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HCK (NM_002110) Human Untagged Clone
CAT＃: SC323684
HCK (untagged)-Kinase deficient mutant (K269M) of Human hemopoietic cell kinase (HCK)
CNY 3,768.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | JTK9; p59Hck; p61Hck |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002110, the custom clone sequence may differ by one or more nucleotides
CTGGGGGGGCGCTCAAGCTGCGAGGATCCGGGCTGCCCGCGAGACGAGGAGCGGGCGCCCAGGATGGGGT GCATGAAGTCCAAGTTCCTCCAGGTCGGAGGCAATACATTCTCAAAAACTGAAACCAGCGCCAGCCCACA CTGTCCTGTGTACGTGCCGGATCCCACATCCACCATCAAGCCGGGGCCTAATAGCCACAACAGCAACACA CCAGGAATCAGGGAGGCAGGCTCTGAGGACATCATCGTGGTTGCCCTGTATGATTACGAGGCCATTCACC ACGAAGACCTCAGCTTCCAGAAGGGGGACCAGATGGTGGTCCTAGAGGAATCCGGGGAGTGGTGGAAGGC TCGATCCCTGGCCACCCGGAAGGAGGGCTACATCCCAAGCAACTATGTCGCCCGCGTTGACTCTCTGGAG ACAGAGGAGTGGTTTTTCAAGGGCATCAGCCGGAAGGACGCAGAGCGCCAACTGCTGGCTCCCGGCAACA TGCTGGGCTCCTTCATGATCCGGGATAGCGAGACCACTAAAGGAAGCTACTCTTTGTCCGTGCGAGACTA CGACCCTCGGCAGGGAGATACCGTGAAACATTACAAGATCCGGACCCTGGACAACGGGGGCTTCTACATA TCCCCCCGAAGCACCTTCAGCACTCTGCAGGAGCTGGTGGACCACTACAAGAAGGGGAACGACGGGCTCT GCCAGAAACTGTCGGTGCCCTGCATGTCTTCCAAGCCCCAGAAGCCTTGGGAGAAAGATGCCTGGGAGAT CCCTCGGGAATCCCTCAAGCTGGAGAAGAAACTTGGAGCTGGGCAGTTTGGGGAAGTCTGGATGGCCACC TACAACAAGCACACCAAGGTGGCAGTGAAGACGATGAAGCCAGGGAGCATGTCGGTGGAGGCCTTCCTGG CAGAGGCCAACGTGATGAAAACTCTGCAGCATGACAAGCTGGTCAAACTTCATGCGGTGGTCACCAAGGA GCCCATCTACATCATCACGGAGTTCATGGCCAAAGGAAGCTTGCTGGACTTTCTGAAAAGTGATGAGGGC AGCAAGCAGCCATTGCCAAAACTCATTGACTTCTCAGCCCAGATTGCAGAAGGCATGGCCTTCATCGAGC AGAGGAACTACATCCACCGAGACCTCCGAGCTGCCAACATCTTGGTCTCTGCATCCCTGGTGTGTAAGAT TGCTGACTTTGGCCTGGCCCGGGTCATTGAGGACAACGAGTACACGGCTCGGGAAGGGGCCAAGTTCCCC ATCAAGTGGACAGCTCCTGAAGCCATCAACTTTGGCTCCTTCACCATCAAGTCAGACGTCTGGTCCTTTG GTATCCTGCTGATGGAGATCGTCACCTACGGCCGGATCCCTTACCCAGGGATGTCAAACCCTGAAGTGAT CCGAGCTCTGGAGCGTGGATACCGGATGCCTCGCCCAGAGAACTGCCCAGAGGAGCTCTACAACATCATG ATGCGCTGCTGGAAAAACCGTCCGGAGGAGCGGCCGACCTTCGAATACATCCAGAGTGTGCTGGATGACT TCTACACGGCCACAGAGAGCCAGTACCAACAGCAGCCATGA >OriGene 5' read for mutant NM_002110 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGCTGCCCGCG AGACGAGGAGCGGGCGCCCAGGATGGGGTGCATGAAGTCCAAGTTCCTCCAGGTCGGAGGCAATACATTC TCAAAAACTGAAACCAGCGCCAGCCCACACTGTCCTGTGTACGTGCCGGATCCCACATCCACCATCAAGC CGGGGCCTAATAGCCACAACAGCAACACACCAGGAATCAGGGAGGCAGGCTCTGAGGACATCATCGGTGG TTGCCCTGTATGATTACGAGGCCATTCACCCACGAAGGACCTCAGCTTCCAGAAAGGGGGACCCAGATGG TGGTCCCTAGAGAAATCCCGGGGAGTGGTGGGAGGCTTCGATCCCCTGGCCACCCGGAAAGGAGGGCTAA CATCCCAGCAACTATGTCGCCCGCGTGACTCTCTGGAAACCAGAGGAGTGGTTTTTTCAAGGCATCAGCC GGAAGGACCAAACGCCAATGGCTGGTCCCGCCACAGGCTGGCTTCTCTGATCGGGATAGCAGACCACTAA GGAACTCTCTTGGCTGTGCAACTCGCACTTGGAGGGAAATCGGGACATTACATTCGAACCTGCACAGGGC TTACATATCCCGAGCCTTCAACTTGAGAGCGGGGACAATCAAAGGAACAGGCTTGCAACTTGTGCCGAGT TCAGTAACTTGAAACCGATCCTGGATTCTTCGGAAACGTTCCCGCCTTGGACTTGAATCTACAGCCAATG GGTGA >SC323684 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TMKGRTAGACTTGGAGCTGGGCAGTTTGGGGAAGTCTGGATGGCCACCTACAACAAGCACACCAAGGTGG CAGTGATGACGATGAAGCCAGGGAGCATGTCGGTGGAGGCCTTCCTGGCAGAGGCCAACGTGATGAAAAC TCTGCAGCATGACAAGCTGGTCAAACTTCATGCGGTGGTCACCAAGGAGCCCATCTACATCATCACGGAG TTCATGGCCAAAGGAAGCTTGCTGGACTTTCTGAAAAGTGATGAG |
