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TAOK1 (NM_020791) Human Untagged Clone
CAT＃: SC323537
TAOK1 (untagged)-Kinase deficient mutant (K57M) of Human TAO kinase 1 (TAOK1)
CNY 10,464.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hKFC-B; hTAOK1; KFC-B; MAP3K16; MARKK; PSK-2; PSK2; TAO1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323537 sequence for NM_020791 edited (data generated by NextGen Sequencing)
ATGCCATCAACTAACAGAGCAGGCAGCCTGAAGGACCCTGAAATTGCAGAGCTCTTCTTC AAAGAAGATCCAGAGAAGCTCTTCACAGATCTCAGAGAAATTGGCCATGGAAGCTTTGGA GCAGTGTATTTTGCACGAGATGTGCGTACCAATGAAGTGGTGGCCATCATGAAAATGTCT TATAGTGGAAAGCAGTCTACTGAGAAATGGCAGGATATTATTAAGGAAGTCAAGTTTCTA CAAAGAATAAAACATCCCAACAGTATAGAATACAAAGGCTGTTATTTACGTGAACACACA GCATGGCTTGTAATGGAATATTGTTTAGGATCTGCTTCGGATTTACTAGAAGTTCACAAA AAGCCATTACAAGAAGTGGAAATAGCAGCAATTACACATGGTGCTCTTCAGGGATTAGCC TACTTACATTCTCATACTATGATTCATAGAGATATCAAAGCAGGAAATATCCTTCTGACA GAACCAGGCCAGGTGAAACTTGCTGACTTTGGCTCTGCTTCCATGGCATCACCTGCCAAT TCCTTTGTGGGAACGCCGTATTGGATGGCCCCAGAAGTAATTTTAGCCATGGATGAAGGA CAATATGATGGCAAAGTAGATGTGTGGTCTCTTGGAATAACATGTATTGAACTAGCGGAA AGGAAGCCTCCTTTATTTAATATGAATGCAATGAGTGCATTATATCACATAGCCCAAAAT GAATCCCCTACACTACAGTCTAATGAATGGTCTGATTATTTTCGCAACTTTGTAGATTCT TGCCTCCAGAAAATCCCTCAAGATCGACCTACATCAGAGGAACTTTTAAAGCACATATTT GTTCTTCGGGAGCGCCCTGAAACCGTGTTAATAGATCTCATTCAGAGGACAAAGGATGCA GTAAGAGAGCTGGACAATCTGCAGTATCGAAAGATGAAGAAACTCCTTTTCCAGGAGGCA CATAATGGACCAGCAGTAGAAGCACAGGAAGAAGAAGAGGAACAAGATCATGGTGTTGGC CGGACAGGAACAGTTAATAGTGTTGGAAGTAATCAATCCATTCCCAGCATGTCCATCAGT GCCAGCAGCCAAAGCAGTAGTGTTAACAGTCTTCCAGATGTCTCAGATGACAAGAGTGAG CTAGACATGATGGAGGGAGACCACACAGTGATGTCTAACAGTTCTGTTATCCATTTAAAA CCAGAGGAAGAAAATTACAGAGAAGAGGGAGATCCTAGAACAAGAGCATCAGATCCACAA TCTCCACCCCAAGTATCTAGTCACAAATCACACTATCGTAATCGAGAACACTTTGCTACT ATACGGACAGCATCACTGGTTACGAGGCAAATGCAAGAACATGAGCAGGACTCTGAGCTT AGAGAACAAATGTCTGGCTATAAGCGAATGAGGCGACAACATCAAAAGCAACTGATGACT CTGGAAAACAAGCTAAAGGCTGAGATGGATGAACATCGCCTCAGATTAGACAAAGATCTT GAAACTCAGCGTAACAATTTTGCTGCAGAAATGGAGAAACTTATCAAGAAACACCAGGCT GCCATGGAGAAAGAGGCTAAAGTGATGTCCAATGAAGAGAAAAAATTTCAGCAACATATT CAGGCCCAACAGAAGAAAGAACTGAATAGTTTTCTCGAGTCCCAGAAAAGAGAGTATAAA CTTCGAAAAGAGCAGCTTAAAGAGGAGCTAAATGAAAACCAGAGTACCCCCAAAAAAGAA AAACAGGAGTGGCTTTCAAAGCAGAAGGAGAATATACAGCATTTCCAAGCAGAAGAAGAA GCTAACCTTCTTCGACGTCAAAGACAATACCTAGAGCTGGAATGCCGTCGCTTCAAGAGA AGAATGTTACTTGGGCGTCATAACTTAGAGCAGGACCTTGTCAGGGAGGAGTTAAACAAA AGACAGACTCAGAAGGACTTAGAGCATGCCATGCTACTCCGACAGCATGAATCTATGCAA GAACTGGAGTTCCGCCACCTCAACACAATTCAGAAGATGCGCTGTGAGTTGATCAGATTA CAGCATCAAACTGAGCTCACTAACCAGCTGGAATATAATAAGCGAAGAGAACGAGAACTA AGACGAAAGCATGTCATGGAAGTTCGACAACAGCCTAAGAGTTTGAAGTCTAAAGAACTC CAAATAAAAAAGCAGTTTCAGGATACCTGCAAAATCCAAACCAGACAGTACAAAGCATTA AGAAATCACCTGCTGGAGACTACACCAAAGAGTGAGCACAAAGCTGTTCTGAAACGGCTC AAGGAGGAACAGACCCGGAAATTAGCTATCTTGGCTGAGCAGTATGATCACAGCATTAAT GAAATGCTCTCCACACAAGCCCTGCGTTTGGATGAAGCACAGGAAGCAGAGTGCCAGGTT TTGAAGATGCAGCTGCAGCAGGAACTGGAGCTGTTGAATGCGTATCAGAGCAAAATCAAG