ARHGAP21 (NM_020824) Human Untagged Clone

CAT#: SC328093

ARHGAP21 (untagged)-Human Rho GTPase activating protein 21 (ARHGAP21)



  "NM_020824" in other vectors (3)

CNY 37,810.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ARHGAP10
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_020824, the custom clone sequence may differ by one or more nucleotides
ATGATGGCCACGCGTCGGACTGGTCTGTCTGAGGGAGATGGTGACAAGCTCAAGGCCTGC
GAGGTCTCAAAAAATAAAGATGGAAAAGAACAAAGTGAAACTGTATCACTGTCTGAAGAT
GAAACATTCTCCTGGCCAGGTCCCAAAACAGTTACGTTGAAAAGAACATCTCAAGGCTTT
GGTTTTACATTAAGACATTTTATTGTTTATCCCCCAGAGTCTGCAATTCAATTTTCATAT
AAGGATGAAGAAAATGGAAACAGAGGAGGAAAACAAAGAAACCGCTTGGAACCAATGGAT
ACCATATTTGTTAAGCAAGTTAAAGAAGGAGGACCTGCTTTTGAAGCTGGATTATGTACA
GGTGACCGAATTATAAAAGTCAATGGAGAAAGTGTTATTGGCAAAACCTATTCCCAAGTA
ATTGCTTTAATTCAAAACAGTGATACAACATTGGAACTTAGTGTTATGCCAAAAGATGAA
GACATTCTCCAAGTGCTACAGTTTACAAAGGATGTCACAGCACTGGCATATTCTCAAGAT
GCCTACCTGAAAGGCAACGAAGCTTATAGCGGCAATGCCCGCAATATACCTGAACCTCCA
CCAATCTGCTATCCCTGGCTGCCATCTGCCCCATCAGCCATGGCACAGCCAGTTGAAATA
TCTCCTCCTGACTCATCATTGAGCAAACAGCAAACCAGTACACCAGTACTGACACAACCT
GGTAGGGCCTATAGAATGGAAATACAAGTGCCTCCATCACCAACAGATGTTGCAAAATCA
AACACAGCAGTGTGTGTTTGCAATGAAAGTGTAAGGACTGTCATTGTGCCTTCTGAGAAG
GTTGTAGATTTGTTATCCAATAGAAACAACCATACAGGTCCTTCACATAGAACTGAAGAA
GTGAGGTATGGCGTGAGTGAGCAGACCTCTTTAAAAACAGTGTCAAGAACCACATCACCA
CCATTATCAATTCCCACCACTCATCTAATTCATCAGCCTGCAGGCTCCAGATCACTGGAA
CCTTCTGGAATTTTACTTAAGTCTGGAAATTACAGTGGACATTCTGATGGAATCTCAAGC
AGCAGATCTCAAGCTGTGGAGGCTCCCTCTGTATCTGTTAATCACTATTCGCCAAATTCC
CATCAGCACATAGACTGGAAAAACTATAAAACTTACAAAGAGTATATTGATAACAGACGA
TTGCACATAGGTTGTCGGACAATACAAGAAAGATTAGATAGTTTAAGAGCAGCATCTCAA
AGCACGACAGATTATAACCAGGTCGTCCCCAACCGCACTACTTTGCAGGGACGACGTCGA
AGCACCTCTCATGATCGAGTGCCCCAGTCTGTCCAGATACGGCAACGCAGTGTGTCCCAA
GAAAGACTGGAAGATTCTGTGCTAATGAAGTATTGTCCAAGAAGTGCATCTCAAGGAGCA
CTGACGTCTCCATCTGTTAGTTTTAGTAATCATAGAACTCGTTCATGGGATTATATTGAG
GGACAGGATGAAACCTTAGAAAATGTCAATTCTGGAACTCCAATACCTGATTCCAATGGA
GAGAAAAAACAGACTTACAAGTGGAGTGGGTTTACTGAACAGGATGATAGACGAGGTATT
TGTGAAAGACCTAGGCAGCAAGAAATTCATAAATCTTTTCGAGGTTCCAATTTTACTGTG
GCTCCAAGCGTTGTTAATTCTGATAACAGGCGAATGAGTGGTAGAGGAGTGGGATCTGTG
TCGCAGTTTAAAAAAATTCCACCAGATCTAAAAACATTGCAGTCAAACAGAAATTTTCAG
ACTACTTGTGGAATGTCACTGCCTCGGGGTATTTCACAAGACAGGTCACCTCTTGTGAAA
GTCCGAAGTAATTCTCTGAAAGCTCCTTCCACGCATGTCACAAAACCATCATTTAGCCAG
AAATCATTTGTTTCTATCAAAGACCAAAGACCAGTAAATCACTTGCATCAGAACAGTCTG
TTGAATCAGCAGACATGGGTAAGGACTGACAGTGCCCCCGATCAGCAAGTGGAGACTGGG
