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GSK3 beta (GSK3B) (NM_002093) Human Untagged Clone
CAT＃: SC323589
GSK3B (untagged)-Kinase deficient mutant (K85M) of Human glycogen synthase kinase 3 beta (GSK3B)
CNY 3,656.00
CNY 6,940.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323589 sequence for NM_002093 edited (data generated by NextGen Sequencing)
ATGTCAGGGCGGCCCAGAACCACCTCCTTTGCGGAGAGCTGCAAGCCGGTGCAGCAGCCT TCAGCTTTTGGCAGCATGAAAGTTAGCAGAGACAAGGACGGCAGCAAGGTGACAACAGTG GTGGCAACTCCTGGGCAGGGTCCAGACAGGCCACAAGAAGTCAGCTATACAGACACTAAA GTGATTGGAAATGGATCATTTGGTGTGGTATATCAAGCCAAACTTTGTGATTCAGGAGAA CTGGTCGCCATCAAGAAAGTATTGCAGGACAAGAGATTTAAGAATCGAGAGCTCCAGATC ATGAGAAAGCTAGATCACTGTAACATAGTCCGATTGCGTTATTTCTTCTACTCCAGTGGT GAGAAGAAAGATGAGGTCTATCTTAATCTGGTGCTGGACTATGTTCCGGAAACAGTATAC AGAGTTGCCAGACACTATAGTCGAGCCAAACAGACGCTCCCTGTGATTTATGTCAAGTTG TATATGTATCAGCTGTTCCGAAGTTTAGCCTATATCCATTCCTTTGGAATCTGCCATCGG GATATTAAACCGCAGAACCTCTTGTTGGATCCTGATACTGCTGTATTAAAACTCTGTGAC TTTGGAAGTGCAAAGCAGCTGGTCCGAGGAGAACCCAATGTTTCGTATATCTGTTCTCGG TACTATAGGGCACCAGAGTTGATCTTTGGAGCCACTGATTATACCTCTAGTATAGATGTA TGGTCTGCTGGCTGTGTGTTGGCTGAGCTGTTACTAGGACAACCAATATTTCCAGGGGAT AGTGGTGTGGATCAGTTGGTAGAAATAATCAAGGTCCTGGGAACTCCAACAAGGGAGCAA ATCAGAGAAATGAACCCAAACTACACAGAATTTAAATTCCCTCAAATTAAGGCACATCCT TGGACTAAGGATTCGTCAGGAACAGGACATTTCACCTCAGGAGTGCGGGTCTTCCGACCC CGAACTCCACCGGAGGCAATTGCACTGTGTAGCCGTCTGCTGGAGTATACACCAACTGCC CGACTAACACCACTGGAAGCTTGTGCACATTCATTTTTTGATGAATTACGGGACCCAAAT GTCAAACTACCAAATGGGCGAGACACACCTGCACTCTTCAACTTCACCACTCAAGAACTG TCAAGTAATCCACCTCTGGCTACCATCCTTATTCCTCCTCATGCTCGGATTCAAGCAGCT GCTTCAACCCCCACAAATGCCACAGCAGCGTCAGATGCTAATACTGGAGACCGTGGACAG ACCAATAATGCTGCTTCTGCATCAGCTTCCAACTCCACCTGA Clone variation with respect to NM_002093.3 >OriGene 5' read for mutant NM_002093 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAAATCATCTATAT GTTAAATATCCGTGCCGAACTGTCTTGAAGGAGAAATATATCGCTTGTTTTGTTTTTTATAGTATACAAA AGGAGTGAAAAGCCAAGAGGACGAAGTCTTTTTCTTTTTCTTCTGTGGGAGAACTTAATGCTGCATTTAT CGTTAACCTAACACCCCAACATAAAGACAAAAGGGAAGAAAAAGGAGGGAAGGAAGGAAAAGGTGATTCG CGAAGAGAGTGATCATGTCAGGGCGGCCCAGACCCACCCTCCTTTGCGGAGAGCTTGCCAGGCCCGGTTG GCCAGCAAGCCTTTCAGCTTTTTGCGCACCATGAAAAGTTAGCGCGAAAAACAAGGAACGGACGGCAGGT GTGACAACGTGGTGTGGCAACTCTGGGGCAGGGTCAGAGACAGGCCAAAGAAGGTCGGCTACACAGAACC TAAAGTTGATTGAAATGGATCATGGGGTGGGGTATCCAGCCCAAATTTTGAGATTCGGGAAATGGGTTCC CATTAGGAGTATTTTGCGAGAAGAGATATAGAAAATCGAACTCTCAGATTGTAAAAACCTATCTCACTGA ACCATGCCCGAGTGGTTTTTTCTCCTACTCCCCCTGTGGGAAGAAAAAAGAAGAGGTTCATTATACTGTG GTGCGAGATGTGCTGCAAAAACTATTATAGTTGTCGCACACCTATTGTGACACCAACGCCCCCTCGGGTA TGTAGCTGTTAGTATGACTCGTTCGCAGATATCCACTACATCTCCTAAGACTCGGCGTATACCCGACCCC CTGTATCATCAGCGCGTTATACTGCAACTTGCCAGCACGCTCTGAGACACACGCGTTATAAGTATGCGTG >SC323589 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAAATCATCTATATGTTA AATATCCGTGCCGATCTGTCTTGAAGGAGAAATATATCGCTTGTTTTGTTTTTTATAGTATACAAAAGGA GTGAAAAGCCAAGAGGACGAAGTCTTTTTCTTTTTCTTCTGTGGG |
| Restriction Sites | Please inquire |
| ACCN | NM_002093 |
| Insert Size | 1680 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002093.2, NP_002084.2 |
| RefSeq Size | 1639 bp |
| RefSeq ORF | 1302 bp |
| Locus ID | 2932 |
| UniProt ID | P49841 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Alzheimer's disease, Axon guidance, Basal cell carcinoma, B cell receptor signaling pathway, Cell cycle, Chemokine signaling pathway, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Hedgehog signaling pathway, Insulin signaling pathway, Melanogenesis, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, T cell receptor signaling pathway, Wnt signaling pathway |
| Gene Summary | The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200468 | GSK3B (Myc-DDK-tagged)-Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1 |
CNY 3,656.00 |
|
| RC200468L1 | Lenti ORF clone of Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200468L2 | Lenti ORF clone of Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200468L3 | Lenti ORF clone of Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200468L4 | Lenti ORF clone of Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG200468 | GSK3B (tGFP-tagged) - Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1 |
CNY 5,256.00 |
|
| SC118826 | GSK3B (untagged)-Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1 |
CNY 3,656.00 |
|
| SC317704 | GSK3B (untagged)-Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1 |
CNY 3,656.00 |
|
| SC319432 | GSK3B (untagged)-Human glycogen synthase kinase 3 beta (GSK3B), transcript variant 1 |
CNY 3,656.00 |
|
| SC323706 | GSK3B (untagged)-Kinase deficient mutant (K85M) of Human glycogen synthase kinase 3 beta (GSK3B) |
CNY 6,940.00 |
|
| SC323707 | GSK3B (untagged)-Kinase deficient mutant (K85M) of Human glycogen synthase kinase 3 beta (GSK3B) |
CNY 6,940.00 |
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