PMF1 (NM_001199653) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331335 representing NM_001199653. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCGAAGCAAGTAGCGCCAATCTAGGCAGCGGCTGTGAGGAAAAAAGGCATGAGGGGTCGTCTTCG GAATCTGTGCCACCCGGCACTACCATTTCGAGGGTGAAGCTCCTCGACACCATGGTGGACACTTTTCTT CAGAAGCTGGTCGCCGCCGGCAGCTACCAGAGATTCACTGACTGCTATAAGTGCTTCTACCAGTTGCAG CCTGCGATGACACAGCAAATCTATGACAAGTTTATAGCTCAGTTGCAGACATCTATCCGGGAGGAAATC TCTGACATCAAAGAGGAGGGGAACCTAGAAGCTGTCTTGAATGCCTTGGATAAAATTGTGGAAGAAGGC AAAGTCCGCAAAGAGCCAGCCTGCAACGGGACACCCTGCGGCGCCATGTGCAGAAACAGGAGGCCGAGA ACCAGCAGCTGGCAGATGCCGTCCTGGCAGGGCGGAGGCAGGTGGAGGAGCTGCAGCTACAGGTCCAGG CCCAGCAGCAGGCCTGGCAGGCTCTACACAGAGAACAGAGGGAGCTGGTTGCTGTGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199653
Insert Size	543 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199653.1
RefSeq Size	1061 bp
RefSeq ORF	543 bp
Locus ID	11243
UniProt ID	Q6P1K2
Protein Families	Stem cell - Pluripotency
MW	20.2 kDa
Gene Summary	Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate exon but includes another in-frame exon in the 5' coding region, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The resulting isoform (3) has a distinct C-terminus and is shorter than isoform 1.

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