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pol (AC_000019) Virus Tagged ORF Clone
CAT＃: VC100078
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus 35], codon optimized for human cell expression, AP_000576
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CNY 18,050.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100078 represents NCBI reference of AP_000576 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTCGTACAGGCGCACGGGGCCGGTTCCTTCCACCCCGAGACAAGCGACACTCGGAGGCAATCCA CGCGGGGAGGCGCGAGAAAATCTTCACAGAGTGCAATTCCAGAGCCGGCCCGCGCTAGACGCCGCCGCGC GACCACAAGTCATGCCCCAGGCAGTGGTACTACCGCTGCCGCTCGAAGAGCCAGCCCCTCACCTCTTCTG TCAATGCAGGAAGCCCGCGAGCCCCCCCCAATCAAGCGGAAGAACAAGGGCACCGTTGTCGCTCCCAAGG GTCACGGCACACTCCAGGCTATCGACGTGTCCACTAATGAGCCCGTGGAGATCAAATACCACCTGAACCT TCCTCACGCCCTGGAAAAGATTATGCAGGTCAATCTGCTGTCTCTTCCCACAAATCTGTCCCCCCAGCGC CTCCGCACCCTGGATAGTAGTAGCCTTCGGGCACTGGTTCTTCAGCTCCGGCCTTCCAGGGCTGAGGTTT GGACATGCCTTCCACGGGGACTCGTGAGCATGACGACAATTGAGACCGAGGACGAGCAGGCCGACACTGC GAATATTGAGGAACATGAGATTCAGTCCCCAGGACTCGGTTTCCCCCTGAAGTTCCTGGTGAAGGGTTCC CAAGTCCAGCTTATTCACGAGATACAGCCTGTGCAGAGATGTGACTACTGCGGCAGATTGTACAAACATA AACATGAGTGCTCTGCACGGCGGCGGAATTTTTACTTTCATCACATCAATTCCCAGTCAAGTAACTGGTG GCAGGAAATACAGTTTTTCCCCATCGGGTCCCACCCAAGGACGGAAAGACTCTTCTTGACATATGATGTG GAGACTTACACGTGGATGGGCTCCTTCGGGAAGCAGCTTGTCCCCTTCATGTTGGTCATGAAATTGTCTG GAGAAGAGCGACTCGTCAACATGGCACACGACCTTGCAATAAAGCTTAAATGGGATAGGTGGCAGCAGGA TCCGCGGACCTTCTACTGTGTTACGCCGGAGAAGATGGCTATTGGACGCCACTTTCGCCAGTATCGGGAT CAGCTGCAGACTGCGTTGGCCGTCGATCTCTGGTCATCATTTCTGCACGCCAACCCACACCTGCATGAAT GGGCGCTGGAACACTACGCCCTGAGCGACCCTACGGATCTGACTTTCGATGAACTCAGTAAGCTGCCTCA CGTGCGGGGCACCCCACGATTTATCGAGCTGTACATTGTCGGACACAATATCAATGGATTTGATGAAATT GTCCTCGCAGCACAAGTGATAAATAATCGCGCAGAGGTGCCTCAGCCTTTCCGCATTACTAGAAACTTTA TGCCTAGAGCTGGGAAAATTCTGTTTAATGATGTGACGTTCGCTCTGCCCAACCCCGCCTACAAAAAGAG AGTCGATTTCCAGCTGTGGGAGCAAGGCGCATGTGACGATATGGACTTCAAATACCAGTTTCTGAAAGTT ATGGTAAGGGATACGTTCGCACTCACCCATGCCTCACTGAGGAAGGCTGCACAGGCCTACTCTCTGCCCG TAGAAAAAGGCTGTTGCCCTTATAAAGCCGTTAATCAGTTCTACATGCTGGGGTCATATAGAGCCGATAA GGACGGGTTCCCGCTTGAAGAGTACTGGAAGGATCGCGAGGAATACCTGTTGAACCGGGAGCTGTGGGAA AAAAAGAATCAGCCCGGATATGATATCATCCAGGAAACTCTGGACTATTGTGCACTTGATGTTCTCGTTA CCGCAGAGCTGGTTACTAAACTGCAGGAATCCTATGCTCATTTCGTCAGGGACAGCGTGGGACTTCCACA CGCCCACTTTAACATTTTTCAGAGACCGACTATAAGCTCCAACTCACACGCCATTTTCAGGCAAATCGTA TATCGCACCGAGAAGCCTAATAGGACAAACCTTGGCCCCGGGCTGTTGGCCCCAAGCCACGAGCTCTATG ACTACGTTCGGGCTTCTATAAGAGGAGGGCGCTGTTACCCTACTTATATCGGAATCTTTGAGGACCCCAT TTATGTTTACGACATCTGCGGCATGTACGCCTCCGCCCTGACCCACCCAATGCCTTGGGGCAGCCCGCTG AATCCGTACGAAAGAGCCCTGGCCGCCCGAGAATGGCAGATGGCTTTGGACGATCCTACCCCTATCTCTT ATTTCGACAAAGATCTGCTGCCCGGCATTTTCACTATGGATGCAGATCCCCCCGATGAGTTGATGCTGGA CCCCCTCCCACCGTTTTGTTCACGCAAGGGGGGACGCCTTTGCTGGACAAACGAGCCCTTGCGCGGTGAA