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TIE2 (TEK) (NM_000459) Human Untagged Clone
CAT＃: SC323531
TEK (untagged)-Kinase deficient mutant (K855M) of Human TEK tyrosine kinase, endothelial (TEK)
CNY 7,832.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD202B; GLC3E; TIE-2; TIE2; VMCM; VMCM1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323531 sequence for NM_000459 edited (data generated by NextGen Sequencing)
ATGGACTCTTTAGCCAGCTTAGTTCTCTGTGGAGTCAGCTTGCTCCTTTCTGGAACTGTG GAAGGTGCCATGGACTTGATCTTGATCAATTCCCTACCTCTTGTATCTGATGCTGAAACA TCTCTCACCTGCATTGCCTCTGGGTGGCGCCCCCATGAGCCCATCACCATAGGAAGGGAC TTTGAAGCCTTAATGAACCAGCACCAGGATCCGCTGGAAGTTACTCAAGATGTGACCAGA GAATGGGCTAAAAAAGTTGTTTGGAAGAGAGAAAAGGCTAGTAAGATCAATGGTGCTTAT TTCTGTGAAGGGCGAGTTCGAGGAGAGGCAATCAGGATACGAACCATGAAGATGCGTCAA CAAGCTTCCTTCCTACCAGCTACTTTAACTATGACTGTGGACAAGGGAGATAACGTGAAC ATATCTTTCAAAAAGGTATTGATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGTTCC TTCATCCATTCAGTGCCCCGGCATGAAGTACCTGATATTCTAGAAGTACACCTGCCTCAT GCTCAGCCCCAGGATGCTGGAGTGTACTCGGCCAGGTATATAGGAGGAAACCTCTTCACC TCGGCCTTCACCAGGCTGATAGTCCGGAGATGTGAAGCCCAGAAGTGGGGACCTGAATGC AACCATCTCTGTACTGCTTGTATGAACAATGGTGTCTGCCATGAAGATACTGGAGAATGC ATTTGCCCTCCTGGGTTTATGGGAAGGACGTGTGAGAAGGCTTGTGAACTGCACACGTTT GGCAGAACTTGTAAAGAAAGGTGCAGTGGACAAGAGGGATGCAAGTCTTATGTGTTCTGT CTCCCTGACCCCTATGGGTGTTCCTGTGCCACAGGCTGGAAGGGTCTGCAGTGCAATGAA GCATGCCACCCTGGTTTTTACGGGCCAGATTGTAAGCTTAGGTGCAGCTGCAACAATGGG GAGATGTGTGATCGCTTCCAAGGATGTCTCTGCTCTCCAGGATGGCAGGGGCTCCAGTGT GAGAGAGAAGGCATACCGAGGATGACCCCAAAGATAGTGGATTTGCCAGATCATATAGAA GTAAACAGTGGTAAATTTAATCCCATTTGCAAAGCTTCTGGCTGGCCGCTACCTACTAAT GAAGAAATGACCCTGGTGAAGCCGGATGGGACAGTGCTCCATCCAAAAGACTTTAACCAT ACGGATCATTTCTCAGTAGCCATATTCACCATCCACCGGATCCTCCCCCCTGACTCAGGA GTTTGGGTCTGCAGTGTGAACACAGTGGCTGGGATGGTGGAAAAGCCCTTCAACATTTCT GTTAAAGTTCTTCCAAAGCCCCTGAATGCCCCAAACGTGATTGACACTGGACATAACTTT GCTGTCATCAACATCAGCTCTGAGCCTTACTTTGGGGATGGACCAATCAAATCCAAGAAG CTTCTATACAAACCCGTTAATCACTATGAGGCTTGGCAACATATTCAAGTGACAAATGAG ATTGTTACACTCAACTATTTGGAACCTCGGACAGAATATGAACTCTGTGTGCAACTGGTC CGTCGTGGAGAGGGTGGGGAAGGGCATCCTGGACCTGTGAGACGCTTCACAACAGCTTCT ATCGGACTCCCTCCTCCAAGAGGTCTAAATCTCCTGCCTAAAAGTCAGACCACTCTAAAT