Smek1 (PPP4R3A) (NM_001284280) Human Untagged Clone

CAT#: SC337527

SMEK1 (untagged) - Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1), transcript variant 3



  "NM_001284280" in other vectors (2)

CNY 6,616.00

CNY 7,890.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
SMEK1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms FLFL1; KIAA2010; MSTP033; PP4R3; PP4R3A; SMEK1; smk-1; smk1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_032560 edited
CGCCCCTCGCCTCTCCAGGGCCCCTTTCCTGGGCGTCTACTGGCGGGGCCCCCGCCCCGG
TTCCCGGGCGGCACGATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGAG
GACCGGCAGTGGGACGACCGGGGCACCGGGCATGTGTCGTCTGGCTACGTGGAGCGGCTG
AAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGACGGTTCTCTACTTTTAGAGTCGAAA
ATAAATCCTAACACTGCATACCAGAAACAACAGGACACTCTGATTGTGTGGTCTGAAGCA
GAAAATTATGACTTGGCCCTTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTTGGGAG
AAAATATGTCAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGAT
GAATCTGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAATTGCCATCTTGT
GAATTAAGTCGCCTTGAAGAAATTGCAGAACTTGTGGCATCATCTTTACCTTCACCTCTT
CGTCGTGAAAAACTTGCACTGGCACTAGAAAATGAGGGTTATATTAAAAAGCTCCTGGAG
CTTTTTCATGTGTGTGAAGATTTGGAAAATATTGAAGGACTGCACCACTTGTATGAAATT
ATCAAAGGCATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTTATGTTCTCTGAAGAA
TGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCACAACCACGAAAA
CACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAAGTGATTCCCATATCAGATCCT
GAGCTGAAACAAAAAATTCATCAGACATACAGAGTTCAGTATATACAAGATATGGTTCTA
CCAACTCCTTCGGTCTTTGAAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTC
AATAAGGTAGAGATTGTTGGCATGTTGCAGGAAGATGAAAAATTTCTGACAGATTTGTTT
GCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTGGTTAACTTTTTA
AAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAACAGAGATGCTTTTTTCAAG
ACTTTGTCAAACATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATGATACA
CAGGTGCGAAGTGCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATG
GTACGAGAGTTTGTCATGCAGGAGGCACAACAGAATGATGATGATATTTTGCTCATCAAC
CTCATTATAGAACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTT
ATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGCCAATAAAACA
GAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTATGCATGTTCTCACTGCTCCT
TTACTAGCAAATACAACAGAAGACAAACCTAGTAAAGATGATTTTCAGACTGCCCAACTA
TTGGCACTTGTATTGGAATTGTTAACATTTTGTGTGGAGCACCATACCTACCACATAAAG
AACTACATTATTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCAT
GCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATTAAAAGATGAG
TTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAACCAGTAGTGAAAGCATTTCTC
AACAATGGATCCCGCTACAATCTGATGAACTCTGCCATAATAGAGATGTTTGAATTTATT
AGAGTGGAAGATATAAAATCATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTG
GAAGATGTAGATTATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGA
GAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAATCACAGATAT
CGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGTGGTTTAACACAGATGAAGAT
GACATGGAAGATGGAGAAGCTGTAGTGTCTCCATCTGACAAAACTAAAAATGATGATGAT
ATTATGGATCCAATAAGTAAATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAG
GAAGTGCTTCTGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTG
TCCAGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACAACAAATCTGCCTGGTTCT
CCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTGGATCCGTACCTAAAAATACA
TCTCAGACGGCAGCTATTACTACAAAGGGAGGCCTCGTGGGTCTGGTAGATTATCCTGAT
GATGATGAAGATGATGATGAGGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAA
GCAAAATTTGATTCATAATAATGGCAACGGCCTAGGATCAGTACCTGTTGAAAAAAACTG
GTTCTCCACCCCTCCCCCATACAAAATCCACAAAAAAGCGCAGTGGTCTCTTGTGAATGA
CTGACACAGATCAGCCTCTTACACTTGACTTCTGCTCATCAAGTGCCAATTCAATGGAGC
AGGAGGAGGGGATATCATATATTTAGGGGAAAGACTTAAGCCTTTGAGCTCTCCAGCTTG
GACCACACATTGCCCTTTTCTCAGGGAAGGAAATGGAAACAAAAAGCCAACAGGGCAGGG
GTTTTGTAAGTGGAACTCTGGATTGACTGGTCAGTTGCTACAATCAGAATATGCTTTCTT
GGACCATGTTTGAGACTCAGAAGAATGGGCCTTTCTGCCATAATTCTTCACTAGTCAAGA
ATGCCAGCAGTTTCTTTGTATAAAGAGACCTGCCTTTAAAATCATACATTCTGAACATTT
TAGTCAAGCTACAACAGGTTTGGAAAACCTCTGTGGGGGAGGGGCGAGTATAAAGTTTTC
CTCTTTTTTAACTGTTCCCTTTGCCCTTCAAACTGCAGATATTTTTTTTTTTAAGTGGGG
ACTTCTCCCTACTTGATTAAAGATTGAGTGGAATTCTAGATGTGGTCATTTGTGTCATAA
TTTTTTTGTTTTATTTTGTTTTTGATTTTTTTTTTCCTCCCCTGAGTGTATGCTTAGTTG
TTGAGTATATATATTTGGGACCATTAAAACTTTTTTTGATGTAATATAACCTAACGTTGT
GCTGGTACCTGTTTTACCATGTGTAATTTTTGTTCTACATCACAGTTCTTAATTTGTTTA
GAGTTTTATGAAAGATGGTATAGTTTTTATTGACAAAAGCAAAGTAATCTTACAACTATG
TGCATACAAAAGCAATACTATTTTGTGACTAAATATTTTATATTAAAATTTACATCAGCA
ACTGTCTTGAGAATTCAGGGAAATAGAATGGAATTTAAAACTTCAACAGTTTTGTTAAAT
CTAGAAACATGAAATTAGTATTCCAAAGAGATTCTGAAATTTCTTTTCTTGGGGAAATGA
CGGTACATTAAATCAAAATTGAGGATGGATGATTTAAAAACATTTGACTTTTTAATAATA
AAAAGAAAAGTGAAGAGTAAGAGAAATTGTATTAGTTGTATGTTTCTTGTTTTTGTTTTT
AACTACTCCCTTTGGAATACCAGTTTGACTTGTAAGTGGTGGTTTCTAAATAAATACCTG
GCCAGAGGACCATCACCTGTCACCTTATTAAAACAGTATTTTTAAATAGGTTCTTTTTGT
TGTCTTAGATGTGATCAAATTATTTCAAGGAAAATGAAAATAATTCTACTTACCCTCACT
TTTTAAAGTGAAGAGTTTAGAACTTAAGAATATATTTTCAAATGTGAATAAAACTTCATA
TCCAGGAAAGGATGCTATTTTAAGTATCATATACTTTAAGAGTATCTAGCAAGCGCTGTG
TGCTTCTCTGTCTTGTCTTGTTTATTCCTCAGTAAATTACAATGGTGGTACCAAAAACAA
GACTATACTGCATAGTTGGATAGAAAACCAGAAAGTGTCAACTTGCTACAGTCATTAAAA
GTTTTATTATTGGCTGAAAAAAAAAAAAAAAAAA
Restriction Sites EcoRI-XbaI     
ACCN NM_001284280
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001284280.1, NP_001271209.1
RefSeq Size 4574 bp
RefSeq ORF 2463 bp
Locus ID 55671
UniProt ID Q6IN85
Gene Summary Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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