GTGGCAACATCCGTAGATCTGATTACGCTGCATAACCGAGGTTGGCAGGTACGCATTGTGCCAGACGAAC TCACAACAATTTTTCCCGAATGGAAATGCCTGGCCCGAGAGTACGTGCAGCTCAATATAGCAGCTAAAGA GAGAGCAGACAAAGAGAAAAATCAGACAATGCGGTCCATCGCCAAGCTCCTTAGTAACGCTCTGTATGGG AGCTTCGCCACAAAATTGGACAATAAAAAAATTGTATTTAGCGACCAGATGGATGAAAACCTTATGAAAG GCATCTCCACCGGTACTGTGAACATTAAGAGTAGCAGCTTCCTTGAGACCGATAACCTGAGCGCCGAAGT AATGCCCGCTTTCGAACGGGAATACCTGCCCCAGCAGCTGACCTTTTTGGACTCAGATCCAGAGGACTCA GAGGAAGAGCAGAGAACCGCACCGTTTTATACTCCGCCAGCCGGGACCCCAGGACACGTTAAGTATACGT ACAAGCCCATTACTTTCCTGGATGTTGAGGAGGGGGATATGTGTCTGCACACACTCGAAAAAGTGGATCC CCTGGTGGACAACGACAGATACCCAAGCCATATAGCAAGTTTTGTCCTGGCGTGGACAAGAGCTTTCGTG AGCGAGTGGTCTGAGTTTCTGTACGAAGAGGATAGAGGCACCCCGCTGGAGAAGCGGCCGGTGAAGTCAG TGTACGGCGATACGGATTCACTGTTCGTAACACAGCGGGGCCATGAGCTCATGGAAACAAAAGGCAAGAA GCGGATCAAAAAATACGGGGGGAAGCTGGTGTTCGATCCAAACGAACCTGACCTCACCTGGTTGGTGGAG TGCGAAACCGTCTGCGCCAGCTGCGGAGCCGACGCATACTCACCTGAGAGTATCTTCCTGGCTCCTAAAC TGTACGCCCTTAAATGTCTTTATTGCCCAGTTTGCAAAAAAACATCTAAAGGTAAGCTGCGGGCAAAGGG GCACGCTGCCGAGGCACTTAACTACGAGCTTATGGTGAATTGCTACCTGGCTGATATACAGGGGGCCAAA CGACAGAAGTTTTCTACCAGCAGAATGTCATTGAAGAGAACTCTCGCATCAGCCCAAGCCGGTGCACACC CCTTTACAGTGACCGAAACTACCCTTACTCGCACACTCAGACCATGGAAAGATCGGACGCTGGCCGCCCT TGACGCCCACCGACTGATCCCCTATTCCCGCTCCCGCCCTAATCCACGGAACGAGGAAGTGTGTTGGATC GAAATGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100078 representing AP_000576
Red=Cloning sites Green=Tags MALVQAHGAGSFHPETSDTRRQSTRGGARKSSQSAIPEPARARRRRATTSHAPGSGTTAAARRASPSPLL SMQEAREPPPIKRKNKGTVVAPKGHGTLQAIDVSTNEPVEIKYHLNLPHALEKIMQVNLLSLPTNLSPQR LRTLDSSSLRALVLQLRPSRAEVWTCLPRGLVSMTTIETEDEQADTANIEEHEIQSPGLGFPLKFLVKGS QVQLIHEIQPVQRCDYCGRLYKHKHECSARRRNFYFHHINSQSSNWWQEIQFFPIGSHPRTERLFLTYDV ETYTWMGSFGKQLVPFMLVMKLSGEERLVNMAHDLAIKLKWDRWQQDPRTFYCVTPEKMAIGRHFRQYRD QLQTALAVDLWSSFLHANPHLHEWALEHYALSDPTDLTFDELSKLPHVRGTPRFIELYIVGHNINGFDEI VLAAQVINNRAEVPQPFRITRNFMPRAGKILFNDVTFALPNPAYKKRVDFQLWEQGACDDMDFKYQFLKV MVRDTFALTHASLRKAAQAYSLPVEKGCCPYKAVNQFYMLGSYRADKDGFPLEEYWKDREEYLLNRELWE KKNQPGYDIIQETLDYCALDVLVTAELVTKLQESYAHFVRDSVGLPHAHFNIFQRPTISSNSHAIFRQIV YRTEKPNRTNLGPGLLAPSHELYDYVRASIRGGRCYPTYIGIFEDPIYVYDICGMYASALTHPMPWGSPL NPYERALAAREWQMALDDPTPISYFDKDLLPGIFTMDADPPDELMLDPLPPFCSRKGGRLCWTNEPLRGE VATSVDLITLHNRGWQVRIVPDELTTIFPEWKCLAREYVQLNIAAKERADKEKNQTMRSIAKLLSNALYG SFATKLDNKKIVFSDQMDENLMKGISTGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLTFLDSDPEDS EEEQRTAPFYTPPAGTPGHVKYTYKPITFLDVEEGDMCLHTLEKVDPLVDNDRYPSHIASFVLAWTRAFV SEWSEFLYEEDRGTPLEKRPVKSVYGDTDSLFVTQRGHELMETKGKKRIKKYGGKLVFDPNEPDLTWLVE CETVCASCGADAYSPESIFLAPKLYALKCLYCPVCKKTSKGKLRAKGHAAEALNYELMVNCYLADIQGAK RQKFSTSRMSLKRTLASAQAGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLIPYSRSRPNPRNEEVCWI EMP TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | AC_000019 |