TTGACCTGGCAACCAATATTTCCAAGCTCGGAAGATGACTTTTATGTTGAAGTGGAGAGA AGGTCTGTGCAAAAAAGTGATCAGCAGAATATTAAAGTTCCAGGCAACTTGACTTCGGTG CTACTTAACAACTTACATCCCAGGGAGCAGTACGTGGTCCGAGCTAGAGTCAACACCAAG GCCCAGGGGGAATGGAGTGAAGATCTCACTGCTTGGACCCTTAGTGACATTCTTCCTCCT CAACCAGAAAACATCAAGATTTCCAACATTACACACTCCTCGGCTGTGATTTCTTGGACA ATATTGGATGGCTATTCTATTTCTTCTATTACTATCCGTTACAAGGTTCAAGGCAAGAAT GAAGACCAGCACGTTGATGTGAAGATAAAGAATGCCACCATCACTCAGTATCAGCTCAAG GGCCTAGAGCCTGAAACAGCATACCAGGTGGACATTTTTGCAGAGAACAACATAGGGTCA AGCAACCCAGCCTTTTCTCATGAACTGGTGACCCTCCCAGAATCTCAAGCACCAGCGGAC CTCGGAGGGGGGAAGATGCTGCTTATAGCCATCCTTGGCTCTGCTGGAATGACCTGCCTG ACTGTGCTGTTGGCCTTTCTGATCATATTGCAATTGAAGAGAGCAAATGTGCAAAGGAGA ATGGCCCAAGCCTTCCAAAACGTGAGGGAAGAACCAGCTGTGCAGTTCAACTCAGGGACT CTGGCCCTAAACAGGAAGGTCAAAAACAACCCAGATCCTACAATTTATCCAGTGCTTGAC TGGAATGACATCAAATTTCAAGATGTGATTGGGGAGGGCAATTTTGGCCAAGTTCTTAAG GCGCGCATCAAGAAGGATGGGTTACGGATGGATGCTGCCATCATGAGAATGAAAGAATAT GCCTCCAAAGATGATCACAGGGACTTTGCAGGAGAACTGGAAGTTCTTTGTAAACTTGGA CACCATCCAAACATCATCAATCTCTTAGGAGCATGTGAACATCGAGGCTACTTGTACCTG GCCATTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCTTCGCAAGAGCCGTGTGCTG GAGACGGACCCAGCATTTGCCATTGCCAATAGCACCGCGTCCACACTGTCCTCCCAGCAG CTCCTTCACTTCGCTGCCGACGTGGCCCGGGGCATGGACTACTTGAGCCAAAAACAGTTT ATCCACAGGGATCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTATGTGGCAAAAATA GCAGATTTTGGATTGTCCCGAGGTCAAGAGGTGTATGTGAAAAAGACAATGGGAAGGCTC CCAGTGCGCTGGATGGCCATCGAGTCACTGAATTACAGTGTGTACACAACCAACAGTGAT GTATGGTCCTATGGTGTGTTACTATGGGAGATTGTTAGCTTAGGAGGCACACCCTACTGC GGGATGACTTGTGCAGAACTCTACGAGAAGCTGCCCCAGGGCTACAGACTGGAGAAGCCC CTGAACTGTGATGATGAGGTGTATGATCTAATGAGACAATGCTGGCGGGAGAAGCCTTAT GAGAGGCCATCATTTGCCCAGATATTGGTGTCCTTAAACAGAATGTTAGAGGAGCGAAAG ACCTACGTGAATACCACGCTTTATGAGAAGTTTACTTATGCAGGAATTGACTGTTCTGCT GAAGAAGCGGCCTAG Clone variation with respect to NM_000459.3 1962 a=>g;2322 g=>a;2564 a=>t;2565 a=>g >OriGene 5' read for mutant NM_000459 unedited
CCGCCGTTGTCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCCTGCTTCTGT GCTGTTCCTTCTTGCCTCTAACTTGTAAACAAGACGTAGTAGGACGATGCTAATGGAAAGTCACAAACCG CTGGGTTTTTGAAAGGATCCTTGGGACCTCATGCACATTTGTGGAAACTGGATGGAGAGATTTGGGGAAG CATGGACTCTTTAGCCAGCTTAGTTCTCTGTGGAGTCAGCTTTGCTCCTTTCTGGAACTGTGGGAAGGTG CCATGGACTTGATCTTGATCAATTCCCTACCTCTTTGTATCTGATGCTGAAACATCTTCTCACCCTGCAT