AAATCCCCCTCTTTATCTGGAGCCTCTGCCAAGCCTGCCCCTCAGTCGAGTGAAAACGCT
GGTACTTCAGATTTAGAACTACCTGTCAGTCAAAGGAATCAAGATTTAAGTTTACAAGAG
GCTGAAACTGAGCAATCAGATACTTTAGATAATAAAGAAGCTGTCATCCTAAGGGAAAAA
CCTCCATCTGGACGCCAGACACCGCAGCCTTTAAGGCATCAGTCTTACATCTTGGCAGTA
AATGACCAGGAGACCGGGTCAGACACTACCTGCTGGCTGCCCAATGATGCACGTCGAGAG
GTCCACATAAAAAGAATGGAGGAAAGAAAAGCCTCGAGTACCAGTCCGCCTGGCGATTCT
TTGGCTTCCATCCCATTTATAGATGAACCAACTAGCCCTAGCATTGATCATGATATTGCA
CATATCCCTGCCTCTGCTGTTATATCAGCCTCTACCTCTCAGGTCCCCTCCATAGCAACA
GTTCCTCCTTGCCTCACAACTTCAGCTCCATTAATTCGCCGTCAGCTCTCACATGACCAC
GAATCTGTTGGCCCTCCTAGCCTGGATGCTCAGCCCAACTCAAAGACAGAAAGATCAAAA
TCATATGATGAGGGTCTGGATGATTACAGAGAAGATGCAAAATTGTCCTTTAAGCACGTA
TCTAGTCTGAAGGGAATCAAGATCGCAGACAGCCAAAAGTCATCAGAAGACTCTGGGTCC
AGAAAAGATTCTTCCTCAGAGGTCTTCAGTGATGCTGCCAAGGAAGGGTGGCTTCATTTC
CGACCCCTTGTCACCGATAAGGGCAAGCGAGTTGGTGGAAGTATTCGGCCATGGAAACAG
ATGTATGTTGTCCTTCGGGGTCATTCACTTTACCTGTACAAAGATAAAAGAGAGCAGACG
ACTCCGTCTGAGGAAGAGCAGCCCATCAGTGTTAATGCTTGCTTGATAGACATCTCTTAC
AGTGAGACCAAGAGGAAAAATGTGTTTCGACTCACCACGTCCGACTGTGAATGCCTGTTT
CAGGCTGAAGACAGAGATGATATGCTAGCTTGGATCAAGACGATCCAGGAGAGCAGCAAC
CTAAACGAAGAGGACACTGGAGTCACTAACAGGGATCTAATTAGTCGAAGAATAAAAGAA
TACAACAATCTGATGAGCAAAGCAGAACAGTTGCCAAAAACACCTCGCCAGAGTCTCAGC
ATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACACTCTCCG
AAGGAAGAGTCGGAAAGGAAACTTCTCAGTAAAGATGATACCAGTCCCCCAAAAGACAAA
GGCACATGGAGAAAAGGCATTCCAAGTATCATGAGAAAGACATTTGAGAAAAAGCCAACT
GCTACAGGAACTTTCGGCGTCCGACTAGATGACTGCCCACCAGCTCATACTAATCGGTAT
ATTCCATTAATAGTTGACATATGTTGCAAATTAGTTGAAGAAAGAGGTCTTGAATATACA
GGTATTTATAGAGTTCCTGGAAATAATGCAGCCATCTCAAGTATGCAAGAAGAACTCAAC
AAGGGAATGGCTGATATTGATATACAAGATGATAAATGGCGAGATTTGAATGTGATAAGC
AGTTTACTAAAATCCTTCTTCAGAAAACTCCCTGAGCCTCTCTTCACAAATGATAAATAT
GCTGATTTTATTGAAGCCAATCGTAAAGAAGATCCTCTAGATCGTCTGAAAACATTAAAA
AGACTAATTCACGATTTGCCTGAACATCATTATGAAACACTTAAGTTCCTTTCAGCTCAT
CTGAAGACAGTGGCAGAAAATTCAGAAAAAAATAAGATGGAACCAAGAAACCTAGCAATA
GTGTTTGGTCCCACCCTTGTTCGAACATCAGAAGACAACATGACCCACATGGTCACCCAC
ATGCCTGACCAGTACAAGATTGTAGAAACGCTCATCCAGCACCATGACTGGTTTTTCACA
GAAGAAGGTGCTGAAGAGCCTCTTACAACAGTGCAGGAGGAAAGCACAGTAGACTCCCAG
CCAGTGCCAAACATAGATCATTTACTCACCAACATTGGAAGGACAGGAGTCTCCCCAGGA
GATGTATCAGATTCAGCTACTAGTGACTCAACAAAATCTAAGGGTTCTTGGGGATCTGGA
AAGGATCAGTATAGCAGGGAACTGCTTGTGTCCTCCATCTTTGCAGCTGCTAGTCGCAAG
AGGAAGAAGCCGAAAGAAAAAGCACAGCCTAGCAGCTCAGAAGATGAACTGGACAATGTA
TTTTTTAAGAAAGAAAATGTGGAACAGTGTCACAATGATACTAAAGAGGAGTCCAAAAAA
GAAAGTGAGACACTGGGCAGAAAACAGAAGATCATCATTGCCAAAGAAAACAGCACTAGG