| ORF Size | 3579 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | AC_000019.1, AP_000576 |
| RefSeq ORF | 3579 bp |
| MW | 136.3 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100073 | Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus 35], codon optimized for human cell expression, AP_000571 |
CNY 3,800.00 |
|
| VC100074 | Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus 35], codon optimized for human cell expression, AP_000572 |
CNY 3,800.00 |
|
| VC100075 | Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 35], codon optimized for human cell expression, AP_000573 |
CNY 5,990.00 |
|
| VC100076 | Myc-DDK-tagged ORF clone of viral ORF for IX [Human adenovirus 35], codon optimized for human cell expression, AP_000574 |
CNY 3,800.00 |
|
| VC100077 | Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 35], codon optimized for human cell expression, AP_000575 |
CNY 5,510.00 |
|
| VC100079 | Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 35], codon optimized for human cell expression, AP_000577 |
CNY 7,890.00 |
|
| VC100080 | Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 35], codon optimized for human cell expression, AP_000578 |
CNY 4,660.00 |
|
| VC100081 | Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 35], codon optimized for human cell expression, AP_000579 |
CNY 7,030.00 |
|
| VC100082 | Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 35], codon optimized for human cell expression, AP_000580 |
CNY 6,750.00 |
|
| VC100083 | Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 35], codon optimized for human cell expression, AP_000581 |
CNY 3,800.00 |
|
| VC100084 | Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 35], codon optimized for human cell expression, AP_000582 |
CNY 4,180.00 |
|
| VC100085 | Myc-DDK-tagged ORF clone of viral ORF for pX [Human adenovirus 35], codon optimized for human cell expression, AP_000583 |
CNY 3,800.00 |
|
| VC100086 | Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 35], codon optimized for human cell expression, AP_000584 |
CNY 3,800.00 |
|
| VC100087 | Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 35], codon optimized for human cell expression, AP_000585 |
CNY 14,350.00 |
|
| VC100088 | Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus 35], codon optimized for human cell expression, AP_000586 |
CNY 3,800.00 |
|
| VC100089 | Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 35], codon optimized for human cell expression, AP_000587 |
CNY 6,270.00 |
|
| VC100090 | Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 35], codon optimized for human cell expression, AP_000588 |
CNY 12,350.00 |
|
| VC100091 | Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 35], codon optimized for human cell expression, AP_000589 |
CNY 3,800.00 |
|