TGCCCTGGGTGGGCGCCCCCTGAACCCCATCACCCTTAGGGAGGGGACTTGGAGGCCTTATTGAACCAGA CCAGATTCCCGTTGGAAGTTACTAAGGATGTGACCAAAAATGGGCTAAAAATTTGTTGGAAGAGAAAAAA GGCTGAAAAATCATGGGTGCTTATTTCTGAAGGGCGATTCAGGGAAGGGCATTCGGATCCACCCGGGATA TGCTACAAAGTTCTTTCTACGTCTCTTATATTATACTGGACGGGATATACGGCACTTCTCTTCAAGGGTG TGTAAGAAACGCGTATTCAAAGGGTTCTTATCATTGGCCGGTAGATCCGAATTCAGATCCCGTCTAGTCC CACCGAAGTCGAGTAGATCGCGGATATAGGAACCTTTCTGCTCTAACGTATCCGATTGACCAATGGCCCA GCCCATTCTGTGACACGGTTCGAAATGAAGAAATCCGGTGGAACCTGTA >SC323531 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GWGTGATTGGGGAGCATTTTGGCCAGTTCTTAAGGCGCGCATCAAGAAGGATGGGTTACGGATGGATGCT GCCATCATGAGAATGAAAGAATATGCCTCCAAAGATGATCACAGGGACTTTGCAGGAGAACTGGAAGTTC TTTGTAAACTTGGACACCATCCAAACATCATCAATCTCTTAGGAGCATGTGAACATCGAGGCTACTTGTA CCTGGCCATTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCT |
| Restriction Sites | Please inquire |
| ACCN | NM_000459 |
| Insert Size | 4880 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000459.1, NP_000450.1 |
| RefSeq Size | 4787 bp |
| RefSeq ORF | 3375 bp |
| Locus ID | 7010 |
| UniProt ID | Q02763 |
| Domains | pkinase, TyrKc, S_TKc, FN3, EGF, EGF |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane |
| Gene Summary | This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206554 | TEK (Myc-DDK-tagged)-Human TEK tyrosine kinase, endothelial (TEK) |
CNY 11,736.00 |
|
| RC206554L1 | Lenti ORF clone of Human TEK tyrosine kinase, endothelial (TEK), Myc-DDK-tagged |
CNY 14,136.00 |
|
| RC206554L2 | Lenti ORF clone of Human TEK tyrosine kinase, endothelial (TEK), mGFP tagged |
CNY 11,500.00 |
|
| RC206554L3 | Lenti ORF clone of Human TEK tyrosine kinase, endothelial (TEK), Myc-DDK-tagged |
CNY 14,136.00 |
|
| RC206554L4 | Lenti ORF clone of Human TEK tyrosine kinase, endothelial (TEK), mGFP tagged |
CNY 14,136.00 |
|
| RC600057 | TEK (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens TEK tyrosine kinase, endothelial, Signal peptide (1-22) plus EC domain (23-748) |
CNY 5,480.00 |
|
| RG206554 | TEK (tGFP-tagged) - Human TEK tyrosine kinase, endothelial (TEK) |
CNY 13,336.00 |
|
| SC119843 | TEK (untagged)-Human TEK tyrosine kinase, endothelial (TEK) |
CNY 7,832.00 |