AAAGACCCCAGCACGACAAAAGATGAAAAGATATCACTAGGAAAAGAGAGCACGCCTTCT
GAAGAACCCTCACCACCACACAACTCAAAACACAACAAGTCACCAACTCTCAGCTGTCGC
TTTGCCATCCTGAAAGAGAGCCCCAGGTCACTTCTGGCACAGAAGTCCTCCCACCTTGAA
GAGACAGGCTCTGACTCTGGCACTTTGCTCAGCACGTCTTCCCAGGCCTCCCTGGCAAGG
TTTTCCATGAAGAAATCAACCAGTCCAGAAACGAAACATAGCGAGTTTTTGGCCAACGTC
AGCACCATCACCTCAGATTATTCCACCACATCGTCTGCTACATACTTGACTAGCCTGGAC
TCCAGTCGACTGAGCCCTGAGGTGCAATCCGTGGCAGAGAGCAAGGGGGACGAGGCAGAT
GACGAGAGAAGCGAACTCATCAGTGAAGGGCGGCCTGTGGAAACCGACAGCGAGAGCGAG
TTTCCCGTGTTCCCCACAGCCTTGACTTCAGAGAGGCTTTTCCGAGGAAAACTGCAAGAA
GTGACTAAGAGCAGCCGGAGAAATTCTGAAGGAAGTGAATTAAGTTGCACCGAGGGAAGT
TTAACATCAAGTTTAGATAGCCGGAGACAGCTCTTCAGTTCCCATAAACTCATCGAATGT
GATACTCTTTCCAGGAAAAAATCAGCTAGATTCAAGTCAGATAGTGGAAGTCTAGGAGAT
GCCAAGAATGAGAAAGAAGCACCTTCGTTAACTAAAGTGTTTGATGTTATGAAAAAAGGA
AAGTCAACTGGGAGTTTACTGACACCCACCAGAGGCGAATCCGAAAAACAGGAACCCACA
TGGAAAACGAAAATAGCAGATCGGTTAAAACTGAGACCCAGAGCCCCTGCGGATGACATG
TTTGGAGTAGGGAATCACAAAGTGAATGCCGAGACTGCTAAAAGGAAAAGCATCCGGCGC
AGACATACACTAGGAGGGCACAGAGATGCTACCGAAATCAGCGTTTTGAATTTTTGGAAA
GTGCATGAGCAGAGCGGGGAGAGAGAATCTGAACTTTCAGCTGTAAACCGGTTAAAACCA
AAATGCTCAGCCCAGGACCTTTCCATCTCAGACTGGCTGGCCAGGGAACGCCTACGCACC
AGTACCTCTGACCTTAGCAGAGGAGAAATCGGAGATCCCCAGACAGAGAACCCAAGCACA
CGAGAAATAGCCACGACCGACACACCTTTGTCTCTTCATTGCAACACAGGCAGTTCTTCC
AGCACCTTGGCTTCAACAAACAGGCCCCTTCTTTCCATACCACCACAGTCACCTGACCAA
ATAAACGGAGAAAGCTTCCAGAACGTGAGCAAAAATGCTAGTTCTGCAGCGAATGCCCAA
CCTCATAAACTGTCTGAAACCCCAGGCAGTAAAGCAGAGTTTCATCCCTGTCTT
Restriction Sites Please inquire     
ACCN NM_020824
Insert Size 7185 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020824.3, NP_065875.3
RefSeq Size 7185 bp
RefSeq ORF 7185 bp
Locus ID 57584
UniProt ID Q5T5U3
Domains RhoGAP, PDZ, PH
Gene Summary ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3 complex (MIM 604221) and F-actin dynamics at the Golgi through control of CDC42 activity (Dubois et al., 2005 [PubMed 15793564]).[supplied by OMIM, Mar 2008]
Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: The 5'-most in-frame translation start codon is selected for this RefSeq and is well-conserved among mammalian species. An alternative start codon that would reduce the protein length by 1 aa is also present. The use of the downstream start codon is assumed in the literature, including PMIDs:12056806, 15793564 and 17347647.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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