| Restriction Sites | Please inquire |
| ACCN | NM_002110 |
| Insert Size | 1790 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002110.1, NP_002101.1 |
| RefSeq Size | 2015 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 3055 |
| UniProt ID | P08631 |
| Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Chemokine signaling pathway, Fc gamma R-mediated phagocytosis |
| Gene Summary | The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010] Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons, as demonstrated in PMIDs 1875927 and 7791757. The longer isoform (a, also known as p61HCK) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (b, also known as p59HCK) is derived from a downstream AUG start codon. The longer isoform (a) is represented in this RefSeq. CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1, BC108930.1 and others, represents a long human HCK isoform, known as p61HCK, as described in PMIDs 1875927 and 7791757. This isoform initiates translation from a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak signal context. Alternative translation initiation from a downstream AUG start codon produces an isoform that is 21 aa shorter at the N-terminus. The shorter isoform, which is known as p59HCK, is represented by CCDS 54455.1. These isoforms exhibit distinct subcellular distributions, as indicated in PMID:7791757. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217022 | HCK (Myc-DDK-tagged)-Human hemopoietic cell kinase (HCK), transcript variant 1 |
CNY 3,920.00 |
|
| RC217022L1 | Lenti ORF clone of Human hemopoietic cell kinase (HCK), transcript variant 1, Myc-DDK-tagged |
CNY 6,320.00 |
|
| RC217022L2 | Lenti ORF clone of Human hemopoietic cell kinase (HCK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC217022L3 | Lenti ORF clone of Human hemopoietic cell kinase (HCK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217022L4 | Lenti ORF clone of Human hemopoietic cell kinase (HCK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217022 | HCK (tGFP-tagged) - Human hemopoietic cell kinase (HCK), transcript variant 1 |
CNY 4,370.00 |
|
| SC118838 | HCK (untagged)-Human hemopoietic cell kinase (HCK), transcript variant 1 |
CNY 3,768.00 |
|
| SC317892 | HCK (untagged)-Human hemopoietic cell kinase (HCK), transcript variant 1 |
CNY 3,928.00 |
