PFTK2 (BC038807) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ALS2CR7; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7; PFTAIRE protein kinase 2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC038807, the custom clone sequence may differ by one or more nucleotides ATGACTTCATTTCACCCCAGGGGACTTCAAGCTGCCCGTGCCCAGAAGTTCAAGAGTAAAAGGCCACGGA GTAACAGTGATTGTTTTCAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGGAGGAAGAGCCTCCCTTTTGG GGCAGCCTCATCTTACTTGAACTTGGAGAAGCTGGGTGAAGGCTCTTATGCGACAGTTTACAAGGGGATT AGCAGAATAAATGGACAACTAGTGGCTTTAAAAGTCATCAGCATGAATGCAGAGGAAGGAGTCCCATTTA CAGCTATCCGAGAAGCTTCTCTCCTGAAGGGTTTGAAACATGCCAATATTGTGCTCCTGCATGACATAAT CCACACCAAAGAGACACTGACATTCGTTTTTGAATACATGCACACAGACCTGGCCCAGTATATGTCTCAG CATCCAGGAGGGCTTCATCCTCATAATGTCAGACTTTTCATGTTTCAACTTTTGCGGGGCCTGGCGTACA TCCACCACCAACACGTTCTTCACAGGGACCTGAAACCTCAGAACTTACTCATCAGTCACCTGGGAGAGCT CAAACTGGCTGATTTTGGTCTTGCCCGGGCCAAGTCCATTCCCAGCCAGACATACTCTTCAGAAGTCGTG ACCCTCTGGTACCGGCCCCCTGATGCTTTGCTGGGAGCCACTGAATATTCCTCTGAGCTGGACATATGGG GTGCAGGCTGCATCTTTATTGAAATGTTCCAGGGTCAACCTTTGTTTCCTGGGGTTTCCAACATCCTTGA ACAGCTGGAGAAAATCTGGGAGGTGCTGGGAGTCCCTACAGAGGATACTTGGCCGGGAGTCTCCAAGCTA CCTAACTACAATCCAGAATGGTTCCCACTGCCTACGCCTCGAAGCCTTCATGTTGTCTGGAACAGGCTGG GCAGGGTTCCTGAAGCTGAAGACCTGGCCTCCCAGATGCTAAAAGGCTTTCCCAGAGACCGCGTCTCCGC CCAGGAAGCACTTGTTCATGATTATTTCAGCGCCCTGCCATCTCAGCTGTACCAGCTTCCTGATGAGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for mutant BC038807 unedited ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGGGAAAGGTTCAAGTG CGGGTTTTCTCCTTGAACCTAGAAGATTATGGGTCAAGAGCTGTGTGCAAAGACTGTACAGCCTGGATGC AGCTGCTACCATTGTTCAGAGGGAGGCGAGGCACACAGCTGTCGGAGGAGTCAGCCTGAGACCACGGAGG CTGCGTTCAAGCTAACAGACCTAAAAGAAGCATCATGTTCCATGACTTCATTTCACCCCAGGGGACTTCA AGCTGCCCCGTGCCCAGAAGTTCAAGAGTAAAAGGCCACGGAGTAACAGTTGATTTGTTTTTCAGGAAGA AGGATTCTGAGGCAGGTTTTTCAGTGGAAGGAAGAACCCTCCCCTTTTGGGGGCGGCCTTCTTCTTACTT TGAAACTGGGGGAAGCTTGGTTGAGGGCCCTTAATGCGCCAGTTTACAAGGGGGATAAGCCGAAATAAAA TGGCCCATTTGGGGCTTTAAGGTCCTACACCTTGATGCCAGAGGGAAGAGATTCCAATTACAGCATTCCA GAAACCTTCTCTCCGAGAGGGGTTTGAACCAGCCATATTTTGTTCTCCGCGTGTGATATATCCCCCAGAG ACACTGTCACTTTGTTTTGTGAATACTGCCAGAGACCTGCCATATATGTCTCATCTCGAGAGGCTTCTCC TAAGTGCAATTTCGTGTAATTTGGGGCGCTGGCTACTCCACCACAGTTTTAGAGGCCTGACTGAATTTAC TATAATCTGGAACATCAAGCGTAATTGGTTCGCGACCCTCTACCACGACCCTTCATATGTCACTTGCGAG CCCGAGTAGTCTGGGA Kinase domain sequence (showhide) >SC323533 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation ATKATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGGGAAAGGTTCAAGTGCGGG TTTTCTCCTTGAACCTAGAAGATTATGGGTCAAGAGCTGTGTGCAAAGACTGTACAGCCTGGATGCAGCT GCTACCATTGTTCAGAGGGAGGCGAGGCACACAGCTGTCGGAGGA
Restriction Sites	Please inquire
ACCN	BC038807
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC038807.1, AAH38807.1
RefSeq Size	2305 bp
RefSeq ORF	1050 bp
Locus ID	65061
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	Serine/threonine-protein kinase that acts like an antiapoptotic protein that counters TRAIL/TNFSF10-induced apoptosis by inducing phosphorylation of BIRC5 at 'Thr-34'.[UniProtKB/Swiss-Prot Function]

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