| VC100092 | Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 35], codon optimized for human cell expression, AP_000590 |
CNY 3,800.00 |
|
| VC100093 | Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 35], codon optimized for human cell expression, AP_000591 |
CNY 3,800.00 |
|
| VC100094 | Myc-DDK-tagged ORF clone of viral ORF for E3 125K [Human adenovirus 35], codon optimized for human cell expression, AP_000592 |
CNY 3,800.00 |
|
| VC100095 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha0 [Human adenovirus 35], codon optimized for human cell expression, AP_000593 |
CNY 3,800.00 |
|
| VC100096 | Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus 35], codon optimized for human cell expression, AP_000594 |
CNY 3,800.00 |
|
| VC100097 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-beta1 [Human adenovirus 35], codon optimized for human cell expression, AP_000595 |
CNY 3,800.00 |
|
| VC100098 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-gamma1 [Human adenovirus 35], codon optimized for human cell expression, AP_000596 |
CNY 3,800.00 |
|
| VC100099 | Myc-DDK-tagged ORF clone of viral ORF for E3 RID-alpha [Human adenovirus 35], codon optimized for human cell expression, AP_000597 |
CNY 3,800.00 |
|
| VC100100 | Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus 35], codon optimized for human cell expression, AP_000598 |
CNY 3,800.00 |
|
| VC100101 | Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus 35], codon optimized for human cell expression, AP_000599 |
CNY 3,800.00 |
|
| VC100102 | Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus 35], codon optimized for human cell expression, AP_000600 |
CNY 3,800.00 |
|
| VC100103 | Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 35], codon optimized for human cell expression, AP_000601 |
CNY 3,800.00 |
|
| VC100104 | Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus 35], codon optimized for human cell expression, AP_000603 |
CNY 3,800.00 |
|
| VC100105 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF6/7 [Human adenovirus 35], codon optimized for human cell expression, AP_000602 |
CNY 3,800.00 |
|
| VC100106 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus 35], codon optimized for human cell expression, AP_000604 |
CNY 3,800.00 |
|
| VC100107 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF3 [Human adenovirus 35], codon optimized for human cell expression, AP_000605 |
CNY 3,800.00 |
|
| VC100108 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF2 [Human adenovirus 35], codon optimized for human cell expression, AP_000606 |
CNY 3,800.00 |
|
| VC100109 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF1 [Human adenovirus 35], codon optimized for human cell expression, AP_000607 |
CNY 3,800.00 |
