mutant NM_177559 unedited
ACGCCCGTAGAGCAATGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTCCTCCTCCA TCGCCGCCATATTGTCTGTGTGAGCAGAGGGGAGAGCGGCCGCCGCCGCTGCCGCTTCCACCACAGCTCT ATCAAGGCTTGTCAAGCAGTGTGCTCATCACATGGTAAATCATGCAGCGTGGAACCTCATAAAATCTCCA AGAAACATCATTCACCCATACTGACTAGTTTCACATCTCTTTGTTTGAAGAAAAACAGGTCTGAAAACAA GGTCTAACCCCCAGCTGCTTCTGAACACAGTGACTGCCAGAATCTCCAAACATCAAGTCCCAGCTTTGTC CGCCAACCGGTCTGACTGGTCGGAACCCGTGCAAGCAGGGCCAGAGTTTACCCAGTGTTAATCCCCCACC CTCGAAAACCTGGAATACAGTTCCAGGTGTGGATTGGGAAATCAAAGGATACACTGGTTCAAATTAGCAG AGTACTCGTGGATTTTTGACCCCCCAAACACATAAAAA >SC323516 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TGATCGAATTATGCCGAGKAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAAAAAGTTG TTGTTATGATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAG AGGAGGTCCCAACATCATCACACTGGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTT TTTGAACACGTAAACAACACAGACTTCAAGCAATTGTACCAGACG |
| Restriction Sites | Please inquire |
| ACCN | NM_177559 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177559.2, NP_808227.1 |
| RefSeq Size | 2849 bp |
| RefSeq ORF | 1176 bp |
| Locus ID | 1457 |
| UniProt ID | P68400 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
| Protein Pathways | Adherens junction, Tight junction, Wnt signaling pathway |
| Gene Summary | Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Apr 2018] Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, 4 and 5 encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222256 | CSNK2A1 (Myc-DDK-tagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1 |
CNY 5,488.00 |
|
| RC222256L3 | Lenti-ORF clone of CSNK2A1 (Myc-DDK-tagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1 |
CNY 7,888.00 |
|
| RC222256L4 | Lenti-ORF clone of CSNK2A1 (mGFP-tagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1 |
CNY 5,890.00 |
|
| RG222256 | CSNK2A1 (tGFP-tagged) - Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1 |
CNY 4,370.00 |
|
| SC107027 | CSNK2A1 (untagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1 |
CNY 5,488.00 |
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