ATGCAAGCTGAGGCACAACATGATCGAGAGCTTCGCGAGCTTGAACAGAGGGTCTCCCTC CGGAGGGCACTCTTAGAACAAAAGATTGAAGAAGAGATGTTGGCTTTGCAGAATGAGCGC ACAGAACGAATACGAAGCCTGTTGGAACGTCAAGCCAGAGAGATTGAAGCTTTTGACTCT GAAAGCATGAGACTAGGTTTTAGTAATATGGTCCTTTCTAATCTCTCCCCTGAGGCATTC AGCCACAGCTACCCGGGAGCTTCTGGTTGGTCACACAACCCTACTGGGGGTCCAGGACCT CACTGGGGTCATCCCATGGGTGGCCCACCACAAGCTTGGGGCCATCCAATGCAAGGTGGA CCCCAGCCATGGGGTCACCCTTCAGGGCCAATGCAAGGGGTACCTCGAGGTAGCAGTATG GGAGTCCGCAATAGCCCCCAGGCTCTGAGGCGGACAGCTTCTGGGGGACGGACGGAGCAG GGCATGAGCAGAAGCACGAGTGTCACTTCACAAATATCCAATGGGTCACACATGTCTTAT ACATAA Clone variation with respect to NM_020791.2 170 a=>t;699 c=>a;1279 c=>a >OriGene 5' read for mutant NM_020791 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCAACGTGACTTCA TTCATACAGATGAACCAAGGAACGGGATAGCAGTATAAAATTAGAATCAAGACAGCTGACTGCTCAGCAG GATGCCATCAACTAACAGAGCAGGCAGCCTGAAGGACCCTGAAATTGCAGAGCTCTTCTTCAAAGAAGAT CCAGAGAAGCTCTTCACAGATCTCAGAGAAAATTGGCCATGGAAGCTTTTGGAGCAGTGTATTTTTGCAC GAGATGTGCGTACCAATGAAGTGGTTGGCCATCATGAAAAAGGTCTTATAGTGGAAAGCAGTCTACTGGA GAAAATGCCAGGAAATTATTTAAGGAGGTCCAAGTTTTCTACAAGAATTAAAACCATCCCAAACGGTATA GAAAAAACAAAGGCTGGTTTATTTACTTGACCACAGGAATGCCTGGTATTGAAAATGGTTAAGAATCGGC TTCGAATTTCCAGAATTTACAAAAAGCCCTTTACAGGAGTGGGAATAGCGACATTTACCTGGGGGCTTCT CGGGTAATACCAACTCACTCTCTACCTGAGATTCAGAGATATTCAGCCGGAATTCCTCTGAGAAACCGCG CGTGTGACTGTGCGCTTGGCTCGTCTCGGGACACTGCGCATTCTTGGGAACCCTATGATAGGCCCGAGTA TTTTGCCTGTGTAGAGCAATATGTCGATAAGTGTGCCTTGGAAACCGATGACACCGAGAGACTCCTATTA TAATGCTGAGTCATATGCATGCGATAGTCTCACGCTTAAGGTCGGATTGCACCTGGTATCTGCC >SC323537 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TGAGWCAGAGAGCTCTTCACAGATCTCAGAGAAATTGGCCATGGAAGCTTTGGAGCAGTGTATTTTGCAC GAGATGTGCGTACCAATGAAGTGGTGGCCATCATGAAAATGTCTTATAGTGGAAAGCAGTCTACTGAGAA ATGGCAGGATATTATTAAGGAAGTCAAGTTTCTACAAAGAATAAAACATCCCAACAGTATAGAATACAAA GGCTGTTATTTACGTGAACACACAGCATGGCTTGTAATGGAATAT |
| Restriction Sites | Please inquire |
| ACCN | NM_020791 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020791.1, NP_065842.1 |
| RefSeq Size | 4662 bp |
| RefSeq ORF | 3006 bp |
| Locus ID | 57551 |
| UniProt ID | Q7L7X3 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219490 | TAOK1 (Myc-DDK-tagged)-Human TAO kinase 1 (TAOK1), transcript variant 1 |
CNY 10,456.00 |
|
| RC219490L1 | Lenti-ORF clone of TAOK1 (Myc-DDK-tagged)-Human TAO kinase 1 (TAOK1), transcript variant 1 |
CNY 12,856.00 |
|
| RC219490L2 | Lenti-ORF clone of TAOK1 (mGFP-tagged)-Human TAO kinase 1 (TAOK1), transcript variant 1 |
CNY 7,600.00 |
|
| RC219490L3 | Lenti-ORF clone of TAOK1 (Myc-DDK-tagged)-Human TAO kinase 1 (TAOK1), transcript variant 1 |
CNY 7,600.00 |
|
| RC219490L4 | Lenti-ORF clone of TAOK1 (mGFP-tagged)-Human TAO kinase 1 (TAOK1), transcript variant 1 |
CNY 12,856.00 |
|
| RG219490 | TAOK1 (tGFP-tagged) - Human TAO kinase 1 (TAOK1), transcript variant 1 |
CNY 12,056.00 |
|
| SC309825 | TAOK1 (untagged)-Human TAO kinase 1 (TAOK1), transcript variant 1 |
CNY 10,464.00 |
